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Assessing applicability of the paper-disc method used in combination with flow cytometry to evaluate algal toxicity 全文
2018
Nam, Sun-Hwa | Kwak, Jin Il | An, Youn-Joo
Soil algal bioassays have been limited by their inability to evaluate several toxic endpoints because it is difficult to collect pure soil algae growing on and beneath the soil surface. This study describes the extension of a previously developed paper-disc method for analyzing soil toxicity to algae. The method can be used in conjunction with flow cytometric analysis and facilitates the assessment of previously proposed toxicity endpoints, such as the growth zone, biomass, and photosynthetic activity. We assessed the applicability of this paper-disc soil method using the green algae Chlamydomonas reinhardtii and Pseudokirchneriella subcapitata exposed to nickel-contaminated soil; examined cell sizes, cell granularity, enzyme activity, and oxidative stress as new toxicity endpoints using flow cytometry; and identified morphological changes in green algae assayed. The results showed that, used in conjunction with flow cytometry, the extended paper-disc soil method is sufficiently sensitive to detect decreases in cell granularity in C. reinhardtii and esterase activity in P. subcapitata. The method also revealed decreases in growth zone, biomass, and electron transfer from the reaction center to the quinone pool. Collectively, the results of this study indicate that soil algal bioassays using nonspecific algae can be used to assess soil quality, to derive several toxicity endpoints for individual cells, and to evaluate previously established flow cytometric toxicity endpoints.
显示更多 [+] 显示较少 [-]Residuals of organophosphate esters in foodstuffs and implication for human exposure 全文
2018
Ding, Jinjian | Deng, Tongqing | Xu, Mengmeng | Wang, Shen | Yang, Fangxing
Foodstuffs may be contaminated by organophosphate esters (OPEs) and become an important source of human exposure since OPEs are ubiquitous in the environment. In the present study, 10 OPEs were analyzed in various food matrices collected from a city in Eastern China including chicken, pork, fishes, vegetables, tofu, eggs, milk and cereals. The concentrations of Σ₁₀OPEs ranged from 1.1 to 9.6 ng g⁻¹ fresh weight (fw) in the foodstuffs. Cereals had the highest residual level of total OPEs with a mean value of 5.7 ng g⁻¹ fw. Tris(2-ethylhexyl) phosphate was detected in all foodstuff samples and showed the highest median residual concentration of 1.3 ng g⁻¹ fw among the OPE analogs. The daily dietary intake of OPEs was calculated as 3.6 and 2.4 μg d⁻¹ for adults and children. Cereals were identified as the major contributor to the total OPEs among different types of foodstuffs. Preliminary exposure assessment revealed that the current non-cancer health risks of OPEs via dietary intake were in the range of 10⁻⁵-10⁻³, indicating low risk levels. Moreover, the hazard index of OPEs indicated that the risk for children (3 × 10⁻³) was higher than adults (2 × 10⁻³).
显示更多 [+] 显示较少 [-]VOC emissions and carbon balance of two bioenergy plantations in response to nitrogen fertilization: A comparison of Miscanthus and Salix 全文
2018
Hu, Bin | Jarosch, Ann-Mareike | Gauder, Martin | Graeff-Hönninger, Simone | Schnitzler, Jörg-Peter | Grote, Rüdiger | Rennenberg, H. (Heinz) | Kreuzwieser, Jürgen
Energy crops are an important renewable source for energy production in future. To ensure high yields of crops, N fertilization is a common practice. However, knowledge on environmental impacts of bioenergy plantations, particularly in systems involving trees, and the effects of N fertilization is scarce. We studied the emission of volatile organic compounds (VOC), which negatively affect the environment by contributing to tropospheric ozone and aerosols formation, from Miscanthus and willow plantations. Particularly, we aimed at quantifying the effect of N fertilization on VOC emission. For this purpose, we determined plant traits, photosynthetic gas exchange and VOC emission rates of the two systems as affected by N fertilization (0 and 80 kg ha−1 yr−1). Additionally, we used a modelling approach to simulate (i) the annual VOC emission rates as well as (ii) the OH. reactivity resulting from individual VOC emitted. Total VOC emissions from Salix was 1.5- and 2.5-fold higher compared to Miscanthus in non-fertilized and fertilized plantations, respectively. Isoprene was the dominating VOC in Salix (80–130 μg g−1 DW h−1), whereas it was negligible in Miscanthus. We identified twenty-eight VOC compounds, which were released by Miscanthus with the green leaf volatile hexanal as well as dimethyl benzene, dihydrofuranone, phenol, and decanal as the dominant volatiles. The pattern of VOC released from this species clearly differed to the pattern emitted by Salix. OH. reactivity from VOC released by Salix was ca. 8-times higher than that of Miscanthus. N fertilization enhanced stand level VOC emissions, mainly by promoting the leaf area index and only marginally by enhancing the basal emission capacity of leaves. Considering the higher productivity of fertilized Miscanthus compared to Salix together with the considerably lower OH. reactivity per weight unit of biomass produced, qualified the C4-perennial grass Miscanthus as a superior source of future bioenergy production.
