Cyanobacterial blooms and their secondary metabolites, microcystins (MCs), are not only toxic to aquatic organisms, but also to humans. MCs exert reproductive toxicity in female fish by affecting the oocyte development. However, the mechanism behind MC-LR interference in oocyte development remains largely unknown. In our study, adult female zebrafish were exposed to MC-LR (0, 1, 5, 20 μg/L) for 30 d. After exposure to MC-LR for 30 d, fertilized eggs from the treated females and healthy males were collected and cultured in water without MC-LR. Histomorphological observations showed pathological damage in the ovary after MC-LR exposure, which was mainly characterized by enlarged intercellular spaces, detachment of follicular cells from oocytes, and vacuolation of parenchymal tissues. The 20 μg/L MC-LR treatment caused a remarkable increase in the rate of the zebrafish oocytes germinal vesicle breakdown (GVBD) and a significant decrease in the levels of cyclic adenosine monophosphate (cAMP) and vitellogenin (VTG). In addition, the phosphorylation levels of the extracellular signal-regulated kinases (ERK) were elevated in ovaries from zebrafish exposed to 5 and 20 μg/L MC-LR, and cyclinB phosphorylation levels were also upregulated notably in the 20 μg/L MC-LR group. However, MC-LR exposure did not cause any change in the levels of cAMP-dependent protein kinase (PKA) protein and cdc2 phosphorylation in all the treatments. All the doses of MC-LR reduced the number of eggs, prematurely hatched the fertilized eggs and increased the abnormal rate of offspring generation. In summary, the present study demonstrates that MC-LR promotes oocyte maturation by activating the ERK1/2 and MPF signaling pathways, and cAMP is involved in this process.

Addressing the presence of rutile nanoparticles (NPs) in the air is a work in progress, and the development of methodologies for the identification of NPs in atmospheric dust is essential for the assessment of its toxicological effects. To address this issue, we selected the fast growing desertic city of Hermosillo in northern Mexico. Road dust (n = 266) and soils (n = 10) were sampled and bulk Ti-contents were tested by portable X-ray fluorescence. NPs were extracted from atmospheric dust by PM₁.₀-PTFE filters and further characterized by Confocal Raman Microscopy, Energy-dispersive X-ray spectroscopy (EDS) coupled to Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). Results showed (i) the average concentration of Ti in road dust (3447 mg kg⁻¹) was similar to natural values and worldwide urban dusts; (ii) the bulk geochemistry was not satisfactory for Ti-NPs identification; (iii) 76% of the total extracted PM₁.₀ sample corresponded to NPs; (iv) mono-microaggregates of rutile NPs were identified; (v) ubiquitous polycyclic aromatic hydrocarbons (PAHs) were linked to NPs. The genotoxicity of rutile and PAHs, in connection with NPs content, make us aware of a crucial emerging environmental issue of significant health concern, justifying further research in this field.

To characterise the mass concentration, size-distribution, and respiratory deposition of selected trace metals (Cr, Mn, Fe, Ni, Cu, Zn, Ba, and Pb) in size-segregated PM₂.₅, a long-term monitoring campaign was undertaken at an urban background site in Como (Northern Italy). 96-h aerosol samples were collected weekly, from May 2015 to March 2016, using a 13-stage low pressure impactor and analysed via laser ablation-inductively coupled plasma-mass spectrometry. Significantly higher levels of trace metals were generally found during the heating season (two to more than four times) compared to the non-heating period at all size ranges, especially for concentrations in PM₀.₁–₁. Distinct distribution profiles characterised the different elements, even though the corresponding heating and non-heating shapes always exhibited similar features, with negligible seasonal shifts in the average mass median aerodynamic diameters. Fe, Ba, and Cu had >70% of their mass in PM₁–₂.₅, whereas Pb, Zn, and Ni showed higher contributions in the accumulation mode (>60%). Finally, broad size-distributions were found for Cr and Mn. The multiple-path particle dosimetry model estimated the overall deposition fractions in human airways varying between 27% (Pb) and 48% (Ba). The greatest deposition variability was always registered in the head region of the respiratory system, with the highest contributions for those metals predominantly accumulated in the PM₂.₅ coarse modes. In contrast, the deposition in the deepest respiratory tract maintained nearly constant proportions over time, becoming notably important for Pb, Ni, and Zn (∼13%) with respect to their total deposition. The comparison with national limits established for Pb and Ni suggested the absence of significant risks for the local population, as expected, with average concentrations two orders of magnitude lower than the corresponding annual limit and objective value. Similar findings were reported for all the other metals, for which the estimated hazard quotients were always well <1.

