Heavy metal contamination is widespread in Bangladesh. Previous studies have observed lead increases blood pressure over time. However, the role of other metal contaminants and essential micronutrients, which could also adversely affect blood pressure or act as protective factors, is understudied.We therefore evaluated the associations of lead, manganese, and selenium with blood and pulse pressure trajectories.We prospectively followed placebo-assigned participants nested within a randomized trial for the prevention of arsenic-related skin cancer (n = 255). Blood lead, manganese, and selenium were measured at baseline; blood pressure was measured at baseline and at 3 biennial follow-up examinations. Mixed-effect linear regression models were used to estimate associations with average annual changes in systolic, diastolic, and pulse pressure.In models simultaneously adjusted for baseline blood lead, manganese, and selenium concentrations in addition to other potential confounders, lead was linearly associated with increases in systolic blood pressure, but not with diastolic blood pressure or pulse pressure. A non-linear association was observed for manganese, such that mid-range concentrations were associated with decreases in systolic, diastolic, and pulse pressure. Baseline selenium concentrations in the highest quartile were also associated with longitudinal decreases in both systolic and diastolic blood pressure, while null associations were observed with pulse pressure. In exploratory analyses, the combination of mid-range manganese and high selenium concentrations completely offset lead-associated increases in blood and pulse pressure.The results indicate a direct, linear association of lead exposure with systolic blood pressure, and manganese and selenium exposures within certain ranges may have a blood pressure-lowering effect in this population.

To monitor the body burden of persistent organic pollutants in mothers and infants living in proximity to a 10-year-old municipal waste incinerator (MWI), levels of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) in the breast milk of mothers were evaluated, and the estimated daily intake (EDI) level of these pollutants in infants was assessed. In total, 14 mothers from the exposure area and 18 mothers from a control area were recruited for this study. In addition, the levels of 17 PCDD/F congeners and 18 PCB congeners in the breast milk of the mothers were estimated, and risk assessment for their infants by calculating their EDI levels was performed. The mean levels of ΣPCDD/Fs and TEQ-ΣPCDD/Fs in the breast milk of the exposure group were significantly greater than those of the control group (3.36 vs. 1.47 pg/g wet weight; and 0.24 vs. 0.12 pg TEQ/g wet weight, respectively; p < 0.05). With respect to PCBs, the mean levels of the total PCBs (ΣPCBs) and non-dioxin like-PCBs (ΣNDL-PCBs) in the exposure group were significantly greater than those in the control group (269 vs. 141 pg/g wet weight; 230 vs. 115 pg/g wet weight, respectively; p < 0.05). The mean levels of ΣPCDD/Fs and TEQ-ΣPCDD/Fs were positively correlated with the inhabit time of the exposure group (r = 0.636, r = 0.629, p < 0.05, respectively). The mean EDI level of the exposure group in infants was significantly greater than that of the control group (22.0 vs. 13.0 pg TEQ/kg bw day; p < 0.05). In conclusion, mothers and their breast-fed infants living in proximity to the 10-year-old MWI located in the Zhejiang Province of China exhibited a significantly higher body burden of PCDD/Fs and PCBs compared to those living in the control area.

This study examined the influences of three subsets of environmental factors (i.e. soil physicochemical properties including pH, organic matters and soil texture, landscape patterns, and parent materials) on the spatial variations and sources of soil trace metal contamination across an urban-rural environmental gradient in Guangzhou City, southern China. We collected 318 surface soil samples from forests, orchards, farmlands, and urban lawns using a random tessellation design for selecting sample sites. The geo-accumulation indices showed that 18%–88% of soil samples were contaminated: moderate to high contamination with Cd and Hg, low to moderate contamination with Cu, Pb, Zn and Ni, and low contamination with As and Cr. However, less than 13% of soil samples were considered to have exceeded the national standards causing environmental and human health concerns. The mean geo-accumulation indices increased in the order of forest, paddy field/orchard, vegetable, road/residential, and park/residential areas for As, Cd, Ni, Pb, Zn, closely following a land disturbance gradient. Spearman Correlation and Cluster Analyses showed that Pb-Cu-Zn had traffic-related origins, Cd-Hg were mainly influenced by fertilization or industrial emissions, and As-Cr-Ni had geogenic origins for agricultural soils. In contrast, the Ni, Hg and Cd contamination sources for urban soils included both anthropogenic and geogenic origins. The Stepwise Regression and Partial Redundancy Analyses showed that three subsets of environmental factors explained 43%–87% of variations of soil contamination for both agricultural and urban soils. We concluded that soil contamination was mainly controlled by soil physiochemical properties followed by landscape patterns. Soil absorption of aerial loads of trace metal pollutants dominated the soil contamination processes. Our findings implied that improving soil physiochemical properties and landscape designs can strengthen environmental buffering and carrying capacity, thus alleviating soil contamination and reducing non-point-source pollution in the study region.