显示更多 [+] 显示较少 [-]Air pollution-derived PM2.5 impairs mitochondrial function in healthy and chronic obstructive pulmonary diseased human bronchial epithelial cells 全文
2018
Leclercq, B. | Kluza, J. | Antherieu, S. | Sotty, J. | Alleman, L.Y. | Perdrix, E. | Loyens, A. | Coddeville, P. | Lo Guidice, J.-M. | Marchetti, P. | Garçon, G.
In order to clarify whether the mitochondrial dysfunction is closely related to the cell homeostasis maintenance after particulate matter (PM₂.₅) exposure, oxidative, inflammatory, apoptotic and mitochondrial endpoints were carefully studied in human bronchial epithelial BEAS-2B, normal human bronchial epithelial (NHBE) and chronic obstructive pulmonary disease (COPD)-diseased human bronchial epithelial (DHBE) cells acutely or repeatedly exposed to air pollution-derived PM₂.₅. Some modifications of the mitochondrial morphology were observed within all these cell models repeatedly exposed to the highest dose of PM₂.₅. Dose- and exposure-dependent oxidative damages were reported in BEAS-2B, NHBE and particularly COPD-DHBE cells acutely or repeatedly exposed to PM₂.₅. Nuclear factor erythroid 2-p45 related factor 2 (NRF2) gene expression and binding activity, together with the mRNA levels of some NRF2 target genes, were directly related to the number of exposures for the lowest PM₂.₅ dose (i.e., 2 μg/cm²), but, surprisingly, inversely related to the number of exposures for the highest dose (i.e., 10 μg/cm²). There were dose- and exposure-dependent increases of both nuclear factor kappa-B (NF-κB) binding activity and NF-κB target cytokine secretion in BEAS-2B, NHBE and particularly COPD-DHBE cells exposed to PM₂.₅. Mitochondrial ROS production, membrane potential depolarization, oxidative phosphorylation, and ATP production were significantly altered in all the cell models repeatedly exposed to the highest dose of PM₂.₅. Collectively, our results indicate a cytosolic ROS overproduction, inducing oxidative damage and activating oxygen sensitive NRF2 and NF-ₖB signaling pathways for all the cell models acutely or repeatedly exposed to PM₂.₅. However, one of the important highlight of our findings is that the prolonged and repeated exposure in BEAS-2B, NHBE and in particular sensible COPD-DHBE cells further caused an oxidative boost able to partially inactivate the NRF2 signaling pathway and to critically impair mitochondrial redox homeostasis, thereby producing a persistent mitochondrial dysfunction and a lowering cell energy supply.
显示更多 [+] 显示较少 [-]Impact evaluation of environmental factors on respiratory function of asthma patients living in urban territory 全文
2018
Veremchuk, Lyudmila V. | Tsarouhas, Konstantinos | Vitkina, Tatyana I. | Mineeva, Elena E. | Gvozdenko, Tatyana A. | Antonyuk, Marina V. | Rakitskii, Valeri N. | Sidletskaya, Karolina A. | Tsatsakis, Aristidis M. | Golokhvast, Kirill S.
Environmental pollution, local climatic conditions and their association with the prevalence and exacerbation of asthma are topics of intense current medical investigation.Air pollution in the area of Vladivostock was estimated both by the index of emission volumes of "air gaseous components" (nitrogen oxide and nitrogen dioxide, formaldehyde, hydrogen sulfide, carbon monoxide) in urban atmosphere and by mass spectrometric analysis of precipitates in snow samples. A total of 172 local asthma patients (101 controlled-asthma patients–CAP and 71 non-controlled asthma patients – nCAP) were evaluated with the use of spirometry and body plethysmography. Airway obstruction reversibility was evaluated with the use of an inhaled bronchodilator. Using discriminant analysis the association of environmental parameters with clinical indices of asthma patients is explored and thresholds of impact are established.CAP presented high sensitivity to large-size suspended air particles and to several of the studied climatic parameters. Discriminant analysis showed high values of Wilks’ lambda index (α = 0.69–0.81), which implies limited influence of environmental factors on the respiratory parameters of CAP. nCAP were more sensitive and susceptible to the majority of the environmental factors studied, including air suspended toxic metals particles (Cr, Zn and Ni). Air suspended particles showed higher tendency for pathogenicity in nCAP population than in the CAP, with a wider range of particle sizes being involved. Dust fractions ranging from 0 to 1 μm and from 50 to 100 μm were additionally implicated compared to CAP group. Considerably lowest thresholds levels of impact are calculated for nCAP.
