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To investigate the effects of regulation on environmental pollution under Chinese-style fiscal decentralization, this research analyzes annual data over the period 2003 to 2017 covering 30 provinces in China with the spatial economic model. The empirical results show significant spatial agglomeration effects on the emissions of wastewater, sulfur dioxide, and solid waste. Environmental regulation helps reduce discharge of wastewater and solid waste, but does not help reduce the emission of sulfur dioxide; because there is significantly positive externality in treating pollutants with high fluidity, cost is larger than revenue for local governments. The relationship between fiscal decentralization and pollutants shapes an inverted U-shaped curve. We finally offer some implications in accordance with our empirical finding, such as the intensity of environmental regulation should be suitable for economic development, different measures should be taken based on the fluidity of pollutants, and a new evaluation system should be established.

This study investigated for the first time the effects of ZnO nanoparticle (NP) chronic exposure (28 days) on Tigriopus fulvus. Acute toxicity (48 h) of three Zn chemical forms was assessed as well including the following: (a) ZnO nanoparticles (NPs), (b) Zn²⁺ from ZnO NP suspension after centrifugation (supernatant) and (c) ZnSO₄ H₂O. Physical-chemical and electronic microscopies were used to characterize spiked exposure media. Results showed that the dissolution of ZnO NPs was significant, with a complete dissolution at lowest test concentrations, but nano- and micro-aggregates were always present. Acute test evidenced a significant higher toxicity of Zn²⁺ and ZnSO₄ compared to ZnO NPs. The chronic exposure to ZnO NPs caused negative effects on the reproductive traits, i.e. brood duration, brood size and brood number at much lower concentrations (≥ 100 μg/L). The appearance of ovigerous females was delayed at higher concentrations of ZnO NPs, while the time required for offspring release and the percentage of non-viable eggs per female were significantly increased. ZnO NP subchronic exposure evidenced its ability to reduce T. fulvus individual reproductive fitness, suggesting that ZnO NPs use and release must be carefully monitored. Graphical abstract Graphical Abstract.

Solar energy systems are a cheaper and easy solution to cope with severe energy crisis especially in emerging economies including Turkey which exerted huge efforts to enhance the existing solar power projects. However, the selection of the optimal site for the installation of solar projects needs vigorous investigation through various factors. Adequate quantitative scientific research is required for the process of site selection in Turkey. This paper categorizes various sites in Turkey through various factors such as economic, environmental, and social factors. Various major criteria have been combined through mathematical development to install the solar power project in remote areas of Turkey. The scientific evaluation of remote and rural solar projects in Turkey has been taken as a case study in the current paper. Additionally, the analytical hierarchy process (AHP) and F-VIKOR methods were used to aggregate the criteria. The results show that economic and social ratio is significant, whereas the transmission matrix, land cost, and the sun irradiance got a major score in order to generate electricity. The study results show that total sunshine time per year determined is 2741 h (a total of 7.5 h per day) and the total solar energy obtained each year is 1527 kWh per square meter per year (a total of 4.18 kWh per square meter per day).

Chromium is considered an environmental pollutant of much concern whose toxicity depends, to a great extent, on its valence state, with Cr(VI) being more soluble, bioavailable, and toxic, compared to Cr(III). Nanoremediation is a promising strategy for the remediation of metal pollutants by changing their valence state. However, among other aspects, its effectiveness for soil remediation is seriously hampered by the interaction of nanoparticles with soil organic matter. In this study, soil was (i) amended with two doses of a municipal solid organic waste and (ii) artificially polluted with 300 mg Cr(VI) kg⁻¹ DW soil. After a period of aging, a nanoremediation treatment with nanoscale zero-valent iron particles (1 g nZVI kg⁻¹ DW soil) was applied. The efficiency of the remediation treatment was assessed in terms of Cr(VI) immobilization and recovery of soil health. The presence of the organic amendment caused (i) a decrease of redox potential, (ii) Cr(VI) immobilization via its reduction to Cr(III), (iii) a stimulation of soil microbial communities, and (iv) an improvement of soil health, compared to unamended soil. By contrast, nZVI did not have any impact on Cr(VI) immobilization nor on soil health. It was concluded that, unlike the presence of the organic amendment, nanoremediation with nZVI was not a valid option for soils polluted with Cr(VI) under our experimental conditions.

CHEMTAX is a mathematical software for phytoplankton composition evaluation using pigment composition. Although this method has been previously applied in the ocean environment, we firstly utilized the combination of matrix factorization program CHEMTAX and high-performance liquid chromatography (HPLC) to characterize the phytoplankton community from a river system (western part of Weihe River Basin). The obtained results were compared with those from microscopic examination. Based on the comparison, it is suggested that after increasing the ratio of characteristic pigment to chlorophyll a of diatoms and euglena, the diatoms calculated by the CHEMTAX method accounted for 80% of the total biomass, and the results were consistent with microscopic examination, but diatoms obtained from F2, C1 and W5 sample sites were significantly overestimated 33%~60%. The comparison also showed that the model always underestimated cyanobacteria (sample sites F2, C1 were underestimated 25%) and euglena were overestimated (sample sites W3, Q1 were respectively overestimated 33%, 23%), but for chlorophytes, both overestimation and underestimation could occur. When the relevant results from previous applications in the ocean phytoplankton community evaluation were taken into consideration, it can be concluded that CHEMTAX-HPLC method was not accurate enough to characterize the phytoplankton communities in the freshwater (river/lake) ecosystem.