Previous studies have suggested a change of birth weight linked with elevated ambient air pollutant concentrations during the pregnancy. However, investigations of the influence of higher pollutant levels on birth weight change are limited. The goal of this study is to evaluate whether the air pollution of Ningbo is associated with birth weight, and which trimester could be a window period for maternal exposure to air pollution. A total of 170,008 live births were selected in the Ningbo city of Zhejiang, China, from 2015 to 2017. We estimated the association between the decreased birth weight and the increased air pollutant concentrations in the three trimesters and full gestation. The effects of interaction among pollutants were identified using a co-pollutant adjustment model. An interquartile range increases in PM2.5 (10.55 μg/m3), SO2(4.6 μg/m3), CO (125.59 μg/m3), and O3 (14.54 μg/m3) concentrations during the entire gestation were associated with 3.65 g (95% confidence interval: −6.02 g, −1.29 g), 5.02 g (−6.89 g, −3.14 g), 2.64 g (−4.65 g, −0.63 g) and 2.9 g (−4.8 g, 1 g) decreases, respectively, in birth weight. With each interquartile range increment in NO2 concentration was associated with an 8.05 g (6.24 g, 9.85 g) increase in birth weight. In the first trimester, only the PM2.5 exposure seemed to be associated with the greatest decline in birth weight. After adjustment for co-pollutant, both PM2.5 and SO2 were still associated with birth weight, except for CO for O3 adjustment, O3 for SO2 adjustment, and O3 for NO2 adjustment. Maternal exposure to air pollution may be associated with a decrease of birth weight, but the contribution of various pollutants is necessary to verify by future research.

This study investigated the effects of pollution emissions on the bioreactivity of PM2.5 during Asian dust periods. PM2.5 during the sampling period were 104.2 and 85.7 μg m−3 in Xi'an and Beijing, respectively, whereas PM2.5 which originated from the Tengger Desert was collected (dust background). Pollution conditions were classified as non-dust days, pollution episode (PE), dust storm (DS)-1, and DS-2 periods. We observed a significant decrease in cell viability and an increase in LDH that occurred in A549 cells after exposure to PM2.5 during a PE and DS-1 in Xi'an and Beijing compared to Tengger Desert PM2.5. Positive matrix factorization was used to identify pollution emission sources. PM2.5 from biomass and industrial sources contributed to alterations in cell viability and LDH in Xi'an, whereas vehicle emissions contributed to LDH in Beijing. OC, EC, Cl−, K+, Mg2+, Ca, Ti, Mn, Fe, Zn, and Pb were correlated with cell viability and LDH for industrial emissions in Xi'an during DS. OC, EC, SO42−, S, Ti, Mn, and Fe were correlated with LDH for vehicle emissions in Beijing during DS. In conclusion, the dust may carry pollutants on its surface to downwind areas, leading to increased risks of particle toxicity.

Nowadays, the occurrence of a large volume of plastic litter in oceanic and coastal zones has increased concern about its impacts on marine organisms. The degradation of plastic polymers leads to the formation of smaller fragments at both micro and nano scale (<5 mm and <1 μm respectively). Nanoplastics (NPs), due to their smaller size and high specific surface area can establish colloidal interactions with marine microalgae, therefore potential toxicity can be led. . To assess this hypothesis, the aim of the present study is to examine the behaviour of polystyrene nanoparticles (PS NPs) of different sizes (50 and 100 nm) in marine water and their possible effects at different physiological and cellular levels in the marine diatom Phaeodactylum tricornutum. Different biomarkers and stress responses in P. tricornutum were analysed when organisms were exposed to environmentally relevant PS NPs concentrations between 0.1 and 50 mg L−1. Our results showed significant differences between controls and exposure microalgae, indicating toxicity. After 24 h, an increase in oxidative stress biomarkers, damage to the photosynthetic apparatus, DNA damage and depolarization of mitochondrial and cell membrane from 5 mg L−1 were observed. Further after 72 h the inhibition of population growth and chlorophyll content were observed. Examining effects the effects related to PS NPs size, the smallest (50 nm) induced greater effects at 24 h while bigger PS NPs (100 nm) at72 h. This bigger particles (100 nm) showed more stability (in size distribution and spherical form) in the different culture media assayed, when compared with the rest of particles used. Strong adsorption and/or internalization of PS NPs was confirmed through changes in cell complexity and cell size as well as the fluorescence of 100 nm fluoresbrite PS NPs after washing cell surface.

