The world’s interest now is focusing on the applying of the principles of sustainable development in managing natural resources, especially in managing freshwater, which is one of the greatest challenges the whole world face. In this paper, the illite ore was tested to remove the excess of iron and manganese from groundwater which is used for drinking and household purposes in Assiut. To accomplish this goal, the study was based on two directions. The first direction focused on achieving a physicochemical analytical survey for all the groundwater produced by wells in Assiut governorate, and its averages are plotted on GIS maps, to illustrate the difference in the concentrations of iron and manganese. However, the second direction focused on the preparation and examination of the modified illite ore, and it was characterized by using XRD, SEM, FT-IR, and XRF techniques. The results showed that the sodalite-bearing modified illite (SBMI) was able to remove the excess of iron and manganese from raw groundwater with the efficiency of 99% and 97%, respectively.

The considerable effect of enzymes on human health draws great attention to enzyme-based drugs (therapeutic enzymes), in recent times. L-asparaginase (ASNase) is a well-known therapeutic enzyme. It has varied applications and is a single molecule for the treatment of multiple diseases. This study tries to extract asparaginase from soybean debris (agricultural wastes) as a cheap plant source and compare this with microbial asparaginase as an agent in cancer chemotherapy. The asparaginase was extracted and purified from soybean debris (plant asparaginase) and Pseudomonas aeruginosa (microbial asparaginase), then the physiochemical characters were determined for the two enzymes, and the anticancer activity of plant and microbial asparaginase was determined against gastric cancer (CLS-145), pancreatic cancer (AsPC-1), colon cancer (HCT116), esophagus cancer (KYSE-410), liver cancer (HepG2), breast cancer (MCF-7), and cervical cancer (HELLA). The results showed that plant asparaginase was superior to microbial asparaginase in its physiochemical characters. Plant asparaginase showed higher stability and activity under the conditions of changing either the temperature or the pH; also plant asparaginase has a higher affinity to the asparagine than the microbial asparaginase; besides, this plant asparaginase did not show activity with glutamine as a substrate. The plant asparaginase showed higher anticancer activity than that of microbial asparaginase against all studied cancer cell lines. The present study introduces as the first time a comparative study between the plant and microbial asparaginase which proves that soybean debris asparaginase can be more efficient and safe than that of the microbial asparaginase as an anticancer agent.

There is growing pressure to find technically feasible and economically viable solutions in reducing emissions of pollutants from various occupational settings to minimise environmental pollution. Hence, it is essential to develop and test methods for controlling pollutants from occupational backgrounds. We have tested an air cleaner device in reducing aerosol numbers by filtration and airborne bacteria by photocatalysis from an enclosed type dairy barn. Here, we had shown a significant reduction of larger size aerosol numbers (2.0–10.0 µm) and airborne total aerobic bacteria and Staphylococcus aureus (S. aureus) and complete clearance of Escherichia coli (E. coli) in the exhaust air of the air cleaner device. A greater 8.05% and 61.56% reduction of 5.0–10.0 µm aerosol numbers and airborne E. coli, respectively, were observed in the instantly treated central air of the dairy barn. We had found an increasing trend of aerosol numbers and airborne bacteria concentrations in the central air of the dairy barn after stopping the air cleaner device. We also had observed increased bacterial load in the filter paper of the air treatment chamber of the air cleaner device with the advancement of cleaning time. These findings are essential to validate air cleanings from various types of dairy microenvironments.

Recent environmental research has found that people with higher incomes and in more developed countries are more willing to pay (WTP) to protect their environment than people in developing countries. Based on this assumption, the study investigated Pakistani citizens’ attitudes toward environmental protection, precisely their willingness to pay higher prices and taxes to preserve the natural environment. The research was carried out in three Punjab cities (Hasan Abdal, Wah, and Taxila) and four KPK cities (Abbottabad, Havelian, Mansehra, and Haripur). The selected cities are home to knowledgeable people who work in various universities, schools, hospitals, medical colleges, and nearby industrial estates and have a sense of environmental protection and can understand the healthcare issues related to environmental damages. The survey was divided into two sections: one about the participants’ socio-demographic information and the other about people’s willingness to pay higher prices and taxes to protect the environment. Four hundred and sixty-two people took part in the survey, and the data were analyzed using the bootstrap regression approach. The results show that gender has a detrimental impact, although population density and education positively impact a country’s willingness to pay for environmental protection (WTPEP). Women are more likely to engage in pro-environmental behavior than men, resulting in disparities in their perceptions of male and female respondents in the study. People who live in crowded places tend to pay for environmental protection because of population density, healthcare difficulties, and air pollution. The respondents are well-versed in the externalities of environmental pollution; they are hopeful about paying for a better healthcare environment. Other criteria, such as the respondent’s income, health status, total pollution level in the country, and per capita income, enable respondents to pay for environmental preservation to achieve long-term sustainable growth. The government must embrace air quality regulations and empower its citizens by offering better healthcare services since they are enthusiastic about paying higher taxes and fees to protect the environment.

