The occurrence of microplastics throughout marine environments worldwide, from pelagic to benthic habitats, has become serious cause for concern. Hadal zones were recently described as the “trash bins of the oceans” and ultimate sink for marine plastic debris. The Kuril region covers a substantial area of the North Pacific Ocean and is characterised by high biological productivity, intense marine traffic through the Kuril straits, and anthropogenic activity. Moreover, strong tidal currents and eddy activity, as well as the influence of Pacific currents, have the potential for long distance transport and retention of microplastics in this area. To verify the hypothesis that the underlying Kuril Kamchatka Trench might accumulate microplastics from the surrounding environments and act as the final sink for high quantities of microplastics, we analysed eight sediment samples collected in the Kuril Kamchatka Trench at a depth range of 5143–8250 m during the Kuril Kamchatka Biodiversity Studies II (KuramBio II) expedition in summer 2016. Microplastics were characterised via Micro Fourier Transform Infrared spectroscopy. All samples were analysed in their entirety to avoid inaccuracies due to extrapolations of microplastic concentrations and polymer diversities, which would otherwise be based on commonly applied representative aliquots. The number of microplastic particles detected ranged from 14 to 209 kg−1 sediment (dry weight) with a total of 15 different plastic polymers detected. Polypropylene accounted for the largest proportion (33.2%), followed by acrylates/polyurethane/varnish (19%) and oxidized polypropylene (17.4%). By comparing extrapolated sample aliquots with in toto results, it was shown that aliquot-based extrapolations lead to severe under- or overestimations of microplastic concentrations, and an underestimation of polymer diversity.

Noise pollution is a pervasive factor that increasingly threatens natural resources and human health worldwide. In particular, large-scale changes in road networks have driven shifts in the acoustic environment of rural landscapes during the past few decades. Using sampling plots from the Spanish Landscape Monitoring System (SISPARES), 16 km2 each, we modelled the spatio-temporal changes in road traffic noise pollution in Ecoregion 1 of Spain (approximately 66,000 km2). We selected a study period that was characterised by significant changes in the size of the road network and the vehicle fleet (i.e. between 1995 and 2014) and used standard and validated acoustic computation methods for environmental noise modelling (i.e. European Directive, 2002/49/EC) within sampling plots. We then applied a multiple linear regression to expand noise modelling throughout the whole of Ecoregion 1. Our results showed that the noise level increased by 1.7 dB(A) in average per decade in approximately 65% of the territory, decreased by 1.3 dB(A) per decade in about 33%, and remained unchanged in 2%. This suggests that road traffic noise pollution levels may not grow homogeneously in large geographical areas, maybe due to the concentration of large fast traffic flows on modern motorways connecting towns. Our research exemplifies how landscape monitoring systems such as cost-effective approaches may play an important role when assessing spatio-temporal patterns and the impact of anthropogenic noise pollution at large geographical scales, and even more so in a global context of constricted resources and limited availability of historical data on traffic and environmental noise monitoring.

Embargo until February 12, 2022 | Cost-efficient and environmentally friendly treatment of hydraulic fracturing effluents is of great significance for the sustainable development of shale gas exploration. We investigated the synergistic effects of plant-microbial treatment of shale gas fracturing waste fluid. The results showed that illumination wavelength and temperature are direct drivers for microbial treatment effects of CODCr and BOD5, while exhibit little effects on nitrogen compounds, TDS, EC, and SS removals as well as microbial species and composition. Plant-microbial synergism could significantly enhance the removal of pollutants compared with removal efficiency without plant enhancement. Additionally, the relative abundance and structure of microorganisms in the hydraulic fracturing effluents greatly varied with the illumination wavelength and temperature under plant-microbial synergism. 201.24 g water dropwort and 435 mg/L activated sludge with illumination of 450–495 nm (blue) at 25 °C was proved as the best treatment condition for shale gas fracturing waste fluid samples, which showed the highest removal efficiency of pollutants and the lowest algal toxicity in treated hydraulic fracturing effluents. The microbial community composition (36.73% Flavobacteriia, 25.01% Gammaproteobacteria, 18.55% Bacteroidia, 9.3% Alphaproteobacteria, 4.1% Cytophagia, and 2.83% Clostridia) was also significantly different from other treatments. The results provide a potential technical solution for improved treatment of shale gas hydraulic fracturing effluents. | acceptedVersion

International audience | Many toxicants are present in water as a mixture. Male infertility is one of the environmental impacts in developed countries. Using our rat seminiferous tubule culture model, we evaluated the effects of waters of different origins, on several parameters of the seminiferous epithelium. Concentrated culture medium was diluted with the waters to be tested (final concentrations of the tested waters were between 8 and 80%). The integrity of the blood-testis barrier was assessed by the trans-epithelial electric resistance (TEER). The levels of mRNAs specific of Sertoli cells, of cellular junctions, of each population of germ cells, of androgen receptor, of estrogen receptor α and of aromatase were also studied. We report, here, the results obtained with ten waters, some of them possessing a negative effect on spermatogenesis. The results showed that according to the tested waters, their effects on the parameters studied might be quite different indicating many different mechanisms of toxicity, including some endocrine disrupting effects. It has been reported that men with impaired semen parameters have an increased mortality rate suggesting semen quality may provide a fundamental biomarker of overall male health. Hence, we have developed a relevant in vitro bioassay allowing to evaluate the potential toxicity of different types of waters on male fertility and to assess some aspects of their mechanism of action. In addition to the TEER measure, the number and/or the identity of the studied mRNAs can be largely increased and/or modified, thus enhancing the possibility of using this model as a "warning system".

In marine settings, anthropogenic disturbances and climate change increase the rate of biological invasions. Predicting still undescribed invasive alien species (IAS) is needed for preparing timely management responses. We tested a strategy for discovering new potential IAS using DNA in a trans-equatorial expedition onboard RV Polarstern. During one-month travel, species inside ballast water experienced oxygen depletion, warming, darkness and ammonium stress. Many organisms died but several phytoplankton and zooplankton survivors resisted and were detected through a robust combination of individual sampling, DNA barcoding and metabarcoding, new in ballast water studies. Ammonium was identified as an important influential factor to explain diversity changes in phytoplankton and zooplankton. Some species reproduced until the end of the travel. These species tolerant to travel stress could be targeted as potential IAS and prioritized for designing control measures. Introducing resistance to travel stress in biosecurity risk analysis would be recommended.

International audience

Despite an increasing understanding of the issue of marine pollution, humanity continues on a largely unsustainable trajectory. This study aimed to identify and classify the range of scientific studies and interventions to address coastal and marine pollution. We reviewed 2417 scientific papers published between 2000 and 2018, 741 of which we analysed in depth. To classify pollution interventions, we applied the systems-oriented concept of leverage points, which focuses on places to intervene in complex systems to bring about systemic change. We found that pollution is largely studied as a technical problem and fewer studies engage with pollution as a systemic social-ecological issue. While recognising the importance of technical solutions, we highlight the need to focus on under-researched areas pertaining to the deeper drivers of pollution (e.g. institutions, values) which are needed to fundamentally alter system trajectories.

International audience