Copper hydrotalcites with and without adsorbed chlorophyllin exhibit a bactericidal effect that depends on the copper release and the basicity, which can be tuned through the chlorophyllin adsorption. The prepared solids performed well for the elimination of Escherichia coli, Enterobacter aerogenes, Salmonella enterica, and Staphylococcus aureus bacteria. The results showed that the copper-containing hydrotalcite with the adsorbed chlorophyllin is the most active material. Wastewaters from a metal industry were treated with these hybrid compounds, and the bactericidal effect was comparable with the results reported using more complex methods such as photocatalysis. Furthermore, one main advantage of these hybrid compounds is its low human toxicity compared with silver-containing materials.

This work is a synthesis of a 5-year estimation of nitrogen balance at a semi-arid, semi-natural, undisturbed grassland site (Bugac). We measured the N input of atmospheric pollutants by wet and dry deposition of gases and aerosols, while we considered N output as NO and N₂O gases volatilized from soil. Besides measurements of soil fluxes, the denitrification-decomposition (DNDC) ecological model was also used and simulations were compared to and validated against the measured values. The daily flux simulations generally did not match well the measured data for N₂O and NO. In most cases, the mean fluxes were underestimated, though results of the comparison of monthly values suggest that model data, together with observed deposition data, are applicable to estimate the net N balance for grasslands. The calculated yearly N balance (net flux) between atmosphere and surface, without biological fixation and effect of grazing, ranged between −9.4 and −14 kg N ha⁻¹ year⁻¹as the sum of the measured deposition and emission terms, −11 to −15 and 0.9 to 2.9 kg N ha⁻¹ year⁻¹, respectively, between 2006 and 2010. Observed and modeled soil emissions were lower by one order of magnitude than atmospheric deposition. Considering the biological nitrogen fixation and the effect of grazing (effects of both grazed plant and excreta), the net nitrogen balance varies within −6.6 and −11 kg N ha⁻¹ year⁻¹. It seems — taking into account the high uncertainty in calculation due to the effect of grazing — that sources of nitrogen exceed the sinks; the surplus is probably mineralized in the soil.

Methane production by methanogenic microbes under anaerobic condition is affected by the types of fertilizers, which determine carbon availability, used in rice fields. In addition, irrigation management controls oxygen availability in soil. Thus, irrigation management and types of fertilizers are major driving forces for methane emission in rice fields. While these factors affect paddy microbial communities over the course of cultivation, little is known about the effects of fertilizers and irrigation conditions on initial paddy microbial communities. In this study, we investigated the initial impacts of fertilizers and irrigation systems on paddy microbial communities and methane emission. At early stages of rice cultivation (2 weeks after transplanting 15-day-old rice seedlings), a high amount of methane was emitted from rice fertilized with swine manure. In addition, pre-transplantation flooding increased methane emission by 30 %. Although these conditions did not affect the overall paddy soil microbial communities, 126 operational taxonomic units (OTUs) were found to be significantly more abundant in paddy soils fertilized with swine manure. These OTUs included archaeal methanogenic species and bacterial substrate providers for biomethane production. Shared-OTU analysis with swine fecal microbial communities indicated swine manure as the origin of key methane-producing microbes. In conclusion, the applications of swine manure and permanent flooding irrigation introduce active methane producers and enhance methane emission, respectively, and should therefore be avoided.

The aim of this work is to increase the information on trace metals in seabirds and coastal soils in the Antarctica. Concentrations (mg kg⁻¹dry weight) of Cd, Cu, Cr, Ni, Mn, Zn and Pb were determined by ICP-MS in fresh excreta and feathers of Gentoo penguins as well as in soils around the nesting sites where this species inhabits. Samples were collected in four locations throughout the Antarctic Peninsula (January 2014): O’Higgins Base, Stranger Point, Neko Harbor and Doumer Island. The highest levels of elements were found in excreta from O’Higgins Base (2.92, 266.83, 2.99, 44.75, 18.15, 1.68 and 317.92 for Cd, Cu, Cr, Mn, Ni, Pb and Zn, respectively) and Stranger Point (1.97, 222.51, 2.98, 36.62, 13.41, 1.46 and 201.18 for Cd, Cu, Cr, Mn, Ni, Pb and Zn, respectively. Similarly, the highest levels were found in feathers from O’Higgins Base (0.21, 20.89, 1.44, 1.19, 5.90, 0.63 and 64.07 for Cd, Cu, Cr, Mn, Ni, Pb and Zn, respectively) and Stranger Point (0.14, 19.65, 1.47, 1.23, 3.85, 0.60 and 64.19 for Cd, Cu, Cr, Mn, Ni, Pb and Zn, respectively). In soils, the highest levels were found in O’Higgins Base (4.31, 421.94, 64.75, 404.76, 28.13, 281.54 and 484.99 for Cd, Cu, Cr, Mn, Ni, Pb and Zn, respectively), whereas the lowest levels were found in Neko Harbor and Doumer Island. These results observed could be related to the major human presence in the northern area of the Antarctic Peninsula and large-scale transport of pollutants. The metals detected in the excreta of the Gentoo penguin can contribute to increase the contamination of coastal terrestrial ecosystems, which could also affect other living organisms.