显示更多 [+] 显示较少 [-]Enhanced immobilization of U(VI) on Mucor circinelloides in presence of As(V): Batch and XAFS investigation 全文
2018
Song, Wencheng | Wang, Xiangxue | Chen, Zhongshan | Sheng, Guodong | Hayat, Tasawar | Wang, Xiangke | Sun, Yubing
The combined pollution of radionuclides and heavy metals has been given rise to widespread concern during uranium mining. The influence of As(V) on U(VI) immobilization by Mucor circinelloides (M. circinelloides) was investigated using batch experiments. The activity of antioxidative enzymes and concentrations of thiol compounds and organic acid in M. circinelloides increased to respond to different U(VI) and As(V) stress. The morphological structure of M. circinelloides changed obviously under U(VI) and As(V) stress by SEM and TEM analysis. The results of XANES and EXAFS analysis showed that U(VI) was mainly reduced to nano-uraninite (nano-UO2, 30.1%) in U400, while only 9.7% of nano-UO2 was observed in the presence of As(V) in U400-As400 due to the formation of uranyl arsenate precipitate (Trögerite, 48.6%). These observations will provide the fundamental data for fungal remediation of uranium and heavy metals in uranium-contaminated soils.
显示更多 [+] 显示较少 [-]Concurrent aggregation and transport of graphene oxide in saturated porous media: Roles of temperature, cation type, and electrolyte concentration 全文
2018
Wang, Mei | Gao, Bin | Tang, Deshan | Yu, Congrong
Simultaneous aggregation and retention of nanoparticles can occur during their transport in porous media. In this work, the concurrent aggregation and transport of GO in saturated porous media were investigated under the conditions of different combinations of temperature, cation type (valence), and electrolyte concentration. Increasing temperature (6–24 °C) at a relatively high electrolyte concentration (i.e., 50 mM for Na⁺, 1 mM for Ca²⁺, 1.75 mM for Mg²⁺, and 0.03 and 0.05 mM for Al³⁺) resulted in enhanced GO retention in the porous media. For instance, when the temperature increased from 6 to 24 °C, GO recovery rate decreased from 31.08% to 6.53% for 0.03 mM Al³⁺ and from 27.11% to 0 for 0.05 mM Al³⁺. At the same temperature, increasing cation valence and electrolyte concentration also promoted GO retention. Although GO aggregation occurred in the electrolytes during the transport, the deposition mechanisms of GO retention in the media depended on cation type (valence). For 50 mM Na⁺, surface deposition via secondary minima was the dominant GO retention mechanism. For multivalent cation electrolytes, GO aggregation was rapid and thus other mechanisms such as physical straining and sedimentation also played important roles in controlling GO retention in the media. After passing through the columns, the GO particles in the effluents showed better stability with lower initial aggregation rates. This was probably because less stable GO particles with lower surface charge densities in the porewater were filtered by the porous media, resulting in more stable GO particle with higher surface charge densities in the effluents. An advection–dispersion-reaction model was applied to simulate GO breakthrough curves and the simulations matched all the experimental data well.
