Microplastics are small plastic particles, globally distributed throughout the oceans. To properly study them, all the methodologies for their sampling, extraction, and measurement should be standardized. For heterogeneous samples containing sediments, animal tissues and zooplankton, several procedures have been described. However, definitive methodologies for samples, rich in algae and plant material, have not yet been developed. The aim of this study was to find the best extraction protocol for vegetal-rich samples by comparing the efficacies of five previously described digestion methods, and a novel density separation method. A protocol using 96% ethanol for density separation was better than the five digestion methods tested, even better than using H2O2 digestion. As it was the most efficient, simple, safe and inexpensive method for isolating microplastics from vegetal rich samples, we recommend it as a standard separation method.

Seagrasses are among the most productive shallow water ecosystems, serving a diverse assemblage of fish and invertebrates. Tropical seagrass communities are dominated by the turtle grass Thalassia testudinum, whose wide, flattened blades host diverse epibiont communities. Amidst its epibionts, T. testudinum may also be accumulating microplastics, which are a ubiquitous marine pollutant even in remote locales. To assess the extent of microplastic accumulation, seagrass samples were collected from Turneffe Atoll, which lies offshore but parallel with a major urban center. Seventy-five percent of Thalassia blades had encrusted microplastics, with microfibers occurring more than microbeads and chips by a ratio of 59:14. Grazers consumed seagrasses with higher densities of epibionts. Potential mechanisms for microplastic accumulation include entrapment by epibionts, or attachment via biofilms. This study is the first to document microplastics on marine vascular plants, suggesting that macroherbivory is a viable pathway for microplastic pollution to enter marine food webs.

The presence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in oceanic ecosystems constitutes an emerging public health risks in the marine environment. In this study, we report for the first time the identification of ESBL (CTX-M)-producing E. coli in wild fishes from a polluted area in the South Atlantic coast of Brazil, where a genomic analysis confirm the presence of livestock and human E. coli lineages belonging to sequence types (STs) ST744 and ST746, which carried clinically relevant resistance genes for human and veterinary antibiotics, and heavy metals. These findings reveal the presence of multidrug-resistant (MDR) bacteria in the gut microbiota of wild fishes living in polluted coastal waters, alerting that microbial contamination by bacteria related directly and indirectly to human or animal activities could affect the safety of the seafood supply, as well as the commercial and recreational use of coastal marine waters.

Statistical analysis of rainfall data from 2005 to 2015 showed that atmospheric deposition supplied large amount of dissolved inorganic nitrogen (38–155 mg·m−2·month−1) in N-deficient South China Sea and Eastern Indian Ocean. To understand marine ecosystem responses to increasing nutrient disturbances, we implemented field mesocosm experiments to study phytoplankton community structure and biodiversity responses to nutrient treatments with nitrate, phosphate and iron across tropical seas. Our results showed that DIN supply would change phytoplankton community structure and stimulated the regime shift from cyanobacteria to diatoms (relative dominance R > 0). Phytoplankton communities were dominated by diatoms (relative abundance >50%) accompanied by high chlorophyll a content with 1.58–39.27 μg·L−1 in DIN-added cultures, whereas cyanobacteria dominated communities (relative abundance >60%) with low biomass of 0.12–0.18 μg·L−1 in undisturbed cultures. Simultaneously increased DIN loading from atmospheric deposition would decrease ecological diversity of tropical seas owing to species competition and succession (Shannon diversity H′ decreased to <1).

Conventional methods for determination of polycyclic aromatic compounds (PACs) in sediments usually require large sample sizes (grams) and solvent volumes (at least 100 mL) through the employment of Soxhlet extraction, which is both time (hours) and energy consuming, among other disadvantages. We developed a new analytical protocol for the determination of PACs in sediments using microextraction, which requires small sample masses (25 mg), 500 μL of acetonitrile-dichloromethane mix and sonication for 23 min, followed by GC–MS analysis. The method was validated using the certified reference material SRM 1941b – NIST organic marine sediment, as well as internal deuterated standards. Seventeen PAHs, seven nitro-PAHs and one quinone were detected and quantified. The mean concentrations were 90.4 ng g−1 for PAHs, 179.2 ng g−1 for nitro-PAHs and 822.5 ng g−1 for quinones. The proposed method showed good sensitivity, linearity, precision and accuracy for the determination of PAC in sediments samples.

