International audience | The common mussel Mytilus galloprovincialis was selected as unique biomonitor species to implement a regional monitoring programme, the CIESM Mediterranean Mussel Watch (MMW), in the Mediterranean and Black Seas. As of today, and upon standardization of the methodological approach, the MMW Network has been able to quantify 137Cs levels in mussels from 60 coastal stations and to produce the first distribution map of this artificial radionuclide at the scale of the entire Mediterranean and Black Seas. While measured 137Cs levels were found to be very low (usually <1 Bq kg-1 wet wt) 137Cs activity concentrations in the Black Sea and North Aegean Sea were up to two orders of magnitude higher than those in the western Mediterranean Basin. Such effects, far from representing a threat to human populations or the environment, reflect a persistent signature of the Chernobyl fallout in this area. © 2007 Elsevier Ltd. All rights reserved.

Quantitative identification of the main sources of heavy metals and polycyclic aromatic hydrocarbons (PAHs) in soils around multiple types of key areas is of great significance for blocking pollution sources. However, there is a lack of more comprehensive relevant research. In this study, Beijing was taken as the research area and four main sources were identified using the positive matrix factorization (PMF) method. The concentration of Pb, PAHs, Cr, and Hg in soils was significantly affected by the presence of landuse type, road traffic, natural factor, and industrial production, respectively, and the farmland, distance to main road, Proterozoic Changcheng-Jixian parent material and cinnamon soil type, and the gross industrial production make greater contributions to these four factors respectively than other variables. Moreover, the uncertainty of the PMF indicates that this four-factor PMF solution is stable and appropriate. These results provide support for the comprehensive control of soil environmental risks.

Owing to the production and use of chemicals in chemical industry parks (CIPs), these areas are considered to be highly polluted. However, the type of pollutants presents in the wastewater from CIPs and the risk posed to the environment due to the release of these pollutants remains unclear. In this study, suspect screening was combined with traceability analysis to determine the type of pollutants present in wastewaters at 9 chemical enterprises and wastewater treatment plants (WWTPs) in the CIPs. Additionally, the distribution of nine pollutants from the WWTPs’ effluent stage and the risk they posed to the surrounding river was examined through target analysis. Upon conducting suspect analysis, the presence of 65 and 64 chemicals in the 9 chemical enterprises’ wastewaters and WWTPs, respectively, was tentatively identified. Traceability analysis of the compounds screened in the effluent from the WWTPs determined that 41 substances were identified as characteristic pollutants of the chemical enterprises, indicating that the suspect screening strategy enabled relatively more efficient identification of the characteristic pollutants compared to traditional quantitative analysis. Targeting analysis combined with ecological risk assessment showed that metolachlor, carbendazim, atrazine, diuron, and chlorpyrifos posed relatively higher risks to aquatic organisms in the surrounding river. Therefore, the refined management of the wastewater treatment plant in the CIPs is necessary.

Di (2-ethylhexyl) phthalate (DEHP), a widely spreading environmental endocrine disruptor, has been confirmed to adversely affect the development of animals and humans. The formation of neutrophil extracellular traps (NETs) termed NETosis, is a recently identified antimicrobial mechanism for neutrophils. Though previous researches have investigated inescapable role of the immunotoxicity in DEHP-exposed model, relatively little is known about the effect of DEHP on NETs. In this study, carp peripheral blood neutrophils were treated with 40 and 200 μmol/L DEHP to investigate the underlying mechanisms of DEHP-induced NETs formation. Through the morphological observation of NETs and quantitative analysis of extracellular DNA, we found that DEHP exposure induced NETs formation. Moreover, our results proved that DEHP could increase reactive oxygen species (ROS) levels, decrease the expression of the anti-autophagy factor (mTOR) and the anti-apoptosis gene Bcl-2, and increase the expression of pro-autophagy genes (Dynein, Beclin-1 and LC3B) and the pro-apoptosis factors (BAX, Fas, FasL, Caspase3, Caspase8, and Caspase9), thus promoting autophagy and apoptosis. These results indicate that DEHP-induced ROS burst stimulates NETs formation mediated by autophagy and increases apoptosis in carp neutrophils.

