The combined effect of polystyrene microplastics (mPS) and dibutyl phthalate (DBP), a common plastic additive, on the microalgae Chlorella pyrenoidosa was investigated in the present study. The 96 h-IC₅₀ value of DBP was 2.41 mg L⁻¹. Polystyrene microplastics exhibited size-dependent inhibitory effect to C. pyrenoidosa, with the 96 h-IC₅₀ at 6.90 and 7.19 mg L⁻¹ for 0.1 and 0.55 μm mPS respectively, but little toxicity was observed for 5 μm mPS. The interaction parameter ρ based on the response additive response surface (RARS) model varied from −0.309 to 5.845, indicating the interaction pattern varying with exposure concentrations of chemical mixtures. A modified RARS model (taking ρ as a function of exposure concentration) was constructed and could well predict the combined toxicity of mPS and DBP. More than 20% reduction of DBP was observed at 20 mg L⁻¹ mPS, while 1 mg L⁻¹ mPS had no significant effect on the bioavailability of DBP at different sampling time points. Volume, morphological complexity and chlorophyll fluorescence intensity of microalgal cells were disturbed by both DBP and mPS. The antagonistic effect of high concentrations of mPS might be partially attributed to the combination of hetero- and homo-aggregation and the reduced bioavailability of DBP. The overall findings of the present study profiled the combined toxic effects of mPS and DBP on marine phytoplankton species which will be helpful for further evaluation of ecological risks of mPS and DBP in marine environment.

Multiple stressors like metal toxicity, organic compounds and sediment pollution from the Seine estuary are raising concern and novel toxicological approaches are needed to better assess and monitor the risk. In the present study, the copepod Eurytemora affinis from the Seine, was exposed to two different sources of contaminants, which were resuspended polluted sediments and a mixture of trace metals (dissolved phase). The exposure continued for four generations (F0, F1, F2, F3) where F0 is a generation for acclimation to the exposure condition and F3 is a generation for decontamination followed without any exposure, to detect possible maternal carryover effects of pollutants (F0 – F2) and the role of recovery (in F3). Higher accumulation of metals resulted in higher mortalities at both exposure conditions, with particularly F1 being the most sensitive generation showing highest bioaccumulation of metals, highest mortality, and smallest population size. Copper accumulation was highest of all metals in mixture from both the resuspended sediment and the combined trace metal treatment. A significantly lower naupliar production was seen in copepods exposed to resuspended sediment compared to trace metal exposed copepods. However, the decontamination phase (F3) indicated that E. affinis pre-exposed to resuspended sediment had a higher ability to recover the total population size, increase naupliar production, and depurate accumulated Cu. The population exposed to a trace metal mixture showed lower recovery and lower ability to discharge accumulated toxic metals indicating its greater effect on our experimental model when compared to resuspended sediment.

Microplastics are a complex group of ubiquitous environmental contaminants of emerging concern. These particles degrade slowly, release plasticizers, and can be transferred between trophic levels. In aquatic systems, they have been identified suspended in the water column, along shorelines, and within sediment. However, the abundance and distribution of microplastics in vegetated wetlands, which are transitional ecosystems between terrestrial and aquatic environments, are poorly understood. Here we describe the spatial distribution of soil microplastics in habitats of varying vegetation density in an urban tidal wetland. Samples were wet-sieved, organic matter was oxidized using hydrogen peroxide, and microplastics separated under a dissecting microscope, counted, and weighed. A fraction (n = 175) were analyzed via FTIR for validation. Positive microplastics identification was 81%–93%. Dominant polymers were polystyrene (29%) and polyethylene and synthetic rubber (both 8%). Average microplastic number to a 5-cm depth (23,200 ± 2,500 m−2 or 1,270 ± 150 kg−1) varied between habitat types, where mudflat, channel edge, and drift line habitats all had significantly more total microplastics than the interior of dense stands of vegetation, suggesting that emergent wetland plants are a highly effective filter of microplastics. Microfibers were about eight times as abundant as microfragments, and fibers and fragments differed in their distribution patterns, with microfibers most abundant in vegetation-free mudflats and microfragments in vegetated channel edges. Our results demonstrate that vegetated wetlands are important locations for microplastic accumulation and that wetland vegetation and hydrodynamics affect spatial distribution of microplastics between habitats.