Heavy metals contamination in sediments may endanger ecosystems and human health via the food chain. In fact, there is little to no understanding about heavy metal accumulation in surface sediment of one of the most economically important marine bodies for Vietnam, the Thai Binh Coast, where five large rivers co-discharge into the Gulf of Tonkin. Twenty-seven surface sediment samples were collected from the intertidal regions and analyzed for: arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), and zinc (Zn) using inductively coupled plasma mass spectroscopy (ICP-MS). The studied area exhibited a large spatial variation in the concentration of heavy metals, e.g., the dry sediment concentration of Cd was the least (0.05–0.49 mg.kg⁻¹), whereas that of Zn was the greatest (45.4–252 mg.kg⁻¹). Based on the geoaccumulation index ([Formula: see text]), most of the studied heavy metals were accumulated at low pollution levels, except four locations exhibited moderately and highly polluted levels of Hg with [Formula: see text] Hg values from 1.92 to 2.66. However, the high contamination factor value implicated that not only Hg but also all other detected heavy metals in this area resulted from anthropogenic activities along the coast and the river upstream. This implied the need for quick action from the government. In addition, numerous analytical methods were used to see the association between metals, total organic carbon (TOC), and particle size distribution, including Pearson correlation coefficient (P) and principal component analysis (PCA). Hg demonstrates lowest connection with TOC (PHg₋TOC ~ 0) but individual heavy metal correlations are largely positive, with many reaching 1.0 (e.g., PNᵢ₋Cᵣ = 0.89, PCd₋Aₛ = 0.72, PNᵢ₋Cᵤ = 0.76, and PCᵤ₋Cᵣ = 0.72). From the PCA diagram, we can observe that those sampling points in the positive direction of PC1 were expected to have a high concentration of Cu, Zn, As, Ni while having extremely little sand content.

The application of low-carbon alcohols (LCA fuels) in internal combustion engines has become one of the most important topics in road transport decarbonization. This paper aims to identify the trends and characteristics of LCA combustion research for the period 2000–2021 through bibliometric analysis. Citation analysis is used to evaluate the influence of most productive journals, countries/regions, authors, institutions, and relevant literature, while collaborative network between various authors, countries/regions, institutions, and the co-occurrences among different keywords are discussed. A dataset of 2250 publications was extracted from the Web of Science Core database and analyzed with CiteSpace and Biblioshiny. The extracted documents involve 429 journals of publications by 4782 authors from 1434 institutions across 83 countries/regions. The results reveal that the research output in this field has undergone three main stages of development, i.e., initial development (2000–2007), slow development (2008–2015), and rapid development (2016–2021). Currently, the research field is growing at an annual growth rate of 9.24%, with most of the contributions by authors and institutions originating from China. The analysis from relevant keywords and literature suggests that the core of this research field centers on the combustion, performance, and emission characteristics of LCA-fueled engines. The current study helps keep the scientific community informed of the latest paradigms in the LCA combustion research field.

