Aeration by airflow technology is a reliable method to accelerate waste biodegradation and stabilization and hence shorten the aftercare period of a landfill. To simulate hydro-biochemical behaviors in this type of landfills, this study develops a model coupling multi-phase flow, multi-component transport and aerobic-anaerobic biodegradation using a computational fluid dynamics (CFD) method. The uniqueness of the model is that it can well describe the evolution of aerobic zone, anaerobic zone, and temperature during aeration and evaluate aeration efficiency considering aerobic and anaerobic biodegradation processes. After being verified using existing in situ and laboratory test results, the model is then employed to reveal the bio-stable zone development, aerobic biochemical reactions around vertical well (VW), and anaerobic reactions away from VW. With an increase in the initial organic matter content (0.1 to 0.4), the bio-stable zone expands at a decreasing speed but with all the horizontal ranges larger than 17 m after an intermittent aeration for 1000 days. When waste intrinsic permeability is equal or greater than 10⁻¹¹ m², aeration using a low pressure between 4 and 8 kPa is appropriate. The aeration efficiency would be underestimated if anaerobic biodegradation is neglected because products of anaerobic biodegradation would be oxidized more easily. A horizontal spacing of 17 m is suggested for aeration VWs with a vertical spacing of 10 m for screens. Since a lower aeration frequency can give greater aeration efficiency, a 20-day aeration/20-day leachate recirculation scenario is recommended considering the maximum temperature over a reasonable range. For wet landfills with low temperature, the proportion of aeration can be increased to 0.67 (20-day aeration/10-day leachate recirculation) or an even higher value.

Zn stress seriously induces various toxic responses in Withania somnifera L., when accumulated above the threshold level which was confirmed by investigating the responses of protein, expression of antioxidant enzymes, and elemental profiling on accumulation of Zn. Zn was supplemented in the form of ZnSO₄ (0, 25, 50, 100, and 200 μM) through MS liquid medium and allowed to grow the in vitro germinated plants for 7 and 14 days. The study revealed that when the application of Zn increased, a significant reduction of growth characteristics was noticed with alterations of proteins (both disappearance and de novo synthesis). The activity of CAT, SOD, and GPX were increased up to certain concentrations and then declined, which confirmed through in-gel activity under different treatments. RT-PCR was conducted by taking three sets of genes from CAT (RsCat, Catalase1, Cat1) and SOD (SodCp, TaSOD1.2, MnSOD) and found that gene RsCat from CAT and MnSOD from SOD have shown maximum expression of desired genes under Zn stress, which indicate plant’s stress tolerance mechanisms. The proton-induced X-ray emission study confirmed an increasing order of uptake of Zn in plants by suppressing and expressing other elemental constituents which cause metal homeostasis. This study provides insights into molecular mechanisms associated with Zn causing toxicity to plants; however, cellular and subcellular studies are essential to explore molecule-molecule interaction during Zn stress in plants.

The environmental fate of iodine is of general geochemical interest as well as of substantial concern in the context of nuclear waste repositories and reprocessing plants. Soils, and in particular soil organic matter (SOM), are known to play a major role in retaining and storing iodine. Therefore, we investigated iodide and iodate sorption by four different reference soils for contact times up to 30 days. Selective sequential extractions and X-ray absorption spectroscopy (XAS) were used to characterize binding behavior to different soil components, and the oxidation state and local structure of iodine. For iodide, sorption was fast with 73 to 96% being sorbed within the first 24 h, whereas iodate sorption increased from 11–41% to 62–85% after 30 days. The organic fraction contained most of the adsorbed iodide and iodate. XAS revealed a rapid change of iodide into organically bound iodine when exposed to soil, while iodate did not change its speciation. Migration behavior of both iodine species has to be considered as iodide appears to be the less mobile species due to fast binding to SOM, but with the potential risk of mobilization when oxidized to iodate.

