Arsenic (As) is among the most dangerous metalloids and is harmful to human wellbeing. In this laboratory study, Al(III)-modified kapok fibres (Al-Kapok) were used to remove As(V) from water. The sorbent was characterised using Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectroscopy (EDX). Batch experiments were performed to observe the performance of Al-Kapok in the removal of As(V) and to examine the effects of pH, temperature, adsorbent dose, and coexisting ions on the adsorption process. The surface of the sorbent changed after aluminium modification, and the results of the batch experiments showed that the adsorption of As(V) occurred mainly via endothermic-spontaneous chemisorption at the solution and solid interface of Al-Kapok. The As(V) removal efficiency was approximately 76%–84%, and it was slightly affected at pH levels below 8.0. Further study showed that the maximum adsorption capacity of Al-Kapok for As(V) was 118 μg/g at 30 °C and pH 6, and notable adverse effects were caused by the presence of SO42−and PO43−. It was also found that the boundary layer and film diffusion contributed more to As(V) adsorption. After five adsorption/desorption cycles, regeneration recovered approximately 92% of the adsorption capacity of Al-Kapok used. Overall, Al-Kapok appears to be a suitable adsorbent material for the purification of As-contaminated water.

Municipal sewage treatment plants (STPs) have been regarded as an important source of organic contaminants in aquatic environment. To assess the impact of STPs on occurrence and toxicity of STP-associated contaminants in receiving waterways, a novel passive sampler modified from polar organic chemical integrative sampler (m-POCIS) was deployed at the inlet and outlet of a STP and several upstream and downstream sites along a river receiving STP effluent in Guangzhou, China. Eighty-seven contaminants were analyzed in m-POCIS extracts, along with toxicity evaluation using zebrafish embryos. Polycyclic musks were the predominant contaminants in both STP and urban waterways, and antibiotics and current-use pesticides (e.g., neonicotinoids, fiproles) were also ubiquitous. The m-POCIS extracts from downstream sites caused significant deformity in embryos, yet the toxicity could not be explained by the measured contaminants, implying the presence of nontarget stressors. Sewage treatment process substantially reduced embryo deformity, chemical oxygen demand, and contamination levels of some contaminants; however, concentrations of neonicotinoids and fiproles increased after STP treatment, possibly due to the release of chemicals from perturbed sludge. Source identification showed that most of the contaminants found in urban waterways were originated from nonpoint runoff, while cosmetics factories and hospitals were likely point sources for musks and antibiotics, respectively. Although the observed embryo toxicity could not be well explained by target contaminants, the present study showed a promising future of using passive samplers to evaluate chemical occurrence and aquatic toxicity concurrently. Zebrafish embryo toxicity significantly decreased after sewage treatment, but higher toxicity was observed for downstream samples, demonstrating that urban runoff may produce detrimental effects to aquatic life, particularly in rainy season. These results highlight the relevance of monitoring nonpoint source pollution along with boosting municipal sewage treatment infrastructure.

In this study, we firstly used alginate to enhance an electrokinetic technology to remediate soil contaminated with divalent heavy metals (Pb²⁺, Cu²⁺, Zn²⁺). The mechanisms of alginate-associated migration of metal ions in electric field were confirmed. Alginate resulted in a high electrical current during electrokinetic process, and soil conductivity also increased after remediation. Obvious changes in both electroosmotic flow and soil pH were observed. Moreover, these factors were affected by increasing alginate dosage. The highest Cu (95.82%) and Zn (97.33%) removal efficiencies were obtained by introducing 1 wt% alginate. Alginate can desorb Cu²⁺ and Zn²⁺ ions from soil by forming unstable gels, which could be dissociated through electrolysis. However, Pb²⁺ ions did not easily migrate out of the contaminated soil. The density functional theory (DFT) calculations show Pb²⁺ ions could form a more stable coordination sphere in metal complexes than Cu²⁺ and Zn²⁺ ions. The metal removal efficiency was decreased by increasing alginate dosage at a high level. More alginate could provide more carboxyl ligands for divalent metal ions to stabilize gels, which were difficult to dissociate by electrolysis. In summary, the results indicate it is potential for introducing alginate into an electrokinetic system to remediate Cu- and Zn- contaminated soil.

