Evaluation of the human exposure risk to semivolatile organic compound (SVOC) levels in soil from automobile workshops in Awka was investigated. Soil samples were collected in both dry and rainy seasons. Solvent extraction of the soil samples was carried out using n-hexane: acetone mixture (1:1). Concentrations of SVOCs were determined using gas chromatography-mass spectrometry. There were higher concentrations of SVOCs in the dry season than in the rainy season. The concentrations of the SVOCs were compared with standards for industrial soils. Concentrations of pentachlorophenol in the samples for dry and rainy seasons were below the Canadian Council of Ministers of Environment (CCME) acceptable limit of 7.6 mg/kg. Eighty percent of soil samples for the dry season and all the soil samples for the rainy season had benzo(a)pyrene concentrations lower than the CCME acceptable limit of 0.7 mg/kg. However, incremental lifetime cancer risk (ILCRdₑᵣ) of PAHs and pentachlorophenol for dry seasons exceeded 1.0 × 10⁻⁶ WHO acceptable limit in all the sampling stations, which indicates potential risk via dermal contact. ILCRs of pentachlorophenol were above 1.0 × 10⁻⁶ in 60% of the samples for soil ingestion and all the samples for dermal contact. Hazard quotient of phenolics, phthalates, 1,3-dichlorobenzene and 1,4-dichlorobenzene for soil samples were less than 1 for both seasons, which indicates no non-cancer risk. Results suggest that the SVOCs were highest at the centre of the automobile workshop and the main route of exposure was dermal contact with the soil.

This study evaluated the effects of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on the physical growth, intestinal histomorphology, and blood metabolites in Salmonella-challenged birds during the finisher phase. In this study, a total of 600 chicks (Ross 308) were randomly distributed into the following dietary treatments: basal diet with no treatment; infected with Salmonella; T1, infected + avilamycin; T2, infected + Bacillus subtilis; T3, Saccharomyces boulardii; T4, infected + oregano; T5, infected + calcium montmorillonite. Our results indicated that feed consumption, body weight gain, total body weight, and feed conversion ratio increased significantly (P < 0.01) in T1 and T2. Villus width increased significantly (P < 0.01) in T1 while the total area was significantly (P < 0.01) higher in T1 and T2 among the treatment groups. Blood protein was significantly (P < 0.01) high in T3 and T4; however, the glucose concentration was significantly (P < 0.01) high in T2, T3, and T4. The treatments increased significantly (P< 0.01) in the treatment groups compared to the negative control. Aspirate aminotransferase (AST) was significantly (P < 0.05) low in T3 compared to the positive control. In conclusion, the results indicated that supplementation of Bacillus subtilis and calcium montmorillonite improved the production performance compared to other feed additives in broiler chicks infected with Salmonella during the finisher phase.

Magnetic Fe₃O₄ assembled on nanoscale zero-valent iron (nZVI) supported on an activated carbon fiber (ACF) to form nanoscale magnetic composites (nZVI-Fe₃O₄/ACF) for removing Cr(VI) and Cu(II) from aqueous solution through a permeable reactive column was synthesized via an in situ reduction method. The nZVI-Fe₃O₄/ACF composites and the interaction between nZVI-Fe₃O₄/ACF and both Cr and Cu ions were characterized by field emission scanning electron microscopy (FESEM) with EDX, TEM, XRD, and XPS. Batch experiments were used to analyze the effects of main factors on Cr(VI) removal and investigate the simultaneous removal of Cr(VI) and Cu(II) through a permeable reactive column. The results indicated that the ACF and Fe₃O₄ can inhibit the agglomeration and enhance the dispersibility of nZVI, and Fe₃O₄ and nZVI displayed good synergetic effects. The removal efficiency of Cr(VI) improved with the increase amount of Fe₃O₄ in the nZVI-Fe₃O₄/ACF composites. With low initial concentration of Cr(VI) and acidic conditions, ~ 90% of 20.0 mg·L⁻¹ Cr(VI) in the solution was removed after 60 min. The removal of Cr(VI) was also affected by coexisting ions. The removal efficiency of 10.0 mg·L⁻¹ Cu(II) was ~ 100% after 45 min of reaction, and the presence of Cu(II) can accelerate the reduction of Cr(VI). The simultaneous removal mechanisms of Cr(VI) and Cu(II) by the nZVI-Fe₃O₄/ACF composites also were proposed.

