During the period 1990-1997, the content of 11 trace metals (Fe, Cr, Co, Al, Mn, Cd, Cu, Ni, Pb, Zn, Sr) was studied in muscle and other organs of fish from DTD (Danube-Tisza-Danube) channel system (Yugoslavia). According to obtained and analysed data, the trace metal bioaccumulation can possibly act as enviromental indicator in biomonitoring. DTD (Danube-Tisza-Danube) Channel System can, therefore, be classified as the 3rd class, concerning analysed trace metal content.

The determination of main and some biogenic and trace elements in the sea water of the Boka Kotorska Bay (Adriatic sea, Montenegro, Yugoslavia) was carried out by the atomic apsorption and emission spectrometric method (FAAS) and by the hydride technique AAS (HGAAS).

Biosystematic research of fish population from the river Nisava (Serbia, Yugoslavia) is placed from the mouth of Jerma river and Nisava river to the mouth of Nisava river and Juzna Morava river (Serbia, Yugoslavia). The next part of the research is about determining the methodology for counting of particular fish species in the river Nisava. Hematological analyses of some species were done at the same time in order to establish the relation between water quality and number of erythrocytes, leucocytes and quantity of haemoglobin. Two autochthonous and allochthonous species were analyzed. The method used in this research are explained as well.

During the period from 11th May, 1991 to 5th March, 1994, seasonal study of the river Jablanica (Serbia, Yugoslavia) macrozoobenthos was carried out. Investigations were done of three sectors: in the upper, middle and down reach of the river, with the aim to estimate water quality changes by the changes in bottom fauna composition. Bottom fauna community was composed of 17 invertebrate groups with 100 taxa determined. The most diverse Oligochaeta (21 taxa) and Ephemeroptera (19 taxa). Macroinvertebrates diversity decreased downstream the river as a result of the pollutional degree increase. Saprobic indices were from 1.51 (sector 2) to 1.8 (sector 3).

The paper presents some procedures for identification of low flow required for taking preventive measures in the rivers with no comprehensive hydrological observations. Besides using time - space parameters, temparery or simultaneous hydrometric observations in the observed rivers are also recommended. The paper concludes with the presentation of relevant low flow calculation results for one river in Zlatibor region (Serbia, Yugoslavia), which has not been examined so far.

During the 1981-1996 period, qualitative and quantitative structure of oligachaeta communities as indicator of the eutrophication process was investigated in the lake Palic (the 2nd oxidation lake), Serbia (Yugoslavia). The mean annual number of oligochaetes was related to nutrient concentration (total nitrogen and total phosphorus). The analysed data for both oligochaete community and nutrients, leads to the conclusion that the second oxidation lake Palic can be classified as an highly eutrophic environment, with tendency towards dystrophic, particularly in past two years.

Competing ions strongly affect heavy metal sorption onto the solid surfaces of soil. This study evaluated competitive sorption of Cd, Cu, Ni, Pb and Zn on three soils: Calcixerollic Xerochrept, Paralithic Xerorthent and Lithic Haplumbrept. Monometal and competitive sorption isotherms were obtained at 25°C. The individual effect of ions on retention of the others was ascertained by a fractional factorial analysis design. Most of the sorption isotherms belonged to type L subtype 2 in the classification of Giles. In competitive sorption the initial linear part was shorter and the knee sharper when compared with monometal sorption isotherms. Parameters related to sorptive capacity, such as Point B, Langmuir monolayer and Freundlich distribution coefficient, were higher in monometal than in competitive sorption, and in basic soils than in acidic soil. Calcium desorbed at different points of the sorption isotherms indicated that cationic exchange with Ca was the main retention mechanism in calcareous soils. For Pb, the ratio Ca desorbed/Pb sorbed was close to one; for Cu, Ni and Zn the ratio ranged from 1.20 to 1.37, probably due to partial dissolution of calcium carbonates by hydrolytic processes during retention. On the other hand, Cd had a ratio around 0.6 reflecting another additional retention mechanism, probably surface complexation. Fractional factorial design confirmed that the presence of the cations investigated reduced the amount of the five metals retained, but the presence of Cu and Pb in the system depressed Ni, Cd and Zn sorption more than the inverse. Cation mobility was enhanced when equilibrium concentration increased and the effect was higher in Ca-saturated soils.

International audience | This review summarizes how salinity and temperature, two key global factors driven by climate change in freshwater systems, interact with other stressors on organisms in controlled small-scale factorial experiments at the population, individual, or subindividual level (excluding mesocosm and field studies). Despite the growing interest, research following all these criteria remains limited with 156 publications of which 50% analyzed stressors + salinity, 46% stressors + temperature, and only 4% involved the triple combination. Research on the combined effect of temperature and salinity predominantly focused on metals, pesticides, and, to a lesser extent, emergent contaminants, such as microplastics and nanomaterials, encompassing various biological models and responses. In general, increased temperature amplifies the single effect of stressors, whereas salinity leads to a higher diversity of responses, with similar proportions of synergisms and antagonisms. Fish (Salmoniformes, Perciformes, and Cypriniformes) were the most studied organisms. Among Crustacea, only cladocerans of the genera Daphnia and Ceriodpahnia were considered. The present review highlights the need to include other species that play key roles in freshwater food webs and to increase triple combination studies to understand complex interactions and develop adaptation and mitigation strategies to preserve the environment and its services in this changing world.

International audience | Antimicrobial resistance (AMR) is a burgeoning environmental concern demanding a comprehensive One Health investigation to thwart its transmission to animals and humans, ensuring food safety. Seafood, housing bacterial AMR, poses a direct threat to consumer health, amplifying the risk of hospitalization, invasive infections, and death due to compromised antimicrobial treatments. The associated antimicrobial resistance genes (ARGs) in diverse marine species can amass and transmit through various pathways, including surface contact, respiration, and feeding within food webs. Our research, focused on the English Channel and North Sea, pivotal economic areas, specifically explores the occurrence of four proposed AMR indicator genes (tet(A), blaTEM, sul1, and intI1) in a benthic food web. Analyzing 350 flatfish samples' skin, gills, and gut, our quantitative PCR (qPCR) results disclosed an overall prevalence of 71.4% for AMR indicator genes. Notably, sul1 and intI1 genes exhibited higher detection in fish skin, reaching a prevalence of 47.5%, compared to gills and gut samples. Proximity to major European ports (Le Havre, Dunkirk, Rotterdam) correlated with increased AMR gene frequencies in fish, suggesting these ports' potential role in AMR spread in marine environments. We observed a broad dispersion of indicator genes in the English Channel and the North Sea, influenced by sea currents, maritime traffic, and flatfish movements. In conclusion, sul1 and intI1 genes emerge as robust indicators of AMR contamination in the marine environment, evident in seawater and species representing a benthic food web. Further studies are imperative to delineate marine species' role in accumulating and transmitting AMR to humans via seafood consumption. This research sheds light on the urgent need for a concerted effort in comprehending and mitigating AMR risks in marine ecosystems within the context of One Health.