The excessive proliferation of Microcystis aeruginosa can lead to ecological damage, economic losses, and threaten animal and human health. For controlling Microcystis blooms, microorganism-based methods have attracted much attention from researchers because of their eco-friendliness and species-specificity. Herein, we first found that a Paucibacter strain exhibits algicidal activity against M. aeruginosa and microcystin degradation capability. The algicidal activity of DH15 (2.1 × 10⁴ CFU/ml) against M. aeruginosa (2 × 10⁶ cells/ml) was 94.9% within 36 h of exposure. DH15 also degraded microcystin (1.6 mg/L) up to 62.5% after 72 h. We demonstrated that the algicidal activity of DH15 against M. aeruginosa can be mediated by physical attachment and indirect attack: (1) Both washed cells and cell-free supernatant could kill M. aeruginosa efficiently; (2) Treatment with DH15 cell-free supernatants caused oxidative stress, altered the fatty acid profile, and damaged photosynthetic system, carbohydrate, and protein metabolism in M. aeruginosa. The combination of direct and indirect attacks supported that strain DH15 exerts high algicidal activity against M. aeruginosa. The expression of most key genes responsible for photosynthesis, antioxidant activity, microcystin synthesis, and other metabolic pathways in M. aeruginosa was downregulated. Strain DH15, with its microcystin degradation capacity, can overcome the trade-off between controlling Microcystis blooms and increasing microcystin concentration. Our findings suggest that strain DH15 possesses great potential to control outbreaks of Microcystis blooms.

To evaluate the influence of surface coatings on nano-fertilizers uptake and their phytotoxicity to crops and its health risk to Chinese adults, trisodium citrate (TC) and polyethylene glycol (PEG) coatings were prepared on the surface of copper oxide nanoparticles (CuO NPs), respectively, with 100 and 500 mg/L of bare CuO NPs, TC-CuO NPs, and PEG-CuO NPs were exposed to soil-grown Ipomoea aquatica Forssk. Combined bio-transmission electron microscopy and micro-CT observed cellular migration of coated CuO NPs in symplastic and apoplastic pathways, as well as nanoparticles transported through vascular tissues to the above-ground parts. Since TC-CuO NPs had less inhibition on vascular phylogeny of I. aquatica roots which was determined by RT-qPCR, their migration in plants was more efficient, thus exhibiting greater phytotoxicity to shoots. Meanwhile, coatings significantly reduced the phytotoxicity of CuO NPs by stimulating plant antioxidant defense. The risk of CuO nano-fertilizers on human dietary safety was evaluated, the HQ > 1 in the 500 mg/L CuO NPs treatment indicated a potential health risk to Chinese adults, which was reduced by the coatings. This work explored for the first time the mechanism of coating effects on nanoparticles migration efficiency and phytotoxicity at the molecular level and demonstrated that the migration of nanoparticles between tissues could have an impact on phytotoxicity. It implied that coating can be tailored to target nanoparticles to specific regions of the plant. In addition, this study highlights the potential health risks associated with the consumption of I. aquatica fertilized with CuO NPs and provides valuable insights into the environmental applications of nano-fertilizers.

