This study evaluated the effect of applying biofertilizer in the soil on the cultivation of corn. Different doses of biofertilizer associated with chemical fertilizer were applied in the soil to meet the plants’ nutritional demand. Four months after sowing, plant samples were collected and evaluated, by measuring the height and productivity of biomass, dry matter, neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and nitrogen (N). Soil samples were also collected to measure the concentrations of macronutrients, base saturation, and exchangeable acidity. The biofertilizer application did not alter the levels of dry matter, NDF, ADF, CP, and N in the plants, or the concentrations of nitrogen, phosphorus, magnesium, and copper in the soil. Besides this, the largest average values of the plant heights and levels of potassium in the soil were found in the treatment with the highest biofertilizer dose. Notably, there was a significant increase in the quantity of fresh and dry matter in the treatments that received biofertilizer in comparison with the controls. The results obtained indicated the potential of substituting conventional fertilization with fertirrigation using biofertilizer, an alternative procedure that can help reduce the environmental impacts caused by dairy farming, regarding the release of wastewater into watercourses.

Recently, analytical procedures based on computer vision related to the colorimetric analysis of digital images have been described in the scientific literature. In this sense, a novel analytical approach is presented based on digital image colorimetry for nickel determination. The method consists in the development of a system with solid-phase extraction, consisting basically of an extraction chamber filled with polystyrene divinylbenzene sorbent impregnated with the complexing reagent 1-(2-thiazolylazo)-p-cresol (TAC) and a portable microscope multifunction, used for image acquisition. The image of the sorbent after extraction of Ni is obtained. This image is related to the concentration of the elements. The variables (pH, flow, and sample volume) were evaluated using a full factorial design 2³ for the screening and a Doehlert matrix to establish the significant variables’ optimal levels. The enrichment factor and limit of detection were 148 and 0.8 μg L⁻¹, respectively. The method was applied to the determination of nickel in river water, coffee, and cigarette samples.

The results in the article have both practical and scientific significance. The practical significance is due to the need to determine the parameters of the initial condition of natural landscapes in the impact zones (IZ) of the separated parts of launch vehicles, which will be used to identify changes in the process of their operation. Scientific significance is associated with the low knowledge of the studied landscapes, because of their inaccessibility. The main components of the landscape, which may be adversely affected by space rocket activity (surface water, snow cover, plants, and soils), were used as the objects of the study. Samples were taken in three stages: autumn, winter, and summer-autumn. During the autumn stage, water samples were taken; during the winter, snow samples; and during the summer-autumn, water, soil, and plant samples. Acidity (pH), chemical oxygen demand (COD), hydrocarbons, and ionic composition were determined in water and snow samples; pH, hydrocarbons and total organic carbon, elemental composition, and cation exchange capacity (CEC) were determined in soil samples; elemental composition was determined in plant samples. During the autumn low-water season, the chemical properties of water in all rivers did not differ significantly, except for Junguele river, which had a lower acidity and more intense chemical oxygen demand, as well as a higher concentration of Mg²⁺ and K⁺ ions. In the winter stage, it was revealed that the snow cover of all planned IZ will turn into slightly acidic or neutral waters with a slight concentration of hydrocarbons, NO₃⁻, Mg²⁺, Zn²⁺, Mn²⁺, and Al³⁺, the distribution of which is uniform within the boundaries of each IZ. During the summer-autumn stage, despite the flood, no significant changes in the chemical composition of water in the studied rivers were detected.

Mining industry generates large volumes of waste known as mine tailings, which contain heavy metals (HMs) that generate a risk to environmental health. Thus, remediation of HM pollution requires attention. In this study, HM bioaccumulation, genotoxic damage, and morphological and physiological changes in the tree species Prosopis laevigata were evaluated in order to assess its potential for remediation of mine tailings. P. laevigata plants were established in two treatments (reference substrate and tailing substrate) under greenhouse conditions. Every 2 months, six individuals were selected per treatment for 1 year. From each individual, macromorphological (height, stem diameter, and number of leaves), micromorphological (stomatal coverage and stomatal index), and physiological parameters (chlorophyll content) were evaluated, as well as the concentration of Pb, Cu, Cd, Cr, Fe, and Zn in root and foliar tissue. Genetic damage was assessed by the comet assay in foliar tissue. These parameters were evaluated in adult individuals established in mine tailings. Roots bioaccumulated significantly more HM compared to foliar tissue. However, the bioaccumulation pattern in both tissues was Fe > Pb > Zn > Cu. The plants in tailing substrate reduced significantly the morphological and physiological characters throughout the experiment. Only the bioaccumulation of Pb affected significantly the levels of genetic damage and the number of leaves, while Zn reduced plant height. The percentage of plants that have translocation factor values greater than 1 are Cu (92.9) > Fe (85.7) > Pb (75.0) > Zn (64.3). P. laevigata has potential to phytoremediate environments contaminated with metals, due to its dominance and establishment in abandoned mine tailings, and its ability to bioaccumulate HM unaffecting plant development, as well as their high levels of HM translocation.

