In agriculture, wastewater is used as an alternative source to meet the water and nutritional requirements of plants. However, long-term application of wastewater may degrade soil attributes. This study aimed to evaluate the soil physical quality of Oxisol fertigated with treated sewage effluent (TSE). The experiment was conducted in an area under TSE application for 4 years in Oxisol (625 g kg⁻¹ clay) cultivated with Urochloa brizantha. The treatments consisted of six levels of TSE in irrigation depth, 0%, 11%, 31%, 60%, 87%, and 100%, with four repetitions. Undisturbed and disturbed soils samples were collected in three layers (0.00–0.10 m; 0.10–0.20 m, and 0.20–0.30 m). Aggregation, porosity and water infiltration attributes were evaluated. This work concludes a long-term study on the effects of TSE application on soil properties and on the Urochloa brizantha crop. In other works, carried out in the experimental area of the present study, it was found that TSE fertigation increases the yield and quality of Urochloa brizantha, increases soil fertility and does not lead to soil heavy metal contamination. We note the TSE fertigation does not change the aggregation, porosity, water infiltration rate and organic carbon content in the soil. Irrigation with TSE is recommended in areas with clayey soil and those cultivated with perennial grasses as it does not cause any damage to the physical quality of the soil. Thus, the TSE fertigation can be used on many crops as a source of water and nutrients, reducing the environmental contamination potential.

The extensive use of neonicotinoids (NEOs) has caused the release of wide-ranging of residues to the environment and food, and their potential health risks are now receiving more attention. In this study, three surveys were conducted to obtain the overall profiles of NEO residue levels (seven NEOs and one metabolite) in Chinese tea over a period of seven years. A total of 726 tea samples were tested, and nearly 87% of the samples were found to have detectable NEO residues. The overall average detection frequency of acetamiprid was the highest, reaching 73%. Imidacloprid residues in 4.6% of the samples exceeded the Chinese maximum residue limits, whereas clothianidin and nitenpyram had been detected in Chinese tea samples since 2014. The applications of thiacloprid and thiamethoxam gradually increased, and some tea samples with high residue levels appeared in China. These findings signal the replacement of new and old varieties of NEOs in China. Both long- and short-term cumulative exposures to NEOs were calculated based on optimistic and pessimistic models recommended in the EFSA guidelines. In the three survey periods, the average total imidacloprid-equivalent concentrations were 484.63, 1713.36, and 1148.34 μg/kg, respectively. Combined with the refined point estimates and probabilistic models used in this study, the hazard quotients of NEO residues in tea for Chinese tea consumers were found to be low and within the bounds of safety.

The effect of chronic exposure of freshwater cladoceran Daphnia magna to low, environmentally relevant concentrations i.e 4 μgL⁻¹of ibuprofen (a nonsteroidal anti-inflammatory drug) in a laboratory experiment was studied. We observed the key life history traits of first and fifth generation individuals: age and size at first reproduction, number of first clutch eggs and individual growth rate. Moreover, chosen molecular/subcellular markers of experimental animals stress response such as triglyceride content, heat shock proteins (HSP) expression and DNA:RNA ratio were collected. Overall, chronic exposure to ibuprofen had no significant effect on the molecular markers nor on the life history parameters of the Daphnia. It did, however, caused lethal morphological deformities in embryos and juvenile daphnids. Depending on the clonal affiliation, exposure to a low dosage of ibuprofen over five generations resulted in the deformation of ∼3%–∼10% of the first clutch of offspring. Also, up to 90% of females carried at least one deformed embryo. This is the first time that research has revealed such an effect of ibuprofen on D. magna.

In this study, 45 indoor dust samples and four particulate samples from air-conditioner filters were collected from four different indoor environments in Guangzhou, China, and the concentration and composition of organophosphate tri-esters (OPEs) and organophosphate di-esters (Di-OPs) were determined. Eight of the 10 target OPEs were detected in indoor dust at different detection frequencies (DFs), with tris(2-chloroisopropyl) phosphate and tris(2-chloroethyl) phosphate being the main components. Seven target Di-OPs were detected at different DFs, with diphenyl phosphate being the dominant compound. The total OPEs (∑8 OPEs) and total Di-OPs (∑7 Di-OPs) concentrations varied from 726 to 39,312 ng/g and 68.8–14,766 ng/g, respectively. The ∑8 OPEs concentrations in instrumental houses were significantly higher (p < 0.001) than in three other indoor environments. The varying strengths of the correlation between Di-OPs and their respective parent OPEs was suggestive of their emission sources (e.g., direct application, impurities in OPE formulas, and OPE degradation). The hazard index (HI) values of individual OPEs in residential house were lower than 1, the results suggested a limited human health risk from individual OPEs. However, the total HI value (∑HIs) of OPEs was approximately 1 based on a high exposure scenario and suggested a low risk for toddlers.

