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Responses of the reproduction, population growth and metabolome of the marine rotifer Brachionus plicatilis to tributyl phosphate (TnBP) 全文
2021
Zhang, Xin | Tang, Xuexi | Yang, Yingying | Sun, Zijie | Ma, Wenqian | Tong, Xin | Wang, Chengmin | Zhang, Xinxin
The typical alkyl organophosphorus flame retardant tributyl phosphate (TnBP) can leak from common products into the marine environment, with potential negative effects on marine organisms. However, risk assessments for TnBP regarding zooplankton are lacking. In this study, a marine rotifer, Brachionus plicatilis, was used to analyze the effect of TnBP (0.1 μg/L, environmental concentration; 1 and 6 mg/L) on reproduction, population growth, oxidative stress, mitochondrial function and metabolomics. Mortality increased as the TnBP concentration rose; the 24-h LC₅₀ value was 12.45 mg/L. All tested TnBP concentrations inhibited B. plicatilis population growth, with reproductive toxicity at the higher levels. Microstructural imaging showed ovary injury, the direct cause of reproductive toxicity. Despite elevated glutathione reductase activities, levels of reactive oxygen species and malonyldialdehyde increased under TnBP stress, indicating oxidative imbalance. TnBP induced mitochondrial malformation and activity suppression; the ROS scavenger N-acetylcysteine alleviated this inhibition, suggesting an internal connection. Nontargeted metabolomics revealed 398 and 583 differentially expressed metabolites in the 0.1 μg/L and 6 mg/L treatments relative to control, respectively, which were enriched in the pathways such as biosynthesis of amino acids, purine metabolism, aminoacyl-tRNA biosynthesis. According to metabolic pathway analysis, oxidative stress from purine degradation, mitochondrial dysfunction, disturbed lipid metabolism and elevated protein synthesis were jointly responsible for reproduction and population growth changes. This study echoes the results previously found in rotifer on trade-off among different life processes in response to environmental stress. Our systematic study uncovers the TnBP toxic mode of action.
显示更多 [+] 显示较少 [-]Bioaccumulation potential of chlorpyrifos in resistant Hyalella azteca: Implications for evolutionary toxicology 全文
2021
Johanif, Nadhirah | Huff Hartz, Kara E. | Figueroa, Alexandra E. | Weston, Donald P. | Lee, Devon | Lydy, Michael J. | Connon, Richard E. | Poynton, Helen C.
Given extensive use of pesticides in agriculture, there is concern for unintended consequences to non-target species. The non-target freshwater amphipod, Hyalella azteca has been found to show resistance to the organophosphate (OP) pesticide, chlorpyrifos, resulting from an amino acid substitution in acetylcholinesterase (AChE), suggesting a selective pressure of unintended pesticide exposure. Since resistant organisms can survive in contaminated habitats, there is potential for them to accumulate higher concentrations of insecticides, increasing the risk for trophic transfer. In the present study, we estimated the uptake and elimination of chlorpyrifos in non-resistant US Lab, and resistant Ulatis Creek (ULC Resistant), H. azteca populations by conducting 24-h uptake and 48-h elimination toxicokinetic experiments with ¹⁴C-chlorpyrifos. Our results indicated that non-resistant H. azteca had a larger uptake clearance coefficient (1467 mL g⁻¹ h⁻¹) than resistant animals (557 mL g⁻¹ h⁻¹). The half-life derived from the toxicokinetic models also estimated that steady state conditions were reached at 13.5 and 32.5 h for US Lab and ULC, respectively. Bioaccumulation was compared between non-resistant and resistant H. azteca by exposing animals to six different environmentally relevant concentrations for 28 h. Detection of chlorpyrifos in animal tissues indicated that resistant animals exposed to high concentrations of chlorpyrifos were capable of accumulating the insecticide up to 10-fold higher compared to non-resistant animals. Metabolite analysis from the 28-h concentration experiments showed that between 20 and 50 % parent compound was detected in H. azteca. These results imply that bioaccumulation potential can be more significant in chlorpyrifos resistant H. azteca and may be an essential factor in assessing the full impacts of toxicants on critical food webs, especially in the face of increasing pesticide and chemical runoff.
