A set of laboratory experiments was conducted to assess the trapping efficiency of buoyant plastic debris by the estuarine vegetation Spartina maritima. Different hydrodynamic conditions typical of salt marshes were simulated in a hydraulic flume. These conditions included varying water levels between 40% and 90% of stem height, wind speeds up to 2 m/s, and unidirectional current velocities between 0.1 and 0.3 m/s. Moreover, three vegetation densities (small/medium/high) and nine plastic debris types varying in shape (elongated/two-dimensional/three-dimensional) and size (macro/meso) were tested. The results indicate that Spartina maritima functions as a natural trap. Specifically, the study highlights that lower surface velocities, higher stem densities, greater emergent heights, and larger debris sizes significantly enhance trapping efficiency. It was also inferred that for a Spartina maritima density comparable to that observed in marshes, the dominance of surface velocity or debris size effects on trapping efficiency is primarily dependent on debris shape. Consequently, surface velocity has a greater impact on two-dimensional elements, whereas debris size is more significant for three-dimensional elements. Finally, a preliminary trapping model was developed to integrate all the aforementioned variables. This model has the potential to enhance the accuracy of numerical predictions regarding the transport and fate of plastic debris using Lagrangian modeling, and can be further refined by incorporating additional data. | The authors acknowledge the support of the Programa de Ciencias Marinas (PCM), funded by the Ministry of Science and Innovation of the Spanish Government and the Regional Governments of Andalucía, Baleares, Canarias, Cantabria, Comunidad Valenciana, Galicia, and Murcia (PRTR-C17⋅I1). This program, part of the ThinkInAzul I + D + i Complementary Plan, has provided essential funding for this research, in line with the 2030 Agenda for Sustainable Development and the Decade of the Oceans initiative. Moreover, this research was also partially funded by PID2021-127358NB-I00-MICIU/AEI/10.13039/501100011033 and by FEDER as a way of making Europe. Finally, we express our deep gratitude to Margot Sánchez for her invaluable support and dedication throughout the development of this work.

peer reviewed | This study investigates essential (Mg, Ca, Fe, Mn, Cu, Zn, Se, Ni) and non-essential (Li, Be, Cr, Rb, Sr, Cs, Cd, Sn, Ba, and Pb) element concentrations and stable isotope (δ13C, δ15N, δ34S) compositions in feathers of Brown Boobies (Sula leucogaster) from three distinct Atlantic islands: the Archipelagos of Saint Peter and Saint Paul (SPSP), Abrolhos, and Cagarras. We aimed to investigate the ecological and environmental factors influencing these seabird populations and assess potential variations in contaminant exposure and dietary habits based on location, sex, and maturity stages. Our finding revealed significant geographical differences in trace element concentrations. The Brown Boobies from Cagarras had higher concentrations (mean ± SD, μg g-1) of Fe (29 ± 20) and Mn (0.82 ± 0.82) than those from Abrolhos (Fe: 21 ± 20; Mn: 0.24 ± 0.09) and SPSP (Fe: 15 ± 16; Mn: 0.21 ± 0.06). Tin concentrations were also higher in Cagarras (Sn: 0.02 ± 0.01) than in SPSP (Sn: 0.01 ± 0.01). Our analyses revealed significant differences in Li, Mg, Rb, and Zn concentrations between adults and juveniles. However, there were no sex-related differences in element concentrations within each locality. SIBER analyses revealed distinct dietary differences among the three Brown Boobies populations, with the Cagarras seabirds occupying a higher trophic position compared to the SPSP population. This study highlights the importance of considering different populations to understand contaminant exposure and ecological dynamics in Brown Boobies along the South Atlantic. The Cagarras population shows significantly higher contaminant levels, likely due to proximity to anthropogenic activities. These results highlight the necessity for ongoing monitoring to evaluate long-term effects on the more impacted population and to ensure seabird health and sustainability in the Atlantic Ocean.

Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds. | Instituto de Recursos Biológicos | Fil: Fernández-Vizcaíno, Elena. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España | Fil: Poliserpi, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina | Fil: Ortiz-Santaliestra, Manuel E. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España | Fil: Mougeot, Francois. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España | Fil: Mateo, Rafael. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España. CSIC. Institute for Environmental Assessment and Water Research (IDAEA); España | Fil: Camarero, Pablo R. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España | Fil: Brodeur, Julie C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina | Fil: Brodeur, Julie C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

peer reviewed | Accurate interpretation of mercury (Hg) isotopic data requires the consideration of several biotic factors such as age, diet, geographical range, and tissue metabolic turnover. A priori knowledge of prey-predator isotopic incorporation rates and Hg biomagnification is essential. This study aims to assess Hg stable isotopes incorporation in an Arctic species of Phocidae, the hooded seal Cystophora cristata, kept in human care for 24 months (2012 - 2014) and fed on a constant diet of Norwegian Spring Spawning herring Clupea harengus. We measured THg, MMHg and iHg levels, as well as Hg stable isotope composition with both mass dependent (MDF) and mass independent (MIF) fractionation (e.g. δ202Hg and Δ199,200,201,204Hg) in hooded seal kidney, liver, hair and muscle, in addition to herring muscle. We then calculated Hg MDF and MIF isotopic fractionation between hooded seals and their prey. We found a significant shift in δ202Hg between hooded seal hair (+0.80‰) and kidney (-0.78‰), and herring muscle. In hooded seals tissues δ202Hg correlated positively with MMHg percentage. These findings suggest that tissue-specific Hg speciation is the major driver of changes in Hg isotopic fractionation rates in this Arctic predator. Δ199Hg, Δ200Hg, Δ201Hg and Δ204Hg values did not vary between herring and hooded seal tissues, confirming their utility as tracers of Hg marine and atmospheric sources in top predators. To our knowledge, this represents the first attempt to assess complex Hg isotope dynamics in the internal system of Arctic Phocidae, controlling the effects of age, diet, and distribution. Our results confirm the validity of Hg stable isotopes as tracers of environmental Hg sources even in top predators, but emphasize the importance of animal age and tissue selection for inter-study and inter-species comparisons. | 14. Life below water

peer reviewed | Metconazole (MEZ), a chiral triazole fungicide, produces enantioselective adverse effects in non-target organisms. Among MEZ's isomers, cis-MEZ displays robust antimicrobial properties. Evaluating MEZ and cis-MEZ's toxicity may mitigate fungicide usage and safeguard non-target organisms. Our study evaluated the toxicity of MEZ and its cis-isomers at concentrations of 0.02, 0.2, 2, and 4 mg L−1. We report stereoselectivity and severe cardiovascular defects in zebrafish, including pericardial oedema, decreased heart rate, increased sinus venous and bulbous arteries distances, intersegmental vessel defects, and altered cardiovascular development genes (hand2, gata4, nkx2.5, tbx5, vmhc, amhc, dll4, vegfaa, and vegfc). Further, MEZ significantly increased oxidative stress and apoptosis in zebrafish, primarily in the cardiac region. Isoquercetin, an antioxidant found in plants, partially mitigates MEZ-induced cardiac defects. Furthermore, MEZ upregulated the Wnt/β-catenin pathway genes (wnt3, β-catenin, axin2, and gsk-3β) and β-catenin protein expression. Inhibitor of Wnt Response-1 (IWR-1) rescued MEZ-induced cardiotoxicity. Our findings highlight oxidative stress, altered cardiovascular development genes, and upregulated Wnt/β-catenin signaling as contributors to cardiovascular toxicity in response to MEZ and cis-MEZ treatments. Importantly, 1R,5S-MEZ exhibited greater cardiotoxicity than 1S,5R-MEZ. Thus, our study provides a comprehensive understanding of cis-MEZ's cardiovascular toxicity in aquatic life. © 2024 Elsevier Ltd

