After 30 years of development of constructed wetlands (CWs) treating raw domestic wastewater for small communities (< 5,000 people equivalent, pe) in France, about 3,500 plants are currently in operation. If most of them are composed of two vertical stages in series, many configurations exist (association with horizontal flow or pond systems, vertical flow with recirculation, partially saturated systems, etc.). A previous database analysis was carried out 10 years ago on the classical French system allowing the global performances to be observed. Nowadays, the database improvement, thanks to Irstea and the monitoring of the French technical department on full-scale two vertical stages (VFCW), allows improving our understanding and having a greater perspective on the treatment performances of these configurations fed with raw wastewater. Typical concentrations at the second stage outlet are 74mgCOD.L -1 , 17 mgTSS.L -1 and 11 mgTKN.L -1. This paper presents: -A description of existing VFCW based on the study of 415 plants;-An investigation of pollutant removal performances in relation to the loads applied for the first treatment stage.

French vertical flow constructed wetlands (VFCW) treating raw wastewater have been developed successfully over the last 30 years. The highly aerobic treatment observed on these two-stages of filter allows an almost complete nitrification and accepts high hydraulic loads with seasonal variations. Nevertheless, the French two-stage VFCWs need a total filtration area of 2-2.5 m2/PE and the use of sand on the second stage. Furthermore, it is not always economically competitive and their high removal efficiencies are not systematically needed. In this context, implementing a one-stage system allowing reaching standard requirements is of interest. One of the solutions developed in France is to use a vertical flow system with an unsaturated layer at the top and a saturated layer at the bottom. It is proposed by Epur Nature as Biho-Filter®. The aim of this study was to assess the potential of this new configuration, in order to optimize the design and operating conditions (bottom saturation layer depth, recirculation rate, etc.). The study consisted in hydraulic and removal assessment of 3 different Biho-Filter® plants. It presents the results regarding outflow concentrations that can be achieved (15 mg.L -1 in TSS, 70 mg.L -1 in COD, 15 mg.L -1 in BOD5, 25 mg.L -1 in TKN and 70% in TN removal) as well as the impact of the operation conditions on treatment performances and the limits of the systems.

Buffer zones between wastewater treatment plants and receiving water bodies have recently gained interest in France. These Planted Discharge Areas (PDAs) receive treated wastewater, and may have various designs aiming to mimic “natural” kinds of wetlands. Research is needed to assess the treatment efficiency of such systems: a comprehensive study is carried out to understand the fate of water, conventional pollutants (suspended solids, organic carbon, ammonium, and phosphates), micro pollutants that are refractory to up-stream biological treatment, and pathogens. Special attention must be paid to understand the fate of the infiltrated treated wastewater in the field where PDAs are built, in order to ensure their long-term operation (system scale) and to protect the underground water bodies (site scale). To address these issues, the authors propose a comprehensive strategy combining successive stages using either geological or hydrological methods. These techniques provide the following prominent information for a proper design of PDAs: (1) the number and the location of the different soil layers; (2) the infiltration capacity of each layers; (3) the water table (height of the groundwater). Furthermore, the seasonal variation (if any) of the land characteristics are also determined. The proposed strategy successfully determined the fate of the infiltrated treated wastewater on the land considered before the implementation of the semi-industrial scale PDAs in Bordeaux. Besides, methods used for long-term PDA efficiency assessment are presented.

This study set out to assess how vertical flow constructed wetlands (vfCW) adapt to different types of tourism-driven variations in influent load, i.e. (i) campsites, closed for six months of the year and featuring a two-month-only window of high-season activity, and (ii) tourist-interest villages either hosting tourists over at least the six months of summer while the permanent population is resident or summer festivals that create intense pollution loads in a short burst of just a few days. The study surveyed 4 campsites and 4 tourist-interest villages that were monitored for several years, generating over 70 performance balances for vfCW that were intentionally scaled down in relation to the conventional French design for experimental trials. The influent wastewater effectively qualifies as domestic sewage, although relatively concentrated, with the campsites presenting particularly high nitrogen concentrations (122 gTKN.L-1). The applied daily loads were also particularly high, with some combinations of load parameters (hydraulic load, organic matter, TKN) leading to 400% overloading. Even under these drastic conditions, quality of effluent remained excellent on the characteristic organic matter parameters, with removal performances always over 85%. However, nitrification performances were poor to good. Analysis of the dataset points to two major design thresholds: For campsites, in order to keep a 73% nitrification rate even at the height of the summer season, the load applied to the 1st stage filter in operation has to be capped at less than 600 gCOD.m-².d-1. For tourist-interest villages, in order to keep an 85% nitrification rate in the summer season, the load applied to the 2nd stage filter in operation has to be capped at less than 22 gTKN.m-².d-1. Here, vfCW were demonstrated to robustly handle a massive increase in loads applied, providing the wetland construction and operation stringently follow best design standards and practices.