Morphological tools can assist in the evaluation of effects of insecticides on non-target insects. Pyriproxyfen, a juvenile hormone analog, is known to interfere with growth and metamorphosis of insects. However, there are studies showing indirect effects on natural enemies, including green lacewings. Few prior studies describe morphological effects of pyriproxyfen on target insect organs, especially on natural enemies. Through morphological tools, this study aimed to characterize the midgut and fat body, both important organs of digestion and great metabolic activity respectively, of the predator Ceraeochrysa claveri after chronic exposure to pyriproxyfen. Larvae of C. claveri were fed Diatraea saccharalis egg clusters treated with pyriproxyfen in solution of 50 or 100 mg a.i. L⁻¹ throughout the larval stage. The biological data revealed significant increases in development time, especially in the third instar, and in cumulative mortality from the prepupal into the pupal stage. Morphological analysis of adult midgut (≤24 h old) showed damage including formation of epithelial folds, intercellular spaces, emission of cytoplasmic protrusions. Both fat body regions presented decrease of lipid droplets, vacuolization of trophocytes and mitochondrial injury featuring a multisystemic action. In both organs, pyriproxyfen exposure induced significant oxidative stress by mitochondrial superoxide production. Cytoprotective responses were induced in midgut and fat body cells by augmenting the number of cytoplasmic granules containing calcium and expression of HSP 90. Both organs proved to be efficient in presenting histopathological alterations, showing the sensitivity and applicability of this morphological tool for evaluating other insecticides in non-target organisms.

Straw and biochar amendments have been shown to increase soil organic carbon (SOC) stocks in arable land; however, their effects on hydrological fluxes of dissolved organic carbon (DOC), which may offset the benefits of C sequestration amounts remain uncertain. Therefore, we conducted a three-year field study that included four treatments (CK, control with no fertilizer; NPK, synthetic N fertilizer; RSDNPK, synthetic N fertilizer plus crop residues; BCNPK, synthetic N fertilizer plus biochar of crop straw) to investigate the effects of straw and biochar amendment on DOC losses through hydrological pathways of overland flow and interflow from a wheat-maize rotation system in the subtropical montane agricultural landscape. We detected substantial intra- and inter-annual variations in runoff discharge, DOC concentration, and DOC fluxes for both overland flow and interflow pathways, which were primarily attributed to variations in rainfall amount and intensity. On average, the DOC concentrations for interflow (2.98 mg C L⁻¹) were comparable with those for overland flow (2.71 mg C L⁻¹) throughout the three-year experiment. However, average annual DOC fluxes for interflow were approximately 2.60 times greater than those for overland flow, which probably related to higher runoff discharges of interflow than overland flow. Compared to the control, on average, the N fertilization treatments significantly decreased the annual DOC fluxes of overland flow and significantly increased annual DOC fluxes of interflow. Relative to the application of synthetic N fertilizer only, on average, crop straw amendment practice significantly increased annual DOC fluxes of interflow by 28.7%, while decreasing annual DOC fluxes of overland flow by 12.0%; in contrast, biochar amendment practice decreased annual DOC fluxes of interflow by 25.3% while increasing annual DOC fluxes of overland flow by 44.6%. Overall, considering both overland flow and interflow, crop straw amendment significantly increased hydrological DOC fluxes, whereas biochar had no significant effects on hydrological DOC fluxes throughout the three-year experiment. We conclude that crop straw incorporation strategies that aim to increase SOC stocks may enhance hydrological losses of DOC, thereby in turn offsetting its benefits in the subtropical montane agricultural landscapes.

The emission factor (EF) is a parameter used to assess vehicle emissions. Many studies have reported EFs for vehicles in driving conditions. However, the idling emissions should not be neglected in characterizing actual vehicle emissions in congested large cities, where idling is very common on the road. Whereas, EF data for idling vehicles have scarcely been reported in the literature, let alone comparison of different fuels. In this study, the EFs of passenger cars burning four types of fuels - gasoline, compressed natural gas (CNG), diesel, and liquefied petroleum gas (LPG) were measured and compared. The emissions data for CO, CO₂, unburned hydrocarbon (HC), and NO were recorded to calculate fuel-based EFs in units of g pollutants/kg fuel burned. EFs for CO, HC, and NO were compared for the four fuels. Diesel vehicles had the highest EF for CO, with an average value of 35.12 ± 21.37 g/kg fuel, due to low concentration of CO₂ in lean operation compared to CO emission. CNG vehicles had the highest EF for HC, with an average value of 28.15 ± 11.97 g/kg fuel, due to high concentration of unburned methane gas due to slow CNG flame speed whereas diesel vehicles again had the highest EF for NO due to high temperature and pressure and freezing of NO decomposition reaction, with an average value of 12.07 ± 5.37 g/kg fuel. Further comparison was conducted to analyze the effects of two additional variables on EF: engine displacement volume and model/brand year. Only the gasoline-fueled vehicles showed an increase in EFs (for CO, HC and NO) with the vehicle age according to the model year. However, no clear correlation was observed for CNG, LPG, and diesel-fueled vehicles. Finally, the EF results were compared with those reported in the literature, which have been measured in various countries under both idling and non-idling conditions. Because the idling EFs were not substantially smaller than those under moving conditions, and vehicles spend substantial time idling in large cities, idling emissions should not be ignored in the emission inventories for large cities.