Engineered nanoparticles (NP) like Ag and TiO₂ offer unique properties for various applications. Thus, the entry of the NP in soil environments is expected to increase in the future due to their growing industrial use. To avoid potential hazards due to these anthropogenic products, NP behavior in the environment should be well understood. In natural soil solutions, we investigated NOM adsorption onto Ag and TiO₂ NP and its influence on NP colloidal stability. Therefore, we extracted soil solutions from a floodplain soil (Fluvisol) and a farmland soil (Cambisol) differing in NOM quality and inorganic ion concentration. We measured the amount of adsorbed organic carbon as well as changes in aromaticity and molecular weight of NOM upon adsorption onto NP. Additionally, the size and zeta potential of NP in both soil solutions were investigated. We observed that the highly hydrophilic NOM of floodplain soil solution rich in inorganic ions strongly adsorbed to Ag but not to TiO₂ NP. Instead, sorption to TiO₂ NP was observed for the more hydrophobic NOM of the farmland soil with low ionic strength which did not sorb to Ag NP. These differences had a strong effect on NP stability, leading to Ag NP destabilization in case of floodplain soil solution and TiO₂ NP stabilization in the presence of farmland soil solution. Our results point out the necessity of studies in more complex systems and suppose that oxic and metallic NP might show very different fate depending on the environment they are exposed to.

Forest soils (mainly soil organic carbon) play an important role in the retention of nitrogen and mercury, and loss of the forest floor during wildfires can stimulate N and Hg losses. In this paper, we investigate long-term impacts of forest fire on soil N and Hg concentrations at Acadia National Park (ANP) in Maine. Acadia National Park experienced a severe fire in 1947. Within the national park, Hadlock Brook watershed was left unburned, whereas most of Cadillac Brook watershed was intensely burned, with substantial loss of the forest floor. Post-fire regeneration in Cadillac was mostly as hardwood species, whereas vegetation in Hadlock remained predominantly softwood. We sampled soils in both watersheds in 2015, approximately 70 years after the fire. The soils were analyzed for total carbon (TC), total nitrogen (TN), total mercury (THg), and methylmercury (MeHg) content. Compared to Hadlock, Cadillac soils had ~ 50% lower TC, ~ 40% lower TN, and ~ 50% lower THg content, reflecting the loss of forest floor 70 years ago. Methylmercury concentrations in Cadillac were approximately 2 times the concentrations in Hadlock, indicating that conditions were more conducive to methylation, potentially due to differences in forest type. Long-term comparisons of stream DOC, NO₃⁻, and THg concentrations between the two watersheds demonstrated that concentrations were significantly lower in Cadillac Brook, reflecting greater retention in Cadillac and a legacy of lower atmospheric deposition in the hardwood as compared to softwood watershed. This study provides insights on the multi-decadal recovery from a stand-replacing disturbance and underscores the persistence of altered soil biogeochemistry.

In this work, hexavalent chromium adsorption onto LDPE and agave fiber composites coated with chitosan or cellulose was studied in batch experiments. Chemical modifications consisting in cross-linked chitosan, cross-linked chitosan xanthate, and cellulose xanthate were applied to the polysaccharide-coated sorbents in order to increase their stability and adsorption capacity. The sorbents were characterized in terms of morphology by scanning electron microscopy and their chemical composition was evaluated by infrared and nuclear magnetic resonance spectroscopies. The results showed that the adsorption kinetics followed the pseudo-second-order model in all cases (i.e., chemisorption as the rate-limiting step of the adsorption reaction). Moreover, the isotherms evidenced a monolayer adsorption on homogeneous sites described by the Langmuir model. The maximum adsorption capacity of 284.7 mg Cr(VI)/g was obtained for the cross-linked chitosan xanthate sorbent at pH 4 which represents an increase of 43% against the chitosan-coated sorbent (199.1 mg Cr(VI)/g). Besides, functionalized cellulose sorbent also increased its capacity from 84.5 to 106.0 mg Cr(VI)/g cellulose due to the xanthate group. Up to six adsorption-desorption cycles were completed for the case of functionalized chitosan sorbent, confirming that the stability was increased with the cross-linking and the material could be reused several times without losing its adsorption capacity. In the case of cellulose xanthate, only three adsorption cycles were completed. However, improvements were observed in the desorption capacity considering that it decreased below 20% after two cycles in the cellulose-coated sorbent.

