The aims of the present study were to investigate Pb and Cd levels in tissues of wild boar (Sus scrofa) from the Sierra Nevada Natural Space (SNNS) (southern Spain). Heavy metal concentrations in livers, kidneys and bones from 111 animals were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Bones and kidneys were the most Pb- and Cd-contaminated tissues, respectively; Cd concentrations were 5.6 times higher in kidneys than in livers. This is the first biomonitoring study of these pollutants in wild boar tissues in the SNNS, and findings indicate that this population is chronically exposed to these heavy metals. The detected Pb and Cd concentrations were lower than those found in many studies performed in Europe on the same species.

Sulfonamide antibiotics (SAs) have been observed to undergo direct and indirect photodegradation in natural water environments. In this study, the density functional theory (DFT) method was employed for the study of direct and indirect photodegradation mechanisms of sulfameter (SME) with excited triplet states of dissolved organic matter (³DOM*) and metal ions. SME was adopted as a representative of SAs, and SO₂ extrusion product was obtained with different energy paths in the triplet-sensitized photodegradation of the neutral (SME⁰) and the anionic (SME⁻) form of SME. The selected divalent metal ions (Ca²⁺, Mg²⁺, and Zn²⁺) promoted the triplet-sensitized photodegradation of SME⁰ but showed an inhibitory effect in triplet-sensitized photodegradation of SME⁻. The triplet-sensitized indirect photodegradation mechanism of SME was investigated with the three DOM analogues, i.e., 2-acetonaphthone (2-AN), fluorenone (FN), and thioxanthone (TN). Results indicated that the selected DOM analogues are highly responsible for the photodegradation via attacking on amine moiety of SME. According to the natural bond orbital (NBO) analysis, the triplet-sensitized photodegradation mechanism of SME⁰ with 2-AN, FN, and TN was H-transfer, and the SME⁻ was proton plus electron transfer with these DOM analogues.

The efficacies of catalyzed H₂O₂, activated persulfate, and catalyzed H₂O₂-persulfate processes for the degradation of ortho-nitrochlorobenzene (o-NCB) in soil were investigated. The application of catalyzed H₂O₂-persulfate process was promising, and after a careful adjustment of oxidants and activator doses, it demonstrated a considerable improvement in o-NCB degradation compared with activated persulfate process and catalyzed H₂O₂ process. The degradation of o-NCB in catalyzed H₂O₂-persulfate process was obviously influenced by the concentration of persulfate and H₂O₂, the molar ratio between persulfate and H₂O₂, the concentration of o-NCB, and initial pH. Degradation of o-NCB was obviously inhibited by the addition of tert-butyl alcohol, methanol, and phenol, suggesting that nitrobenzene was dominantly oxidized by HO· and SO₄ ⁻· generated in the catalyzed H₂O₂-persulfate process. The results from these studies demonstrated that the natural iron species present in soil could effectively facilitate the degradation of organic pollutants in the presence of H₂O₂ and persulfate.

In the shallow environment, the nutrient and carbon exchanges at the sediment–water interface contribute significantly to determine the trophic status of the whole water column. The intensity of the allochthonous input in a coastal environment subjected to strong anthropogenic pressures determines an increase in the benthic oxygen demand leading to depressed oxygen levels in the bottom waters. Anoxic conditions resulting from organic enrichment can enhance the exchange of nutrients between sediments and the overlying water. In the present study, carbon and nutrient fluxes at the sediment–water interface were measured at two experimental sites, one highly and one moderately contaminated, as reference point. In situ benthic flux measurements of dissolved species (O₂, DIC, DOC, N-NO₃ ⁻, N-NO₂ ⁻, N-NH₄ ⁺, P-PO₄ ³⁻, Si-Si(OH)₄, H₂S) were conducted using benthic chambers. Furthermore, undisturbed sediment cores were collected for analyses of total and organic C, total N, and biopolymeric carbon (carbohydrates, proteins, and lipids) as well as of dissolved species in porewaters and supernatant in order to calculate the diffusive fluxes. The sediments were characterized by suboxic to anoxic conditions with redox values more negative in the highly contaminated site, which was also characterized by higher biopolymeric carbon content (most of all lipids), lower C/N ratios and generally higher diffusive fluxes, which could result in a higher release of contaminants. A great difference was observed between diffusive and in situ benthic fluxes suggesting the enhancing of fluxes by bioturbation and the occurrence of biogeochemically important processes at the sediment–water interface. The multi-contamination of both inorganic and organic pollutants, in the sediments of the Mar Piccolo of Taranto (declared SIN in 1998), potentially transferable to the water column and to the aquatic trophic chain, is of serious concern for its ecological relevance, also considering the widespread fishing and mussel farming activities in the area.