Water contamination with cadmium (Cd) is a global environmental problem and its remediation becomes urgent. Phytoremediation using ornamental plants has attracted much attention for its advantages of cost-effectiveness and beautification of the environment. Nelumbo nucifera G. is a popular ornamental aquatic macrophyte with fast growth, large biomass, and high capacities for Cd accumulation and removal. However, information about Cd resistance and defense responses in N. nucifera is rather scarce, which restricts its large-scale utilization for phytoremediation. The phytohormone ethylene plays an important role in plant resistance to Cd stress, but the underlying mechanism remains unclear. In this study, we investigated morphophysiological responses of N. nucifera seedlings to Cd stress, and focused on the effects of ethylene on oxidative damage, Cd accumulation, and antioxidant defense system at the metabolic and transcript levels in leaves under Cd stress. Our results showed that Cd exposure led to leaf chlorosis and necrosis, coupled with an increase in contents of hydrogen peroxide, electrolyte leakage, and malondialdehyde, and decrease in chlorophyll content. Exogenous ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) application intensified Cd-induced stress responses and Cd accumulation, and increased ethylene production by inducing ACC synthase (ACS) gene NnACS. Such enhanced ethylene emission inhibited catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) activities, and modulated ascorbate (AsA) and glutathione (GSH) accumulation through transcriptional regulation of their respective metabolic genes. After ethylene action inhibitor silver thiosulfate (STS) supplementation, Cd-induced oxidative damage was abolished, and Cd content declined but still at a relatively high level. Blocking of ethylene perception by STS inhibited ethylene biosynthesis; enhanced CAT, APX, and GR activities and their transcript levels; increased AsA accumulation via inducing its biosynthetic genes; but reduced GSH content and NnGSH2 expression level. These results suggest that ethylene biosynthesis and signaling play an important role in N. nucifera response to Cd stress, and maintaining appropriate ethylene level and low ethylene sensitivity could improve its Cd tolerance via efficient antioxidant defenses.

The present work is focused on the removal of NOx with reduced blue TiO₂ with Fe (blue Fe-TiO₂)- and Cu (blue Cu-TiO₂)-doped photocatalyst. TiO₂ was reduced via lithium in EDA (blue TiO₂). Fe and Cu ions were doped in the reduced TiO₂ (blue Fe-TiO₂ and blue Cu-TiO₂). The material resulted in a core-shell structure of amorphous and anatase phase. XPS suggests the existence of Ti³⁺ species and oxygen vacancies within the structure of TiO₂. Additionally, valence bond (VB)-XPS shows the generation of intermediate levels at the band edge of the doped photocatalyst. Photocurrent, electrochemical impedance spectroscopy and cyclic voltammetry confirmed the enhanced charge-separation process in doped reduced TiO₂. The photocatalysts were tested for the photo-oxidation of NOx. Blue Fe-TiO₂ reveals the efficiency of 70% for NO elimination and 44.74% for NO₂ formation. The improved efficiency of the doped photocatalyst is related to the re-engineered structure with Ti³⁺ species, oxygen vacancies, and charge traps. Electron spin resonance (ESR) measurement was carried out for blue Fe-TiO₂ to confirm the formation of reactive oxygen species (ROS). Furthermore, ion chromatography was used to investigate the mechanism of NOx oxidation. In conclusion, the doped blue TiO₂ has a strong tendency to photo-oxidize NOx gasses.