Amendment with sewage sludge or biosolids can increase soil fertility but may also transfer biosolid-borne pollutants to the soil and the possible effects on the soil ecosystem are poorly understood, especially long-term effects. A long-term experiment was therefore established to assess the effects of repeated applications of different types of biosolids (fresh domestic, dried domestic and fresh industrial sludges) in field conditions. Nine years of sludge application led to changes in soil chemical and biological properties and generally contributed little to soil nutrient status. However, soil concentrations of potentially toxic elements (PTEs) were elevated by amendment, especially with industrial biosolids. Soil fauna are usually used to decipher the underlying effects of biosolid applications on the soil ecosystem. Here, collembolans (50.9%), nematodes (41.6%) and enchytraeid worms (7.50%) were collected and differentiated into different ecological and trophic groups and their body lengths and PTE concentrations in the body tissues were investigated. The animals showed different responses to the biosolids at population and individual levels. There were substantial changes in epigeic collembolan communities and bacterivorous nematodes increased significantly after biosolid amendment. Biosolid-borne PTEs were major factors and Redundancy (RDA) analysis indicates that collembolan communities were strongly influenced by zinc (Zn). The three groups of soil animals showed similar trends in accumulation of PTEs in the sequence cadmium (Cd) > Zn > copper (Cu), and the bioaccumulation factor (BAF) values of the PTEs were significantly higher in the industrial sludge treatment than in other two treatments with a similar trend of decreasing body length of nematodes. The results indicate that it is potentially risky to use industrial biosolids in the long term, and different species and ecological groups of collembolans and different trophic groups of nematodes should be examined when assessing soil health.

Estuarine ecosystems are important in terms of biodiversity processes because there are intense interactions between the river and sea environments. Phytoplankton and zooplankton have been shown to be ecological indicators of the water quality status in estuary ecosystems. Therefore, a comprehensive evaluation of the effects that multiple pressures have on the phytoplankton and zooplankton communities in estuarine ecosystems is essential. In this study, water samples from 29 stations were collected from the Liaohe Estuary over three different seasons, and biotic factors (i.e., phytoplankton and zooplankton) were obtained and compared. The results showed that there were significant temporal and spatial variations in the phytoplankton and zooplankton communities from the Liaohe Estuary. The correlation analyses showed that water temperature was the most important factor regulating the variation in phytoplankton communities, whereas the main driving force for the zooplankton was nutrient concentrations. Large amounts of nutrients entered the estuary in spring and summer due to intensive human activities in the Liaohe River basin. The inflows by the Liaohe River introduced some phytoplankton and zooplankton into the estuary, such as Coscinodicus asteromphalus, Chaetoceros decipiens, and Schmacheria poplesia. The impacts of Liaohe inflows on the estuary region gradually decreased as the distance from the inlet increased and this change was mediated by marine diffusion. The results from this study will improve knowledge about planktonic communities in estuarine ecosystems and provide a theoretical foundation for estuary environmental management.

Existing studies have typically investigated only the association between single pollutants and health outcomes. However, in the real world, people are exposed to multiple air pollutants simultaneously. The effect of air pollutants on emergency department (ED) visits has not been previously studied in the Sichuan Basin, which is one of the most polluted areas. We collected nonaccidental, respiratory and cardiovascular daily ED visits and daily concentrations of PM₂.₅, PMc, CO, SO₂, NO₂ and O₃ in Chengdu, China, from 2014 to 2016. A weighted variable for the combination of multiple air pollutants was constructed to assess the joint adverse health effects. Each air pollutant was assigned a health-related weight, which indicated the pollutant’s relative contribution to the joint effect. The effects on specific subpopulations (males and females; 15–65 years old and >65 years old) were also examined. With an increase of 10 μg/m³ of the combined multiple air pollutants, the daily ED visits for nonaccidental, respiratory and cardiovascular causes increased by 0.96% (95% CI: 0.51%–1.39%), 1.19% (95% CI: 0.53%, 1.85%) and 4.36% (95% CI: 1.06%, 7.76%) at lag 1, respectively. Males presented more pronounced effects, except for cardiovascular disease, than females. Elderly individuals were found to be more sensitive than young individuals. For nonaccidental and respiratory diseases, the contributions of particulate matter (PM) were dominant among the air pollutants, whereas cardiovascular disease was mainly affected by gaseous air pollutants. The combination of multiple air pollutants was significantly associated with ED visits in the Sichuan Basin, China. The joint effect of the combination of multiple air pollutants was highest for cardiovascular disease at lag 1. The relative contributions of individual pollutants varied by disease and subpopulation. These findings suggest that under different pollution scenarios, preventive strategies should target those with different diseases and different subpopulations.

