Southeast Asia is particularly susceptible to the negative impacts of increasing coastal pollution as coastal populations and cities grow at unprecedented rates. Although water chemistry can be monitored, there are greater advantages in using bioindicators as reflectors of the combined effect of multiple pollution types on coastal ecosystem health and for early detection of the negative impacts of pollutants on biotic systems. This study explores the utility and application of ostracods as pollution bioindicators and examines the response of ostracod assemblages to variable pollution in Lap An Lagoon, central Vietnam. From 14 sites within the lagoon, 79 species of 46 genera were identified and sediment grain size, total organic carbon, organic matter and heavy metal concentration were measured. Cluster analysis, detrended correspondence analysis and canonical correspondence analysis identified four distinct ostracod biofacies that were highly correlated to the physical environmental variables (salinity, depth, sediment type, heavy metal concentrations, total organic carbon and organic matter) and are shown to be the main factors controlling ostracod biofacies. Low ostracod diversities were found in silty sediments with heavy metal concentrations likely toxic. Sinocytheridea impressa was indicative of a marginally polluted environment within the lagoon. This study provides evidence for the potential for Southeast Asian ostracods to be used in water quality assessments and the data collected can be used as a baseline for future pollution monitoring.

Quantitative estimation of soil organic carbon (SOC) is essential for the study of the C cycle and global C storage. Soil spectroscopic technology provides a cost-effective and time-efficient method for SOC quantification and has been successfully used to determine SOC storage. However, the SOC estimation accuracy remains limited by other soil properties, particularly soil water. In this study, we proposed a new deep learning algorithm named the Water Absorption Trough Dewatering Machine (WATDM) to improve estimations of SOC from soil reflectance spectra and reduce the effect of soil water. Soil water and reflectance spectral data of soil samples were measured using spectrometry. Based on the soil water contents derived from the water absorption troughs around 1900 nm, the optimal WATDM model was obtained and treated as the final model of the WATDM method, which performed better than a multiple linear regression model based on moist soil samples. The findings of this study indicate that the WATDM method can improve the estimation accuracy of SOC content by reducing the effect of soil water and can be used as a valuable new methodology within the spectroscopic estimation of soil properties.

Emerging evidence has demonstrated that exposure to fine particulate matter (PM₂.₅) is a risk factor for lipid metabolic disorders in the liver. However, the effects of PM₂.₅ exposure time duration on hepatic lipid metabolism remain unknown. In this study, C57BL/6 mice were randomly divided into ambient PM₂.₅ (PM) or filtered air (FA) exposure chamber for short-term (4 weeks) or long-term (24 weeks) exposure via a whole body exposure system. We measured hepatic triglyceride and free fatty acid levels and analyzed the alteration of lipometabolism-related molecules in the liver. We found that triglyceride levels were significantly elevated in both short-term and long-term PM₂.₅-exposed mice and free fatty acid levels were increased after long-term PM₂.₅ exposure. Besides, enzymes for lipolysis and fatty acid oxidation in the liver were inhibited after short-term PM₂.₅ exposure but adaptively enhanced after long-term PM₂.₅ exposure. Furthermore, molecules for fatty acid uptake were down-regulated in the short-term PM₂.₅-exposed mice whereas molecules for lipid export were induced after long-term PM₂.₅ exposure. Therefore, ambient PM₂.₅ exposure disturbed hepatic lipid metabolism and the effects varied in different exposure duration. These findings in mice provide new insight into the biological basis of PM₂.₅-induced human metabolic dysfunction and specific strategies may be applied based on different exposure time periods.

Infection caused by pathogenic bacteria carrying antibiotic resistance genes (ARGs) is a serious challenge to human health. Water environment, including water and surface sediments, is an important repository of ARGs, and the activity of aquatic animal can affect the development of ARG pollution in the water environment. Macrobenthic invertebrates are an important component of aquatic ecosystems, and their effects on ARG development in aquatic environments remain unreported. The distribution of ARGs, including tetA gene, sul2 gene, and kan gene, in Chironomidae larvae is demonstrated in this study for the first time. The ARG distribution was related to sampling points, metal elements, and seasons. Animal models demonstrated that Chironomidae larvae enriched ARGs from water and passed them on to downstream predators in the food chain. Conjugative transfer mediated by resistant plasmids was crucial in the spread of ARG in Chironomidae larvae, and upregulated expression of trfAp gene and trbBp gene was the molecular mechanism. Escherichia in Proteobacteria and Flavobacterium in Bacteroidetes, which are gram-negative bacteria in Chironomidae larvae, are the primary host bacteria of ARGs confirmed via resistance screening and DNA sequencing of V4 region of 16S rRNA gene. Feeding experiments further confirmed that ARGs from Chironomidae larvae can be enriched in the fish gut. Research gaps in food chain between sediments and fish are addressed in this study, and Chironomidae larvae is an important enricher of ARGs in the freshwater environment.

