Declines of amphibian populations have been a worldwide issue of concern for the scientific community during the last several decades. Efforts are being carried out to elucidate factors related to this phenomenon. Among these factors, pathogens, climate change, and environmental pollution have been suggested as possible causes. Regarding environmental pollutants, some pesticides are persistent in the environment and capable of being transported long distances from their release point. In Costa Rica, some pesticides have been detected in protected areas, at locations where amphibian populations have declined. Information about toxicity of pesticides used in Costa Rican agriculture to amphibians is still scarce, particularly for native species.Toxicity tests with chlorothalonil, a fungicide intensively used in Costa Rica, were carried out exposing tadpoles of three Costa Rican native species: Agalychnis callidryas, Isthmohyla pseudopuma, and Smilisca baudinii in order to evaluate acute and chronic toxicity as well as the biomarkers cholinesterase activity (ChE), glutathione-S transferase activity (GST), and lipid peroxidation (LPO).96-h LC₅₀: 26.6 (18.9–35.8) μg/L to A. callidryas, 25.5 (21.3–29.7) μg/L to I pseudopuma and 32.3 (26.3–39.7) μg/L to S. baudinii were determined for chlorothalonil. These three species of anurans are among the most sensitive to chlorothalonil according to the literature. Besides, GST was induced in S. baudinii after exposure to sub-lethal concentrations of chlorothalonil while evisceration occurred in S. baudinii and A. callidryas tadpoles exposed to lethal concentrations of the fungicide. Chronic exposure to sub-lethal concentrations accelerated development in S. baudinii and caused lesions in tail of S. baudinii and I. pseudopuma tadpoles. Our results demonstrate that chlorothalonil is highly toxic to native amphibian species and that low concentrations can cause biochemical responses related to phase II of biotransformation and effects on development.

Wollastonite application in cadmium-contaminated soils can reduce cadmium concentrations in plant, while the side effect is the synchronous immobilization of micronutrients, which reduces micronutrient uptake in plant, inducing micronutrient deficient symptoms. Accordingly, we investigated whether the supplement of Zn and Mn fertilizers after the wollastonite addition could promote the growth and photosynthesis in amaranth (Amaranthus tricolor L.). In this study, plants were cultivated in cadmium-contaminated soil under micronutrient fertilization alone, wollastonite addition, and combination of wollastonite and micronutrient fertilization treatments. Then, plant biomass; photosynthesis parameters; and total Cd, Zn, and Mn concentrations were investigated. Moreover, chemical extractions were performed on soil samples. The results show that application of wollastonite decreased Cd, Zn, and Mn concentrations in plant and availability in soil and it increased the gas exchange ability of plants. But, it reduced the chlorophyll content in leaves and had no positive influence on plant biomass. In comparison, Zn and Mn fertilization after wollastonite application greatly increased plant biomass and photosynthetic ability. It also reduced Cd phytoavailability more efficiently. Therefore, synergistic improvement of physiological status of farmland crop by sequential treatment with first wollastonite for cadmium immobilization, and then micronutrient fertilization to avoid micronutrient deficiency, was demonstrated.

Composting facility leachate usually contains high concentrations of pollutants including heavy metals that are seriously harmful to the environment and public health. The main purpose of this study was to evaluate heavy metals removal from Isfahan composting facility (ICF) leachate by a horizontal flow constructed wetland (HFCWs) system. Two horizontal systems were constructed, one planted with vetiver and the other without plant as a control. They both operated at a flow rate of 24 L/day with a 5-day hydraulic retention time (HRT). The average removal efficiencies for Cr (53 %), Cd (40 %), Ni (35 %), Pb (30 %), Zn (35 %), and Cu (40 %) in vetiver constructed wetland were significantly higher than those of the control (P < 0.05). Accumulations of heavy metals in roots were higher than shoots. Cd and Zn showed the highest and the lowest bioconcentration factor (BCF), respectively. Vetiver tolerates the extreme condition in leachate including high total dissolved solids.

