The present study aimed to show that nickel and fluoride exhibited synchronized co-inhibited uptake in the aromatic rice cultivar, Gobindobhog, since bioaccumulation of the two elements was lower than that during individual stress, so that overall growth under combined stress was similar to control seedlings. On the contrary, lead and fluoride stimulated their co-uptake which triggered oxidative damages, NADPH oxidase activity, methylglyoxal accumulation, photosynthetic inhibition, membrane-protein damages, necrosis and genomic template degradation. Accumulation of proline, anthocyanins, non-protein thiols and phytochelatins was stimulated for systemic protection against reactive oxygen species (ROS) and xenobiotic-mediated injuries during lead-fluoride toxicity. ROS accumulation during nickel-fluoride stress was insignificant due to which enhanced accumulation of most antioxidants was not required. Glutathione depletion during combined lead-fluoride toxicity was due to its utilization in the glyoxalase cycle and also inhibition of glutathione reductase. However, the nickel-fluoride-treated sets maintained glutathione reserves and glyoxalase activity similar to those in control. Presence of fluoride ‘safeguarded’ the glutathione-utilizing enzymes like glutathione reductase, glutathione peroxidase and glutathione-S-transferase during dual lead-fluoride stress. This was because these enzymes showed higher activity compared to that under lead toxicity alone. Enzymatic antioxidants like superoxide dismutase, ascorbate peroxidase and guaiacol peroxidase were activated during lead-fluoride toxicity due to altered iron and copper homeostasis. Catalase activity was strongly inhibited, resulting in the inability to scavenge H₂O₂ and suppression of the fluoride-adaptable phenotype. However, none of the enzymatic antioxidants were inhibited during nickel-fluoride stress, which cumulatively allowed the seedlings to maintain normal physiology. Overall our findings holistically reveal the physiological plasticity of Gobindobhog in response to two different heavy metals under the influence of fluoride.

Conservation of soil health and crop productivity is the central theme for sustainable agriculture practices. It is unrealistic to expect that the burgeoning crop production demands will be met by a soil ecosystem that is increasingly unhealthy and constrained. Therefore, the present review is focused on soil amendment techniques, using biochar in combination with arbuscular mycorrhizal fungi (AMF), which is an indispensable biotic component that maintains plant-soil continuum. Globally significant progress has been made in elucidating the physical and chemical properties of biochar; along with its role in carbon sequestration. Similarly, research advances on AMF include its evolutionary background, functions, and vital roles in the soil ecosystem. The present review deliberates on the premise that biochar and AMF have the potential to become cardinal to management of agro-ecosystems. The wider perspectives of various agronomical and environmental backgrounds are discussed. The present state of knowledge, different aspects and limitations of combined biochar and AMF applications (BC + AMF), mechanisms of interaction between biochar and AMF, effects on plant growth, challenges and future opportunities of BC + AMF applications are critically reviewed. Given the severely constrained nature of soil health, the roles of BC + AMF in agriculture, bioremediation and ecology have also been examined. In spite of the potential benefits, the functionality and dynamics of BC + AMF in soil are far from being fully elucidated.

Xenobiotics are worldwide distributed and humans are unavoidably exposed to multiple chemical compounds during life, from preconception to adulthood. The human microbiota is mainly settled during early life and modulate host health and fitness. One of the main routes for chemical exposure is by intake of contaminated food and water. Thus, the interplay between diet-xenobiotics-microbiota during pregnancy and perinatal period may have relevant consequences for infant and adult health. Maternal exposure to metal(oid)s, persistent organic pollutants, and some food additives can modify the infant’s microbiota with unknown consequences for child or adult health. Toxicants’ exposure may also modulate the maternal transfer of microorganisms to the progeny during birth and breastfeeding; however, scarce information is available. The rapid increase in releasing novel chemicals to the environment, the exposure to chemical mixtures, the chronic/low dose scenario, and the delay in science-stakeholders action call for novel and groundbreaking approaches to improve a comprehensive risk assessment in sensitive population groups like pregnant women and neonates, with emphasis on microbiota as modulating factor and target-organ of xenobiotic’s toxicity.