Score-based biotic indices are widely used to evaluate the water quality of streams and rivers. The main objective of this study was the assessmentof the water quality of Karoon River throught biotic indices based on the Family Biotic Index (HFBI), and the Biological Monitoring Working Party Score System (BMWP). Macroinveratabrate communities are one of the most used groups in assessment of water quality, since they respond directively to the level of contamination of aquatic ecosystems. Sampling were performed in 4 stations (Menikh, Zhian Canal, Bahrakani and Dahaneh). Sampling was carried in each station over one year ( winter, spring, summer and autumn). Macroinvertebrates were collected by the grab quantitative sampller. Four phylaum (Nematoda, Annelidae, Mollusca and Arthropoda) distributed amoung 11 families were sampled. Station1 (Menikh) had the highest Macroinveratabrate diversity and richness. The highest density of aquatic invertabrates were recorded in winter (Annelidae 46%, Crustacea 28% ,Mullusca 21% and Diptera 5% ) and spring ( Mullusca 58%, Crustacea 27%,Nematoda 10% and Anellidae 5%) respectively. The BMWP index showed that the water quality was in the reguallar category (for Menikh, Zhian Canal and Bahrakani) and in the poor category (for Dahaneh). HFBI index pointed out the water quality was in the reguallar catagorry (for Menikh, Zhian Canal and Bahrakani) and poor category (for Dahaneh) . Biotic indices used reflected the changes to the water quality.

Over the past few decades, human societies have faced one of the most significant consequences of industrial development. That is the improper transportation and disposal of hazardous and industrial-specific waste, which accounts for a large share of the total pollution of the environment, and its destructive effects on creating environmental crises are quite evident. The plating industry is one of the main consumers of toxic chemicals used for different applications. However, because of the lack of a comprehensive model, hazardous waste management has not yet met the current needs, and its principles still require more development. This study was carried out using tools and standards including Multi-Criteria Decision Support Systems (MCDSS) and Fuzzy Analytical Hierarchy Process (FAHP) to rank the contributions of effective components to the plating industry in a case study conducted and implemented in Paitakht Industrial District of Tehran. In order to realize this objective, after extracting 10 components effective in optimizing the management of industrial wastes, questionnaires were used to survey experts to verify the components. The factors were identified using the Delphi method through the analysis of frequency carried out using a second questionnaire based on the results of the analysis of the data extracted from the first questionnaire. The results showed that components contributing to the optimal management of industrial waste included leadership and management, policy, strategy, technology and mode of collection and transportation, economic factors, policy making, expertise, culture, education, and the amount and composition of waste, in that order.

The purpose of this study is to identify environmental pollution caused by tourism activities in Shahrood county and environmental risk assessment. In the first step, tourism sites (recreational-sports) in the study area were identified. In the next step, the type and extent of environmental pollution in each were identified and their zoning map was drawn. Finally, risk assessment was performed using risk assessment models. The results showed that some types of environmental pollution can be seen in these areas, but the pollution caused by solid waste has been present in all tourist sites. The daily sewage pollution rate produced by natural tourism in Shahrood is relatively high and is about 140kg/day and the waste production rate is 10 tons per year. Also; the average measured sound level is 88dB. In terms of the level of risk of pollutants, the three most common types of pollution are solid waste and then sewage. The lowest type of pollution is related to air pollution. As for air pollution, the type of risk is obvious and the level of the aspect is low. In the case of noise pollution, the type of risk is obvious and the level of risk is high. In the case of wastewater pollution and waste pollution, the type of risk, the level and the level of the aspect is the ultimate. The principles of sustainable tourism in this region have not been observed and the need to develop a comprehensive and strategic model for tourism management is essential.

Organisms are exposed to various stressors including parasites and micropollutants. Their combined effects are hard to predict. This study assessed the trophic relationship, micropollutants bioaccumulation and infection degree in a host-parasite couple. Carbon and nitrogen isotopic ratios were determined in hake Merluccius merluccius muscle and in its parasite Anisakis sp.. Concentrations of both priority (mercury species and polychlorinated biphenyls congeners) and emerging (musks and sunscreens) micropollutants were also measured for the parasite and its host, to detect potential transfer of contaminants between the two species. The results showed partial trophic interaction between the parasite and its host, in accordance with the Anisakis sp. life encysted in hake viscera cavity. PCB transfer between the two species may result from some lipids uptake by the parasite, while no relation occurred for the two other contaminants. Finally, a positive correlation was found between the number of Anisakis sp. larvae and the methylmercury contamination for hake, emphasizing the assumption that the contamination level in methylmercury can weaken immune system of the host enough to affect parasite infection degree.

A combined approach integrating bioenergetics and major biological activities is essential to properly understand the impact of microplastics (MP) on marine organisms. Following experimental exposure of polystyrene microbeads (micro-PS of 6 and 10 μm) at 0.25, 2.5, and 25 μg L−1, which demonstrated a dose-dependent decrease of energy balance in the pearl oyster Pinctada margaritifera, a transcriptomic study was conducted on mantle tissue. Transcriptomic data helped us to decipher the molecular mechanisms involved in P. margaritifera responses to micro-PS and search more broadly for effects on energetically expensive maintenance functions. Genes related to the detoxification process were impacted by long-term micro-PS exposure through a decrease in antioxidant response functioning, most likely leading to oxidative stress and damage, especially at higher micro-PS doses. The immune response was also found to be dose-specific, with a stress-related activity stimulated by the lowest dose present after a 2-month exposure period. This stress response was not observed following exposure to higher doses, reflecting an energy-limited capacity of pearl oysters to cope with prolonged stress and a dramatic shift to adjust to pessimum conditions, mostly limited and hampered by a lowered energetic budget. This preliminary experiment lays the foundation for exploring pathways and gene expression in P. margaritifera, and marine mollusks in general, under MP exposure. We also propose a conceptual framework to properly assess realistic MP effects on organisms and population resilience in future investigations.