Southwestern China contains the largest and most well-developed karst region in the world, and the potentially toxic elements (PTEs) content in the soils of the region is remarkably high. To explore the internal and external control factors and sources of soil PTEs enrichment in this area and to provide a basis for the treatment of PTE pollution, 113 soil samples were collected from Hengxian County, a karst region in Guangxi Province, southwestern China. The importance of eighteen influencing factors including parent material, weathering, physicochemical properties, topography and human activities were quantitatively analyzed by (partial) redundancy analysis. The sources of PTEs were identified using the Pb isotope ratio and absolute principal component score/multiple linear regression (APCS-MLR) model. The contents of all soil PTEs were higher than the corresponding background values of Guangxi soils. The contents in Cu, Zn, Cd, Hg and Pb were the highest in the soil from carbonate rock. The factor group of geological background and weathering explained 26.5% for the accumulation and distribution of soil PTEs and the influence of physicochemical properties was less than 2% but increased to 25.6% through interaction with weathering. Fe (47.1%), Al (42.1%), Mn (22%), chemical index of alteration (12.8%) and clay (11.9%) were the key factors affecting the soil PTEs, while the influence of human activities was weak. Pb isotope ratio and APCS-MLR classified 62.8–74% of soil PTEs as derived from natural sources, whereas 18.23% and 18.95% were derived from industrial activities and agricultural practice/traffic emissions, respectively. The Pb isotope ratio showed that the natural sources account for up to 90% of the Pb in the soil from carbonate rock, the highest contribution among the studied soils. The results of the study can provide background information on the soil PTEs contamination in the karst areas of China and other areas worldwide.

We used the Weather Research and Forecasting Model coupled with Chemistry (WRF-Chem) to simulate elemental carbon (EC) concentrations in Thailand in 2017. The goals were to quantify the respective contributions of local emissions and regional transport outside Thailand to EC pollution in Thailand, and to identify the most effective emission control strategy for decreasing EC pollution. The simulated EC concentrations in Chiang Mai, Bangkok, and Phuket were comparable with the observation data. The correlation coefficient between the simulated and observed EC concentrations was 0.84, providing a good basis for evaluating EC sources in Thailand. The simulated mean EC concentration over the whole country was the highest (1.38 μg m⁻³) in spring, and the lowest (0.51 μg m⁻³) in summer. We conducted several sensitivity simulations to evaluate EC sources. Local emissions (including anthropogenic and biomass burning emissions) and regional transport outside Thailand contributed 81.2% and 18.8% to the annual mean EC concentrations, respectively, indicating that local sources played the dominant role for EC pollution in Thailand. Among the local sources, anthropogenic emissions (including the industry, power plant, residential, and transportation sectors) and biomass burning contributed 75.1% and 6.1% to the annual mean EC concentrations, respectively. As the anthropogenic emissions dominated the EC pollution, we performed four sensitivity simulations by reducing 30% of the emissions from each of the industry, power plant, residential, and transportation sectors in Thailand. The results indicated that controlling transportation emissions in Thailand was the most effective way in reducing the EC pollution. The 30% reduction of transportation emissions decreased the annual mean EC concentrations by 12.1%. In contrast, 30% reductions of the residential, industry, and power plant emissions caused 8.4%, 6.4%, and 4.0% decreases in the annual mean EC concentrations, respectively. The model results could potentially provide useful information for air pollution control strategies in Thailand.

Condensed organic matters (COM) with black carbon-like structures are considered as long-term carbon sinks because of their high stability. It is difficult to distinguish COM from general organic matter by conventional chemical analysis, thus the contribution by and interaction mechanisms of organo-mineral complexes in COM stabilization are unclear and generally neglected. Molecular markers related to black carbon-like structures, such as benzene polycarboxylic acids (BPCAs), are promising tools for the qualitative and quantitative analysis of COM. In this study, one natural soil and two cultivated soils with 25 y- or 55 y-tillage activities were collected and the distribution characteristics of BPCAs were detected. All the investigated soils showed similar BPCA distribution pattern, and over 60% of BPCAs were detected in clay fraction. The extractable BPCA contents were substantially increased after mineral removal. The ratios of BPCA contents before and after mineral removal indicate the extent of COM-mineral particle interactions, and our results suggested that up to 73% COM were protected by mineral particles, and more stronger interactions were noted on clay than on silt. The initial cultivation dramatically decreased COM-clay interactions, and this interaction was recovered only slowly after 55-y cultivation. Kaolinite and muscovite are important for COM protection. But a possible negative correlation between BPCAs and reactive iron oxides of the cultivated soils suggested that iron may promote COM degradation when disturbed by tillage activities. This study provided a new angle to study the stabilization of COM and emphasized the importance of organo-mineral complexes for COM stabilization.