显示更多 [+] 显示较少 [-]Structural and functional analysis of the inhibition of equine glutathione transferase A3-3 by organotin endocrine disrupting pollutants 全文
2021
Škerlová, Jana | Ismail, Aram | Lindström, Helena | Sjödin, Birgitta | Mannervik, Bengt | Stenmark, Pål
Organotin compounds are highly toxic environmental pollutants with neurotoxic and endocrine-disrupting effects. They are potent inhibitors of glutathione transferases (GSTs), thus impeding their detoxication and antioxidant functions. Several GSTs, including equine GST A3-3 (EcaGST A3-3), exhibit steroid double-bond isomerase activity and are involved in the biosynthesis of testosterone and progesterone. We have performed enzyme kinetics analyses of the inhibition of EcaGST A3-3 by organotin compounds. We have also solved crystal structures of EcaGST A3-3 in complexes with glutathione, and with glutathione together with covalently bound triethyltin. Our structural data indicate that the tin atom forms strong bonds with a covalent character not only with the glutathione, but also with a tyrosyl residue of the enzyme itself, thereby preventing the release of the glutathione-organotin adduct and completely blocking the enzyme function. This work presents a structural basis for the general mechanism of GST inhibition by organotin compounds and contributes to the understanding of their neurotoxic and endocrine disrupting effects.
显示更多 [+] 显示较少 [-]Mitigation of global warming potential and greenhouse gas intensity in arable soil with green manure as source of nitrogen 全文
2021
Lee, Hyun Ho | Kim, Sung Un | Han, Hae Ri | Hur, Do Yeong | Owens, Vance N. | Kumar, Sandeep | Hong, Chang Oh
This study was conducted to determine the effect of different green manure treatments on net GWP and GHGI in upland soil. Barley (B), hairy vetch (HV), and a barley/hairy vetch mixture (BHV) were sown on an upland soil on November 4, 2017 and October 24, 2018. The aboveground biomass of these green manures was incorporated into soil on June 1, 2018 and May 8, 2019. In addition, a fallow treatment (F) was installed as the control. Maize was transplanted as the subsequent crop after incorporation of green manures. Green manuring significantly affected CO₂ and N₂O emission, but not CH₄. Average cumulative soil respiration across years with HV and BHV were 37.0 Mg CO₂ ha⁻¹ yr⁻¹ and 35.8 Mg CO₂ ha⁻¹ yr⁻¹, respectively and significantly higher than those with under F and B (32.7 Mg CO₂ ha⁻¹ yr⁻¹ and 33.0 Mg CO₂ ha⁻¹ yr⁻¹, respectively). Cumulative N₂O emissions across years with F and HV were 6.29 kg N₂O ha⁻¹ yr⁻¹ and 5.44 kg N₂O ha⁻¹ yr⁻¹, respectively and significantly higher than those with B and BHV (4.26 kg N₂O ha⁻¹ yr⁻¹ and 4.42 kg N₂O ha⁻¹ yr⁻¹, respectively). The net ecosystem carbon budget for HV (−0.5 Mg C ha⁻¹ yr⁻¹) was the greatest among the treatments (F; −1.61 Mg C ha⁻¹ yr⁻¹, B; −3.98 Mg C ha⁻¹ yr⁻¹, and BHV; −0.91 Mg C ha⁻¹ yr⁻¹) because of its high biomass yields and the yield of maize after incorporation of HV. There was no significant difference of GHGI among F, HV, and BHV. Incorporation of HV or BHV could reduce net CO₂ emissions per unit of maize grain production as well as F.
显示更多 [+] 显示较少 [-]How fast, how far: Diversification and adoption of novel methods in aquatic microplastic monitoring 全文
2021
Rist, Sinja | Hartmann, Nanna B. | Welden, Natalie A.C.
Since 2004, there has been a marked diversification in the methods used to determine aquatic microplastic (MP) concentrations. Despite calls for a unified approach to MP sampling, the proliferation of new methods has accelerated in recent years. Both minor method adaptations and entirely novel approaches have been introduced to overcome barriers to reliable MP sampling, extraction and quantification, resulting in a variety of complimentary but also competing approaches. However, there is little clarity regarding the extent to which new methods are acknowledged and adopted, or of the apparent drivers of, as well as barriers to, said adoption. To explore these issues, the rate of method diversification was examined in a systematic review. The rate and degree of diversification were determined by scoring each method by its “degree of novelty”: highly novel methods, secondary adaptations of existing methods and smaller, tertiary adaptations of existing methods. This analysis revealed that the rate of method diversification has been greatest since 2011. Our results indicate limited use of these novel methods and adaptations in the subsequent literature, with many researchers falling back on methods that are well established in the existing literature. Importantly, there is little consistency in the units used when reporting MP concentrations. However, these differences are seldom driven by method selection and are rather the result of discrepancies between researchers. Thus, in understanding the requirements of comparability and consistent reporting for monitoring purposes, we can apply a diverse approach to sampling whilst maintaining the applicability and usefulness of the resulting data.