The effect of human activities on the presence of trace elements in the atmosphere was evaluated by analyzing samples of holm oak bark, collected in Italy in a large city, in a small town, and in a reference area, scarcely inhabited. In all cases, point sources of pollution were excluded (e.g., industries and incinerators). The concentrations of As, Cd, Co, Cu, Fe, Mn, Ni, Pb, V, and Zn were measured using inductively coupled plasma optical emission spectrometry (ICP-OES). The element concentrations in the small town are not different from the reference area, except for Pb and Cu, while the samples collected in the large city show higher concentrations of Co, Cu, Fe, Ni, Pb, V, and Zn with respect to the rural area. In particular, the Pb levels in the large city are approximately 16 times higher than in the reference site, and five times higher than in the small town. Most element concentrations are correlated in the large city, while in the reference site, only a few significant correlations between elements were found. Even in the absence of specific sources of pollution, populations living in big cities are exposed to higher concentrations of trace elements than those living in rural environments or in small urban centers.

Greywater (GW) is a potential source for water reuse in various applications. However, GW treatment is still a vital issue in water reuse in cases of environmental standards and risk to public health. This study investigates optimization and modeling of a hybrid process for COD removal from GW. Persulfate (PS) was simultaneously activated by electrogenerated ferrous ion (EC) and UV to generate sulfate radical. Photoelectro-persulfate (PEPS) was optimized by Box-Behnken design and the effects of four variables (pH, PS dosage, current density, and electrolysis time) were evaluated on COD removal. The results and several coefficients showed that the obtained model was acceptable for predicting the COD removal. Moreover, under optimum conditions (pH = 6.9, PS = 8.8 mM, current density = 2.0 mA/cm², and 49.3 min electrolysis time), BOD₅, turbidity, TSS, phosphate, and UV₂₅₄ were effectively removed and COD and BOD₅ values reached to discharge standards. Different configurations of the processes were assessed for COD removal. The order of COD removal efficiency followed: PS < Fe(II) < UV/PS ≤ Fe(II)/PS < Fe(II)/PS/UV < electrocoagulation ≤ electrocoagulation/UV < electro-PS < PEPS. The monitoring PS concentration during 60 min reaction time in the aforesaid processes indicated that PEPS could remarkably activate PS. The solution pH was also monitored and related results revealed that the presence of PS during the 10 min first time decreased pH value while production of hydroxide ion at cathode increased pH significantly. Finally, the contribution of electrochemical process in the electrical energy consumption was far less than that of photolysis process in hybrid PEPS process.

Toxins produced by cyanobacteria in freshwater ecosystems constitute a serious health risk worldwide for humans that may use the affected water bodies for recreation, drinking water, and/or irrigation. Cyanotoxins have also been deemed responsible for loss of animal life in many places around the world. This paper explores the effect of H₂O₂ treatments on cyanobacteria and microcystins in natural samples from a hypertrophic reservoir in microcosm experiments. According to the results, cyanobacteria were more easily affected by H₂O₂ than by other phytoplanktonic groups. This was shown by the increase in the fractions of chlorophyll-a (a proxy for phytoplankton) and chlorophyll-b (a proxy for green algae) over total phytoplankton pigments and the decrease in the fraction of phycocyanin (a proxy for cyanobacteria) over total phytoplankton pigments. Thus, while an overall increase in phytoplankton occurred, a preferential decrease in cyanobacteria was observed with H₂O₂ treatments over a few hours. Moreover, significant degradation of total microcystins was observed under H₂O₂ treatments, while more microcystins were degraded when UV radiation was used in combination with H₂O₂. The combination of H₂O₂ and ultraviolet (UV) treatment in natural samples resulted in total microcystin concentrations that were below the World Health Organization limit for safe consumption of drinking water of 1 μg/L. Although further investigation into the effects of H₂O₂ addition on ecosystem function must be performed, our results show that the application of H₂O₂ could be a promising method for the degradation of microcystins in reservoirs and the reduction of public health risks related to the occurrence of harmful algal blooms.