Microplastics (MPs) as carriers of various contaminants have attracted more attentions in water environments. However, the interactions between typical MPs and norfloxacin (NOR) in natural water environments were still not systematically studied. In this study, the adsorption of NOR onto four typical types of MPs (polyethylene (PE), polypropylene (PP), polystyrene (PS), and polyvinyl chloride (PVC)) was investigated in simulated natural water and real surface water, and the adsorption mechanisms were deeply explored to provide fundamental understandings of the MPs-NOR complicated pollution. The results showed that the kinetics of NOR onto all MPs obeyed pseudo-second-order model, and was greatly slowed down at lower temperature or higher salinity. The intrinsic structure and surface area of MPs played important roles in the adsorption behaviors of NOR on these four types of MPs. The adsorption isotherm of NOR onto all MPs could be well described by linear model, with the Kd values following the order of PVC > PS > PE > PP (i.e. 6.229–11.901 L/μg) in simulated natural water. However, in surface water the adsorption isotherms of NOR on all MPs could be well fitted by Freundlich model. For all MPs, the adsorption of NOR was quite pH-dependent due to the electrostatic interactions. Furthermore, the salinity and the presence of dissolved organic matter (DOM) had significantly hindered the NOR adsorption. More importantly, compared with adsorption behaviors in simulated natural water, the competition of coexisting substances such as cations and NOM for adsorption sites and higher water pH dramatically reduced the adsorption of NOR onto all types of MPs in Jiang'an River, with the reduction rate of 19.7–41.2%. Finally, the mechanism studies indicated that the electrostatic attractions played a key role in the adsorption of NOR onto MPs, and π-π, H-bonding, polar-polar, and Van Der Waals interactions were also involved in adsorption processes.

While polychlorinated biphenyl (PCB)-related risks have been reported at the cellular, organ, and individual levels in some marine mammals, studies quantifying the PCB-associated population-level effects are limited. Here, we combined chemical analysis and individual-based model simulation to investigate the impact of PCBs on the Indo-Pacific humpback dolphin (sub)population from the Pearl River Estuary (PRE). An annual PCB accumulation rate of 0.29 ± 0.07 mg/kg lipid per year was estimated based on the measured age-specific male data as males continue to accumulate PCBs throughout their lifetime, without depurating contaminant loads. Using the Taiwan Strait dolphin population with low PCBs as a baseline, we compare our model simulations in PRE population to estimate relative population impacts of PCBs and other stressors. When using the current vital rates of the PRE dolphins which have been affected by PCBs and other stressors (e.g., underwater noise, prey limitation, etc.), our simulations revealed a substantial decline (8.1%) in the annual population growth rate (λ) of PRE metapopulation compared to baseline over the next 100 years. At the estimated PCB accumulation rate, the PCB-mediated effects on calf survival and immunity would cause a slight decline (0.9%) in λ relative to baseline. Our findings suggest a relatively limited impact of PCBs on the long-term survival of PRE dolphins among all stressors. However, it should be noted that even under model simulations where dietary PCBs were eliminated, humpback dolphins would still need a long time to reduce their PCB burdens to a relatively “safe” level through biological cycling. Considering that the baseline vital rates might also have been affected by PCBs and other stressors, our results are considered relative rather than absolute. This study provides a starting point for quantifying population-level consequences of contaminant exposure on humpback dolphins, although more efforts are needed to perfect this type of analysis.

The agroeconomic benefits of the routine use of triazole fungicides on crops have been evident for more than 40 years. However, increasing evidence shows that residues of triazoles are ubiquitous in various foods and thus could pose a potential health risk to humans. We analyzed 3406 samples of 13 food commodities that were collected from markets in 9 regions across China, and assessed the health risk of both chronic and acute exposure to the triazoles for Chinese children (1–6 years old) and the general population. Among all samples, 55.52% had triazoles in concentrations of 0.10–803.30 μg/kg, and 29.77% of samples contained a combination of 2–7 triazoles. Tebuconazole and difenoconazole were the most commonly found triazoles in the foods, being detected in 33.44% and 30.45% of samples, respectively. Chronic and acute cumulative risk assessment for total triazoles based on a relative potency factor method revealed that exposure to triazoles from these particular commodities was below the levels that might pose a health risk (chronic hazard index range, 5.90×10⁻⁷ to 1.83×10⁻³; acute hazard index range, 7.77×10⁻⁵ to 0.39, below 1). Notably, dietary exposure risk for children was greater than that for the general population—particularly for the acute intake of mandarin, grape, and cucumber (acute hazard index values of 0.35–0.39). Despite the low health risk, the potential hazards of exposure to triazoles should raise public concern owing to their ubiquitous presence in common foods and potential cumulative effects.