Microplastics are continuously released into the terrestrial environment from sources where they are used and produced. These microplastics accumulate in soils, sediments, and freshwater bodies, and some are conveyed via wind and water to the oceans. The concentration gradient between terrestrial inland and coastal regions, the factors that influence the concentration, and the fundamental transport processes that could dynamically affect the distribution of microplastics are unclear. We analyzed microplastic concentration reported in 196 studies from 49 countries or territories from all continents and found that microplastic concentrations in soils or sediments and surface water could vary by up to eight orders of magnitude. Mean microplastic concentrations in inland locations such as glacier (191 n L⁻¹) and urban stormwater (55 n L⁻¹) were up to two orders of magnitude greater than the concentrations in rivers (0.63 n L⁻¹) that convey microplastics from inland locations to water bodies in terrestrial boundary such as estuaries (0.15 n L⁻¹). However, only 20% of studies reported microplastics below 20 μm, indicating the concentration in these systems can change with the improvement of microplastic detection technology. Analysis of data from laboratory studies reveals that biodegradation can also reduce the concentration and size of deposited microplastics in the terrestrial environment. Fiber percentage was higher in the sediments in the coastal areas than the sediments in inland water bodies, indicating fibers are preferentially transported to the terrestrial boundary. Finally, we provide theoretical frameworks to predict microplastics transport and identify potential hotspots where microplastics may accumulate.

Current assessments of human exposure to flame retardants (FRs) via dust ingestion rely on measurements of FR concentrations in dust samples collected at specific points in time and space. Such exposure assessments are rendered further uncertain by the possibility of within-room and within-building spatial and temporal variability, differences in dust particle size fraction analysed, as well as differences in dust sampling approach. A meta-analysis of peer-reviewed data was undertaken to evaluate the impact of these factors on reported concentrations of brominated flame retardants (BFRs) and organophosphate esters (OPEs) in dust and subsequent human exposure estimates. Except for a few cases, concentrations of FRs in elevated surface dust (ESD) exceeded significantly those in floor dust (FD). The implications of this for exposure assessment are not entirely clear. However, they imply that analysing FD only will underestimate exposure for adults who likely rarely ingest floor dust, while analysing ESD only would overestimate exposure for toddlers who likely rarely ingest elevated surface dust. Considerable within-building spatial variability was observed with no specific trend between concentrations of either BFRs or OPEs in living rooms and bedrooms in the same homes, implying that exposure assessments based solely on sampling one room are uncertain. Substantial differences in FR concentrations were observed in different particle size fractions of dust. This is likely partly attributable to the presence of abraded polymer particles/fibres with high FR concentrations in larger particle size fractions. This has implications for exposure assessment as adherence to skin and subsequent FR uptake via ingestion and dermal sorption varies with particle size. Analysing dust samples obtained from a householder vacuum cleaner (HHVC) compared with researcher collected dust (RCD) will underestimate human exposure to the most of studied contaminants. This is likely due to the losses of volatile FRs from HHVC dust over the extended period such dust spends in the dust bag. Temporal variability in FR concentrations is apparent during month-to-month or seasonal monitoring, with such variability likely due more to changes in room contents rather than seasonal temperature variation.

Plant uptake is an important process in determining the transfer of pesticides through a food chain. Understanding how crops take up and translocate pesticides is critical in developing powerful models to predict pesticide accumulation in agricultural produce and potential human exposure. Herein, wheat was selected as a model plant species to investigate the uptake and distribution of eleven widely used pesticides in a hydroponic system as a function of time for 144 h. The time-dependent uptake kinetics of these pesticides were fitted with a first-order 1-compartment kinetic model. During 144 h, flusilazole and difenoconazole, with relative high log Kₒw (3.87 and 4.36, respectively), displayed higher root uptake rate constants (k). To clarify the role of root lipid content (fₗᵢₚ) in plant accumulation of pesticides, we conducted a lipid normalization meta-analysis using data from this and previous studies, and found that the fₗᵢₚ value was an important factor in predicting the root concentration factor (RCF) of pesticides. An improved correlation was observed between log RCF and log fₗᵢₚKₒw (R² = 0.748, N = 26, P < 0.001), compared with the correlation between log RCF and log Kₒw (R² = 0.686, N = 26, P < 0.001). Furthermore, the hydrophilic pesticides (e.g. log Kₒw < 2) were found to reach partition equilibrium faster than lipophilic pesticides (e.g. log Kₒw > 3) during the uptake process. The quasi-equilibrium factor (αₚₜ) was inversely related to log Kₒw (R² = 0.773, N = 11, P < 0.001) suggesting a hydrophobicity-regulated uptake equilibrium. Findings from this study could facilitate crop-uptake model optimization.

