Microplastics, an emerging pollutant, are recognised as having a ubiquitous distribution in the environment. Currently several benthic sampling tools are being employed to collect subtidal marine sediment, however, there are no comparative studies on the efficiency of these tools to sample for microplastics or the subsequent extraction methods of microplastics from these marine sediments. This study addresses these knowledge gaps by comparing commonly applied benthic sampling tools (Van Veen grab, box corer, gravity corer) and a variety of density separation methods (elutriation column, sodium chloride solution, sodium tungstate dihydrate solution) for microplastic collection and processing.Each sampling tool was tested at the same station and the collected sediment was used to assess the extraction performance for the different density separation techniques. No statistically significant differences were found between the concentrations of microplastics extracted for any of the sampling tools. However, there were significant differences between the density separation methods using sodium tungstate dihydrate and sodium chloride solution and the elutriation method. This preliminary study provides evidence that the sampling tools tested are both suitable and proficient at determining the abundance of microplastics in sediments. Sodium tungstate dihydrate proved to be a novel and feasible option for dense liquid separation of microplastics in subtidal marine sediments. These results will allow for more confidence in data quality when comparing future surveys applying different benthic sampling tools.

Water quality in Lake Pontchartrain was deteriorating and recreational activities along the beach were restricted by the end of the 20th Century. A microbial source tracking (MST) study was conducted to determine the fecal contamination sources at public beach of the lake, so that effective pollution control strategies can be developed. Water samples were collected over an eight-month period at ten locations along the lake in 2016 and 2017. E. coli and Enterococcus were detected in 90.6% (culture) and 97.5% (qPCR), 95.8% (culture) and 91.8% (qPCR) of water samples from all sampling sites, respectively. Significant positive relationship between E. coli and Enterococcus results was observed for both qPCR and culture methods. HF183 marker was detected in 94.3% water samples (149 of 158), with concentrations ranging from 29.0 to 6073.5GC/100ml and from 129.8 to 38,465.6GC/100ml in summer and winter, respectively. The results also indicate that significant rainfall events have the potential to supply considerable loads of fecal bacteria to lake waters. Further research is needed to determine the contribution of other animals to fecal contamination in the region.

Mercury (Hg) is a non-essential, toxic metal that is distributed worldwide. Mercury biomagnifies in food webs and can threaten the health of top predators such as seabirds. The Kittlitz's murrelet (Brachyramphus brevirostris) is a seabird endemic to Alaska and the Russian Far East and is a species of conservation concern in the region. We determined Hg concentrations in eggshells, guano, blood, and feathers of Kittlitz's murrelets sampled from four locations in Alaska. Mercury concentrations in eggshells, guano, and blood were low compared to other seabird species. Mean Hg concentrations of breast feathers from Adak Island and Glacier Bay were significantly greater than those from Agattu Island or Icy Bay. Two Kittlitz's murrelets at Glacier Bay and one Kittlitz's murrelet at Adak Island had Hg concentrations above those associated with impaired reproduction in other bird species, and may merit further investigation as a potential threat to individuals and populations.

The purpose of the present study was to analyze the concentrations of lead, cadmium and mercury in mussels (Mytilus galloprovincialis) and veined rapa whelks (Rapana venosa) caught in the Varna Bay of Black Sea and to evaluate the risk for human health from the presence of the three heavy metals. The highest average concentrations in mussels were those of cadmium (0.280 mg/kg), followed by lead (0.251 mg/kg) and mercury (0.017 mg/kg). Veined rapa whelks also showed highest levels of cadmium (1.113 mg/kg), followed by lead (0.045 mg/kg) and mercury (0.034 mg/kg). EDI values for adults consuming mussels and veined rapa whelks were below the published RfDo and PTWI values. All THQ and HI values were below 1. The consumption of M. galloprovincialis and R. venosa caught in the Varna Bay, Black Sea, did not pose any risk for the health of adult people as lead, cadmium and mercury were concerned.