The benefits of the artificial fixation of reactive nitrogen (Nr, nitrogen [N] compounds other than dinitrogen), in the form of N fertilizers and materials are huge, while at the same time posing substantial threats to human and ecosystem health by the release of Nr to the environment. To achieve sustainable N use, Nr loss to the environment must be reduced. An N-budget approach at the national level would allow us to fully grasp the whole picture of Nr loss to the environment through the quantification of important N flows in the country. In this study, the N budgets in Japan were estimated from 2000 to 2015 using available statistics, datasets, and literature. The net N inflow to Japanese human sectors in 2010 was 6180 Gg N yr⁻¹ in total. With 420 Gg N yr⁻¹ accumulating in human settlements, 5760 Gg N yr⁻¹ was released from the human sector, of which 1960 Gg N yr⁻¹ was lost to the environment as Nr (64% to air and 36% to waters), and the remainder assumed as dinitrogen. Nr loss decreased in both atmospheric emissions and loss to terrestrial water over time. The distinct reduction in the atmospheric emissions of nitrogen oxides from transportation, at −4.3% yr⁻¹, was attributed to both emission controls and a decrease in energy consumption. Reductions in runoff and leaching from land as well as the discharge of treated water were found, at −1.0% yr⁻¹ for both. The aging of Japan's population coincided with the reductions in the per capita supply and consumption of food and energy. Future challenges for Japan lie in further reducing N waste and adapting its N flows in international trade to adopt more sustainable options considering the reduced demand due to the aging population.

PM₂.₅ (particulate matter ≤2.5 μm in aerodynamic diameter) is a major urban air pollutant worldwide. Its effects on the respiratory system of the susceptible population have been less characterized. This study aimed to estimate the association of short-term PM₂.₅ exposure with respiratory outcomes of the retired adults, and to examine whether these associations were stronger among the subjects with GSTT-null genotype. 32 healthy subjects (55–77 years) were recruited for five follow-up examinations. Ambient concentrations of PM₂.₅ were monitored consecutively for 7 days prior to physical examination. Pulmonary outcomes including forced vital capacity (FVC), forced expiratory volume in 1 s (FEV₁), peak expiratory flow (PEF), and fractional exhaled nitric oxide (FeNO), and nasal fluid concentrations of 8-epi-prostaglandin F2 alpha (8-epi-PGF2α), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8) and IL-1β were measured. A linear mixed-effect model was introduced to evaluate the associations of PM₂.₅ concentrations with respiratory outcomes. Additionally, GSTT1 genotype-based stratification was performed to characterize modification on PM₂.₅-related respiratory outcomes. We found that a 10 μg/m³ increase in PM₂.₅ was associated with decreases of 0.52 L (95% confidence interval [CI]: -1.04, -0.002), 0.64 L (95% CI: -1.13, -0.16), 0.1 (95% CI: -0.23, 0.04) and 2.87 L/s (95% CI: -5.09, -0.64) in FVC, FEV₁, FEV₁/FVC ratio and PEF at lag 2, respectively. Meanwhile, marked increases of 80.82% (95% CI: 5.13%, 156.50%) in IL-8, 77.14% (95% CI: 1.88%, 152.40%) in IL-1β and 67.87% (95% CI: 14.85%, 120.88%) in 8-epi-PGF2α were observed as PM₂.₅ concentration increased by 10 μg/m³ at lag 2. Notably, PM₂.₅-associated decreases in FVC and PEF and increase in FeNO were stronger among the subjects with GSTT1-null genotype. In summary, short-term exposure to PM₂.₅ is associated with nasal inflammation, oxidative stress and lung function reduction in the retired subjects. Lung function reduction and inflammation are stronger among the subjects with GSTT1-null genotype.

Three Gorges Dam (TGD) is the largest hydroelectric construction in the world, and its potential impacts on the ecological environment and human health risks have invoked considerable global concern. However, as a mercury (Hg) sensitive system, limited work was conducted on the Hg exposure level of local residents around the Three Gorges Reservoir (TGR). Thus, 540 human hair samples and 22 species of local food samples were collected to assess the Hg exposure and human health risk to the residents located in the Three Gorges Reservoir Region (TGRR) and to investigate their dietary exposure to Hg. The results showed that the geometric mean concentrations of total mercury (THg) and methylmercury (MeHg) in hair were 0.42 ± 0.43 μg g⁻¹ and 0.23 ± 0.32 μg g⁻¹, respectively, lower than the reference level (1.0 μg g⁻¹) recommended by the United States Environmental Protection Agency (US EPA), indicating a low level Hg exposure for residents around the TGR. No significant difference in the accumulation of Hg in hair between the gender subgroups was observed, whereas age difference, smoking and alcohol drinking behavior, and fish consumption frequency were significant predictors of hair Hg level. Besides, THg and MeHg of all the investigated food samples did not exceed the corresponding Chinese national standard. The average probable daily intakes (PDIs) of THg and MeHg were 0.032 μg kg⁻¹ day⁻¹ and 0.007 μg kg⁻¹ day⁻¹, which were obviously below the recommended values of 0.57 μg kg⁻¹ day⁻¹ and 0.1 μg kg⁻¹ day⁻¹, respectively. The cereal (mainly rice) contribution of THg (76.0%) and MeHg (74.4%) intakes to the local residents around the TGR was much higher than that of fish (10.7% and 22.9%, respectively) due to the considerable rice consumption. Overall, residents around the TGR were at a low Hg exposure and rice consumption was the major pathway for Hg exposure.