International audience | Telomeres are non-coding DNA repeats located at the termini of eukaryotic chromosomes, regulated by dynamic processes balancing shortening and maintenance. Despite a mechanism to slow-down telomere shortening, cell division leads to progressive attrition of chromosomes, leading to the onset of cellular senescence or apoptosis. However, telomere restoration based on telomerase activity is the primary mechanism for telomere maintenance. Telomere length is associated to health and survival and can be impacted by a broad panel of environmental factors. However, the effect of contaminants on telomeres is poorly known for living organisms. The aim of this study was to investigate relationships between some poly- and perfluoroalkyl substances (PFASs), body condition and telomere length by using both a cross-sectional and longitudinal approach in adult breeding Black-legged kittiwakes (Rissa tridactyla) from Svalbard. First, we examined the associations between absolute telomere length and PFASs contamination in a given year (cross-sectional approach). Second, we investigated the relationships between telomere dynamics and PFASs contamination within a two years’ time frame (longitudinal approach). Our results did not show any significant relationships of PFASs and body condition with absolute telomere length in a given year. Surprisingly, we found a positive and significant relationship between PFASs and telomere dynamics in both sexes with elongated telomere in birds bearing the highest concentrations of PFASs. Our study underlines (i) the need to investigate PFAS effects on telomere dynamics with a longitudinal approach and (ii) a potential positive effect of these contaminants on telomere length, with the most contaminated birds showing the slowest rate of telomere shortening or even displaying elongated ones. Our study is the first to report a relationship between PFASs and telomere length in free-living vertebrates. A possible underlying mechanism and other potential confounding factors are discussed.

Telomeres are non-coding DNA repeats located at the termini of eukaryotic chromosomes, regulated by dynamic processes balancing shortening and maintenance. Despite a mechanism to slow-down telomere shortening, cell division leads to progressive attrition of chromosomes, leading to the onset of cellular senescence or apoptosis. However, telomere restoration based on telomerase activity is the primary mechanism for telomere maintenance. Telomere length is associated to health and survival and can be impacted by a broad panel of environmental factors. However, the effect of contaminants on telomeres is poorly known for living organisms. The aim of this study was to investigate relationships between some poly- and perfluoroalkyl substances (PFASs), body condition and telomere length by using both a cross-sectional and longitudinal approach in adult breeding Black-legged kittiwakes (Rissa tridactyla) from Svalbard. First, we examined the associations between absolute telomere length and PFASs contamination in a given year (cross-sectional approach). Second, we investigated the relationships between telomere dynamics and PFASs contamination within a two years’ time frame (longitudinal approach). Our results did not show any significant relationships of PFASs and body condition with absolute telomere length in a given year. Surprisingly, we found a positive and significant relationship between PFASs and telomere dynamics in both sexes with elongated telomere in birds bearing the highest concentrations of PFASs. Our study underlines (i) the need to investigate PFAS effects on telomere dynamics with a longitudinal approach and (ii) a potential positive effect of these contaminants on telomere length, with the most contaminated birds showing the slowest rate of telomere shortening or even displaying elongated ones. Our study is the first to report a relationship between PFASs and telomere length in free-living vertebrates. A possible underlying mechanism and other potential confounding factors are discussed.