Coastal ecosystem structures and functions are changing under natural and anthropogenic influences. In this study, surface sediment samples were collected from disturbed zone (DZ), near estuary zone (NEZ), and far estuary zone (FEZ) of Hangzhou Bay, one of the most seriously polluted bays in China. The bacterial community structures and predicted functions varied significantly in different zones. Firmicutes were found most abundantly in DZ, highlighting the impacts of anthropogenic activities. Sediment total phosphorus was most influential on the bacterial community structures. Predicted by PICRUSt analysis, DZ significantly exceeded FEZ and NEZ in the subcategory of Xenobiotics Biodegradation and Metabolism; and DZ enriched all the nitrate reduction related genes, except nrfA gene. Seawater salinity and inorganic nitrogen, respectively as the representative natural and anthropogenic factor, performed exact-oppositely in nitrogen metabolism functions. The changes of bacterial community compositions and predicted functions provide a new insight into human-induced pollution impacts on coastal ecosystem.

Commercial marine fish, crustacean, and mollusc samples were collected from Sanmen Bay and its adjacent areas to investigate the accumulation of heavy metals in various species of marine organisms and evaluate the potential health risk for local consumers. The results indicated significant variations in metal contents among species. The highest concentrations of studied metals were found in molluscs, followed by crustaceans and fish. The first metal group of arsenic (As), cadmium, copper, and zinc were associated by their relatively high concentrations in the tissues of marine organisms, whereas chromium, mercury, and lead were identified the other group with low concentrations. Human health risk evaluation indicated that the exposure doses of most elements for coastal people were safe, except for As, which scored a high total target hazard quotient and target cancer risk value. Potential health risk of heavy metal exposure from seafood consumption should not be ignored.

Severe microplastic pollution from anthropogenic activities in coastal zones presents an imminent risk to marine ecosystems. In this study, abundant microplastics (15–12,852 items kg−1) with sizes ranging between 0.16 and 5.0 mm were extracted from 17 sediment samples collected in sandy beaches and mangrove wetlands of the Qinzhou Bay, Guangxi Province, Southwest China. Three types of microplastics (i.e. polystyrene, polypropylene, and polyethylene) were identified with Fourier transform infrared (FTIR) spectroscopy analysis. These detected microplastics were characterized by different colors (white, transparent, yellow, green, red, and blue) and shapes (fragment, fiber, and sphere). Microplastics were concentrated on supratidal beaches and wetlands outside of mangrove, and less abundant on intertidal beaches and inside of mangrove wetlands. Meanwhile, high microplastic concentrations were observed near mollusk farms. The spatial distribution and chemical speciation indicated that microplastics were derived from disintegration of large plastic debris (e.g., Styrofoam buoys used to support mollusk rafts) abandoned by aquaculture industry. Further, coastal vegetation (e.g. mangrove) could trap microplastic particles.

Standardized ecosystem-based monitoring surveys are critical for providing information on marine ecosystem health. Environmental DNA/RNA (eDNA/eRNA) metabarcoding may facilitate such surveys by quickly and effectively characterizing multi-trophic levels. In this study, we assessed the suitability of eDNA/eRNA metabarcoding to evaluate changes in benthic assemblages of bacteria, Foraminifera and other eukaryotes along transects at three offshore oil and gas (O&G) drilling and production sites, and compared these to morphologically characterized macro-faunal assemblages. Bacterial communities were the most responsive to O&G activities, followed by Foraminifera, and macro-fauna (the latter assessed by morphology). The molecular approach enabled detection of hydrocarbon degrading taxa such as the bacteria Alcanivorax and Microbulbifer at petroleum impacted stations. Most identified indicator taxa, notably among macro-fauna, were highly specific to site conditions. Based on our results we suggest that eDNA/eRNA metabarcoding can be used as a stand-alone method for biodiversity assessment or as a complement to morphology-based monitoring approaches.