Groundwater arsenic pollution causes millions of deaths worldwide. Long term natural and anthropogenic activities have increased arsenic levels in groundwater causing higher threats of arsenic exposure. Arsenic hyper-tolerant Firmicute Bacillus firmus L-148 was isolated from arsenic limiting Lonar lake soil, which tolerated more than 3 M arsenic and could oxidize 75 mM arsenite [As(III)] in 14 days. It oxidized As(III) in presence of heavy metals and had unusual pH optima at 9.2. B. firmus L-148 was studied at the biochemical, protein, genomic and transcript level for understanding its arsenic oxidizing machinery. The proteomic and transcript analysis exhibited the presence of ars and aio operon and supported the inducible nature of ars operon. Robust, hyper-tolerant, fast As(III) oxidizing, least nutrient requiring and multi-metal resistance qualities of the strain were used in microcosm studies for bioremediation. Artificial groundwater mimicking microcosm with 75 mM As(III) was developed. Modulation of carbon source, iron and multi metals affected growth and As(III) oxidation rate. The As(III) oxidation was recorded to be 77% in 15 days in presence of sodium acetate and Fe ions. This microcosm study can be explored for bioremediation of arsenic contaminated water and followed by precipitation using other methods.

Perfluorooctanoic acid (PFOA) has applications in numerous industrial products and is an industrial waste that is persistently present in the environment. Exposure to PFOA results in nonalcoholic fatty liver disease (NAFLD). However, the underlying mechanisms remain unclear. In this study, male C57BL/6 mice were exposed to PFOA (1 mg/kg/day) for 4 weeks to evaluate the effect of PFOA, and the human liver cell line (L-02) was used to observe the direct effect of PFOA in vitro. After PFOA exposure, the expression of genes related to hepatic lipogenesis, the NLRP3 inflammasome, and autophagy were measured. We found that exposure to PFOA induced lipid accumulation and stimulated lipogenesis in both mouse livers and L-02 cells. In addition, increased NLRP3 aggregation and enhanced production of IL-1β occurred after PFOA treatment. We also found that PFOA exposure induced autophagosome formation and p62 accumulation, indicating blockage of autophagic flux. Rapamycin alleviated PFOA-induced lipid accumulation and NLRP3 inflammasome activation by activating autophagic flux. Conversely, chloroquine, an autophagic flux inhibitor, exacerbated PFOA-induced lipid accumulation and NLRP3 inflammasome activation. Collectively, these results provide evidence to show that PFOA-induced blockade of autophagic flux causes an increase in lipid synthesis and inflammation in vivo and in vitro.

The widespread detection of 17β-estradiol (E2) in the environment has become an emerging concern worldwide due to its endocrine disrupting effects. This work focuses on the aerobic and anaerobic biodegradations of E2 in various sedimentary environments with different availabilities of electron acceptors, including O₂, NO₃⁻, Fe³⁺, SO₄²⁻, or HCO₃⁻. The highest removal efficiency (98.9%) and shortest degradation half-life of E2 (t₁/₂ = 5.0 d) were achieved under aerobic condition, followed by nitrate-reducing, ferric-reducing, sulfate-reducing and methanogenic conditions. We propose four different degradation pathways of E2 based on the metabolites identified under various redox conditions. Although most of E2 was effectively removed under aerobic condition, the potential environmental risk still needs to be considered due to the residual estrogenic activity induced by estrone (E1) formation. The endocrine-disrupting activities, as indicated by estradiol equivalent (EEQ) values, were related to E2 degradation rate and metabolite formation. We further analyzed the succession of bacterial community compositions and functions using Illumina HiSeq sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). The findings herein evidenced that bacterial community compositions and metabolic functions associated with different redox conditions impact the biodegradation of E2 and its endocrine-disrupting activity. This knowledge will be useful in predicting the environmental fates of estrogenic hormones in various sedimentary environments and aid in establishing appropriate strategies for eliminating potential environmental risks.

Rainwater chemistry plays an important role in the earth-surficial ecosystem, but studies on rainwater chemical composition of karst agro-ecosystem are rare. To explore the rainwater alkalization and the provenance of components responsible for neutralization, two-years chemical monitoring of rainwater was carried out in a karst agricultural catchment in Southwest China. The main findings suggest that SO₄²⁻, NO₃⁻, Ca²⁺, and NH₄⁺ are the principal ions. All the ionic contents show distinctly seasonal variation (highest in winter) in response to variations in seasonal precipitation because the rain-scour process can efficiently remove atmospheric materials. Source identification indicates that Cl⁻ and Na⁺ are mainly derived from marine input whereas SO₄²⁻ and NO₃⁻ are controlled by anthropogenic emission, in particular, fixed emission sources. The source of NH₄⁺ is attributed to intense agricultural production, while Ca²⁺ and Mg²⁺ are mainly derived from calcite dissolution. The rainwater alkalization caused by the seasonal acid neutralization (via basic components, Ca²⁺ and NH₄⁺) is beneficial to crop growth but also reflect agricultural overfertilization. Sulfur controlled the total wet acid deposition (68%–94%) and could be a potential agent of weathering.