The bacteria toxicity of nanoscale zero-valent iron (nZVI) can be changed during its application in water treatment but the toxicity mechanism is still not well understood, particularly under anaerobic conditions. Here, the toxicity of nZVI and its aging products towards Escherichia coli (E. coli) and the mechanisms of extracellular and intracellular reactive oxygen species (ROS) damage were deeply probed in the presence and absence of oxygen in ultrapure water. Under aerobic conditions, the ROS damage primarily caused by the generation of extracellular free •OH can be a major contributor to the toxicity of nZVI to E. coli. By contrast, in anaerobic nZVI treatment system, the intracellular •OH can be quenched by benzoic acid which is a cell permeable quencher and the electron spin resonance (ESR) signals of 5,5-dimethy-1-pyrroline (DMPO)- •OH were evidently observed in system with the addition of F⁻ which could desorb the surface •OH into solution. It indicated that the intracellular •OH adsorbed on the particle surface can also play an indispensable role in inactivating cells under anaerobic conditions. Moreover, nZVI can steeply decline the membrane potential, causing severe membrane disruption and therefore resulting in the stronger toxicity in anaerobic conditions. Furthermore, the chemical composition transformation of nZVI and generation of benign iron corrosion products (e.g., Fe₃O₄, γ-Fe₂O₃, γ-FeOOH) are mainly responsible for the reduced toxicity with the increasing aging time. These results provide insights into the extracellular and intracellular ROS damage occurred in aerobic and anaerobic nZVI treatment systems, offering more perspective to the risk assessment of nZVI application.

The beta-blocker atenolol (ATE), and the selective serotonin and norepinephrine reuptake inhibitor, venlafaxine (VEN) are frequently detected in municipal wastewater effluents, but little is known about their ecotoxicological effect on aquatic animals. Herein, ATE and VEN were selected to explore their accumulation and global DNA methylation (GDM) in zebrafish tissues after a 30-day exposure. Molecular dynamics (MD) stimulation was used to investigate the toxic mechanism of ATE and VEN exposure. The results demonstrated that ATE and VEN could reduce the condition factor of zebrafish. The bioaccumulation capacity for ATE and VEN was in the order of liver > gut > gill > brain and liver > gut > brain > gill, respectively. After a 30-day recovery, ATE and VEN could still be detected in zebrafish tissues when exposure concentrations were ≥10 μg/L. Moreover, ATE and VEN induced global DNA hypomethylation in different tissues with a dose-dependent manner and their main target tissues were liver and brain. When the exposure concentrations of ATE and VEN were increased to 100 μg/L, the global DNA hypomethylation of liver and brain were reduced to 27% and 18%, respectively. In the same tissue exposed to the same concentration, DNA hypomethylation induced by VEN was more serious than that of ATE. After a 30-day recovery, the global DNA hypomethylations caused by the two drugs were still persistent, and the recovery of VEN was slower than that of ATE. The MD simulation results showed that both ATE and VEN could reduce the catalytic activity of DNA Methyltransferase 1 (DNMT1), while the effect of VEN on the 3D conformational changes of the DNMT1 domain was more significant, resulting in a lower DNA methylation rate. The current study shed new light on the toxic mechanism and potential adverse impacts of ATE and VEN on zebrafish, providing essential information to the further ecotoxicological risk assessment of these drugs in the aquatic environment.