Composting has been demonstrated to be effective in degrading organic pollutants (OP) whose behaviour depends on the composting conditions, the microbial populations activated and interactions with organic matters. The fate of OP during composting involves complex mechanisms and models can be helpful tools for educational and scientific purposes, as well as for industrialists who want to optimise the composting process for OP elimination. A COP-Compost model, which couples an organic carbon (OC) module and an organic pollutant (OP) module and which simulates the changes of organic matter, organic pollutants and the microbial activities during the composting process, has been proposed and calibrated for a first set of OP in a previous study. The objectives of the present work were (1) to introduce the COP-Compost model from its convenient interface to a potential panel of users, (2) to show the variety of OP that could be simulated, including the possibility of choosing between degradation through co-metabolism or specific metabolism and (3) to show the effect of the initial characteristics of organic matter quality and its microbial biomass on the simulated results of the OP dynamic. In the model, we assumed that the pollutants can be adsorbed on organic matter according to the biochemical quality of the OC and that the microorganisms can degrade the pollutants at the same time as they degrade OC (by co-metabolism). A composting experiment describing two different 14C-labelled organic pollutants, simazine and pyrene, were chosen from the literature because the four OP fractions simulated in the model were measured during the study (the mineralised, soluble, sorbed and non-extractable fractions). Except for the mineralised fraction of simazine, a good agreement was achieved between the simulated and experimental results describing the evolution of the different organic fractions. For simazine, a specific biomass had to be added. To assess the relative importance of organic matter dynamics on the organic pollutants’ behaviour, a sensitivity analysis was conducted. The sensitivity analysis demonstrated that the parameters associated with organic matter dynamics and its initial microbial biomass greatly influenced the evolution of all the OP fractions, although the initial biochemical quality of the OC did not have a significant impact on the OP evolution

International audience | The remediation of copper-contaminated soils by aided phytostabilisation in 16 field plots at a wood preservation site was investigated. The mobility and bioavailability of four potentially toxic trace elements (PTTE), i.e., Cu, Zn, Cr, and As, were investigated in these soils 4 years after the incorporation of compost (OM, 5 % w/w) and dolomite limestone (DL, 0.2 % w/w), singly and in combination (OMDL), and the transplantation of mycorrhizal poplar and willows. Topsoil samples were collected in all field plots and potted in the laboratory. Total PTTE concentrations were determined in soil pore water (SPW) collected by Rhizon soil moisture samplers. Soil exposure intensity was assessed by Chelex100-DGT (diffusive gradient in thin films) probes. The PTTE phytoavailability was characterized by growing dwarf beans on potted soils and analyzing their foliar PTTE concentrations. OM and DL, singly and in combination (OMDL), were effective to decrease foliar Cu, Cr, Zn, and As concentrations of beans, the lowest values being numerically for the OM plants. The soil treatments did not reduce the Cu and Zn mineral masses of the bean primary leaves, but those of Cr and As decreased for the OM and DL plants. The Cu concentration in SPW was increased in the OM soil and remained unchanged in the DL and OMDL soils. The available Cu measured by DGT used to assess the soil exposure intensity correlated with the foliar Cu concentration. The Zn concentrations in SPW were reduced in the DL soil. All amendments increased As in the SPW. Based on DGT data, Cu availability was reduced in both OM and OMDL soils, while DL was the most effective to decrease soil Zn availability.

[Departement_IRSTEA]Eaux [TR1_IRSTEA]ARCEAU | International audience | Pesticide pollution is one of the main current threats on water quality. This paper presents the potential and functioning principles of a "Wet" forest buffer zone for reducing concentrations and loads of glyphosate, isoproturon, metazachlor, azoxystrobin, epoxiconazole, and cyproconazole. A tracer injection experiment was conducted in the field in a forest buffer zone at Bray (France). A fine time-scale sampling enabled to illustrate that interactions between pesticides and forest buffer substrates (soil and organic-rich litter layer), had a retarding effect on molecule transfer. Low concentrations were observed for all pesticides at the forest buffer outlet thus demonstrating the efficiency of "Wet" forest buffer zone for pesticide dissipation. Pesticide masses injected in the forest buffer inlet directly determined concentration peaks observed at the outlet. Rapid and partially reversible adsorption was likely the major process affecting pesticide transfer for short retention times (a few hours to a few days). Remobilization of metazachlor, isoproturon, desmethylisoproturon, and AMPA was observed when non-contaminated water flows passed through the forest buffer. Our data suggest that pesticide sorption properties alone could not explain the complex reaction mechanisms that affected pesticide transfer in the forest buffer. Nevertheless, the thick layer of organic matter litter on the top of the forest soil was a key parameter, which enhanced partially reversible sorption of pesticide, thus retarded their transfer, decreased concentration peaks, and likely increased degradation of the pesticides. Consequently, to limit pesticide pollution transported by surface water, the use of already existing forest areas as buffer zones should be equally considered as the most commonly implemented grass buffer strips.

