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Assessment of arsenic trioxide in the heart of Gallus gallus: alterations of oxidative damage parameters, inflammatory cytokines, and cardiac enzymes 全文
2017
Li, Si-Wen | Sun, Xiao | He, Ying | Guo, Ying | Zhao, Hong-Jing | Hou, Zhi-Jun | Xing, Ming-Wei
The aim of this study was to assess the effects of arsenic trioxide (As₂O₃) in the chicken heart, and 72 1-day-old male Hy-line chickens were fed either a commercial diet (C group) or an arsenic supplement diet containing 7.5 mg/kg (L group), 15 mg/kg (M group), or 30 mg/kg (H group) As₂O₃ for 90 days. The results showed that exposure to As₂O₃ merely lowered (P < 0.05) the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in M and H groups at 90 days, significantly downregulated the inhibition ability of hydroxyl radicals (OH·), and upregulated (P < 0.05) the contents of malondialdehyde (MDA) in As₂O₃ exposure groups at 30, 60, and 90 days. Meanwhile, the messenger RNA levels of inflammatory cytokines (tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and prostaglandin E synthase (PTGEs)) significantly increased (P < 0.05) in As₂O₃ exposure groups at 30, 60, and 90 days, and histological and ultrastructural damage was observed in As₂O₃ exposure groups. Additionally, As₂O₃-induced cardiac enzyme (aspartate transaminase (AST), creatine kinase (CK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and α-hydroxybutyrate dehydrogenase (α-HBDH)) levels increased (P < 0.05) at 90 days. These findings suggested that As₂O₃ exposure led to oxidative stress, inflammatory response, and histological and ultrastructural damage and altered the levels of cardiac enzymes in chicken heart tissues. This result may be helpful for further studies on the toxicological mechanisms of As₂O₃ in the chicken heart.
显示更多 [+] 显示较少 [-]Growth promoting potential of fresh and stored Moringa oleifera leaf extracts in improving seedling vigor, growth and productivity of wheat crop 全文
2017
K̲h̲ān, Shahbāz | Basra, Shahzad Maqsood Ahmed | Afzal, Irfan | Nawaz, Muhammad | Hafeez-ur-Rahman,
Wheat is staple food of region, as it contributes 60% of daily caloric intake, but its delayed sowing reduces yield due to short life span. Moringa leaf extract (MLE) is considered to improve growth and development of field crops. Study comprised of two experiments. First experiment, freshly extracted MLE and in combination with growth-promoting substances were stored at two temperature regimes. Chemical analysis, after 1, 2, and 3 months’ storage period, showed that phenolics and ascorbic acid concentrations decreased with increasing storage period. Fresh extracts improved speed and spread of emergence and seedling vigor. Effectiveness of MLE in terms of phenolics and ascorbate concentrations was highest up to 1 month which decreased with prolonged storage. Growth enhancing potential of MLE also reduced with increasing storage duration. Under field conditions, the bio-efficacy of these fresh and stored MLE was compared when applied as foliar spray at tillering and booting stages of wheat. Foliar applied fresh MLE was the most effective in improving growth parameters. Fresh MLE enhanced biochemical and yield attributes in late sown wheat. This growth-promoting potential of MLE decreased with storage time. Application of fresh MLE helped to achieve higher economic yield.
