The evaluation of DNA damage in aquatic organisms represents one of the most widely used biomarkers in the assessment and monitoring of marine pollution. Our previous research highlighted the presence of DNA damage in hepatic nuclei and blood cells of Coris julis specimens collected from Augusta harbor (Syracuse, Italy), a site highly polluted. In this work, we investigated on the ability of different natural compounds with antioxidant and immunostimulating properties, such as resveratrol (50 μM, 100 μM), amygdalin (100 μM, 200 μM), and Urtica dioica roots extract (50 and/or 100 μg/ml), in reducing DNA damage of C. julis. Blood cells were analyzed by atypical cellular comet assay. The results confirmed that Augusta specimens are the most damaged and showed that resveratrol, followed by amygdalin and U. dioica roots extract, drastically reduced DNA damage. This finding evidences the effectiveness of three natural compounds for DNA protection suggesting the possible use of feed enriched with antioxidant compounds in aquaculture practices for organisms damaged by natural and anthropic insults.

The cost effective maintenance of underwater pressure pipes for sewage disposal in Austria requires the detection and localization of leakages. Extrusion of wastewater in lakes can heavily influence the water and bathing quality of surrounding waters. The Distributed Temperature Sensing (DTS) technology is a widely used technique for oil and gas pipeline leakage detection. While in pipeline leakage detection, fiber optic cables are installed permanently at the outside or within the protective sheathing of the pipe; this paper aims at testing the feasibility of detecting leakages with temporary introduced fiber optic cable inside the pipe. The detection and localization were tested in a laboratory experiment. The intrusion of water from leakages into the pipe, producing a local temperature drop, served as indicator for leakages. Measurements were taken under varying measurement conditions, including the number of leakages as well as the positioning of the fiber optic cable. Experiments showed that leakages could be detected accurately with the proposed methodology, when measuring resolution, temperature gradient and measurement time were properly selected. Despite the successful application of DTS for leakage detection in this lab environment, challenges in real system applications may arise from temperature gradients within the pipe system over longer distances and the placement of the cable into the real pipe system.

This study proposes a method, backpropagation (BP) neural network, for interpolating missing values in daily precipitation time series. Firstly, the BP neural network is adopted to interpolate missing daily rainfall data at three selected stations in Yantai, Shandong, China. Then, the temporal and spatial variations in precipitation extremes across Shandong are analyzed by utilizing the complete daily rainfall dataset derived from accurate propagation at 24 meteorological stations. The results show that the long-term trends in five selected extreme precipitation indices calculated from interpolated daily rainfall data are generally consistent with those from original nonmissing values. And the spatial patterns of trends in precipitation extremes also show better performance for BP neural network approach in interpolating missing daily rainfall gaps. Those suggest that this BP neural network algorithm can obtain a good fit in terms of space-time variability of regional precipitation extremes, in case that the correlation coefficients between the target stations with missing values and reference stations with complete daily rainfall dataset are relatively large. These findings could be crucial for investigating regional frequency of heavy rainfall and water resource management.

Cities in developing countries encounter rapid waves of social transformation and economic development where the environment is mostly a neglected aspect. The Katari watershed encompasses mining areas, El Alto city (one of the fastest growing urban areas in South America and the biggest in the Altiplano) as well as agricultural areas. Its outlet is Cohana Bay, one of the most polluted areas of Lake Titicaca. Here we propose an integrative approach (hydrological, physicochemical, chemical and bacterial data) to understand the pollution problem of this developing area, in which a variety of anthropogenic activities takes place. Both mining and urban areas appear to be sources of metal pollution. Nutrient and bacterial contaminations are mainly related to urban and industrial discharges. These situations have impacts in the basin from the mining area down to Cohana Bay of Lake Titicaca. Pollutant concentration patterns are highly influenced by seasonal hydrology variations. The poor quality of surface waters in the basin represents a risk for human and animal populations, as well as for the quality of aquifers located underneath El Alto city.