The lakes’ fisheries play an important role in Egyptian economy. In 1980s, they provided more than 50 % of harvested fish in Egypt but now their contribution to the Egypt fish production decreased to only 12.5 % in 2012. Lake Timsah, one of the Suez Canal lakes, faced many challenges that lead to serious changes in its water and fish quality, fish production, as well as the catch composition. The present work investigated the impact of pollution, food availability, and excessive fishing mortality on the haffara production in lake Timsah. The distribution of four heavy metals (Pb, Zn, Ni, and Fe) was detected seasonally in water and in muscles, gills, and livers of Rhabdosargus haffara, during 2012 through 2013. Fe and Zn were presented by high values in liver, while Pb and Ni in gills. Generally, the lowest concentrations of all metals were found in muscles. The recorded crustacean organisms (the main food of haffara) decreased from 12 species and 32,079 organisms⁄m² in 2012 to only 7 species and 7290 organisms⁄m² in 2013 while the amphipods completely disappeared. This serious change was due to the severe pollution in the lake. A logistic surplus production model was fitted to the catch per unit effort indices, to estimate the maximum sustainable yield and the optimum level of fishing effort. The results revealed that haffara stock at lake Timsah is overfished, and the estimated precautionary target reference points advised the reduction of fishing effort by about 30–50 %.

The stereoselective cytotoxicity of new chiral acaricide etoxazole and its dissipation in citrus and soil were investigated for the first time. Enantioselective toxicity and oxidative stress of etoxazole toward MCF-7 cells was conducted. The phenomenon of dose- and form-dependent cytotoxicity was demonstrated by MTT and LDH assays, ROS generation, and SOD and CAT activity alternation. Cytotoxicity ranks were found to be consistent with oxidative damage as (R)- > Rac- > (S)-etoxazole. Moreover, the results of enantioselective degradation showed that (S)-etoxazole degraded faster than its antipode (R)-etoxazole. The gradual raise of EF values indicated the achievement of enantioselective degradation in citrus and soil, leaving the enrichment of (R)-etoxazole isomer. Significant differences of environmental behavior and cytotoxicity of etoxazole enantiomers were found in this study which provided valuable insight into the mechanism of potential toxicity and warranted more careful assessment of this pesticide before its agricultural application.

One hundred and eleven riverbed surface sediment (RSS) samples were collected to determine the heavy metal concentration throughout the Inner Mongolia reach of the Yellow River (IMYR), which has been subjected to rapid economic and industrial development over the past several decades. Comprehensive analysis of heavy metal contamination, including the enrichment factor, geo-accumulation index, contamination factor, pollution load index, risk index, principal component analysis (PCA), hierarchical cluster analysis (HCA), and Pearson correlation analysis, was performed. The results demonstrated that a low ecological risk with a moderate level of heavy metal contamination was present in the IMYR due to the risk index (RI) being less than 150 and the pollution load index (PLI) being above 1, and the averaged concentrations of Co, Cr, Cu, Mn, Ni, Ti, V, and Zn in the RSS, with standard deviations, were 144 ± 69, 77.91 ± 39.28, 22.95 ± 7.67, 596 ± 151, 28.50 ± 8.01, 3793 ± 487, 69.11 ± 18.44, and 50.19 ± 19.26 mg kg⁻¹, respectively. PCA, HCA, and Pearson correlation analysis revealed that most of the RSS was heavily contaminated with Zn, Ni, and Cu, due to the influence of anthropogenic activities; moderately contaminated with Ti, Mn, V and Cr because of the dual influence of anthropogenic activities and nature; and slightly to not contaminated with Co because it occurs mainly in the bordering desert areas. Graphic abstract ᅟ