The carbon sequestration capacity of the forest ecosystem normally increases overage due to the carbon dynamic in below canopy and soil. The carbon dynamic is reflective of the forest characteristics and their interactions with climate, topographic, and soil conditions. In this study, we measured the carbon content and carbon density of canopy, shrub, understory vegetation, litter, and soil, and assessed carbon dynamics in three forest ecosystems (Cunninghamia lanceolate, Pinus massoniana, and Evergreen broad-leaved forests) with a combination of data from Fujian Provincial forest resource inventory. This study showed that the carbon content of the canopy layers increased over time, and the carbon content of the topsoil (0–30 cm) in the young forests was significantly higher than that in other age groups in Cunninghamia lanceolata forest and Pinus massoniana forest. Due to the carbon differences in the soil layer, the carbon stocks of the C. lanceolata forest and the P. massoniana forest declined from 1996 to 2007, but the carbon stocks of Evergreen broad-leaved forest increased. Besides, using the traditional carbon content coefficient (0.5) might underestimate the carbon sequestration potential of these forest ecosystems, especially for the mature forests. The coniferous forests displayed a short-term reduction in the carbon stocks of ecosystems between 10 and 20 years after afforestation, and the decline cannot be ignored in the carbon budget.

Emamectin benzoate (EMB), which is used as a pesticide in agriculture, household, and veterinary medicine, can cause tissue damage with oxidative toxicity and can be considered as inducing apoptosis. In the present study, male mice were conducted by oral administration in EMB doses 25, 50, and 100 (mg/kg/day) for 14 days. Glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) levels using spectrophotometric methods were measured. 8-hydroxy-2′-deoxyguanosine (8-OHdG) which is DNA oxidation biomarker and, stress protein (HSP70) levels, caspase 3 enzyme activities were measured by ELISA techniques. This study shows that in vivo administration of EMB caused a marked induction of oxidative damage in liver tissue as demonstrated by an increased level of TBARS and reduced GSH level. The increase in HSP70 level did not prevent the apoptosis caused by the increase of caspase 3 enzyme activity. Toxicity caused by EMB also showed the formation of genotoxicity with an increase in DNA oxidation biomarker 8-OHdG levels. As a result of the study, the effects of toxicity caused by EMB on lipid; protein; and DNA, structural macromolecules in cells, and the importance of enzymatic and non-enzymatic bonds of the cell’s protective systems were determined. Consequently, under experimental conditions, EMB exposure caused toxicity in the liver of male mice, and significant adverse effects were determined with biomarkers.

Leptolyngbya boryana (L. boryana) is a typical filamentous cyanobacterium that is widely distributed in aquatic ecosystems and is considered to play an important role in the arsenic biogeochemical cycle. Our results showed that L. boryana resisted arsenite (As(III)) and arsenate (As(V)) concentrations up to 0.25 mM and 5 mM, respectively. When exposed to 100 μM As(III) or As(V) for 4 weeks, L. boryana accumulated as much arsenic as 570.0 mg kg⁻¹ and 268.5 mg kg⁻¹, respectively. After the 4-week exposure to As(III) and As(V), organoarsenicals including dimethylarsenate (DMAs(V)) and oxo-arsenosugar-phosphate (Oxo-PO₄) were detected in the cells of L. boryana, while inorganic arsenic, especially As(V), was still the main species in both the cells and medium. Furthermore, arsenic oxidation was observed to be solely caused by L. boryana cells and was considered the dominant detoxification pathway. In conclusion, due to its powerful arsenic accumulation, biotransformation, and detoxification abilities, L. boryana might play an important role in arsenic remediation in aquatic environments.

In the present work, polyphenol oxidase (PPO) enzyme was purified from potato peel using three-phase partitioning (TPP). In this method, ammonium sulfate and t-butanol were added to precipitate the protein/enzyme from the crude aqueous extract. The PPO enzyme precipitated as an interfacial layer between the upper organic solvent phase and lower aqueous phase. Different purification parameters such as crude extract to t-butanol ratio, ammonium sulfate concentration, temperature, and pH were optimized for TPP. About 69% PPO enzyme activity was recovered in a single step of TPP with 9.2-fold purification. The sodium dodecyl sulfate–polyacrylamide gel electrophoresis profile of partially purified PPO enzyme showed molecular weight in the range of about 30–40 kDa. The PPO enzyme was then investigated for the fabrication of a portable, cost-effective, and disposable colorimetric paper biosensor or colorimetric “test strips” for detection of phenolic contaminants. PPO and a chromophore reagent (3-methyl-2-benzothiazolinone hydrazine) generated a range of color in the presence of phenolic compounds (catechol, phenol, p-cresol, 4-methyl catechol) within 15 min, and limit of detection was found to be 0.5 μM. The biosensor worked in a broad range of pH from 3 to 11 and showed good storage stability at 25 °C and 4 °C for 30 days with no significant loss of activity. The biosensor was also applied on environmental water and urine sample to show reliability of biosensor.