The use of an integrative molecular approach can actively improve the evaluation of environmental health status and impact of chemicals, providing the knowledge to develop sentinel tools that can be integrated in risk assessment studies, since gene and protein expressions represent the first response barriers to anthropogenic stress.This work aimed to determine the mechanisms of toxic action of a widely applied fungicide formulation (chlorothalonil), following a time series approach and using a soil model arthropod, Folsomia candida. To link effects at different levels of biological organization, data were collected on reproduction, gene expression and protein levels, in a time series during exposure to a natural soil.Results showed a mechanistic mode of action for chlorothalonil, affecting pathways of detoxification and excretion, immune response, cellular respiration, protein metabolism and oxidative stress defense, causing irregular cell signaling (JNK and NOD ½ pathways), DNA damage and abnormal cell proliferation, leading to impairment in developmental features such as molting cycle and reproduction. The omics datasets presented highly significant positive correlations between the gene expression levels at a certain time-point and the corresponding protein products 2–3 days later. The integrated omics in this study has provided useful insights into pesticide mechanisms of toxicity, evidencing the relevance of such analyses in toxicological studies, and highlighting the importance of considering a time-series when integrating these datasets.

The accumulation of 17 human pharmaceuticals (HPs) was investigated in the muscle of three fish species characteristic of the “Rio de la Plata Basin” with different feeding habits and of relevance for human consumption: Megaleporinus obtusidens, Salminus brasiliensis, and Prochilodus lineatus. Fish were sampled in fall and spring from 8 localities distributed along 500 Km of the Uruguay River. Atenolol and carbamazepine were the most frequently detected HPs (>50%), but at concentrations always below 1 μg/kg wet weight (w/w). Hydrochlorothiazide, metoprolol, venlafaxine, propranolol, codeine, and the carbamazepine metabolite, 2-hydroxycarbamazepine, were accumulated at higher levels showing maximum concentrations between 1 and 10 μg/kg (w/w), but infrequently (<50%). The other HPs were always below 1 μg/kg (w/w) and at frequencies lower than 50%. Distinctive accumulation patterns were observed among species at different trophic levels. However, biomagnification trends were not identified for any compound. The highest number and concentration of HPs were found in M. obtusidens (omnivorous), followed by P. lineatus (detritivorous), and lastly S. brasiliensis (piscivorous). The most recurrent HPs (i.e. carbamazepine and atenolol) were present in all species, but others exclusively in one. Geographical variations were only found for carbamazepine and atenolol in M. obtusidens and P. lineatus, showing higher concentrations in localities closer to the Rio de la Plata estuary. Differences in the HPs concentrations among seasons were not identified. Acceptable daily intake and predicted no effect concentrations would indicate that measured muscle concentrations in fish from the Uruguay River do not pose a serious risk for human consumption nowadays. Further studies will be necessary for assessing the potential adverse effects on studied fish species.

Antidepressant drugs such as Venlafaxine (VFX) and O-desmethylvenlafaxine (ODMVFX) are emerging contaminants that are commonly detected in aquatic environments, since conventional wastewater treatment plants are unable to completely remove them. They can be precursors of hazardous by-products, such as the carcinogenic N-nitrosodimethylamine (NDMA), generated upon water chlorination, as they contain the dimethylamino moiety, necessary for the formation of NDMA. In this study, the capability of three white rot fungi (Trametes versicolor, Ganoderma lucidum and Pleurotus ostreatus) to remove both antidepressants from water and to decrease NDMA formation potential was investigated. Furthermore, transformation by-products (TPs) generated along the treatment process were elucidated and also correlated with their NDMA formation potential.Very promising results were obtained for T. versicolor and G. lucidum, both being able to remove up to 100% of ODMVFX. In the case of VFX, which is very recalcitrant to conventional wastewater treatment, a 70% of removal was achieved by T. versicolor, along with a reduction in NDMA formation potential, thus decreasing the associated problems for human health and the environment. However, the NDMA formation potential remained practically constant during treatment with G. lucidum despite of the equally high VFX removal (70%). This difference was attributed to the generation of different TPs during both fungal treatments. For example, G. lucidum generated more ODMVFX, which actually has a higher NDMA formation potential than the parent compound itself.

MicroRNAs (miRNAs) are a class of small non-coding RNA that control multiple biological processes through negative post-transcriptional regulation of gene expression. Recently a role of miRNAs in the response of aquatic organisms to environmental toxicants emerged. Toxicant-induced changes in miRNA expression might then represent novel biomarkers to evaluate the health status of these organisms. In this study, we aimed to identify the miRNA repertoire in the liver of the European eel Anguilla anguilla and to compare their differential expression between a polluted site located in the Gironde Estuary and a pristine site in Arcachon Bay (France).A total of 299 mature miRNAs were identified. In polluted water, 19 miRNAs were up-regulated and 22 were down-regulated. We predicted that these differentially expressed miRNAs could target 490 genes that were involved in ribosome biogenesis, response to hormones, response to chemical and chromatin modification. Moreover, we observed only few examples (29) of negative correlation between the expression levels of miRNAs and their targets suggesting that, in the system studied, miRNAs might not only regulate gene expression directly by degrading mRNA but also by inhibiting protein translation or by regulating other epigenetic processes.This study is the first example of in situ investigation of the role of miRNAs in the response of a fish species to water quality. Our findings provide new insights into the involvement of epigenetic mechanisms in the response of animals chronically exposed to pollution and pave the way for the utilization of miRNAs in aquatic ecotoxicology.