The associations between exposure to short-term ambient air pollution and daily atherosclerotic heart disease (ASHD) mortality in cool climate have not been established. We performed a time-series analysis in Shenyang, the largest city of Northeastern China. We identified 7659 ASHD deaths and obtained deaths, ambient air pollution levels, and meteorological data for Shenyang during 2014–2017. The impact of ambient air pollution on daily ASHD deaths was analyzed using generalized additive models (GAMs). Cumulative lag effects were investigated using distributed lag non-linear models (DLNM). We found ASHD deaths significantly increased during days with higher air pollution. Particulate matter with diameter < 2.5 μm (PM₂.₅), PM₁₀, and sulfur dioxide (SO₂) were positively associated with ASHD deaths among the total population. Both single- and multi-pollutants models indicated that PM₂.₅, PM₁₀, and sulfur dioxide (SO₂) were positively associated with the deaths of women with AHSD, whereas only SO₂ was significant in men. This suggests significant gender-based differences in the fatal effects of ambient air pollution. Up to 28 days of single-day lag effects were observed for PM₂.₅ and PM₁₀ in women. The cumulative lag effects of PM₂.₅ and PM₁₀ showed increasing trends in both men and women; however, exposure to higher pollutant concentrations did not necessarily translate to greater risks. The ERRs differences between women and men were larger in cold days than in hot days, suggesting that lower temperature may exacerbate the adverse effects of air pollution on vulnerable women.

While it is well-known that the toxicity of mercury for plants is related to its bioavailability in the environment in which the plant lives, few studies have addressed Hg effects under controlled conditions of life-limiting available Hg concentrations. This study examines the effects of Hg on the holm oak (Quercus ilex L.) exposed to medium-high available Hg concentrations. Holm oak seeds were sown in a perlite substrate and grown in the presence of a nutrient solution containing 0, 5, 25, or 50 μM Hg. The variables determined as outcome measures were impacts on germination, growth, and nutrient accumulation along with Hg concentration in leaves, stems, and roots at different growth stages. Our findings suggest no overall detrimental effects of the metal on germination, nutrient accumulation, and plant growth, although root morphology was clearly modified. Mercury accumulation in the plant varied according to time, organ, Hg treatment dose, and plant growth stage. When comparing Hg build-up in the different organs, highest concentrations of the metal were detected in the roots, followed by the leaves and stems. The Hg accumulation pattern was positively correlated with time and Hg dose, whereas negative correlation was observed with growth stage. The impacts of all these factors on Hg accumulation were not additive pointing to interesting interaction effects that should be explored in future work.

Cyanate (CNO⁻) has been produced in the environment through either natural or anthropogenic sources. However, due to industrialization, it has been led more over-loads. In this study, interaction of CNO⁻ uptake by rice seedlings with nitrate assimilation was investigated using gene expression analysis after an acute phytotoxicity assay. Our results showed that CNO⁻ exposure caused inhibition on relative growth rates of plants. CNO⁻ analysis demonstrated that rice seedlings had higher potential for CNO⁻ uptake and the removal rates showed a zero-order kinetic. PCR analysis exposed that OsCYN transcript was not significantly induced by CNO⁻ treatments in rice tissues and CNO⁻ exposure also repressed gene expression of the collaborative enzyme carbonic anhydrase (CA), suggesting that assimilation of CNO⁻ initiated by the enzyme cyanase (CYN) in rice seedlings was an enzyme-limitation reaction. Gene expression of other enzymes involved in nitrate metabolism was tissue-specific under CNO⁻ exposure, suggesting that rice seedlings were able to trigger its intrinsic regulative and responsive mechanisms to cope up with uneven N conditions. Significant upregulation of three OsGDH isogenes, except for OsGDH1 in roots, was detected in both rice materials with enhancing CNO⁻ concentrations, suggesting that GDH may play a primary role to maintain the balance of C and N in plants under CNO⁻ exposure. In conclusion, because the innate pool of CYN activity was non-sufficient to degrade exogenous CNO⁻ by rice seedlings, CNO-derived ammonium only can serve as a supporting N source to support growth of rice seedling under non-effective doses of CNO⁻ exposure.

We measured the concentrations of acid volatile sulfide (AVS), chemical oxygen demand (COD), and metals (As, Cd, Cu, Hg, Pb, and Zn) in surface sediments of 74 intertidal sectors along the western and southern coasts of Korea to determine their spatial distribution and pollution status. The concentrations of AVS, COD, and metals were relatively higher in northwestern and southeastern coasts due to human and industrial activities around metropolitan, industrial complexes, and large-scale farms. The results of the sediment quality guidelines and geoaccumulation index for organic matter and metal revealed that almost all intertidal sediments were unpolluted with regard to AVS, COD, Cd, Cu, Hg, Pb, and Zn and some intertidal sediments in northwestern and southeastern coasts were moderately polluted with regard to As. However, the results of pollution load index and ecological risk index for metal showed that intertidal sediments in the southeastern coast are significantly polluted and could have an adverse effect on benthic organisms. Thus, the appropriate management policy and restoration plan for intertidal sectors with high metal pollution level in Korea is necessary to improve the quality of intertidal sediment.

