The study of aerosol dispersion characteristics in wastewater treatment plants (WWTPs) has attracted extensive attention. Oxidation ditch (OD) is a commonly implemented process during biological wastewater treatment. This study assessed the component characteristics, source apportionment, and exposure risks of aerosols generated from a WWTP using the OD process (AWO). The results indicated that the aeration part of oxidation ditch (ODA) exhibited the highest concentrations and proportions of the respiratory fractions (RF) of bacteria, Enterobacteriaceae, Staphylococcus aureus, and Pseudomonas aeruginosa. Some pathogenic or opportunistic-pathogenic bacteria and carcinogenic metal(loid)s were detected in the AWO. The source apportionment results indicated that the outdoor wastewater treatment processes and ambient air contributed to the constitution of the AWO. The indoor aerosols were mainly constituted by composition of the wastewater treatment process such as the sludge dewatering room (SDR). The pathogenic or opportunistic-pathogenic bacteria with eight genera (Colinsella, Dermatophilus, Enterobactor, Erycherichia-Shigella, Ledionella, Selenomonas, Xanthobacter, and Veillonella) were largely attributed to wastewater or sludge. The risk assessment suggested that inhalation was the main exposure pathway for aerosols (including bacteria and metal(loid)s). Additionally, As indicated the highest non-carcinogenic risks. Furthermore, As, Cd, and Co were associated with high carcinogenic risks. The ODA and sludge dewatering room (SDR) indicated the highest carcinogenic and non-carcinogenic risks of metal(loid)s, respectively. Thus, the AWO should be sufficiently researched and monitored to mitigate their harmful effects on human health, particularly with regard to the health of the site workers.

The persistence and toxicity of second generation anticoagulant rodenticides (SGARs) in animal tissues make these compounds dangerous by biomagnification in predatory species. Here we studied the levels of SGARs in non-target species of wildlife and the environmental factors that influence such exposure. Liver samples of terrestrial vertebrates (n = 244) found dead between 2007 and 2016 in the region of Aragón (NE Spain) were analysed. The presence of SGARs was statistically analysed with binary or ordinal logistic models to study the effect of habitat characteristics including human population density, percentage of urban surface, livestock densities and surface of different types of crops. SGARs residues were detected in 83 (34%) of the animals and levels >200 ng/g were found in common raven (67%), red fox (50%), red kite (38%), Eurasian eagle-owl (25%), stone marten (23%), Eurasian buzzard (17%), northern marsh harrier (17%), and Eurasian badger (14%). The spatial analysis revealed that the presence of SGARs residues in wildlife was more associated with the use of these products as biocides in urban areas and cattle farms rather than as plant protection products in agricultural fields. This information permits to identify potential habitats where SGARs may pose a risk for predatory birds and mammals.

Antibiotic resistance (AR) in the environment is a growing and global concern for public health, and intrinsic AR from pristine sites untouched by pharmaceutical antibiotics is not commonly studied. Forty aerobic bacteria were isolated from water and sediment samples of hot springs in South Africa. Resistance against ten antibiotics (carbenicillin, gentamicin, kanamycin, streptomycin, tetracycline, chloramphenicol, ceftriaxone, co-trimoxazole, nalidixic acid and norfloxacin) was tested using a standard disk diffusion assay. Resistance to one or two antibiotics were equally found in 37.5%, while the remaining 22% showed complete sensitivity. Intermediate resistance was found for ceftriaxone (52.5%), nalidixic acid (37.5%) and carbenicillin (22.5%), while low levels of resistance were observed for streptomycin (5%) and kanamycin (2.5%), and total sensitivity towards the other antibiotics. Twenty-nine isolates were also tested against eight different heavy-metal salts (Al, Cr, Cu, Fe, Hg, Mn, Ni and Pb) at 10 and 40 mM. All isolates were tolerant and able to grow on ≥2 heavy-metal salts at both concentrations. No association was observed between AR and heavy metal tolerance (HMT). Based on the relatively low AR levels, hot spring sites are pristine environments reflecting baseline levels for comparison to other potentially contaminated groundwater sites.