International audience | The remediation of copper-contaminated soils by aided phytostabilisation in 16 field plots at a wood preservation site was investigated. The mobility and bioavailability of four potentially toxic trace elements (PTTE), i.e., Cu, Zn, Cr, and As, were investigated in these soils 4 years after the incorporation of compost (OM, 5 % w/w) and dolomite limestone (DL, 0.2 % w/w), singly and in combination (OMDL), and the transplantation of mycorrhizal poplar and willows. Topsoil samples were collected in all field plots and potted in the laboratory. Total PTTE concentrations were determined in soil pore water (SPW) collected by Rhizon soil moisture samplers. Soil exposure intensity was assessed by Chelex100-DGT (diffusive gradient in thin films) probes. The PTTE phytoavailability was characterized by growing dwarf beans on potted soils and analyzing their foliar PTTE concentrations. OM and DL, singly and in combination (OMDL), were effective to decrease foliar Cu, Cr, Zn, and As concentrations of beans, the lowest values being numerically for the OM plants. The soil treatments did not reduce the Cu and Zn mineral masses of the bean primary leaves, but those of Cr and As decreased for the OM and DL plants. The Cu concentration in SPW was increased in the OM soil and remained unchanged in the DL and OMDL soils. The available Cu measured by DGT used to assess the soil exposure intensity correlated with the foliar Cu concentration. The Zn concentrations in SPW were reduced in the DL soil. All amendments increased As in the SPW. Based on DGT data, Cu availability was reduced in both OM and OMDL soils, while DL was the most effective to decrease soil Zn availability.

International audience | Phthalates are common atmospheric contaminantsused in the plastic industry. Ants have been shown to constitutegood bioindicators of phthalate pollution. Hence,phthalates remain trapped on ant cuticles which are mostlycoated with long-chain hydrocarbons. In this study, we artificiallycontaminated Lasius niger ants with four phthalates:dibutyl phthalate (DBP), diisobutyl phthalate (DiBP), di(2-ethylhexyl) phthalate (DEHP), and benzyl butyl phthalate(BBP). The first three have previously been found on ants innature in Touraine (France), while the fourth has not. The fourphthalates disappeared rapidly (less than 5 days) from thecuticles of live ants. In contrast, on the cuticles of dead ants,DEHP quantities remained unchanged over time. These resultsindicate that phthalates are actively absorbed by thecuticles of live ants. Cuticular absorption of phthalates isnonspecific because eicosane, a nonnatural hydrocarbon onL. niger cuticle, was similarly absorbed. Ants are importantecological engineers and may serve as bioindicators of ecosystemhealth. We also suggest that ants and more generallyterrestrial arthropods may contribute to the removal ofphthalates from the local environment

The insecticide chlordecone is a contaminant found in most of the banana plantations in the French West Indies. This study aims to search for fungal populations able to grow on it. An Andosol heavily contaminated with chlordecone, perfused for 1 year in a soil–charcoal system, was used to conduct enrichment cultures. A total of 103 fungal strains able to grow on chlordecone-mineral salt medium were isolated, purified, and deposited in the MIAE collection (Microorganismes d'Intérêt Agro-Environnemental, UMR Agroécologie, Institut National de la Recherche Agronomique, Dijon, France). Internal transcribed spacer sequencing revealed that all isolated strains belonged to the Ascomycota phylum and gathered in 11 genera: Metacordyceps, Cordyceps, Pochonia, Acremonium, Fusarium, Paecilomyces, Ophiocordyceps, Purpureocillium, Bionectria, Penicillium, and Aspergillus. Among predominant species, only one isolate, Fusarium oxysporum MIAE01197, was able to grow in a liquid culture medium that contained chlordecone as sole carbon source. Chlordecone increased F. oxysporum MIAE01197 growth rate, attesting for its tolerance to this organochlorine. Moreover, F. oxysporum MIAE01197 exhibited a higher EC50 value than the reference strain F. oxysporum MIAE00047. This further suggests its adaptation to chlordecone tolerance up to 29.2 mg l−1. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that 40 % of chlordecone was dissipated in F. oxysporum MIAE01197 suspension culture. No chlordecone metabolite was detected by GC-MS. However, weak amount of 14CO2 evolved from 14C10-chlordecone and 14C10-metabolites were observed. Sorption of 14C10-chlordecone onto fungal biomass followed a linear relationship (r 2 = 0.99) suggesting that it may also account for chlordecone dissipation in F. oxysporum MIAE01197 culture