The release of arsenic (As) from sediments poses a risk to human health especially at high pH levels. Despite this, the distribution and kinetic response of mobile As remains unclear under varying pH conditions. In this study, a microcosm incubation experiment was performed, using sediment cores in combination with dialysis (Peeper) and thin film diffusive gradients (DGT), to investigate the distributions of mobile As (soluble As in pore water and DGT-labile As) at high vertical resolutions (2–4 mm). Results show that the concentrations of soluble As present in the water column increased 1.5-fold with an increase in pH from 5.4 to 11.2. Both soluble As in pore water and DGT-labile As exhibited stable low-level distributions in the uppermost layer beneath the sediment-water interface, followed by increasing concentration distribution with decreasing layers to middle depths. The mean concentrations of mobile As species increased with increased water pH in both sediment profiles and with upward diffusion gradients, showing a 0.2-fold increase of soluble As in the top 20-mm layer and a 0.6-fold increase in deeper 20–52-mm layers, while DGT-labile As showed a 1.0- and 1.1-fold increase in these two layers, respectively. Modeling of DGT-induced flux in sediments (DIFS) showed that the desorption rate constant increased more rapidly than the absorption rate constant, resulting in the increased availability of solid As pools, therefore resupplying the soluble As in pore water from sediments.

Phosphate discharged in agricultural drainage causes water quality degradation on local, regional, and national scales. Iron oxyhydroxide filter materials can potentially remove the soluble phosphate present in drainage waters. Laboratory saturated column experiments and preliminary small-scale field tests were carried out to evaluate the effectiveness and efficiency of a synthetic goethite iron oxyhydroxide (α-FeOOH) filter material for phosphate treatment. Original iron oxyhydroxide filter material (SG-IOH-O) and the same filter material regenerated with sodium hydroxide (SG-IOH-R) were assessed. Results of replicated laboratory experiments showed that columns packed with SG-IOH-O or SG-IOH-R almost totally removed phosphate (>98%) from drainage waters spiked with an additional 1 or 10 ppm phosphate-P (PO₄-P). The column experiments with SG-IOH-O or SG-IOH-R additionally indicated that contact times of only 10 to 15 s were sufficient for near complete removal of phosphate from drainage water spiked with 1 ppm PO₄-P. In an initial small-scale filter treatment system field test with SG-IOH-O, percent phosphate removal averaged 89% in the first 200 days, which then decreased to an average 40% phosphate removal in the last 80 days. Following this initial field test, two field tests, one with SG-IOH-O and the other with SG-IOH-R, were conducted concurrently over a period of 193 days, with the SG-IOH-O system phosphate removal averaging 75%, while the SG-IOH-R system phosphate removal averaged 34%. This study’s findings support possible goethite iron oxyhydroxide filter material use for drainage water phosphate treatment; however, larger-scale field investigations are needed, particularly with modified regeneration procedures.

Understanding the sorption behavior of mixtures of pharmaceuticals is critical for predicting their environmental behavior and for risk assessment. Experiments on ketoprofen (KTP) and triclosan (TCS) sorption by wheat straw-derived biochars at 300 °C (WS300) and 700 °C (WS700) in single solute and bisolute systems were conducted to probe the sorption mechanisms. The results of the single solute sorption indicated that WS700 with higher degree of carbonization had higher sorption coefficient (K d) and nonlinearity than WS300. In a bisolute system, no competitive effect was observed for partition of neutral KTP and TCS in the noncarbonized phase of WS300, but they competed for the adsorptive sites on the carbonized phase of WS300 and WS700 for sorption, in which π-π interaction is proposed as the main mechanism. The competition in the bisolute system varied with degree of dissociation of KTP and TCS, and led to a lower and less nonlinear sorption compared with that in the single solute system. TCS was more competitive than KTP due to its higher hydrophobicity, and sorption inhibition of KTP was enhanced with increasing TCS concentration. Degree of both molecular dissociation and sorbent carbonization should be considered in bisolute sorption of organic pollutants by biochars.