显示更多 [+] 显示较少 [-]Ant (Hymenoptera, Formicidae) diversity along a pollution gradient near the Middle Ural Copper Smelter, Russia 全文
2017
Belskaya, Elena | Gilev, Alexey | Belskii, Eugen
Ants are considered to be suitable indicators of ecological change and are widely used in land management and environmental monitoring. However, responses of ant communities to industrial pollution are less known so far. We studied pollution-related variations of ant diversity and abundance near the Middle Ural Copper Smelter (Russia) in 2009 and 2013, with pitfall traps set up at 10 sites in Picea obovata and Abies sibirica forest. This study provided evidences for humped pollution-induced dynamics of ant diversity and abundance. Species richness and diversity peaked in the habitat intermediate between slightly damaged and fully destroyed forest ecosystems. The total abundance of ants peaked in the middle of the pollution gradient and was determined mainly by the dominant species Formica aquilonia. The abundance of other species increased towards the smelter, but was less important for total abundance than that of red wood ants. Community dominants changed with increase of exposure; F. aquilonia, a typical species of mature forests, was replaced by species of open habitats, Lasius niger and Myrmica ruginodis. Habitat variables and competition between species seem to affect local ant communities more strongly than pollution exposure. Stand basal area and cover of the field layer were the main determinants of ant diversity and abundance of individual species.
显示更多 [+] 显示较少 [-]Temporal variability of ammonia emission potentials for six plant species in an evergreen subtropical forest in southwest China 全文
2017
Cui, Juan | Wang, Zhangwei | Zhang, Xiaoshan | Mulder, Jan | Zhang, Meigen
Temporal variability of ammonia emission potentials for six plant species in an evergreen subtropical forest in southwest China 全文
2017
Cui, Juan | Wang, Zhangwei | Zhang, Xiaoshan | Mulder, Jan | Zhang, Meigen
The temporal variability of leaf ammonia (NH₃) emission potentials (the ratio of leaf tissue ammonium to proton concentration) and nitrogen (N) pools of six dominant plant species were investigated at the Tieshanping (TSP) forested catchment, southwest China. The results showed that the NH₃ emission potentials and N pools presented small variations among seasons, which were mainly controlled by plant species and the leaf age. Also, high emission potential in one species did not correspond to high tissue N content. Specifically, the Chinese fir (Cunninghamia lanceolata) had higher NH₃ emission potential (mean: 46.2) but lower N content (mean: 1.6% of Dw). The leaf privet (Ligustrum quihoui Carr.) was with the moderate emission potential (15) and the highest N content (2.7% of Dw) on average, which for the Masson pine (Pinus massoniana) were both low. Overall, the emission potentials of the six species were too low (<200) to build up a sufficiently high NH₃ partial pressure in the leaves. Therefore, the Masson pine dominant subtropical forest at TSP acts as a sink for the atmospheric NH₃, indicating that using the N flux in throughfall only may significantly underestimate the N income of the ecosystem. The results are informative for future modeling of plant-atmosphere NH₃ exchange and estimating N budget in local or regional scales.
显示更多 [+] 显示较少 [-]Temporal variability of ammonium emission potentials for six plant species in an evergreen subtropical forest in Southwest China 全文
2017
Sorption and desorption of organophosphate esters with different hydrophobicity by soils 全文
2017
Cristale, Joyce | Álvarez-Martín, Alba | Rodríguez-Cruz, Sonia | Sánchez-Martín, María J. | Lacorte, Silvia
Organophosphate esters (OPEs) are ubiquitous contaminants with potentially hazardous effects on both the environment and human health. Knowledge about the soil sorption-desorption process of organic chemicals is important in order to understand their fate, mobility, and bioavailability, enabling an estimation to be made of possible risks to the environment and biota. The aim of this study was to use the batch equilibrium technique to evaluate the sorption-desorption behavior of seven OPEs (TCEP, TCPP, TBEP, TDCP, TBP, TPhP, and EHDP) in soils with distinctive characteristics (two unamended soils and a soil amended with sewage sludge). The equilibrium concentrations of the OPEs were determined by high performance liquid chromatography coupled to a triple quadrupole mass spectrometer (HPLC-MS/MS). All the compounds were sorbed by the soils, and soil organic carbon (OC) played an important role in this process. The sorption of the most soluble OPEs (TCEP, TCPP, and TBEP) depended on soil OC content, although desorption was ≥ 58.1%. The less water-soluble OPEs (TDCP, TBP, TPhP, and EHDP) recorded total sorption (100% for TPhP and EHDP) or very high sorption (≥ 34.9%) by all the soils and were not desorbed, which could be explained by their highly hydrophobic nature, as indicated by the logarithmic octanol/water partition coefficient (Kₒw) values higher than 3.8, resulting in a high affinity for soil OC. The results of the sorption-desorption of the OPEs by soils with different characteristics highlighted the influence of these compounds’ physicochemical properties and the content and nature of soil OC in this process.