Floating vegetated islands (FVIs) are extensively implemented in various river ecology restoration projects, given their capability of decontaminating pollutants. The fluid dynamical behaviors of turbulence through FVIs are studied in the flume by using the SonTek Acoustic Doppler Velocimetry. Through conventional spectral and quadrant analyses, flow characteristics, such as energy content and turbulent momentum exchange, are investigated as the flow encountered a series of root canopies. A shear layer with corresponding coherent vortex structures at the bottom of root canopies occurred, which is generated by Kelvin–Helmholtz instabilities. These instabilities are usually derived from velocity differences between root canopy and gap region. Shear- and stem-scale vortices are identified by using spectral analysis. The power spectral density function on measured vertical velocity fluctuations in the flow direction near the bottom of root canopies from the leading edge of FVIs is computed. Given the flow developing downstream, a series of the spectral curves has gradually showed one dominant dimensionless frequency at 0.046. The sweep and ejection events have contributed prominently to the Reynolds stress in whole vertical direction. Momentum flux carried by sweeps outweighs its counterpart carried by ejections inside root canopies. However, the situation is different outside root canopies. The sweep–ejection contributions are brief but crucial to the total turbulent momentum exchange, which is in good agreement with considerable studies on turbulent flow through canopies.

Soil fungi have been widely studied, but the effects of heavy metal contamination at various levels as well as the abundance and diversity of heavy metal tolerant fungi in the contaminated paddy soils are still unknown. The purpose of this study is to analyze the adaptability of fungi at different levels of heavy metal contamination to identify species that have strong adaptability to heavy metals. In this research, the technology of high-throughput sequencing was applied to study fungal communities in severe level (SL), moderate level (ML), light level (LL), and clean level (CL) for soil samples polluted by heavy metal, as well as to analyze the relations between environmental variables and fungal communities. The spearman analysis showed that 6 dominant fungal phyla and 18 dominant fungal genera were significantly correlated with these environmental variables. The α-diversity indexes of the soil fungal community from SL, ML, and CL were, mostly, drastically higher than the LL samples (p < 0.05). Meanwhile, Ascomycota, the main fungal phylum, was spotted to yield a strong tolerance towards heavy metals, especially in ML. The most dominant genera of tolerant fungi in this area, which are Aspergillus, Penicillium, and Fusarium, could absorb and transport the heavy metals with the help of nutrients under certain heavy metal contamination levels. Therefore, this study indicated that some fungi, which have strong biodegradability on heavy metals, can reduce toxicity of heavy metals and create a proper soil environment to grow food crops. Graphical abstract

The coal-to-electricity project (CTEP) using electricity instead of coal for heating is a significant measure to cope with climate change and air pollution in China. After years of development, the CTEP has been implemented on a large scale of areas in Beijing. An evaluation model is proposed in this paper to analyze the environmental benefits and assist in determining whether the CTEP should be continued. Firstly, the heating system and framework of this paper are introduced. We also analyzed the feasibility of CTEP, illuminating how electric heating benefits the environment. Secondly, the evaluations models of CTEP are built, including reduction of coal, pollutant emission, and overall benefit. Finally, based on the models results are obtained and discussed and prove environmental benefits of CTEP. It is calculated that 18 kg of coal per unit heating area can be saved in the CTEP, which effectively reduces 98% of PM2.5, 95% of SO₂, 44% of NOX, and 81% of CO₂ emissions. The CTEP has increasing contribution to the reductions of atmospheric pollutants. The considerable environmental benefits demonstrate the significance of the CTEP in the north of China, which also facilitate its future development in areas outside of Beijing.

Repeated deposition of copper oxide nanoparticles (CuO-NPs) into aquatic systems makes them a global threat since the NPs accumulate in various organs of the fish particularly skeletal muscle. In the present study, adult zebrafish were exposed to different concentrations of CuO-NPs (1 and 3 mg/L) for a period of 30 days. The status of functional markers (acetylcholinesterase, creatine kinase-MB, and lactate dehydrogenase) and oxidative stress markers (oxidants and antioxidants) were analyzed. The histological changes in muscle were studied followed by the immunohistochemistry expression for catalase. Further, the expression of myoD, myogenin, pax7, β-actin, and desmin was examined by semi-quantitative reverse transcriptase polymerase chain reaction. The results indicated that chronic exposure to CuO-NPs causes muscular damage as evidenced by elevated levels of functional markers. There was a significant increase in the oxidants with reduction in the antioxidant levels, implying that the antioxidant enzymes were unable to scavenge the free radicals induced by the CuO-NPs. The histopathological analysis showed degeneration and atrophy in the treated groups confirming muscle damage. The immunohistochemical catalase expression in the muscle was reduced in the treated groups further supporting the evidence that the antioxidant has suffered a decline. The altered gene expression indicates skeletal muscle damage due to the CuO-NPs exposure. Overall, the data suggest that chronic exposure to CuO-NPs caused muscular toxicity which may lead to muscle degeneration in adult zebrafish.