The fast-growing production and application of carbon nanotube (CNT) materials in a variety of industrial products inevitably lead to their release to wastewater and surface water. CNT would experience oxidization in wastewater treatment plant due to the presence of large amount of disinfectants, such as H₂O₂ and O₃, which in turn affects the environmental fates and risks of CNT. In this study, oxidized CNT materials (O-CNTs) were prepared by treating CNT with H₂O₂/UV and O₃ (denoting as H₂O₂-CNT and O₃-CNT, respectively). A variety of characterizations indicated that oxygen containing groups were generated on CNT surface upon the oxidation, and the O/C ratio increased in the order of pristine CNT < H₂O₂-CNT < O₃-CNT. In the presence of Na⁺, K⁺ and Mg²⁺, the O-CNTs displayed better colloidal stability than the pristine CNT, and the stability increased with the oxidation degree (indicated by O/C ratio). This could be explained by the more negative surface charge and stronger hydrophilicity of the O-CNTs. Unexpectedly, in the presence of Ca²⁺, the most oxidized O₃-CNT exhibited the poorest colloidal stability. The abundant carboxyl groups in O₃-CNT provided effective binding sites for cation bridging effect through Ca²⁺ and led to stronger aggregation. Increasing pH was more favorable to disperse CNTs (both O-CNT and pristine CNT) in the presence of Na⁺, but much less effective in inhibiting the aggregation of O₃-CNT in presence of Ca²⁺. This could be explained by the stronger cation bridging effect due to enhanced deprotonation the –COOH groups at higher pH conditions. The calculated Hamaker constants of the CNTs decreased with the oxidation degree, implying that there was lower van der Waals force between the O-CNTs. The Derjaguin–Landau–Verwey–Overbeek (DLVO) calculation confirmed that O-CNTs had to overcome higher energy barrier and thus showed better colloidal stability than the pristine CNT in the presence of Na⁺.

The transport and retention of nanoplastics (NP, 200 nm nanopolystyrene) functionalized with surface carboxyl (NPC), sulfonic (NPS), low-density amino (negatively charged, NPA−), and high-density amino (positively charged, NPA+) groups in seawater-saturated sand with/without humic acid were examined to explore the role of NP surface functionalities. The mass percentages of NP recovered from the effluent (Meff) with a salinity of 35 practical salinity units (PSU) were ranked as follows: NPC (19.69%) > NPS (16.37%) > NPA+ (13.33%) > NPA− (9.78%). The homoaggregation of NPS and NPA− was observed in seawater. The transport of NPA− exhibited a ripening phenomenon (i.e., a decrease in the transport rate with time) due to the high attraction of NP with previously deposited NP, whereas monodispersed NPA+ presented a low Meff value because of the electrostatic attraction between NPA+ and negatively charged sand. Retention experiments showed that the majority of NPC, NPS and NPA+ accumulated in a monolayer on the sand surface, whereas NPA− accumulated in multiple layers. Suwannee River humic acid (SRHA) could remarkably improve the transportability of NPC, NPS, and NPA− by increasing steric repulsion. The strong attraction between NPA+ and the deposited NPA+ in the presence of SRHA triggered the weak ripening phenomenon. As seawater salinity decreased from 35 PSU to 3.5 PSU, the increase in electrostatic repulsion of NP-NP and NP-sand enhanced the transport of NPC, NPS, and NPA−, and the ripening of NPA− breakthrough curves disappeared. In deionized water, NPC, NPS, and NPA− achieved complete column breakthrough because the electrostatic repulsion between NP and sand intensified. However, the Meff values of NPA+ in 3.5 PSU seawater and deionized water presented limited increments of 15.49% and 23.67%, respectively. These results indicated that the fate of NP in sandy marine environments were strongly affected by NP surface functionalities, seawater salinity, and coexisting SRHA.

The anthropogenic marine debris, especially abandoned, lost or otherwise discarded Fishing Gear (ALDFG), represents a rising concern, because of its potential harmful impact on the marine animal forests. We carried out 13 km of video recordings, by means of a remotely operated vehicle, from 10 to 210 m depth, in an anthropised area of the Tyrrhenian Sea (Mediterranean Sea). This site, for its high ecological importance and biodiversity value, has been identified for the establishment of a new marine protected area (MPA). The aim of this paper was to assess marine litter abundance and its effects on the benthic fauna. The debris density, in the study area, ranged from 0.24 to 8.01 items/100 m2, with an average of 3.49 (±0.59) items/100 m2. The derelict fishing gear, mainly fishing lines, were the main source of marine debris, contributing 77.9% to the overall litter. The impacts of debris on the benthic fauna were frequently recorded, with 28.5% of the litter entangling corals and impacting habitats of conservation concern. These impacts were exclusively caused by the derelict fishing gear (91.2% by longlines), and the highest percentage (49.1%) of ALDFG causing impacts was observed from 41 to 80 m depth, in the coralligenous biocenosis. The results of the present study will help the fulfilment of “harm” monitoring, as recommended by the Marine Strategy Framework Directive (MSFD) and the UN Environment/MAP Regional Plan on the marine litter management in the Mediterranean Sea. Regarding the actions to reduce the derelict fishing gear, preventive measures are usually preferred instead of the extensive removals based on cost-effectiveness and sustainability. The establishment of a new MPA in the area could be a good solution to reduce ALDFG, resulting in the improvement of the ecological status of this coastal area.