Arsenic (As) is a toxic pollutant that mainly enters the human body via plants. Therefore, understanding the strategy for reducing arsenic accumulation in plants is important to human health and the environment. Aquaporins are ubiquitous water channel proteins that bidirectionally transport water across cell membranes and play a role in the transportation of other molecules, such as glycerol, ammonia, boric acid, and arsenic acid. Previously, we observed that Arabidopsis PIP2;2, encoding a plasma membrane intrinsic protein, is highly expressed in NtCyc07-expressing Arabidopsis, which shows a higher tolerance to arsenite (As(III)). In this study, we report that the overexpression of AtPIP2;2 enhanced As(III) tolerance and reduced As(III) levels in yeast. Likewise, AtPIP2;2-overexpressing Arabidopsis exhibited improved As(III) tolerance and lower accumulation of As(III). In contrast, atpip2;2 knockout Arabidopsis showed reduced As(III) tolerance but no significant change in As(III) levels. Interestingly, the AtPIP2;2 transcript and protein levels were increased in roots and shoots of Arabidopsis in response to As(III). Furthermore, As(III) efflux was enhanced and As(III) influx/accumulation was reduced in AtPIP2;2-expressing plants. The expression of AtPIP2;2 rescued the As(III)-sensitive phenotype of acr3 mutant yeast by reducing As levels and slightly reduced the As(III)-tolerant phenotype of fps1 mutant yeast by enhancing As content, suggesting that AtPIP2; 2 functions as a bidirectional channel of As(III), while the As(III) exporter activity is higher than the As(III) importer activity. All these results indicate that AtPIP2;2 expression promotes As(III) tolerance by decreasing As(III) accumulation through enhancing As(III) efflux in Arabidopsis. This finding can be applied to the generation of low arsenic crops for human health.

Pesticides may alter soil microbial community structure or diversity, but their impact on microbial co-occurrence patterns remains unclear. Here, the effect of the widely used neonicotinoid insecticide thiamethoxam on the bacterial community in five arable soils was deciphered using the 16S rRNA gene amplicon sequencing technique. The degradation half-life of thiamethoxam in nonsterilized soils was significantly lower than that in sterilized soils, suggesting a considerable contribution from biodegradation. Soil bacterial community diversity diminished in high concentration thiamethoxam treatment and its impact varied with treatment concentration and soil type. Bacterial co-occurrence network complexity significantly decreased after exposure to thiamethoxam. Under thiamethoxam stress, the relative changes in bacterial co-occurrence networks were closely related (the majority of p-values < 0.05) to the soil physicochemical properties, yet the diversity and dominant phyla were slightly related (the majority of p-values > 0.05). Additionally, three bacterial genera, Sphingomonas, Streptomyces, and Catenulispora, were identified to be relevant to the degradation of thiamethoxam in soils. This finding deciphers the succession of the bacterial community under thiamethoxam stress across multiple soils, and emphasizes the potential role of physicochemical properties in regulating the ecotoxicological effect of pesticides on the soil microbiome.

The removal of halogenated dye and sensing of pharmaceutical products in the water bodies with quick purification time is of high need due to the scarcity of drinking water. The present work reported on the preparation of graphitic carbon nitride (g-C₃N₄) for quick time water contaminant adsorption, followed by synthesizing silver nanoparticles decorated graphitic carbon nitride for pharmaceutical product sensing using in-situ SERS technique. The prepared graphitic carbon nitride is used to study the adsorption behavior of water contaminants at room temperature, in the presence of methylene blue (MB) as an adsorbate model. The water-soluble graphitic carbon nitride, even at low concentration, possesses an excellent ability to adsorb halogenated organic dye. As a result, the dyes are found to adsorb within ∼5s even without any additional physical or chemical activation. From the UV–Vis absorption investigations, it has been perceived that in the presence of graphitic carbon nitride (g-C₃N₄) the dye adsorption efficacy is observed nearly 80% with the well fitted linearly of R² = 0.9731. Effective in-situ surface-enhanced Raman scattering (SERS) studies for Ag nanoparticles decorated graphitic carbon nitride has been carried out and the obtained result shows good sensing performance of the material towards acetaminophen drug. This method opens the possibility of the Nobel metal decorated graphitic carbon nitride for real-time sensing of SERS-based drug products along with the development of high-performance sensing of the target analyte in the future.

Bisphenol A (BPA) is a contaminant whose presence in aquatic environments is increasing. In fish embryos and larvae, it severely affects cardiac development; however, its influence on the heart function of adult fish has been scarcely analyzed. This study investigated the effects of the in vivo exposure to BPA on heart physiology, morphology, and oxidative balance in the goldfish Carassius auratus. Adult fish were exposed for 4 and 10 days to two BPA concentrations (10 μM and 25 μM). Ex vivo working heart preparations showed that high concentrations of BPA negatively affected cardiac hemodynamics, as revealed by an impaired Frank-Starling response. This was paralleled by increased cardio-somatic indices and by myocardial structural changes. An altered oxidative status and a modulation of stress (HSPs) and pro-apoptotic (Bax and Cytochrome C) proteins expression were also observed in the heart of animals exposed to BPA, with detrimental effects at the highest concentration and the longest exposure time. Results suggest that, in the adult goldfish, BPA may induce stressful conditions to the heart with time- and concentration-dependent deleterious morpho-functional alterations.