显示更多 [+] 显示较少 [-]Pilot testing model to uncover industrial symbiosis in Brazilian industrial clusters 全文
2017
Saraceni, Adriana Valélia | Resende, Luis Mauricio | de Andrade Júnior, Pedro Paulo | Pontes, Joseane
The main objective of this study was to create a pilot model to uncover industrial symbiosis practices in Brazilian industrial clusters. For this purpose, a systematic revision was conducted in journals selected from two categories of the ISI Web of Knowledge: Engineering, Environmental and Engineering, Industrial. After an in-depth revision of literature, results allowed the creation of an analysis structure. A methodology based on fuzzy logic was applied and used to attribute the weights of industrial symbiosis variables. It was thus possible to extract the intensity indicators of the interrelations required to analyse the development level of each correlation between the variables. Determination of variables and their weights initially resulted in a framework for the theory of industrial symbiosis assessments. Research results allowed the creation of a pilot model that could precisely identify the loopholes or development levels in each sphere. Ontology charts for data analysis were also generated. This study contributes to science by presenting the foundations for building an instrument that enables application and compilation of the pilot model, in order to identify opportunity to symbiotic development, which derives from “uncovering” existing symbioses.
显示更多 [+] 显示较少 [-]Treatment of municipal wastewater in full-scale on-site sand filter reduces BOD efficiently but does not reach requirements for nitrogen and phosphorus removal 全文
2017
Laaksonen, Petteri | Sinkkonen, Aki | Zaitsev, Gennadi | Mäkinen, Esa | Grönroos, Timo | Romantschuk, Martin
A traditional sand filter for treatment of household wastewater was constructed in the fall of 2012 at Biolinja 12, Turku, Finland. Construction work was led and monitored by an authorized wastewater treatment consultant. The filter was placed on a field bordered by open ditches from all sides in order to collect excess rain and snowmelt waters. The filter was constructed and insulated from the environment so that all outflowing water was accounted for. Untreated, mainly municipal, wastewater from Varissuo suburb was pumped from a sewer separately via three septic tanks (volume = 1 m³ each) into the filters. Normally, wastewater was distributed to ground filters automatically according to pre-programmed schedule. Initially, the daily flow was 1200 L day⁻¹ to reflect the average organic load of a household of five persons (load: ca 237 g day⁻¹ BOD; 73 g day⁻¹ total N; and 10.4 g day⁻¹ total P). Later in the test, the flow rate was decreased first to 900 and then to 600 L day⁻¹ to better reflect the average volume produced by five persons. Volumes of inlet wastewater as well as treated water were monitored by magnetic flow meters. Samples were withdrawn from the inlet water, from the water entering the filters after the third septic tank, and from the outflowing water. After an initial adaption time, the reductions in BOD and chemical oxygen demand were constantly between 92 and 98%, showing that the biological degradation process in the filters functioned optimally and clearly comply with the national and EU standards. The reduction in total nitrogen and total phosphorus, however, reached required levels only during the first months of testing, apparently when buildup of microbial biomass was still ongoing. After this initial period of 3 months showing satisfactory reduction levels, the reduction of total nitrogen varied between 5 and 25% and total phosphorus mostly between 50 and 65%. Nitrification was efficient in the filter, but as indicated by high nitrate levels and poor nitrogen reductions, denitrification was inefficient or absent. During the winter period, the temperature in the filter dropped to near freezing, but at all time points, the flow of water was unaffected by freezing. During snowmelt and heavy rain, occasional flooding was observed. Such situations may lead to dilution rather than purification of the wastewater. In conclusion, the sand filter tested worked well for reduction of the organic load in municipal wastewater but failed to sufficiently reduce nitrogen and phosphorus levels.