A novel integrated system of anoxic-pure oxygen microbubble-activated sludge reactor-moving bed biofilm reactor was employed in treatment of real coal gasification wastewater. The results showed the integrated system had efficient performance of pollutants removal in short hydraulic retention time. While pure oxygen microbubble with the flow rate of 1.5 L/h and NaHCO₃ dosage ratio of 2:1 (amount NaHCO₃ to NH₄ ⁺-N ratio, mol: mol) were used, the removal efficiencies of COD, total phenols (TPh) and NH₄ ⁺-N reached 90, 95, and 95 %, respectively, with the influent loading rates of 3.4 kg COD/(m³ d), 0.81 kg TPh/(m³ d), and 0.28 kg NH₄ ⁺-N/(m³ d). With the recycle ratio of 300 %, the concentrations of NO₂ ⁻-N and NO₃ ⁻-N in effluent decreased to 12 and 59 mg/L, respectively. Meanwhile, pure oxygen microbubble significantly improved the enzymatic activities and affected the effluent organic compositions and reduced the foam expansion. Thus, the novel integrated system with efficient, stable, and economical advantages was suitable for engineering application.

The nitrite reductase (nirS and nirK) and nitrous oxide reductase-encoding (nosZ) genes of denitrifying populations present in an agricultural grassland soil were quantified using real-time polymerase chain reaction (PCR) assays. Samples from three separate pedological depths at the chosen site were investigated: horizon A (0–10 cm), horizon B (45–55 cm), and horizon C (120–130 cm). The effect of carbon addition (treatment 1, control; treatment 2, glucose-C; treatment 3, dissolved organic carbon (DOC)) on denitrifier gene abundance and N₂O and N₂ fluxes was determined. In general, denitrifier abundance correlated well with flux measurements; nirS was positively correlated with N₂O, and nosZ was positively correlated with N₂ (P < 0.03). Denitrifier gene copy concentrations per gram of soil (GCC) varied in response to carbon type amendment (P < 0.01). Denitrifier GCCs were high (ca. 10⁷) and the bac:nirK, bac:nirS, bac:nir ᵀ , and bac:nosZ ratios were low (ca. 10⁻¹/10) in horizon A in all three respective treatments. Glucose-C amendment favored partial denitrification, resulting in higher nir abundance and higher N₂O fluxes compared to the control. DOC amendment, by contrast, resulted in relatively higher nosZ abundance and N₂ emissions, thus favoring complete denitrification. We also noted soil depth directly affected bacterial, archaeal, and denitrifier abundance, possibly due to changes in soil carbon availability with depth.

Two bacterial strains, Achromobacter sp. (ACH01) and Sphingomonas sp. (SPH01), were isolated from a heavily polycyclic aromatic hydrocarbon (PAH)-contaminated soil (5431.3 ± 102.3 ppm) for their capacity to use a mixture of anthracene, pyrene, phenanthrene and fluorene as sole carbon sources for growth and for the capacity to produce biosurfactants. The two strains were exploited for bioaugmentation in a biopile pilot plant to increase the bioavailability and the degradation of the residual PAH contamination (99.5 ± 7.1 ppm) reached after 9 months of treatment. The denaturing gel gradient electrophoresis (DGGE) profile of the microbial ecology of the soil during the experimentation showed that the bioaugmentation approach was successful in terms of permanence of the two strains in the soil in treatment. The bioaugmentation of the two bacterial isolates positively correlated with the PAH depletion that reached 7.9 ± 2 ppm value in 2 months of treatment. The PAH depletion was assessed by the loss of the phyto-genotoxicity of soil elutriates on the model plant Vicia faba L., toxicological assessment adopted also to determine the minimum length of the decontamination process for obtaining both the depletion of the PAH contamination and the detoxification of the soil at the end of the process. The intermediate phases of the bioremediation process were the most significant in terms of toxicity, inducing genotoxic effects and selective DNA fragmentation in the stem cell niche of the root tip. The selective DNA fragmentation can be related to the selective induction of cell death of mutant stem cells that can compromise offsprings.