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Molecular mechanisms of developmental toxicities of azoxystrobin and pyraclostrobin toward zebrafish (Danio rerio) embryos: Visualization of abnormal development using two transgenic lines
2021
Kim, Chaeeun | Choe, Hyeseung | Park, Jungeun | Kim, Gayoung | Kim, Kyeongnam | Jeon, Hwang-Ju | Moon, Joon-Kwan | Kim, Myoung-Jin | Lee, Sung-Eun
Azoxystrobin (AZ) and pyraclostrobin (PY) are strobilurin fungicides that inhibit fungal mitochondrial respiration. In this study, a representative model, zebrafish (Danio rerio), was used as a test species for acute and developmental toxicity. Survival and malformation rates were observed only PY-treated embryos, with an LC₅₀ value of 77.75 ppb accompanied by a dramatic decrease in hatching rate, while AZ did not show great mortality. Morphological changes were observed in PY-treated embryos with the occurrence of pericadial edema at 25 ppb. A delay in growth was observed after treatment with pyraclostrobin at 50 ppb. Use of genetically engineered Tg(cmlc:EGFP) allowed fluorescence observation during heart development. PY interfered with normal heart development via upregulation of the nppa gene responsible for the expression of natriuretic peptides. Heart function was dramatically reduced as indicated by reduced heart rates. Increased expression of the nppa gene was also seen in AZ-treated embryos. The expression level of cyp24a1 was also up-regulated, while ugt1a1 and sult1st6 were down-regulated after treatment of zebrafish embryos with AZ or PY. Overall, strobilurin fungicides might inhibit normal heart formation and function within the range of concentrations tested.
显示更多 [+] 显示较少 [-]Comparative study on the characteristics of condensable particulate matter emitted from three kinds of coal
2021
Feng, Yupeng | Li, Yuzhong | Zhang, Xiaoyu | Su, Shiqian | Zhang, Zhuping | Gan, Zongwei | Dong, Yong
Condensable particulate matter (CPM) is quickly formed by several gaseous substances in flue gas after emission and belongs to primary particulate matter emitted into the atmosphere by stationary sources. Many studies have shown that current CPM emissions from coal-fired stationary sources far exceed filterable particulate matter, and the issue of CPM emissions has attracted widespread attention. The current research on CPM mainly focuses on its emission characteristics in stationary sources and its migration characteristics in pollutant-controlled equipment, lacking the characteristics of CPM directly generated by fuel combustion. In this study, a one-dimensional flame furnace is used as a stable source of flue gas in the laboratory. The concentration (including inorganic and organic components) and chemical composition (including water-soluble ions, metal elements, and organic matters) of CPM are obtained by the combustion of three kinds of coal (Inner Mongolia lignite, Jinjie bitumite, and Ningxia anthracite) that China consumes in large amounts. The characteristics of CPM including emission factors obtained from different kinds of coal under various experimental conditions are comparatively analyzed. Moreover, a scanning electron microscope–energy-dispersive spectrometer is used to observe the morphology and elemental composition of CPM collected on the filter membrane after the combustion of different kinds of coal. Results show that CPM is mainly in the form of droplets or spheres, and heavy metal elements such as Hg, As, Se, and Sb are detected. These valuable data will enrich people’s understanding of the characteristics of CPM generated by coal combustion and can provide data references for evaluating the influence of CPM on the environment.
显示更多 [+] 显示较少 [-]Evaluation and comparison of the mitochondrial and developmental toxicity of three strobilurins in zebrafish embryo/larvae
2021
Yang, Lihua | Huang, Tao | Li, Ruiwen | Souders, Christopher L. | Rheingold, Spencer | Tischuk, Claire | Li, Na | Zhou, Bingsheng | Martyniuk, Christopher J.