Telomeres are non-coding DNA repeats located at the termini of eukaryotic chromosomes, regulated by dynamic processes balancing shortening and maintenance. Despite a mechanism to slow-down telomere shortening, cell division leads to progressive attrition of chromosomes, leading to the onset of cellular senescence or apoptosis. However, telomere restoration based on telomerase activity is the primary mechanism for telomere maintenance. Telomere length is associated to health and survival and can be impacted by a broad panel of environmental factors. However, the effect of contaminants on telomeres is poorly known for living organisms. The aim of this study was to investigate relationships between some poly- and perfluoroalkyl substances (PFASs), body condition and telomere length by using both a crosssectional and longitudinal approach in adult breeding Black-legged kittiwakes (Rissa tridactyla) from Svalbard. First, we examined the associations between absolute telomere length and PFASs contamination in a given year (cross-sectional approach). Second, we investigated the relationships between telomere dynamics and PFASs contamination within a two years’ time frame (longitudinal approach). Our results did not show any significant relationships of PFASs and body condition with absolute telomere length in a given year. Surprisingly, we found a positive and significant relationship between PFASs and telomere dynamics in both sexes with elongated telomere in birds bearing the highest concentrations of PFASs. Our study underlines (i) the need to investigate PFAS effects on telomere dynamics with a longitudinal approach and (ii) a potential positive effect of these contaminants on telomere length, with the most contaminated birds showing the slowest rate of telomere shortening or even displaying elongated ones. Our study is the first to report a relationship between PFASs and telomere length in freeliving vertebrates. A possible underlying mechanism and other potential confounding factors are discussed. Organic contaminants PFASs Black-legged kittiwake Svalbard DNA | acceptedVersion

Telomeres are non-coding DNA repeats located at the termini of eukaryotic chromosomes, regulated by dynamic processes balancing shortening and maintenance. Despite a mechanism to slow-down telomere shortening, cell division leads to progressive attrition of chromosomes, leading to the onset of cellular senescence or apoptosis. However, telomere restoration based on telomerase activity is the primary mechanism for telomere maintenance. Telomere length is associated to health and survival and can be impacted by a broad panel of environmental factors. However, the effect of contaminants on telomeres is poorly known for living organisms. The aim of this study was to investigate relationships between some poly- and perfluoroalkyl substances (PFASs), body condition and telomere length by using both a crosssectional and longitudinal approach in adult breeding Black-legged kittiwakes (Rissa tridactyla) from Svalbard. First, we examined the associations between absolute telomere length and PFASs contamination in a given year (cross-sectional approach). Second, we investigated the relationships between telomere dynamics and PFASs contamination within a two years’ time frame (longitudinal approach). Our results did not show any significant relationships of PFASs and body condition with absolute telomere length in a given year. Surprisingly, we found a positive and significant relationship between PFASs and telomere dynamics in both sexes with elongated telomere in birds bearing the highest concentrations of PFASs. Our study underlines (i) the need to investigate PFAS effects on telomere dynamics with a longitudinal approach and (ii) a potential positive effect of these contaminants on telomere length, with the most contaminated birds showing the slowest rate of telomere shortening or even displaying elongated ones. Our study is the first to report a relationship between PFASs and telomere length in freeliving vertebrates. A possible underlying mechanism and other potential confounding factors are discussed. Organic contaminants PFASs Black-legged kittiwake Svalbard DNA | acceptedVersion

International audience | To support environmental management programs, there is an urgent need to know about the presence and understand the dynamics of major contaminants in seabird communities of key marine ecosystems. In this study, we investigated the concentrations and trophodynamics of trace elements in six seabird species and persistent organic pollutants (POPs) in three seabird species breeding on Grand Connétable Island (French Guiana), an area where the increase in human population and mining activities has raised concerns in recent years. Red blood cell Hg concentrations in adults were the highest in Magnificent frigatebirds Fregata magnificens (median: 5.6 μg g−1 dw; range: 3.8-7.8 μg g−1 dw) and lowest in Sooty terns Onychoprion fuscatus (median: 0.9 μg g−1 dw; range: 0.6-1.1 μg g−1 dw). Among POPs, dichlorodiphenyldichloroethylene (p,p’-DDE) was the most abundant compound in plasma of Cayenne terns Thalasseus sandvicensis (median: 1100 pg g−1 ww; range: 160±5100 pg g−1 ww), while polychlorinated biphenyls (PCBs) were the most abundant compound class in plasma of Magnificent frigatebirds (median: 640 pg g−1 ww; range 330±2700 pg g−1 ww). While low intensity of POP exposure does not appear to pose a health threat to this seabird community, Hg concentration in several adults Laughing gulls Leucophaeus atricilla and Royal terns Thalasseus maximus, and in all Magnificent frigatebirds was similar or higher than that of high contaminated seabird populations. Furthermore, nestling red blood cells also contained Hg concentrations of concern, and further studies should investigate its potential health impact in this seabird community. Differences in adult trophic ecology of the six species explained interspecific variation in exposure to trace element and POPs, while nestling trophic ecology provides indications about the diverse feeding strategies adopted by the six species, with the consequent variation in exposure to contaminants.