The world faces a high alert of coronavirus disease 2019 (COVID-19), leading to a million deaths and could become infected to reach a billion numbers. A sizeable amount of scholarly work has been available on different aspects of social-economic and environmental factors. At the same time, many of these studies found the linear (direct) causation between the stated factors. In many cases, the direct relationship is not apparent. The world is unsure about the possible determining factors of the COVID-19 pandemic, which need to be known through conducting nonlinearity (indirect) relationships, which caused the pandemic crisis. The study examined the nonlinear relationship between COVID-19 cases and carbon damages, managing financial development, renewable energy consumption, and innovative capability in a cross section of 65 countries. The results show that inbound foreign direct investment first increases and later decreases because of the increasing coronavirus cases. Further, the rise and fall in the research and development expenditures and population density exhibits increasing coronavirus cases across countries. The continued economic growth initial decreases later increase by adopting standardized operating procedures to contain coronavirus disease. The inter-temporal relationship shows that green energy source and carbon damages would likely influence the coronavirus cases with a variance of 17.127% and 5.440%, respectively, over a time horizon. The policymakers should be carefully designing sustainable healthcare policies, as the cost of carbon emissions leads to severe healthcare issues, which are likely to get exposed to contagious diseases, including COVID-19. The sustainable policy instruments, including renewable fuels in industrial production, advancement in cleaner production technologies, the imposition of carbon taxes on dirty production, and environmental certifications, are a few possible remedies that achieve healthcare sustainability agenda globally.

The integration of Industry 4.0 (I4.0) has emerged as an innovative paradigm for industrial firms contemplating environmental and economic issues. This study explicates the role of I4.0 technologies (I4.0TEC) in reinforcing the management of environmental assets (ENVASS) as well as optimizing financial performance (FP). The data in this research was collected from 738 industrial firms in Malaysia between 2009 and 2018. The analyses of ordinary least square statistics (OLS) and structural equation modeling (SEM) delineated three major findings. The individual effect of ENVASS, robotization, and flexibility in production technologies has a marginal impact on sales, exports, and labor productivity indicators. The complementarities of these variables represent a similar effect on the performance indicators. The findings related to gross operating margin elucidate that ENVASS and I4.0TEC have neither individual nor complementarity effects. This was explained by developing a robust model by integrating ENVASS, I4.0TEC, spending and investing in R&D, flexibility in production, and human capital management. Our findings have confirmed that the proposed model offers a functional toolkit for the firms considering optimizing their profitability by leveraging ENVASS and I4.0TEC. This research also contributes to developing an ethical business model for the circular economy.

Current experiment was planned to investigate the deleterious effects of the graded doses of aflatoxin B1 (AFB1) on white leghorn male birds. For this purpose, one-hundred birds of 8 weeks of age were divided into 4 equal groups and reared on feed contaminated with different doses of AFB1 for 10 weeks. Group A was kept as a control group and was fed with normal toxin-free diet; groups B, C, and D were offered feed containing 100 ppb, 200 ppb, and 400 ppb of AFB1, respectively. The birds were euthanized at the 4th and 10th week of the experiment. Clinical signs, behavioral changes, absolute and relative organ weight of the testes, and sperm motility were measured. Cellular immune response was observed through carbon clearance assay (CCA), P-HAP, and antibody response against sheep red blood cells (SRBC). Results showed a dose-dependent decline in the immune response of birds with the increase in the level of AFB1 in the feed. A significant decrease in the serum levels of testosterone, prolactin, and LH were observed at the end of the study. Grossly, testicular size and volume were reduced in ABF1 fed birds, while histological examination showed moderate to severe necrosis of testicular parenchyma, with partial to complete arrest of spermatogenesis. Very few spermatozoa were found in group C, while they were almost absent in group D which was offered a diet containing 400 ppb AFB1. The motility of sperms was reduced in all treated groups except control. The abovementioned results showed that AFB1 had severe toxic effects on the reproductive and immunological parameters of WLH male birds in a dose-dependent manner.

The concept of green development has gradually penetrated into the enterprise. Green mergers and acquisitions (M&A) have gradually become a means for heavily polluting enterprises to achieve the goal of energy conservation and emission reduction and embark on the path of green transformation. Heavily polluting enterprises have acquired clean technology and resources through green M&A, and whether they will promote their green innovations after green M&A has not yet been explored. Based on the data of M&As of China’s heavy polluting enterprises from 2010 to 2018, this study empirically tests whether the M&As of heavy polluting enterprises can promote green innovation. The results show that M&As by heavily polluting enterprises can promote green innovation, and this impact is promoted with the support of government subsidies. In addition, older or higher paid CEOs negatively moderate this effect. Therefore, our study believes that most of the M&As of heavy polluting enterprises are taking the initiative to take environmental protection responsibilities and embarking on the path of green transformation. The government can issue relevant policies to encourage heavily polluting enterprises to conduct green M&A in order to achieve their goal of green transformation. Our study has enriched the relevant literature on green investment and green innovation, and can be used as a reference for the government to introduce policies for the green transformation of heavily polluting enterprises.