Bacteria with arsenate-reducing (ars) and arsenite-oxidizing (aio) genes usually co-exist in aerobic environments, but their contrast impacts on arsenic (As) speciation and mobility remain unclear. To identify which kind of bacteria dominate As speciation under oxic conditions, we studied the biotransformation of adsorbed As on goethite in the co-existence of Pantoea sp. IMH with ars gene and Achromobacter sp. SY8 with aio gene. The incubation results show that SY8 dominated the dissolved As speciation as As(V), even though aio exhibited nearly 5 folds lower transcription levels than ars in IMH. Nevertheless, our XANES results suggest that SY8 showed a negligible effect on solid-bound As speciation whereas IMH reduced adsorbed As(V) to As(III). The change in As speciation on goethite surfaces led to a partial As structural change from bidentate corner-sharing to monodentate corner-sharing as evidenced by our EXFAS analysis. Our Mössbauer spectroscopic results suggest that the incubation with SY8 reduced the degree of crystallinity of goethite, and the reduced crystallinity can be partly compensated by IMH. The changes in As adsorption structure and in goethite crystallinity had a negligible effect on As release. The insights gained from this study improve our understanding of biotransformation of As in aerobic environment.

Manganese (Mn) transporter OsNRAMP5 was widely reported to regulate cadmium (Cd) uptake in rice. However, the relationship between OsNRAMP5 expression level and Cd accumulation, impacts of external ion activities on OsNRAMP5 expression level and Cd accumulation are still unclear. Investigations of the relationship between OsNRAMP5 expression level and Cd accumulation in three indica rice genotypes were conducted under various external Mn²⁺ activities ranging from Mn deficiency to toxicity in EGTA-buffered nutrient solution. Results in this work indicated that OsNRAMP5 expression level in roots significantly up-regulated at Mn phytotoxicity compared to that at Mn deficiency, which may stimulate by the increasing uptake of Mn. Our work also demonstrated that root Cd concentration of all the tested rice decreased notably when external Mn²⁺ activity reached the level of toxicity. This may explain by the increasing competition between the excess Mn²⁺ and Cd²⁺ as well as the disorder of element absorption caused by root damage at Mn toxicity. Our work also revealed that the relationship between OsNRAMP5 expression level in roots and Cd accumulation in roots was insignificant for all the tested genotypes. Besides, OsNRAMP5 expression level in roots seemed more related to root Mn accumulation. The fact that function of OsNRAMP5 mainly focuses on Mn uptake, together with the fact that many transporter genes involved in Cd uptake might result in the insignificant correlation between OsNRAMP5 expression level and Cd accumulation in roots. At last, multi-level regulating and processing of the process from gene expression to protein translation might account for the inconsistent relationship between root OsNRAMP5 expression level and Cd accumulation in roots.

Plant-specific root-microbe-soil interactions play an indisputable role in microbial adaptation to environmental stresses. However, the assembly of plant rhizosphere microbiomes and their feedbacks in modification of pollution alleviation under organochlorine stress condition is far less clear. This study examined the response of root-associated bacterial microbiomes to lindane pollution and compared the dissipation of lindane in maize-cultivated dry soils and rice-cultivated flooded soils. Results showed that lindane pollution dramatically altered the microbial structure in the rhizosphere soil of maize but had less influence on the microbial composition in flooded treatments regardless of rice growth, when the reductive dechlorination of lindane was actively coupled with natural redox processes under anaerobic conditions. After 30 days of plant growth, lindane residues dissipated much faster in anaerobic than in aerobic environments, with only 1.08 mg kg⁻¹ lindane remaining in flooded control compared to 12.79 mg kg⁻¹ in dry control soils. Compared to the corresponding unplanted control, maize growth significantly increased, but rice growth slightly decreased the dissipation of lindane. Our study suggests that opposite impacts would lead to the self-purification of polluted soils during the growth of xerophytic maize and hygrocolous rice. This was attributed to the contrasting belowground micro-ecological processes regarding protection of root tissues and thereby assembly of rhizosphere microbiomes shaped by the xerophytic and hygrocolous crops under different water managements, in response to lindane pollution.