Degradation and fate of sulfamethazine (SMZ) were determined under aerobic and anaerobic conditions in soil with and without swine manure amendment. For both aerobic and anaerobic conditions, SMZ disappeared rapidly during the first 7 days followed by slow disappearance which may indicate that SMZ had become more persistent and less available. For soils receiving 100 mg/kg of SMZ, the percent of SMZ remaining in the soil after 63 days were between 25 and 60 %. Depending on the initial SMZ concentration, estimated half-lives for aerobic and anaerobic incubations ranged from 1.2 to 6.6 and 2.3 days to more than 63 days, respectively. Addition of manure (0.054 g/g soil) did not significantly affect the half-lives of SMZ. Inhibitory effects of SMZ on anaerobic microbial respiration were observed in unamended soil at concentrations of 50 mg/kg or higher, but only transient inhibitory effects were found in aerobic soil. Five to 22 % of the¹⁴C[phenyl]-SMZ added were extracted at the end of the incubations while 70 to 91 % of the¹⁴C were converted to bound (non-extractable) forms in both manure amended and unamended soil. Only 0.1 to 1.5 % of¹⁴C-SMZ was mineralized to¹⁴CO₂. Disappearance of SMZ in sterilized soil was not completely halted indicating possible contribution of abiotic processes to the disappearance of SMZ in soil.

Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl₂) was evaluated in Limnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 μM), ET (0.004. 0.04, 0.4 and 4 μM) and CdCl₂ (0.004, 0.04, 0.4, 4 and 40 μM). Mortality of worms was observed only for CdCl₂ (4 and 40 μM). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 μM), ET (4 μM) and CdCl₂ (0.4 and 4 μM). All tested substances induced significant increase of DNA damage except 0.004 μM of ET. L. udekemianus being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 μM induced DNA damage is similar to ones detected in wastewaters.

The aboveground tissue of plants is important for providing roots with constant photosynthetic resources. However, the aboveground biomass is usually harvested before winter to maintain the permanent removal of nutrients. In this work, the effects of harvest on plants’ involvement in oxygen input as well as in microbial abundance and activity were investigated in detail. Three series of constructed wetlands with integrated plants (“unharvested”), harvested plants (“harvested”), and fully cleared plants (“cleared”) were set up. Better performance was found in the unharvested units, with the radial oxygen loss (ROL) rates ranging from 0.05 to 0.59 μmol O₂/h/plant, followed by the harvested units that had relatively lower ROL rates (0.01 to 0.52 μmol O₂/h/plant). The cleared units had the lowest removal efficiency, which had no rhizome resources from the plants. The microbial population and activity were highest in the unharvested units, followed by the harvested and cleared units. Results showed that bacterial abundances and enhanced microbial activity were ten times higher on root surfaces compared with sands. These results indicate that late autumn harvesting of the aboveground biomass exhibited negative effects on plant ROL as well as on the microbial population and activity during the following winter.

We need specific and competent adsorbents to remove arsenic and bring it down to permissible levels in drinking water. Therefore, industrial byproducts are extensively applied to produce large amounts of natural adsorbents. Similarly, managing optimum arsenic adsorption with palm oil clinker sand (POCS) is possible through a careful statistical planning of adsorption variables. We plan and perform a minimum number of experiments to (1) obtain optimum arsenic adsorption and (2) provide a new possible application opportunity to the industrial waste managers and future planners. We observed that adsorption of arsenic was dependent on the pH of the system, initial concentration of arsenic (mg L⁻¹), amount (mg) of POCS, and temperature of the bio-adsorption system. A correlation among the study variables was constructed by three-dimensional (3D) response surfaces and two-dimensional (2D) contour plots based on central composite design (CCD) experiments in a batch mode of study. A quadratic model fitted well with the experimental data and better explained the superiority of current bio-adsorption system and efficient removal of arsenic from water samples. We confirmed that the selected variables were experimentally and statistically significant and controlled the overall adsorption response by the batch system. A comparative and thorough analysis of the adsorption process confirmed that selected variables were mutually interacting in a nonlinear fashion in this study. Excellent experimental results and external comparative studies prove the relative importance of the present model and adsorption system for arsenic remediation biotechnology.

SrCO₃ was formed and added as a carrier into copper-based catalyst (CuZnAl catalyst) prepared by hydrothermal method before the catalyst incorporates with HZSM-5. The CuZnAlSr catalyst was characterized by SEM, BET, XRD, IR, and activity-evaluation system in a fixed-bed tubular reactor equipped with chromatograph (GC). The conversion of CO₂ reaches 30.30 %, and the overall yield of methanol and dimethyl ether is 27.80 %. Catalytic property as to CO₂ conversion has only slight decrease even up to 150 h of reaction time. The addition of SrCO₃ enhanced the activity of the catalyst through providing a tridimensional frame and electron transfer bridge.

The present work studies the remediation of a B20 (20 % biodiesel, 80 % diesel) biodiesel blend-contaminated soil (1,000 mg kg⁻¹) with persulfate activated by iron. Three different sources of iron (Fe(II)), granular zerovalent iron (gZVI), and a slurry of nanoparticles of zerovalent iron (nZVI), without pH adjustment were tested. Besides, the effect of the addition of chelating agents, such as trisodium citrate (SC), or citric acid (CiA), has been also studied. SC promotes pH under near-neutral conditions and reaction takes place at low rate at these experimental conditions. On the other hand, the use of CiA leads to an acidic pH and chelating agent is oxidized at higher rate than total petroleum hydrocarbons (TPH). Therefore, CiA addition does not seem to produce any improvement on the removal efficiency of TPH. Regarding the three different sources of iron used as activators, Fe(II), gZVI and nZVI, in absence of chelating agent, under acidic pH and by adding the same amount of iron, the highest TPH conversion was obtained with ZVI (about 60 %), while a conversion of about 40 % was obtained with the addition of Fe(II). The maximum TPH conversion value was achieved in shorter time using nZVI. Concerning the removal efficiency of each fraction of biodiesel abated, fatty acid methyl esters (FAME) were by far the easiest to oxidize, achieving 100 % of conversion, either by using Fe(II) or nZVI activated persulfate.