显示更多 [+] 显示较少 [-]A preliminary investigation of noise impact within metro stations in the urban city of Guangzhou, China 全文
2017
Wang, Peng | Wang, Yimin | Zou, Chao | Guo, Jixing
The noise in the metro station is synthesized from a variety of different noise sources. Excessive noise exposure will bring serious impacts on humans’ health. To alleviate the shortage of the urban land, most metro stations are planning to develop convenience stores and shopping malls. In order to evaluate the potential noise impact on humans’ comfort in the metro stations, this study selected four representative stations of Guangzhou Metro Line 1 to carry out a preliminary questionnaire survey of 226 respondents for the perception and perform a noise measurement. Additionally, platform screen doors as a potential method for noise mitigation were examined. The results show that the noise caused by trains was dominant in the metro stations; however, the noise impact by public broadcast and passengers cannot be ignored. The noise levels of the transfer stations were obviously greater than the normal stations, especially during the peak hours. In addition, people in the metro stations have potential exposure to noise that the noise levels exceed the criteria limit of China, which would bring discomfort for humans; however, the normal activities of people would not be impacted. The platform screen doors should be further improved or relevant noise mitigation methods should be taken into account to reduce the noise level within the criteria limit.
显示更多 [+] 显示较少 [-]Enhancement of Chlorella vulgaris harvesting via the electro-coagulation-flotation (ECF) method 全文
2017
Wong, Y. K. | Ho, Y. H. | Leung, H.-M. | Ho, Dominic K. C. | Yau, Y. H. | Yung, K. K. L.
This article explores the potential of using an electro-coagulation-flotation (ECF) harvester to allow flotation of microalgae cells for surface harvesting. A response surface methodology (RSM) model was used to optimize ECF harvesting by adjusting electrode plate material, electrode plate number, charge of the electrodes, electrolyte concentration, and pH value of the culture solution. The result revealed that three aluminum electrode plates (one anode and two cathodes), brine solution (8 g/L), and acidity (pH = 4) of culture solution (optimized ECF harvester) The highest flocculant concentration was measured at 2966 mg/L after 60 min and showed a 79.8 % increase of flocculation concentration. Such results can provide a basis for designing a large-scale microalgae harvester for commercial use in the future.
显示更多 [+] 显示较少 [-]The effects of lead stress on photosynthetic function and chloroplast ultrastructure of Robinia pseudoacacia seedlings 全文
2017
Zhou, Jian | Jiang, Zeping | Ma, Jie | Yang, Lifeng | Wei, Yuan
In this experiment, the effects of different lead (Pb) concentrations (0, 200, 600, 1000, 1400 mg kg⁻¹) on photosynthesis and chlorophyll fluorescence in Robinia pseudoacacia seedlings were examined. As Pb concentration increased, chlorophyll a, chlorophyll b, total chlorophyll content, net photosynthetic rate, transpiration rate, stomatal conductance (g ₛ), and mesophyll intercellular carbon dioxide concentration were gradually reduced. Maximal photochemical efficiency, photochemical quenching, and quantum yield also decreased. However, the initial fluorescence and nonphotochemical quenching gradually increased. Chloroplasts swelled owing to local plasmolysis and lost most of their starch content, and their thylakoid lamellae gradually became disordered and loosely packed. When the chloroplast envelope was lost under high Pb stress (≥1000 mg kg⁻¹), lipid globules were released into the surrounding mesophyll cell. Multiple regression analysis showed that g ₛ and inactivity of the PSII reaction center had the greatest effect on photosynthetic function, whereas inhibition of electron transport had minimal effects on black locust seedlings under Pb stress.
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