Strobilurin fungicides have been frequently detected in aquatic environments and can induce mitochondrial toxicity to non-target aquatic organisms. However, the derived toxicity and subsequent mechanisms related to their adverse effects are not fully elucidated. In the present study, we compared the mitochondrial and developmental toxicity of azoxystrobin, pyraclostrobin, and trifloxystrobin using zebrafish embryo/larvae. The results showed that all three strobilurins inhibited mitochondrial and non-mitochondrial respiration (the potency is pyraclostrobin ≈ trifloxystrobin > azoxystrobin). Behavioral changes indicated that sublethal doses of pyraclostrobin and azoxystrobin caused hyperactivity of zebrafish larvae in dark cycles, whereas trifloxystrobin resulted in hypoactivity of zebrafish larvae. In addition, pyraclostrobin exposure impaired the inflation of swim bladder, and caused down-regulation of annexin A5 (anxa5) mRNA levels, and up-regulated transcript levels of pre-B-cell leukemia homeobox 1a (pbx1a); conversely, azoxystrobin and trifloxystrobin did not cause detectable effects with swim bladder inflation. Molecular docking results indicated that azoxystrobin had higher interacting potency with iodotyrosine deiodinase (IYD), prolactin receptor (PRLR), antagonistic conformation of thyroid hormone receptor β (TRβ) and glucocorticoid receptor (GR) compared to pyraclostrobin and trifloxystrobin; pyraclostrobin and azoxystrobin were more likely to interact with the antagonistic conformation of TRβ and GR, respectively. These results may partially explain the different effects observed in behavior and swim bladder inflation, and also point to potential endocrine disruption induced by these strobilurins. Taken together, our study revealed that all three strobilurins alter mitochondrial bioenergetics and cause developmental toxicity. However, the toxic phenotypes and underlying mechanisms of each chemical may differ, and this requires further investigation. Pyraclostrobin showed higher mitochondrial toxicity at lethal doses and higher developmental toxicity at sublethal doses compared to the two other strobilurins tested. These results provide novel information for toxicological study as well as risk assessment of strobilurin fungicides.
显示更多 [+] 显示较少 [-]Study of a hydrodynamic threshold system for controlling dinoflagellate blooms in reservoirs
2021
Song, Yang | Shen, Lian | Zhang, Linglei | Li, Jia | Chen, Min
Hydrodynamic conditions often affect the eutrophication process and play a key role in algal growth in reservoirs. A promising approach for controlling algal blooms in reservoirs is to create adverse hydrodynamic conditions by implementing reservoir operation strategies. However, research on this method is still nascent and does not support practical applications due to the lack of quantitative hydrodynamic thresholds. In this paper, field observations of algal growth from April 2015 to August 2016 were conducted, and a three-dimensional (3D) model that couples hydrodynamics and water temperatures for the Zipingpu Reservoir was established. Low flow velocities (V) and low Reynolds numbers (Re) in the Longchi tributary are favorable for dinoflagellate growth and accumulation, which can explain why dinoflagellate blooms are more likely to occur in the tributary. A temperature of 18–22 °C is considered a precondition for Peridiniopsis penardii blooms, suggesting that freshwater dinoflagellate species may prefer lower temperatures than marine dinoflagellate species. Shallow mixing layer depth (Zₘᵢₓ) is conducive to Peridiniopsis penardii gathering in the upper water layers and promotes growth. The shallow euphotic layer depth (Zₑᵤ) was speculated to promote the dominance of this species by stimulating its heterotrophy and inhibiting other algal autotrophy. Furthermore, a boundary line analysis was introduced to characterize the relationships between algal biomass and hydrodynamic indicators. Thus, the thresholds for V, Re, and Zₘᵢₓ/Zₑᵤ were determined to be 0.034 m s⁻¹, 6.7 × 10⁴, and 1.7, respectively. Either accelerating horizontal flow to exceed the thresholds of V and Re or facilitating vertical mixing to exceed the threshold of Zₘᵢₓ/Zₑᵤ can prevent dinoflagellate blooms. Therefore, the summarized hydrodynamic threshold system is suggested to be an effective standard for controlling dinoflagellate blooms in the reservoir. Moreover, this study can provide a useful reference for understanding the mechanism of freshwater dinoflagellate blooms.