Copper oxide nanoparticles (CuO NP) have been produced on a large scale due to their economically interesting thermophysical properties. This heightens the concern about risks they may pose on their release into the environment, possibly affecting non-target organisms. Microalga are important organisms in ecotoxicological studies as they are at the base of the aquatic food chain, but information about their biochemical and photosynthetic changes in response CuO NP are still scarce. We studied the effects of CuO NP in Raphidocelis subcapitata using morphological, photosynthetic and biochemical biomarkers. Our results showed that the NP affected microalgal population growth with 0.70 mg Cu L⁻¹ IC₅₀–₉₆ ₕ (inhibition concentration). Based on predicted environmental concentrations of Cu NPs in aquatic environments, our results indicate potential risks of the NP to microalgae. Algal cell size, granularity and photosynthetic efficiencies were affected by the CuO NP at 0.97 and 11.74 mg Cu L⁻¹. Furthermore, lipid metabolism was affected mostly at the highest NP concentration, but at environmentally relevant values (0.012 and 0.065 mg Cu L⁻¹) the production of sterols (structural lipids) and triacylglycerols (reserve lipid) increased. Moreover, we found evidence of cell membrane impairment at the highest CuO NP concentration, and, as a photosynthetic response, the oxygen evolving complex was its main site of action. To the best of our knowledge, this is the first study to date to investigate microalgal lipid composition during CuO NP exposure, showing that it is a sensitive diagnostic tool. This research demonstrated that CuO NP may affect the physiology of R. subcapitata, and because they were observed in a primary producer, we foresee consequences to higher trophic levels in aquatic communities.

Oxidative stress is considered a main commonly reported mechanism of nanoparticles toxicity, so this study aimed to evaluate oxidative stress and biochemical alterations in the haemolymph and digestive gland of snail, Monacha cartusiana exposed to sublethal concentrations of zinc oxide nanoparticles (ZnONPs) for 14 days (d). The results indicated that, ZnONPs induced significant increases in lipid peroxidation (LPO) and lactate dehydrogenase (LDH) in treated animals and did not return to normal levels after recover period. A significant decline of glutathione peroxidase (GPx), glutathione-S-transferase (GST) activities, and glutathione (GSH) content in the haemolymph and digestive gland of snails was observed when compared with control. A significant increase was observed in catalase (CAT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities of treated animals. In general, nano-materials are able to induce oxidative stress in exposed animals. The present findings indicate that, alterations of antioxidant enzyme activities, increase of LPO, LDH, and reducing of GSH content and GST, GPx activities are recognized to oxidative stress and cell damage. This species could be considered a good bioindicator to assess nano-materials exposure.

Data (N = 10644) for US adults aged ≥20 years for 2003–2016 from National Health and Nutrition Examination Survey were analyzed to evaluate the impact of co-occurrence of obesity with diabetes, anemia, albuminuria, and hypertension on concentrations of five perfluoroalkyl acids (PFAA), namely, perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), perfluorodecanoic acid (PFDA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA). For the total population, males, and females, co-occurrence of obesity with hypertension, albuminuria, anemia, and diabetes was found to be associated with lower adjusted geometric means (AGM) than nonobese for every PFAA. For example for females, for PFOS, AGMs for obese with no diseases, hypertension, albuminuria, anemia, and diabetes were 8.2, 10.8, 5.8, 4.6, and 7.7 ng/mL respectively. In comparison, for PFOS, for nonobese females, AGMs for those with no diseases, hypertension, albuminuria, anemia, and diabetes were found to be 8.9, 13.4, 7.7, 6.0, and 10.2 ng/mL respectively. This implies obesity is associated with higher excretion rates. Females, in general, had lower AGMs than males for both obese and nonobese for every PFAA for every disease group. For example, percent ratios of obese females to males AGMs for PFOA were 66.7%, 87.1%, 88.2%, 70.6%, and 90% for those with no diseases, hypertension, albuminuria, anemia, and diabetes respectively. The ratios of obese to nonobese AGMs for females were lower than males for every PFAA for those with no diseases and hypertension only. For example, for PFOA for those with no diseases, obese to nonobese AGM ratios were 87% for females and 100% for males. Thus, additional excretion of certain PFAAs due to obesity is higher in females than males for those with no diseases and hypertension only.