The performance of oil spill models is strongly influenced by multiple parameters. In this study, we explored the ability of a genetic algorithm (GA) to determine optimal parameters without the need for time-consuming manual attempts. An evaluation function integrating the percentage of coincidence between the predicted polluted area and the observed spill area was proposed for measuring the performance of a Lagrangian oil particle model. To maximise the objective function, the oil spill was run numerous times with continuously optimised parameters. After many generations, the GA effectively reduced discrepancies between model results and observations of a real oil spill. Subsequent validation indicated that the oil spill model predicted oil slick patterns with reasonable accuracy when equipped with optimal parameters. Furthermore, multiple objective optimisation for observations at different times contributed to better model performance.

Vessel slowdown may be an alternative mitigation option in regions where re-routing shipping corridors to avoid important marine mammal habitat is not possible. We investigated the potential relief in masking in marine mammals and fish from a 10 knot speed reduction of container and cruise ships. The mitigation effect from slower vessels was not equal between ambient sound conditions, species or vessel-type. Under quiet ambient conditions, a speed reduction from 25 to 15 knots resulted in smaller listening space reductions by 16–23%, 10–18%, 1–2%, 5–8% and 8% respectively for belugas, bowheads, bearded seals, ringed seals, and fish, depending on vessel-type. However, under noisy conditions, those savings were between 9 and 19% more, depending on the species. This was due to the differences in species' hearing sensitivities and the low ambient sound levels measured in the study region. Vessel slowdown could be an effective mitigation strategy for reducing masking.

The eutrophication status of the German Bight (North Sea) has been assessed the third time since 1998 according to the OSPAR-Comprehensive Procedure between 2006 and 2014. Since the 1980s nutrient discharges and atmospheric nitrogen deposition had declined significantly but chlorophyll a and nutrient concentrations remained above assessment levels inshore and in inner coastal waters, reflecting continuing eutrophication. Recently local river discharges stagnated or increased again and total nitrogen remained above a reduction target of 200 μM. Most nutrients and conversion products were imported by a coastal current, passing the German Bight. Organic matter was trapped in offshore bottom waters in the ancient Elbe valley, causing repeated annual oxygen minima (<6 mg/L) and a classification as Problem Area. Effects of national reduction measures are limited in the transit area German Bight because improvements in open coastal waters require international efforts, based on comprehensive analyses.

The distribution characteristics, sources and ecological risk of antimony (Sb) in the surface sediments of Changjiang Estuary and the adjacent sea were studied. Sb concentrations ranged from 0.320 to 0.968 μg g−1 with mean value of 0.577 μg g−1. Sb concentrations were relatively high in sediments of the south Yellow Sea, the Hangzhou Bay mouth and the inner Changjiang Estuary. The variation trend of Sb concentrations was controlled by hydrodynamics, Al/Fe/Mn oxides. Sb also showed strong chalcophile property. Correlation analysis and enrichment factor showed Sb came mainly from natural sources. Total Sb sediment flux in the study area was 446.3 t/yr. The Changjiang River, the Yellow River and atmospheric inputs accounted for 85.7%, 13.9%, and 0.4% of the total sediment Sb flux, respectively. The result of potential ecological index indicated the very low Sb concentrations could hardly threat the ecological environment of the study area.

Severe microplastic pollution from anthropogenic activities in coastal zones presents an imminent risk to marine ecosystems. In this study, abundant microplastics (15–12,852 items kg−1) with sizes ranging between 0.16 and 5.0 mm were extracted from 17 sediment samples collected in sandy beaches and mangrove wetlands of the Qinzhou Bay, Guangxi Province, Southwest China. Three types of microplastics (i.e. polystyrene, polypropylene, and polyethylene) were identified with Fourier transform infrared (FTIR) spectroscopy analysis. These detected microplastics were characterized by different colors (white, transparent, yellow, green, red, and blue) and shapes (fragment, fiber, and sphere). Microplastics were concentrated on supratidal beaches and wetlands outside of mangrove, and less abundant on intertidal beaches and inside of mangrove wetlands. Meanwhile, high microplastic concentrations were observed near mollusk farms. The spatial distribution and chemical speciation indicated that microplastics were derived from disintegration of large plastic debris (e.g., Styrofoam buoys used to support mollusk rafts) abandoned by aquaculture industry. Further, coastal vegetation (e.g. mangrove) could trap microplastic particles.