Metals sequestered in coastal sediments are normally considered to be stable, but this investigation shows – somewhat surprisingly – that mercury concentrations in a previously contaminated area, Harboøre Tange, Denmark, have decreased since the 1980s. Mercury concentrations were determined in sediment and benthic biota and present values were compared to values in the 1980s and values from areas without known; history of mercury contamination. Concentrations in both the upper 20 cm of the sediments and; biota are considerably lower now compared to latest monitoring (1980s). Sediment.concentrations at most locations have decreased from the 100–300 ng Hg g⁻¹ dry weight (dw) level to levels below the Background Concentration (BC) of 50 ng Hg g⁻¹ dw defined by Oslo-Paris Convention for the Protection of the Marine Environment of the North-East Atlantic; some stations are at the 2–10 ng Hg g⁻¹ dw level characteristic of Danish coastal sediments with no known history of mercury contamination. Concentrations of mercury in the benthic biota along Harboøre Tange have also decreased since the 1980s but despite the lowered mercury concentrations in the sediments, concentrations in most samples of benthic invertebrate fauna still exceed those in uncontaminated coastal areas and also the Environmental Quality Standard (EQS) of 20 ng Hg g⁻¹ wet weight (≈100 ng Hg g⁻¹ dry weight) defined by the European Union’s Water Framework Directive. Concentration ranges in selected organisms are: (Harboøre Tange l980s/Harboøre Tange now/uncontaminated areas - given in ng Hg g⁻¹ dw): Periwinkles Littorina littorea 9000/150–450/55-77, blue mussels Mytilus edulis up to 9000/300–500/40–170, cockles Cerastoderma edule up to 8000/400–1200/200, brown shrimp Crangon crangon 700–2200/150-450/47, eelgrass Zostera marina up to 330/25–70/12. The present results - together with a literature review - show that a simple and straight forward relationship between the concentrations of mercury in sediment and benthic organisms does not necessarily exist.

This study reports comprehensive analysis of seasonal and inter-annual variations of aerosol properties (optical, physical and chemical) and radiative effects over Pokhara Valley in the foothills of central Himalayas in Nepal utilizing the high-quality multi-year columnar aerosol data observed recently from January 2010 to December 2017. This paper focusses on the seasonal and inter-annual variations of chemical (composition), and absorption properties of aerosols and their radiative effects. The single scattering albedo (SSA) either decreases as a function of wavelength or remains independent of wavelength. The seasonal mean aerosol absorption optical depth (AAOD) exhibits a behavior opposite to that of SSA. Carbonaceous aerosols (CA) dominate (≥60%) aerosol absorption during the whole year. Black carbon (BC) alone contributes >60% to AAODCA while brown carbon (BrC) shares the rest. The absorbing aerosol types are determined to be BC, and mixed (BC and dust) only. Dust as absorbing aerosol type is absent over the Himalayan foothills. The ARFSFC is ≥ -50 Wm⁻² except in monsoon almost every year. The ARFATM is ≥ 50 Wm⁻² during winter and pre-monsoon in all the years. ARFESFC, ARFETOA and ARFEATM follow a similar pattern as that of ARF. High values of ARFE at SFC, TOA and ATM (except during monsoon when values are slightly lower) suggest that aerosols are efficient in significantly modulating the incoming solar flux throughout the year. The annual average aerosol-induced atmospheric heating rate (HR) over Pokhara is nearly 1 K day⁻¹ every year during 8-year observation, and is highest in 2015 (∼2.5 K day⁻¹). The HR is about 1 K day⁻¹ or more over all the locations in IGP during the year. These quantitative results can be used as inputs in global/regional climate models to assess the climate impact of aerosols, including on regional temperature, hydrological cycle and melting of glaciers and snowfields in the region.

The current global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a tremendous public health challenge globally. While the respiratory transmission of SARS-CoV-2 has been established, evolving reports on the impact of the gastrointestinal system and the prolonged faecal shedding of SARS-CoV-2 show the likelihood of faecally mediated transmission. The increasing evidential presence of SARS-CoV-2 in wastewater and faecal material poses a significant public health threat which may potentiate global vulnerability to high risk of human exposure through environmental drivers especially in less developed countries. While extensively exploring the likelihood of faecally mediated SARS-CoV-2 transmission, infection control and prevention measures aimed at mitigating this pandemic should holistically include environmental drivers.