显示更多 [+] 显示较少 [-]Ambient particulate matter triggers defective autophagy and hijacks endothelial cell renewal through oxidative stress-independent lysosomal impairment 全文
2021
Wang, Yan | Ma, Ying | Yao, Yongshuai | Liu, Qing | Pang, Yanting | Tang, Meng
Ambient particulate matter (APM) has been authenticated to exert hazards on human vascular endothelial cells, including abnormal autophagy. However, the potential reasons for autophagosome accumulation are still obscure. Since autophagy is a dynamic process, it is imperative to systemically consider the autophagic induction combined with its degradation to reflect realistic scenarios. Therefore, in the current study, different exposure durations were initially employed for the detection of autophagic marker proteins to assess the dynamic autophagic state preliminarily. Additionally, LC3 turn-over and autophagic flux assays were used to determine the specific cause of LC3II upregulation in EA.hy926 human vascular endothelial cells by a type of standard urban particulate matter, PM SRM1648a. As a result, PM SRM1648a stimulates excess autophagic vacuoles in EA. hy926 cells, in which the underlying causes are probably different at varying incubation endpoints. Intriguingly, LC3II upregulation was due to the intensifying autophagic initiation after 6 h of exposure, whereas as exposure period was extended to 24 h, overloaded autophagic vacuoles were attributed to the defective autophagy. Mechanistically, PM SRM1648a damages EA. hy926 cells by inducing lysosomal disequilibrium and resultant autophagic malfunction which are not directly mediated by oxidative stress. These data indicate that appropriate maintenance of lysosomal function and autophagic flux is probably a protective measure against APM-induced endothelial cell damage.
显示更多 [+] 显示较少 [-]Homogenization of diatom assemblages is driven by eutrophication in tropical reservoirs 全文
2021
Zorzal-Almeida, Stéfano | Bartozek, Elaine C Rodrigues | Bicudo, Denise C.
Eutrophication is one of the most widespread causes of biotic homogenization in freshwater ecosystems. Biotic homogenization can be characterized as reductions in local diversity (alpha) and occupation of available niches by more generalist species. Beta diversity is expected to decrease in more homogeneous communities, however, there is no consensus on how it responds to eutrophication. We used a space-for-time approach to analyze the process of biotic homogenization on diatom assemblages in response to eutrophication in tropical reservoirs ranging from oligotrophic to hypereutrophic conditions. Diatom assemblages were analyzed in phytoplankton and surface sediment from 12 reservoirs with different trophic levels. We calculated total beta diversity and turnover and nestedness components and used regressions to analyze their relationships with productivity differences (without distance effects). Total beta diversity had a positive influence of the trophic gradient, whereas turnover was not related to eutrophication. However, we found that eutrophication and lower species richness (alpha diversity) led to increasing rates of the nestedness component. We also observed that the homogenization process was not characterized by invasion of new species, but, on the contrary, by filtering nutrient-rich tolerant species also present in oligo-mesotrophic reservoirs and able to occupy available niches in the eutrophic reservoirs. These findings (increase in nestedness, decrease in alpha diversity, and development of tolerant species) suggest that biotic homogenization is leading to a simplification of diatom assemblages in tropical reservoirs, making assemblages from eutrophic and hypereutrophic reservoirs a subset of assemblages from oligotrophic and mesotrophic ones.
显示更多 [+] 显示较少 [-]Nationwide monitoring of microplastics in bivalves from the coastal environment of Korea 全文
2021
Cho, Youna | Shim, Won Joon | Jang, Mi | Han, Gi Myung | Hong, Sang Hee
Bivalves are useful bioindicators of microplastic contamination in the marine environment for several reasons, such as extensive filter feeding activity, broad geographical distribution, and limited movement capability. This study conducted a nationwide monitoring of microplastic pollution along the Korean coasts using filter-feeding bivalves (including oyster, mussel, and Manila clam) as bioindicators to identify the national contamination level and characteristics of microplastics. Seawater sample was collected from the same sampling stations of oyster and mussel for comparison. Microplastics were widely distributed in both coastal bivalves and waters with mean concentrations of 0.33 ± 0.23 n/g (1.21 ± 0.68 n/individual) in oyster/mussel, 0.43 ± 0.32 n/g (2.19 ± 1.20 n/individual) in Manila clam, and 1400 ± 560 n/m³ in seawater. Despite the lack of significant relationship in the abundance of microplastics, their dominant features such as size, shape, color and polymer type were similar between bivalves and seawater. Fragments (69% for oyster/mussel, 72% for Manila clam, and 77% for seawater), particles smaller than 300 μm (96% for oyster/mussel, 83% for Manila clam, and 84% for seawater) and colorless (79% for oyster/mussel, 85% for Manila clam, 75% for seawater) were the dominant shape, size and color, respectively. The major polymer types were polypropylene, polyethylene, and polyester. The microplastic level in bivalves was relatively high in urbanized areas with a wide diversity of polymer types compared with those in non-urbanized areas, and the proportion of polystyrene in the Korean samples was abundant compared with other regions due to wide use of polystyrene products in Korea. Our result suggests that microplastic contamination is widespread in the Korean coastal environment, and bivalves can reflect the microplastic pollution characteristics of the surrounding waters where they live.