Temporal trends for concentrations of mercury (Hg), lead (Pb) and cadmium (Cd) were evaluated from year 2000–2020 in 20 (Hg), 23 (Pb) and 11 (Cd) watercourses in remote forest catchments in Europe. Decreasing trends were observed in 15% (Hg), 39% (Pb) and 45% (Cd) of the watercourses during the period of evaluation. Decreasing trends were mainly observed between 2000 and 2005 for Hg and between 2000 and 2015 for Pb and Cd. For the last five years of the studied time period (2015–2020), more watercourses showed significant increasing, rather than decreasing Hg, Pb and Cd trends. This was interpreted as a legacy effect of metals still retained in catchment soils. The overall negative trends during the earlier part of the study period were likely driven by declining deposition of metals over Europe, especially for Pb and Cd. Other changes related to metal transport and chemistry may have contributed to the observed trends as well, including recovery from acidification and the ongoing browning of surface waters at northern latitudes. Here we found that organic carbon could explain the seasonal variation in Hg and Pb, but was not related the interannual trends. This study highlights the need for long-term monitoring and robust statistical methods that can detect multidirectional, long-term change in water chemistry. | publishedVersion

In December 2023, 25 tons of pellets were lost by the Toconao ship in the Northwest Atlantic Ocean in front of the Portuguese coast. In this work, a coastal stretch of 633 km in Asturias and Galicia (Spain) and Northern Portugal was investigated to assess pellets’ concentration on 31 beaches. Field surveys were carried out in March 2024 and focused on sampling plastic pellets deposited along the shoreline. All the 7263 sampled pellets were characterized by size, degradation level, and color, while one subset was characterized by weight (40 % of the total) and another subset by polymer type (15 % of the total) using FT-IR spectroscopy. The results reveal that 94 % of the surveyed sites containing pellets, whereas the concentration values vary significantly among beaches, ranging from 0 to 40.3 pellets/kgdw. By combining the accounted variables, it emerges that 48.0 % of the collected pellets can be linked to the Toconao spill.

After marine oil spills, natural processes like photooxidation and biodegradation can remove the oil from the environment. However, these processes are strongly influenced by environmental conditions. To achieve a greater understanding of how seasonal variations in temperature, light exposure and the bacterial community affect oil depletion in the marine environment, we performed two field experiments during the spring and autumn. Field systems equipped with a thin oil film of Statfjord, Grane or ULSFO were deployed in northern Norway. Depletion of the total extractable matter was faster during the spring than during the autumn. Statfjord showed faster depletion of n-alkanes during spring, while depletion of polycyclic aromatic hydrocarbons varied between the seasons based on the degree of alkyl-substitutions. ULSFO displayed the overall slowest depletion. Biodegradation of the oils was associated with high abundances of unassigned bacteria during the spring but was governed by Alcanivorax, Cycloclasticus, Oleibacter and Oleispira during the autumn. | publishedVersion

Plastic debris is a significant threat to marine and coastal ecosystems. Previous research found that waves, wind, as well as density, size, and shape of microplastics, drive their transport and dispersion. In this paper, a set of laboratory experiments on the effect of waves and wave-induced currents on the input rate and cross-shore transport and dispersion of different types of plastic debris, including the macro and mesosizes, in addition to microplastics is presented. 15 plastic-debris types characterized by different sizes, shapes, and densities, including facemasks, were analyzed under regular and irregular wave conditions. The results show that input and transport rates of plastics depend on their terminal velocities and wave steepness. Plastics with higher settling velocities under less-steep wave conditions are likely to escape coastal entrapment and end up in the breaking zone. However, plastics with greater buoyancy rates under steeper waves show a predominant accumulation closer to the shoreline. | P.N. is supported by a Margarita Salas post-doctoral fellowship funded by 638 European Union-NextGenerationEU,Ministry of Universities and Recovery Trans639 formation and Resilience Plan, through a call fromthe University of Cantabria. The 640 financial support from the Government of Cantabria through the FÉNIX Program 641 (ID 2020.03.03.322B.742.09) is warmly acknowledged. We acknowledge M.S. and the IHLab-Hydro team for all the technical support.