显示更多 [+] 显示较少 [-]Differential effects of size-specific particulate matter on emergency department visits for respiratory and cardiovascular diseases in Guangzhou, China 全文
2018
Ge, Erjia | Lai, Kefang | Xiao, Xiong | Luo, Ming | Fang, Zhangfu | Zeng, Yanjun | Ju, Hong | Zhong, Nanshan
Studies differentiating the cardiorespiratory morbidity effects of PM₂.₅, PM₁₀, and PM₂.₅∼₁₀ (i.e. coarse PM or PMc) are still limited and inconsistent.To estimate the acute, cumulative, and harvesting effects of exposure to the three size-specific PM on cardiorespiratory morbidity, and their concentration-response relations.A total of 6,727,439 emergency department (ED) visits were collected from 16 public teaching hospitals in Guangzhou, from January 1st 2012 to December 31st 2015, among which over 2.1 million were asthma, COPD, pneumonia, respiratory tract infection (RTI), hypertension, stroke, and coronary heart disease (CHD). Distributed lag non-linear models (DLNM) was used to estimate the associations between the three size-specific PM and ED visits for the cardiovascular diseases. Long-term trends, seasonality, influenza epidemics, meteorological factors, and other gas pollutants, including SO2, NO₂, and O₃, were adjusted. We stratified the analyses by gender and age.Elevated PM₂.₅ and PM₁₀ were significantly associated with increased ED visits for pneumonia, RTI, and CHD at both lag₀ and lag₀₋₃. A 10 μg/m³ increment of PMc (at lag₀₋₁₄) was estimated to increase ED visits for pneumonia by 6.32% (95% CI, 4.19, 8.49) and for RTI by 4.72% (95% CI, 3.81, 5.63), respectively. PMc showed stronger cumulative effects on asthma in children than elderly. We observed significant harvesting effects (i.e. morbidity displacements) of the three size-specific PM on respiratory but very little on cardiovascular ED visits. The concentration-response curves suggested non-linear relations between exposures to the three different sizes of PM and respiratory morbidity.Overall, the three size-specific PM demonstrated distinct acute and cumulative effects on the cardiorespiratory diseases. PM₂.₅ and PMc would have significant effects on pneumonia and RTI. Strategies should be considered to further reduce levels of ambient PM₂.₅ and PMc.
显示更多 [+] 显示较少 [-]Assessment of light extinction at a European polluted urban area during wintertime: Impact of PM1 composition and sources 全文
2018
Vecchi, R. | Bernardoni, V. | Valentini, S. | Piazzalunga, A. | Fermo, P. | Valli, G.
In this paper, results from receptor modelling performed on a well-characterised PM₁ dataset were combined to chemical light extinction data (bₑₓₜ) with the aim of assessing the impact of different PM₁ components and sources on light extinction and visibility at a European polluted urban area. It is noteworthy that, at the state of the art, there are still very few papers estimating the impact of different emission sources on light extinction as we present here, although being among the major environmental challenges at many polluted areas. Following the concept of the well-known IMPROVE algorithm, here a tailored site-specific approach (recently developed by our group) was applied to assess chemical light extinction due to PM₁ components and major sources.PM₁ samples collected separately during daytime and nighttime at the urban area of Milan (Italy) were chemically characterised for elements, major ions, elemental and organic carbon, and levoglucosan. Chemical light extinction was estimated and results showed that at the investigated urban site it is heavily impacted by ammonium nitrate and organic matter. Receptor modelling (i.e. Positive Matrix Factorization, EPA-PMF 5.0) was effective to obtain source apportionment; the most reliable solution was found with 7 factors which were tentatively assigned to nitrates, sulphates, wood burning, traffic, industry, fine dust, and a Pb-rich source. The apportionment of aerosol light extinction (bₑₓₜ,ₐₑᵣ) according to resolved sources showed that considering all samples together nitrate contributed at most (on average 41.6%), followed by sulphate, traffic, and wood burning accounting for 18.3%, 17.8% and 12.4%, respectively.
显示更多 [+] 显示较少 [-]Quantifying the influence of natural and socioeconomic factors and their interactive impact on PM2.5 pollution in China 全文
2018
Yang, Dongyang | Wang, Xiaomin | Xu, Jianhua | Xu, Chengdong | Lu, Debin | Ye, Chao | Wang, Zujing | Bai, Ling
PM2.5 pollution is an environmental issue caused by multiple natural and socioeconomic factors, presenting with significant spatial disparities across mainland China. However, the determinant power of natural and socioeconomic factors and their interactive impact on PM2.5 pollution is still unclear. In the study, the GeogDetector method was used to quantify nonlinear associations between PM2.5 and potential factors. This study found that natural factors, including ecological environments and climate, were more influential than socioeconomic factors, and climate was the predominant factor (q = 0.56) in influencing PM2.5 pollution. Among all interactions of the six influencing factors, the interaction of industry and climate had the largest influence (q = 0.66). Two recognized major contaminated areas were the Tarim Basin in the northwest region and the eastern plain region; the former was mainly influenced by the warm temperate arid climate and desert, and the latter was mainly influenced by the warm temperate semi-humid climate and multiple socioeconomic factors. The findings provided an interpretation of the influencing mechanisms of PM2.5 pollution, which can contribute to more specific policies aimed at successful PM2.5 pollution control and abatement.
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