As a strong reductant and highly active alkali, hydrazine (N2H4) has been widely used in chemical industry, pharmaceutical manufacturing and agricultural production. However, its high acute toxicity poses a threat to ecosystem and human health. In the present study, a ratiometric fluorescent probe for the detection of N2H4 was designed, utilizing dicyanoisophorone as the fluorescent group and 4-bromobutyryl moiety as the recognition site. 4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-enyl) phenyl 4-brobutanoate (DDPB) was readily synthesized and could specially sense N2H4 via an intramolecular charge transfer (ICT) pathway. The cyclization cleavage reaction of N2H4 with a 4-bromobutyryl group released phenolic hydroxyl group and reversed the ICT process between hydroxy group and fluorophore, turning on the fluorescence in the DDPB-N2H4 complexes. DDPB exhibits a low cytotoxicity, reasonable cell permeability, a large Stokes shift (186 nm) and a low detection limit (86.3 nM). The quantitative determination of environmental water systems and the visualization fluorescence of DDPB test strips provides a strong evidence for the applications of DDPB. In addition, DDPB is suitable for the fluorescence imaging of exogenous N2H4 in HeLa cells and zebrafish.

Mangroves act as sinks for terrigenous pollutants to alleviate their influence on offshore marine ecosystem. The nationwide study of PBDEs contamination in mangrove wetlands of China has not been explored, and their risk for human health lack quantitative analysis. In this study, sediment samples were collected in six mangrove wetlands along coastal area of South China to evaluate the levels, congener distributions and ecological risks of eight PBDEs, including BDE-28, -47, −99, −100, −153, −154, −183, and −209. Levels of ∑PBDEs (the sum of seven PBDEs except BDE-209) and BDE-209 were 0.13–2.18 ng g−1 and 1.44–120.28 ng g−1, respectively. In particular, mean level of BDE-209 was highest in Futian, followed by Yunxiao, Fangchenggang, Zhanjiang, Dongzhaigang, and Dongfang. As dominant PBDE congener, BDE-209 accounted for 63.6%–99.1% of the total PBDEs, suggesting the major sources of commercial deca-BDE mixtures. Among seven PBDE congeners except BDE-209, slightly different percentages of PBDE congeners were detected, with BDE-154, -47, and −100 being predominant congeners. Positive relationship was observed for total organic matter (TOM) with BDE-209, with no such relationships found for particle size compositions (clay, silt and sand). As for sediment-dwelling organism, the ecological risks from tri-, tera-, and hexa-BDE congeners could be negligible, and those from penta- and deca-BDE congeners were low or moderate, indicating major ecological risk drivers of penta- and deca-BDE congeners in mangrove wetlands in China. The ecological risk of PBDEs in mangrove sediments for human health was thought to be consumption of fish which would bioaccumulate PBDEs from the contaminated sediment. As for human health, the levels of non-cancer risks of PBDEs were all lower than 1, and the cancer risk was far less than the threshold level (10−6), demonstrating low risk for human health.

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants with a number of them being carcinogenic. One of the approaches to assess human exposure to PAHs is to measure their urinary metabolites, monohydroxyl polycyclic aromatic hydrocarbons (OH-PAHs), with a method allowing for high throughput and short turn-around time. We developed a method to quantify nine urinary OH-PAHs by using supported liquid phase extraction (SLE) and isotope dilution gas chromatography tandem mass spectrometry (GC-MS/MS). SLE demonstrated advantages over the traditionally used liquid-liquid extraction techniques. The target analytes with spiked deuterated and ¹³C-labeled internal standards were extracted from urine by SLE after enzymatic cleavage of the glucuronide and sulfate conjugates. The extracted analytes were then derivatized with N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and analyzed by GC-MS/MS. Six solvent mixtures were evaluated as the SLE extraction solvent, and pentane:chloroform (7:3, v/v) was selected due to its best overall analytical performance. Method detection limits for the 9 analytes ranged from 2.3 to 13.8 pg/mL. Precision and accuracy were satisfactory. SLE and internal isotope labeled standard combination reduced matrix effect effectively. This new method using SLE sample preparation techniques coupled with GC-MS/MS proves applicable to urinary measurements for PAH exposure studies for general population.