Traditional toxicity tests assess stressor effects on individuals, while protection goals are focused on the population-level and above. Additionally, these tests ignore common ecological factors such as resource levels and population growth phase. The objective of this research was to explore effects of – and interactions between – resource availability and stress response at the individual and population levels using Daphnia magna as a model. We hypothesized that density-dependent changes in resources at various phases of population growth would cause different population responses to the same toxicant stress. Laboratory populations of Daphnia magna were exposed to a 48-h pulse of 20 or 30 μg/l pyraclostrobin in one of four distinct phases of laboratory population cycles: growth, peak, decline, and stable. Population size and recovery were observed throughout the 51-day study. Populations exposed to pyraclostrobin during the growth phase had the least mortality and fastest recovery, while populations in the peak phase had the greatest mortality and slowest recovery. These data suggested that high density and low food at the peak phase resulted in more sensitive daphnids. To further test this hypothesis, a resource-amended acute toxicity study was conducted to quantify the effects of food resource on pyraclostrobin toxicity to Daphnia magna. Three age classes of Daphnia magna (neonate, subadult, adult) were fed low or high food levels and exposed to pyraclostrobin for 48 h. Toxicity was greater, as shown by lower 48 h LC50s, for smaller Daphnia magna age classes and lower food levels comporting results in the population study. Importantly, the acute toxicity studies generally yielded lower effect levels than the population studies suggesting that while the standard acute studies are ecologically unrealistic, they may be protective of toxicity under some circumstances. Collectively, these data point to the importance of population phase and the resource environment in modulating toxicity.

Malaysia is a tropical country that is highly dependent on surface water for its raw water supply. Unfortunately, surface water is vulnerable to pollution, especially in developed and dense urban catchments. Therefore, in this study, a methodology was developed for an extensive temporal water quality index (WQI) and classification analysis, simulations of various pollutant discharge scenarios using QUAL2K software, and maps with NH₃–N as the core pollutant using an integrated QUAL2K-GIS. It was found that most of the water quality stations are categorized as Class III (slightly polluted to polluted). These stations are surrounded by residential areas, industries, workshops, restaurants and wet markets that contribute to the poor water quality levels. Additionally, low WQI values were reported in 2010 owing to development and agricultural activities. However, the WQI values improved during the wet season. High concentrations of NH₃–N were found in the basin, especially during dry weather conditions. Three scenarios were simulated, i.e. 10%, 50% and 70% of pollution discharge into Skudai river using a calibrated and validated QUAL2K model. Model performance was evaluated using the relative percentage difference. An inclusive graph showing the current conditions and pollution reduction scenarios with respect to the distance of Skudai river and its tributaries is developed to determine the WQI classification. Comprehensive water quality maps based on NH₃–N as the core pollutant are developed using integrated QUAL2K-GIS to illustrate the overall condition of the Skudai river. High NH₃–N in the Skudai River affects water treatment plant operations. Pollution control of more than 90% is required to improve the water quality classification to Class II. The methodology and analysis developed in this study can assist various stakeholders and authorities in identifying problematic areas and determining the required percentage of pollution reduction to improve the Skudai River water quality.

Improper land-use changes may lead to a loss of soil resources and cause environmental pollution. Chinese Torreya plantation (hereafter CTP) is an important cash tree plantation for nuts production in the mountainous areas of subtropical China. The increasing development of CTPs, to increase seed production, can result in the complete erasure of local natural vegetation.In this study, the vulnerability to soil erosion, loss of soil organic carbon (SOC) and nutrients in CTPs due to land-use change were evaluated. The results indicated that the rates of diffusive soil erosion in the young CTPs with extreme precipitation were about six-fold higher than with the natural vegetation. At sites with a similar slope, there was no significant difference in soil erosion levels between the young and old CTPs. The old CTPs did not hold significantly higher levels of SOC and soil total nitrogen (STN) in their topsoil when compared with the young CTPs. The natural mixed broadleaved subtropical forests lost about 35% of their SOC and 25% of their STN after they were converted into CTPs, but the CTPs had higher soil total phosphorus. The C: N ratios at the different sites were close to 11:1, but the N: P ratios were diverse. There were high levels of organic carbon, nitrogen and phosphorus in stream water. Adequate coverage of natural vegetation within or around the CTPs should be maintained to decrease soil erosion and nutrient loss. Suggestions to develop CTPs while protecting the environment are discussed. Overall, it was determined that aspects of the current management practices and strategies for developing CTPs should be changed to decrease soil erosion and nutrient loss.

Supported carbon quantum dots (CQDs), used as fluorescent sensors for the detection of metal ions, have rarely been used to remove heavy metals from water. Nitrogen-doped CQDs immobilized in hydrophilic silica hydrogels exhibited a more superior sensitivity and selectivity for the detection of Re(VII) and Cr(VI) than other metal ions, including Fe(III), Fe(II), Zn(II), Cu(II) and Mn(II). For the first time, low limits of detection (LOD) of 2.3 μM for Re(VII) detection and 65 nM for Cr(VI) detection were reported by a facile method. Based on the high selectivity of fluorescent silica hydrogels for Re(VII) and Cr(VI) detection, the removal of Re(VII) and Cr(VI) by graphene oxide (GO) in water was monitored with the hydrogels used as a turn-off fluorescent sensing platform. The consistent results of the sorption isotherms of each metal on GO, which were obtained from the fluorescence spectra and by UV absorption, further verified the possibility of monitoring metal removal by fluorescence detection. Remarkably, GO removed 1186 mg/g of Re(VII) but only 178 mg/g of Cr(VI). The density functional theory (DFT) calculations indicated that both Re(VII) and Cr(VI) formed stable bonds with silica hydrogels, confirming that the interactions between the metal ions and the substrate would promote the fluorescence quenching of the supported CQDs. On the other hand, Re(VII) interacted more strongly with the carboxyl groups of GO than Cr(VI). In addition, a real-time detection system was designed to alarm the service life of a GO filter used for Re(VII) removal.