显示更多 [+] 显示较少 [-]Vaporization characteristics and aerosol optical properties of electronic cigarettes 全文
2021
Wu, Jinlu | Yang, Muyun | Huang, Jiejie | Gao, Yihan | Li, Dian | Gao, Naiping
The aerosols generated from electronic cigarettes have a significant impact on the human respiratory system. Understanding the vaporization characteristics and aerosol optical properties of electronic cigarettes is important for assessing human exposure to aerosols. An experimental platform was designed and built to simulate the atomization process of electronic cigarette and detect the laser transmissivity of aerosols. The optical properties of single particles and polydispersed particle system for aerosols in the visible wavelength ranges of 400–780 nm were analyzed based on Mie theory. The results show that a higher heating power supplied by coil results in a larger average vaporization rate of e-liquid. Meanwhile, the steady-state transmissivity of the laser beam for aerosols reduces as the heating power increases. Under the same heating power and puffing topography, the total particulate mass (TPM) of aerosols generated by the e-liquid composed of higher vegetable glycerin (VG) content decreases. The scattering efficiency factor of aerosol particle of electronic cigarette increases with an increase in particle size. The volume scattering coefficients of a polydispersed particle system of aerosols decrease as the incident visible wavelengths increase. A higher VG content in e-liquid results in decreased TPM and particle number concentration of aerosols and increased the volume scattering coefficient in the visible wavelength range. It can explain an interesting phenomenon that a lower TPM and a better visual effect brought by the aerosols generated by the e-liquid with a higher VG content could be observed concurrently. The mass indexes (e.g., TPM, average vaporization rate, average mass concentration) and optical indexes (e.g., volume scattering coefficient, laser transmissivity) are suggested to be used for the comprehensive evaluation of relative amounts of aerosols. The results have potential significances for the objective and quantitative assessments of aerosols generated from electronic cigarettes.
显示更多 [+] 显示较少 [-]PM1 chemical composition and light absorption properties in urban and rural areas within Sichuan Basin, southwest China 全文
2021
Zhao, Suping | Yin, Daiying | Yu, Ye | Kang, Shichang | Ren, Xiaolin | Zhang, Jing | Zou, Yong | Qin, Dahe
Sichuan Basin is encircled by high mountains and plateaus with the heights ranging from 1 km to 3 km, and is one of the most polluted regions in China. However, the dominant chemical species and light absorption properties of aerosol particles is still not clear in rural areas. Chemical composition in PM₁ (airborne particulate matter with an aerodynamic diameter less than 1 μm) and light-absorbing properties were determined in Chengdu (urban) and Sanbacun (rural) in western Sichuan Basin (WSB), Southwest China. Carbonaceous aerosols and secondary inorganic ions (NH₄⁺, NO₃⁻ and SO₄²⁻) dominate PM₁ pollution, contributing more than 85% to PM₁ mass at WSB. The mean concentrations of organic and elemental carbon (OC, EC), K⁺ and Cl⁻ are 19.69 μg m⁻³, 8.00 μg m⁻³, 1.32 μg m⁻³, 1.16 μg m⁻³ at the rural site, which are 26.2%, 65.3%, 34.7% and 48.7% higher than those at the urban site, respectively. BrC (brown carbon) light absorption coefficient at 405 nm is 63.90 ± 27.81 M m⁻¹ at the rural site, contributing more than half of total absorption, which is about five times higher than that at urban site (10.43 ± 4.74 M m⁻¹). Compared with secondary OC, rural BrC light absorption more depends on primary OC from biomass and coal burning. The rural MAEBᵣC (BrC mass absorption efficiency) at 405 nm ranges from 0.6 to 5.1 m² g⁻¹ with mean value of 3.5 ± 0.8 m² g⁻¹, which is about three times higher than the urban site.
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