Currently, there is little comparative data on the colloidal stability and the toxicity of ultraviolet (UV)- and visible-light (VL)-transformed graphene oxide (GO). In order to identify this knowledge gap, the physicochemical properties of UV/VL-transformed GO are investigated in detail. Attempts are made to correlate the physicochemical alterations of UV/VL-transformed GO to the observed changes in its colloidal properties and toxicity. The results show that both UV and VL irradiations induce the significant change in the color, UV–vis absorbance, morphology, surface charge, size, oxygen containing functional groups, total of carbon, and photoluminescence properties of GO. The photo-reaction behavior of GO under UV exposure is different from that under VL irradiation in terms of reaction rate, order, and extent. Finally, the UV and VL irradiations show different effects not only on the colloidal stability of GO in the City water and Dongpu Lake water, but also on the toxicity of GO to Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria. This study clearly shows how the environmental fate and risk of GO are modified by UV and VL irradiations.

While various bioremediation techniques have been widely used at oil spill sites, the in situ efficiency of such techniques on recovering the benthic communities in intertidal areas has not been quantified. Here, the performance of several bioremediation tools such as emulsifiers, multi-enzyme liquid (MEL), microbes, and rice-straw was evaluated by a 90-days semi-field experiment, particularly targeting recovery of benthic community. Temporal efficiency in the removal of sedimentary total petroleum hydrocarbons (TPH), reduction of residual toxicity, and recovery of bacterial diversity, microalgal growth, and benthic production was comprehensively determined. Concentrations of TPH and amphipod mortality for all treatments rapidly decreased within the first 10 days. In addition, the density of bacteria and microphytobenthos generally increased over time for all treatments, indicating recovery in the benthic community health. However, the recovery of some nitrifying bacteria, such as the class Nitrospinia (which are sensitive to oil components) remained incomplete (13–56%) during 90 days. Combination of microbe treatments showed rapid and effective for recovering the benthic community, but after 90 days, all treatments showed high recovery efficiency. Of consideration, the “no action” treatment showed a similar level of recovery to those of microbe and MEL treatments, indicating that the natural recovery process could prevail in certain situations.

A severe air quality degradation event occurred in the Santiago Metropolitan Area (SMA), Chile, in June 2014. Meteorological and air quality measurements from 11 stations in the area as well as numerical simulations using the Weather and Research Forecasting (WRF) model were used to explain the main reasons for the occurrence of elevated particulate matter (PM) concentrations. The conditions were characterized with formation of a coastal low in central Chile between the southeastern anticyclone and a high-pressure system over Argentina. At a local scale, these conditions generated a depression at the base of the inversion layer, an increase in the vertical thermal stability, lower humidity and low-wind conditions, which were conducive to a decrease in pollutant dispersion and insufficient ventilation of the polluted air. Measurements and simulations using the WRF model revealed a vertical structure of the boundary layer during these stagnant conditions and provided a basis for a trajectory analysis. The back-trajectory calculation showed that the transport of air parcels was contained in the valley during the highest concentrations. The analysis also enabled the definition of the threshold values of a simple indicator of air pollution (ventilation coefficient, VC), which confirmed the evolution of the episode and divided the observed daily concentrations into two groups, with one including values above the limits prescribed by the national air quality standards (NAQS) and the other including values below these limits. For the SMA, the daily PM concentrations above the NASQ limits were associated with an overall mean threshold value of VC below 500 m² s⁻¹ (for PM₂.₅) and 300 m² s⁻¹ (for PM₁₀). To apply the VC analysis to other pollutants and different geographic locations, different threshold values should be evaluated.

Pharmaceuticals and analogs of bisphenol A (BPA) are increasingly threatening environmental pollutants. In this study, mixtures of selected pharmaceuticals (diclofenac sodium salt, chloramphenicol, oxytetracycline hydrochloride, fluoxetine hydrochloride, estrone, ketoprofen, progesterone, gemfibrozil and androstenedione) were prepared with BPA and its two analogs (namely, bisphenols F and S) at such ratios to reflect environmentally detectable levels. Then, the mixture solutions were studied with a XenoScreen YES/YAS assay to determine the variations in the initial hormonal response of each pharmaceutical compound due to the presence of a bisphenol analog. The results obtained were modeled with the concentration addition (CA) and independent action (IA) approaches, the trueness of which was studied with model deviation ratios (MDR). The estrogenic agonistic activity of the drugs studied was most strongly affected by the presence of BPA in solution (twenty-one cases of synergy observed for CA models versus twelve cases of antagonism in the case of IA predictions). BPS shows a strong agonistic estrogenic impact on most of the drugs studied at medium and high concentration levels; androgenic agonistic activity was also impaired with elevated concentrations of BPS. Increasing the concentration of BPF in a reaction mixture also increased the number of YES + synergism incidences (for CA modeling). Estrone, progesterone and androstenedione were mostly affected by the highest BPF concentrations studied in the case of androgenic agonistic research performed.