A multinational demersal longline survey was conducted on the Gulf of Mexico continental shelf over the years 2015 and 2016 to generate a Gulf-wide baseline of polycyclic aromatic hydrocarbon (PAH) concentrations in demersal fishes. Tilefish (Lopholatilus chamaeleonticeps) were sampled in all regions of the Gulf of Mexico for biometrics, bile, and liver. Tilefish liver was also obtained from surveys in the northwest Atlantic Ocean for comparison. Liver tissues (n = 305) were analyzed for PAHs and select alkylated homologs using QuEChERS extractions and gas chromatography tandem mass spectrometry. Bile samples (n = 225) were analyzed for biliary PAH metabolites using high-performance liquid chromatography with fluorescence detection. Spatial comparisons indicate the highest levels of PAH exposure and hepatic accumulation in the north central Gulf of Mexico, with decreasing concentrations moving from the north central Gulf counterclockwise, and an increase on the Yucatán Shelf. Hepatic PAH concentrations were similar between the Gulf of Mexico and the northwest Atlantic, however, Tilefish from the northwest Atlantic had higher concentrations and more frequent detection of carcinogenic high molecular weight PAHs. Overall, results demonstrate that PAH pollution was ubiquitous within the study regions, with recent exposure and hepatic accumulation observed in Tilefish from both the Gulf of Mexico and northwest Atlantic.

The effects and mechanisms of biochars with different silicon (Si) contents on Cadmium (Cd) uptake, translocation and accumulation in rice plants are not fully understood. Herein, we report a pot study to disentangle the interaction mechanisms of Si-rich biochars (Sichar RH300, RH700) and Si-deficient biochars (WB300, WB700) with high-Si soil (HSS) and low-Si soil (LSS) on Cadmium (Cd) and Si accumulation in rice (including grains, straw, and roots). Sichar was found to be better than Si-deficient biochars in reducing Cd uptake and accumulation in rice, and RH300 amendment was better than the RH700 treatment. The surface complexation of Cd with carboxyl groups and Si from biochar led Cd immobilization in soil, as portrayed by Fourier transformed infrared spectroscopy and X-ray photoelectron spectroscopy. The high Si content of biochars indicates a relatively lower bioaccumulation factor and translocation factor of Cd. The Sichar (e.g., RH300) treatment significantly increases the silicon concentration in rice (including grains, straw, and roots), but the Si concentrations of rice grains and roots decrease with WB700-amended LSS. Negative correlations between the concentrations of rice Si and Cd were observed, which could be related to lower expression as observed by Si transport genes (Lsi1 and Lsi3) in rice by Sichar amendment. These findings suggest that the Si released from Sichars can reduce the gene expression of Si transport channel of rice roots and inhibit the transport channel of Si, thus thereby inhibiting the Cd uptake, probably due to the utilization of same channel for Cd and Si. Integrative mechanisms of Sichar (RH300 and RH700) reduced Cd plant accumulation can be proposed by soil immobilization, inhibition of root transport, and prevention of plant translocation.

Plastic marine debris hyper-concentrates hydrophobic contaminants such as polycyclic aromatic hydrocarbons (PAHs) and can transfer these sorbed contaminants to biota following ingestion. PAHs are known to induce cardiotoxicity and visual toxicity at sublethal doses. Juvenile White seabass (Atractoscion nobilis) fish were fed environmentally relevant concentrations of either virgin polystyrene or benzo(a)pyrene (BaP)-sorbed polystyrene for 5 days and were monitored for changes in phototactic response, swimming behavior, and hepatic cytochrome p450 1A (CYP1A) enzyme activity. No significant differences in the monitored endpoints were recorded in fish that ingested either polystyrene or BaP-sorbed polystyrene relative to control fish following the short-term exposure. However, fish exposed to 252 μg/L BaP alone as a positive control had significantly elevated CYP1A enzyme activity (p = 0.046) and impaired phototactic response (p = 0.020), though no altered swimming behavior was observed (p = 0.843) relative to control fish. These results demonstrate that pelagic fish ingesting environmentally relevant concentrations of BaP-sorbed polystyrene for a short, 5-day duration do not demonstrate measurable changes in vision, swimming activity, nor CYP1A activity. High variability within enzyme activity and behavioral responses suggest that lack of significant effects may be due to low sample size.