Unanticipated increase in the use of silver (Ag) and copper oxide (CuO) nanoparticles (NPs) due to their antimicrobial properties is eliciting environmental health concern because of their coexistence in the aquatic environment. Therefore, we investigated the genetic and systemic toxicity of the individual NPs and their mixture (1:1) using the piscine micronucleus (MN) assay, haematological, histopathological (skin, gills and liver) and hepatic oxidative stress analyses [malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT)] in the African mud catfish, Clarias gariepinus. The fish were exposed to sublethal concentrations (6.25–100.00 mg/L) of each NP and their mixture for 28 days. Both NPs and their mixture induced significant (p < 0.05) increase in MN frequency and other nuclear abnormalities. There was significant decrease in haemoglobin concentration, red and white blood cell counts. Histopathological lesions observed include epidermal skin cells and gill lamellae hyperplasia and necrosis of hepatocytes. The levels of MDA, GSH and activities of SOD and CAT were impacted in C. gariepinus liver following the exposure to the NPs and their mixture. Interaction factor analysis of data indicates antagonistic genotoxicity and oxidative damage of the NPs mixture. These results suggest cytogenotoxic effects of Ag NPs, CuO NPs and their mixture via oxidative stress in Clarias gariepinus.

This work studies the in situ electrobioremediation of an oxyfluorfen-polluted clay soil in a two-stage method. First, a fixed-bed biofilm reactor for oxyfluorfen biodegradation in wastewater was developed; it treated wastewater with 200 mg L⁻¹ of oxyfluorfen and reached 100% of oxyfluorfen degradation in 30 h. Second, a portion of the biofilm-covered bed was included into the polluted soil and it was used as a biological permeable reactive barrier (BioPRB), whereas electrokinetics was applied to promote the contact between the pollutant and microorganisms into the soil. The electrobioremediation study was performed in a bench scale setup under 1.0 V cm⁻¹ at room temperature and under periodic polarity reversal (2 day⁻¹) in a 2-week batch experiment. Two reference tests were done: (i) a conventional in situ biological test without electrokinetics and (ii) a conventional in situ electrokinetic test without using microorganisms. The experimental conditions (temperature, pH, moisture) were correctly controlled in the soil and enabled the microbial activity during the process. A low oxyfluorfen removal efficiency was obtained after 2 weeks (11%) because of the low electrokinetic mobility of such non-polar pollutant into the soil. Despite this low efficiency value, it was considered that the combined biological-electrokinetic technology could be used as a bioaugmentation procedure to perform electrobioremediation processes because the results of both reference tests showed negligible removal efficiencies when using only biological or only electrochemical methods. According to these results, electrobioremediation could be considered a feasible technology although more retention time would be required to achieve successful remediation results.

Experiments were conducted under lead (Pb), cadmium (Cd), and copper (Cu) exposure to observe germination and seedling growth of wheat (Triticum aestivum L), pea (Pisum sativum), and tomato (Solanum lycopersicum L.). Metals were applied in five concentrations (20, 65, 110, 175, and 220 ppm) and Hoagland solution was used to feed the seedlings. Irrespective of the tested crop seeds, copper revealed maximum effect (51.2%) on germination followed by lead (47.5%) and cadmium (35.3%). Tomato seeds were most sensitive in germination stage followed by pea and wheat. In seedling stage, tomato also showed highest sensitivity to both Cd and Cu. However, pea seedlings showed higher tolerance to Pb and wheat seedlings had the highest tolerance to both Cu and Cd. Toxicity and tolerance of metals was found to vary with crops and growth stages. Higher transfer of metals (Pb, Cd, and Cu) in wheat seedling indicates higher risk of food chain contamination when grown in polluted soil. Higher mobility and uptake of Cd in tomato and wheat seedlings even under lower concentration of exposure needs further study.

The purpose of this study is to experimentally investigate the effect of unsaturation of the biodiesels obtained from grapeseed oil, wheat germ oil and coconut oil (reference fuel) for compression ignition (CI) engine application. Fatty acid profile analysis and physio-chemical properties were determined by standard test procedures. Engine testing was carried out in a 5.2-kW single-cylinder CI engine and the combustion, performance and emission characteristics were analysed. The effect of fuel property variation and the combustion reaction kinetics due to unsaturation difference have been discussed. The maximum brake thermal efficiency at full load for diesel was found to be 32.3% followed by 31.3%, 30.2% and 27.4 %, respectively, for coconut biodiesel (CBD), grapeseed biodiesel (GSBD) and wheat germ biodiesel (WGBD). Maximum heat release rate as observed for diesel, CBD, GSBD and WGBD are 63.2 J/°CA 60.7 J/°CA and 59 J/°CA and 43.4 J/°CA respectively. The brake-specific NO emission at full load is higher for CBD followed by GSBD, WGBD and diesel having values of 9.23 g/kWh, 8.91 g/kWh, 8.21 g/kWh and 7.6 g/kWh respectively. Conversely, the smoke emission is lower for CBD compared to the other tested fuels.