To support environmental management programs, there is an urgent need to know about the presence and understand the dynamics of major contaminants in seabird communities of key marine ecosystems. In this study, we investigated the concentrations and trophodynamics of trace elements in six seabird species and persistent organic pollutants (POPs) in three seabird species breeding on Grand Connétable Island (French Guiana), an area where the increase in human population and mining activities has raised concerns in recent years. Red blood cell Hg concentrations in adults were the highest in Magnificent frigatebirds Fregata magnificens (median: 5.6 μg g−1 dw; range: 3.8–7.8 μg g−1 dw) and lowest in Sooty terns Onychoprion fuscatus (median: 0.9 μg g−1 dw; range: 0.6–1.1 μg g−1 dw). Among POPs, dichlorodiphenyldichloroethylene (p,p’-DDE) was the most abundant compound in plasma of Cayenne terns Thalasseus sandvicensis (median: 1100 pg g−1 ww; range: 160 ± 5100 pg g−1 ww), while polychlorinated biphenyls (PCBs) were the most abundant compound class in plasma of Magnificent frigatebirds (median: 640 pg g−1 ww; range 330 ± 2700 pg g−1 ww). While low intensity of POP exposure does not appear to pose a health threat to this seabird community, Hg concentration in several adults Laughing gulls Leucophaeus atricilla and Royal terns Thalasseus maximus, and in all Magnificent frigatebirds was similar or higher than that of high contaminated seabird populations. Furthermore, nestling red blood cells also contained Hg concentrations of concern, and further studies should investigate its potential health impact in this seabird community. Differences in adult trophic ecology of the six species explained interspecific variation in exposure to trace element and POPs, while nestling trophic ecology provides indications about the diverse feeding strategies adopted by the six species, with the consequent variation in exposure to contaminants.

[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA | International audience | This study evaluates the combined effects of two contaminants (copper and a herbicide S-metolachlor) and temperature on the early life stages of the Pacific oyster Crasssotrea gigas,which is native to Arcachon Bay (southwest France). The responses of D-larvae, obtained fromwild and cultivated oysters,were investigated for one year during the oyster breeding period at different sampling sites and compared with the response of D-larvae from a commercial hatchery. Embryotoxicity was measured by considering the percentage of abnormal D-shaped larvae. Normal development of embryos occurred only from 22 °C to 26 °C. There were synergistic effects of copper at low and high temperatures. Native oysters appear particularly sensitive to an increase in water temperature, suggesting a future increase in the percentage of larval abnormalities as a result of global climate change. Hatchery oysters represent a good alternative model for studying the effects of both pollutants and climate change stressors.

This study evaluates the combined effects of two contaminants (copper and a herbicide S-metolachlor) and temperature on the early life stages of the Pacific oyster Crasssotrea gigas, which is native to Arcachon Bay (southwest France). The responses of D-larvae, obtained from wild and cultivated oysters, were investigated for one year during the oyster breeding period at different sampling sites and compared with the response of D-larvae from a commercial hatchery. Embryotoxicity was measured by considering the percentage of abnormal D-shaped larvae. Normal development of embryos occurred only from 22°C to 26°C. There were synergistic effects of copper at low and high temperatures. Native oysters appear particularly sensitive to an increase in water temperature, suggesting a future increase in the percentage of larval abnormalities as a result of global climate change. Hatchery oysters represent a good alternative model for studying the effects of both pollutants and climate change stressors.