An effective reagent for mercury desorption from contaminated soil is a key condition for mercury remediation. Effects of time, pH, temperature on mercury desorption using sodium sulfite were studied with a series of batch experiments. Results showed that desorption rate of mercury increased rapidly in the stage of 0 to 1 h, after that, a much slower stage appeared. Desorption rate reached 92.05% with 0.7 mol/L sodium sulfite at 25°C in 24 h. Moreover, potential value increased rapidly from −162 to –31 mV in desorption of 1 h. It indicates that desorption process was a process of Hg(II) turning into Hg(I). A higher pH (10.5) or temperature (35°C) was helpful to increase mercury desorption rate. Furthermore, small fold and curves appeared in the surface of soil particles presented by scanning electron microscopy (SEM) show that soil particles may be destroyed in desorption process using sodium sulfite. The desorption of Hg from contaminated soil was accomplished within a reductive solution provided by sodium sulfite.

The presence of fungicides in the natural environment, either resulting from deliberate actions or not, has become a serious threat to many ecosystems, including soil. This can be prevented by taking appropriate measures to clear the environment of organic contamination, including fungicides. Therefore, a study was conducted aimed at determining the effect of bioaugmentation of soil exposed to azoxystrobin on its degradation and activity of selected enzymes (dehydrogenases, catalase, urease, acidic phosphatase, alkaline phosphatase). A model experiment was conducted for 90 days on two types of soil: loamy sand (pHKCₗ—5.6) and sandy loam (pHKCₗ—7.0), which were contaminated by azoxystrobin at 22.50 mg kg⁻¹ DM of soil and inoculated with a specific consortium of microorganisms. Four strains of bacteria were used in the experiment (Bacillus sp. LM655314.1, B. cereus KC848897.1, B. weihenstephanensis KF831381.1, B. megaterium KJ843149.1) and two strains of mould fungi (Aphanoascus terreus AB861677.1, A. fulvescens JN943451.1). Inoculation of soil with the consortium of microorganisms accelerated the degradation of azoxystrobin. The isolated microorganisms were more active in loamy sand because within 90 days azoxystrobin was degraded by 24% (Bacillus sp., B. cereus, B. weihenstephanensis, B. megaterium) to 78% (Aphanoascus terreus, A. fulvescens). In sandy loam, azoxystrobin was degraded by 9% (Aphanoascus terreus, A. fulvescens) to 29% (Bacillus sp., B. cereus, B. weihenstephanensis, B. megaterium and Aphanoascus terreus, A. fulvescens). The activity of soil enzymes was also changed as a result of inoculation of soil with microorganisms. The activity of all of the enzymes under study was found to have increased when soil augmentation was performed.

The effect of styrene-divinylbenzene copolymer functionalization by carboxylic acid groups on the adsorption of p-nitrophenol (p-NP) from aqueous solutions was investigated. The adsorption capacity of p-NP onto the functionalized copolymer (CP-F) was compared with that of the precursor copolymer, the chloromethylated styrene-divinylbenzene copolymer (CP-N). The two copolymers were characterized by Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DSC-TG), specific surface area and particle size measurements, pore size distribution, scanning electron microscopy (SEM), and elemental analysis (EDX). The adsorption of p-NP was substantially enhanced after the polymer functionalization, and it was demonstrated that hydrogen bonding is principally responsible for the high adsorption capacity of CP-F in comparison with CP-N. The adsorption kinetics of p-NP adsorption onto CP-F was well described by the pseudo-second-order model. From the four investigated isotherms, Langmuir, Freundlich, Redlich-Peterson, and Sips, the equilibrium data were better described by the Sips model. The maximum adsorption capacity of the CP-F polymer resulting from the Sips isotherm was 243.37 mg g⁻¹. The capacity of regeneration and reuse of the CP-F polymer was evaluated in three consecutive cycles of adsorption-desorption.