With the increasing use of municipal solid waste incineration (MSWI) and more stringent limits on landfilling of organic waste, more MSWI bottom ash is being landfilled, and the proportion of inorganic wastes in landfills is increasing, causing the increased Ca concentrations in landfill leachate. In this research, the inhibition effect of Ca concentration on the anaerobic treatment of landfill leachate was studied using a biochemical methane potential experiment. Slight inhibition of methane production occurred when the addition of Ca concentration was less than 2000 mg/L. When the addition of Ca concentration was between 6000 and 8000 mg/L, methane production was significantly reduced (to 29.4–34.8 % of that produced by the BLK reactor), and the lag phase was increased from 8.55 to 16.32 d. Moreover, when the dosage of Ca concentration increased from zero to 8000 mg/L, reductions in solution Ca concentration increased from 929 to 2611 mg/L, and the proportion of Ca in the residual sludge increased from 22.58 to 46.87 %. Based on the results, when the dosage of Ca concentration was less than 4000 mg/L, the formation of Ca precipitates on the surface of sludge appeared to prevent mass transfer and was the dominant reason for the reduction in methane production and sludge biomass. At higher Ca concentrations (6000–8000 mg/L), the severe inhibition of methane production appeared to be caused by the toxic effect of highly concentrated Ca on sludge as well as mass transfer blockage.

One hundred and eleven riverbed surface sediment (RSS) samples were collected to determine the heavy metal concentration throughout the Inner Mongolia reach of the Yellow River (IMYR), which has been subjected to rapid economic and industrial development over the past several decades. Comprehensive analysis of heavy metal contamination, including the enrichment factor, geo-accumulation index, contamination factor, pollution load index, risk index, principal component analysis (PCA), hierarchical cluster analysis (HCA), and Pearson correlation analysis, was performed. The results demonstrated that a low ecological risk with a moderate level of heavy metal contamination was present in the IMYR due to the risk index (RI) being less than 150 and the pollution load index (PLI) being above 1, and the averaged concentrations of Co, Cr, Cu, Mn, Ni, Ti, V, and Zn in the RSS, with standard deviations, were 144 ± 69, 77.91 ± 39.28, 22.95 ± 7.67, 596 ± 151, 28.50 ± 8.01, 3793 ± 487, 69.11 ± 18.44, and 50.19 ± 19.26 mg kg⁻¹, respectively. PCA, HCA, and Pearson correlation analysis revealed that most of the RSS was heavily contaminated with Zn, Ni, and Cu, due to the influence of anthropogenic activities; moderately contaminated with Ti, Mn, V and Cr because of the dual influence of anthropogenic activities and nature; and slightly to not contaminated with Co because it occurs mainly in the bordering desert areas. Graphic abstract ᅟ

Historical slags from the past Fe and Cu-Co production were investigated in order to evaluate either their potential for utilization or their long-term environmental risk for unsupervised old smelting areas. Here, we studied ferrous slags produced during the recovery of Fe from siderite-Cu ores in Slovakia and two different types of non-ferrous slags produced during the recovery of Cu and Co from Kupferschiefer ores in Germany. The glassy character, rare occurrence of primary silicate phases, and the lack of secondary phases in Cu slags indicate their stability for a prolonged period of time. Electron microprobe analytical work showed that the metals and metalloids (Cu, Co, Fe, Zn, Pb, As) are largely encased in droplets of matte and metal alloys and remain protected by the glassy matrix with its low weathering rate. Fe and Co slags are composed of high-temperature silicates such as wollastonite, cristobalite, as well as olivine, feldspar, quartz, leucite, pyroxene, and pyroxenoids. The presence of secondary phases attests to a certain degree metal release owing to weathering. Assuming minimal contents of metals in slags after a treatment with dilute H₂SO₄, slags could be used as pozzolanas for addition to cement.

Contaminant concentrations, sources, seasonal variation, and eco-toxicological risk of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) in surface water around Beijing from summer to winter in 2015 and 2016 were investigated. The concentrations of ∑OCPs and ∑PCBs ranged from 9.81 to 32.1 ng L⁻¹ (average 15.1 ± 7.78 ng L⁻¹) and from 7.41 to 54.5 ng L⁻¹ (average 21.3 ± 1.87 ng L⁻¹), respectively. Hexachlorocyclohexane (HCHs) were the dominated contamination both in aqueous and particulate phase. For PCBs, lower chlorinated PCBs were the major contaminants. Compositions of HCHs, dichlorodiphenyltrichloroethane (DDTs), and PCBs indicated that the sources of OCPs and PCBs in water were due to historical usage in the study areas. For OCPs, there was an obvious variation among three seasons, while insignificant change was shown for PCBs. Water quality standards for China’s surface water were not exceeded in this study. Concentrations at Miyun Reservoir, the primary source of drinking water to Beijing, when compared to the USEPA’s criterion for cancer risk was below the level of risk.