The immobilizing effects of wood biochar (BW2%) and iron grit (Z1%) applied alone or in combination (BW2% + Z1%) to agricultural (M750) and brownfield (MAZ) soils highly contaminated by metals were assessed in a greenhouse experiment. The results showed that Z1% and BW2% + Z1% were the most efficient amendments to reduce Cd, Cu, Pb, and Zn mobility, environmental availability, and phytoavailability in the M750 soil. The oxidation of Z1% allowed part of the Cu and Zn pools present in exchangeable or carbonate-bound forms (labile fraction) to complex in less mobile forms. In this soil, the metal chemical extractions (0.01 M CaCl₂ and 0.05 M EDTA) and the DGT (diffusive gradient in thin films) devices to assess metal in soil solution and soil pore water (SPW) also highlighted the immobilizing characteristic of Z1%. In most cases, the addition of BW2% to Z1% (BW2% + Z1%) did not improve this effect, except for the dissolved Pb and Zn concentrations in the M750 soil solution. It was also observed that Cd, Pb, and Zn passed throughout DGT mimicking the biological cell membrane were reduced by all amendments of the M750 soil corroborating metal concentrations measured in rye grass shoots. In the MAZ soil, metals were less available as shown by their low extractability rate, low capacity of metal resupply from the solid phase to pore water, and low phytoavailability. The poor metal availability could be explained by the high levels of carbonate and organic matter contents in this soil. Nevertheless, a decrease of the Cu environmental availability and the Cu concentrations in rye grass shoots grown on the MAZ soil was also observed in the soil amended with Z1% alone or in combination with BW2%. From a health point of view, the most effective amendment to reduce human exposure through ingestion of soil particles for the M750 and MAZ soils was BW2% for Cd and BW2% + Z1% for Pb. However, the presence of rye grass minimized the amendments’ beneficial effects.

To assess the two most toxicologically relevant species of As, namely arsenite (As(III)) and arsenate (As(V)), chromatographic separations often require two separate chromatographic columns to address the co-elution of arsenobetaine (AsB) with As(III). This issue is typically observed using conventional isocratic methods on anion exchange columns, increasing cost and analysis time. Here, we optimize the extraction of inorganic As from a lichen air biomonitor and develop an isocratic method for the chromatographic separation of five common As species on a PRP X-100 anion exchange column, resulting in the complete baseline separation of all species under study. This method was then applied to lichen biomonitors from an urban and rural site to demonstrate its use. In order of abundance, the various arsenic species in lichens from the urban site in South Africa were As(V) > As(III) > AsB > dimethylarsinic acid (DMA) > monomethylarsonic acid (MMA), and As(V) > AsB > As(III) > DMA > MMA for the rural site, where MMA was present in extremely low, non-quantifiable concentrations in lichens from both sites. Total concentrations of As were higher in samples from the urban site (6.43 ± 0.25 μg/g) than in those from the rural site (1.87 ± 0.05 μg/g), with an overall extraction efficiency of 19% and 40%, respectively. The optimized method utilized relatively inexpensive solvents and is therefore low-cost and eco-friendly in comparison with conventional chromatographic techniques. This is the first study which addresses the optimized extraction and characterization of As species in a South African lichen biomonitor of air pollution. Graphical abstract .

There are a limited number of studies on the estimation of environmental Kuznets curve (EKC) hypothesis for nitrous oxide (N₂O) emissions, though it is one of the most harmful greenhouse gases (GHGs) present in ambient atmosphere. In the wake of industrialization, it is necessary to understand the impact of energy consumption pattern on N₂O emissions and revise the energy policies accordingly. In this study, we have analyzed the impact of renewable and fossil fuel energy consumptions on N₂O emissions for APEC countries over the period of 1990–2015, and the analysis has been carried out following the EKC hypothesis framework. The results obtained from the study indicate the efficacy of the renewable energy solutions in having positive impact on environmental quality by helping to reduce the level of N₂O emissions. The policy implications derived from the results are designed while keeping the objectives of sustainable development goals (SDGs) in mind, so that the energy policies can bring forth sustainability in the economic systems in these nations.