显示更多 [+] 显示较少 [-]Valorisation of medical waste through pyrolysis for a cleaner environment: Progress and challenges
2021
Su, Guangcan | Ong, Hwai Chyuan | Ibrahim, Shaliza | Fattah, I. M Rizwanul | Mofijur, M. | Chong, Cheng Tung
The COVID-19 pandemic has exerted great shocks and challenges to the environment, society and economy. Simultaneously, an intractable issue appeared: a considerable number of hazardous medical wastes have been generated from the hospitals, clinics, and other health care facilities, constituting a serious threat to public health and environmental sustainability without proper management. Traditional disposal methods like incineration, landfill and autoclaving are unable to reduce environmental burden due to the issues such as toxic gas release, large land occupation, and unsustainability. While the application of clean and safe pyrolysis technology on the medical wastes treatment to produce high-grade bioproducts has the potential to alleviate the situation. Besides, medical wastes are excellent and ideal raw materials, which possess high hydrogen, carbon content and heating value. Consequently, pyrolysis of medical wastes can deal with wastes and generate valuable products like bio-oil and biochar. Consequently, this paper presents a critical and comprehensive review of the pyrolysis of medical wastes. It demonstrates the feasibility of pyrolysis, which mainly includes pyrolysis characteristics, product properties, related problems, the prospects and future challenges of pyrolysis of medical wastes.
显示更多 [+] 显示较少 [-]Serum concentrations of organochlorine pesticides, biomarkers of oxidative stress, and risk of breast cancer
2021
Miao, Yu | Rong, Mao | Li, Min | He, Heng | Zhang, Li | Zhang, Shanshan | Liu, Chong | Zhu, Ying | Deng, Yan-Ling | Chen, Pan-Pan | Zeng, Jia-Yue | Zhong, Rong | Mei, Su-Rong | Miao, Xiao-Ping | Zeng, Qiang
Studies have documented that exposure to organochlorine pesticides (OCPs) is linked with breast cancer, but the underlying biological mechanisms are still unknown. This study included 313 women diagnosed with breast cancer and 313 controls in Wuhan, China, and measured 18 OCPs in serum and 3 oxidative stress biomarkers in urine. Multivariable adjusted regression models were used to evaluate the associations among OCPs, oxidative stress biomarkers, and breast cancer. The mediating effect of oxidative stress was assessed by mediation analysis. We observed that most OCPs were positively associated with risk of breast cancer (all FDR-P values < 0.05 or 0.10). Moreover, we found that p,p'-DDT, p,p'-DDD, dieldrin, heptachlor, and heptachlor epoxide were positively associated with 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA) and 8-iso-prostaglandin F₂α (8-isoPGF₂α), which in turn were positively associated with risk of breast cancer. Mediation analysis indicated that HNE-MA and 8-isoPGF₂ɑ mediated the positive associations between these OCPs and risk of breast cancer, with mediating proportion ranging from 6.23% to 19.9%. Our results suggest that lipid peroxidation may mediate the positive associations between OCP exposures and risk of breast cancer.
显示更多 [+] 显示较少 [-]Efficient utilization of Iris pseudacorus biomass for nitrogen removal in constructed wetlands: Combining alkali treatment
2021
Gu, Xushun | He, Shengbing | Huang, Jungchen
Aquatic plant biomass like Iris pseudacorus can be used as electron donor to improve denitrification performance in subsurface constructed wetlands. However, the phenomenon that the nitrogen removal rate declined in the terminal stage restricted the utilization of litters. In terms of this problem, this study investigated the performance of the used biomass through alkali treatment on nitrogen removal and analyzed the effect of alkali treatment on the component and structure of biomass and microbial community. The results showed that the alkali-treated biomass could further enhance the nitrogen removal by nearly 15% compared with used ones. The significant damage of cell walls and compact fibers containing cellulose and lignin through alkali treatment mainly resulted in the improvement of carbon release and nitrogen removal. With the addition of alkali-treated biomass, the richness index of microbes was higher compared with other biomass materials. Furthermore, the abundance of denitrification related genera increased and the abundance of genera for nitrification was maintained. Based on these finds, a mode of a more efficient Iris pseudacorus self-consumed subsurface flow constructed wetlands was designed. In this mode, the effluent total nitrogen could be stabilized below 5 mg L⁻¹ for nine months and the weight of litters could be further cut down by 75%. These findings would contribute to efficient utilization of plant biomass for nitrogen removal enhancement and final residue reduction in the wetlands.
显示更多 [+] 显示较少 [-]Electrocatalytic inactivation of antibiotic resistant bacteria and control of antibiotic resistance dissemination risk
2021
Liu, Haiyang | Hua, Xiuyi | Zhang, Ya-nan | Zhang, Tingting | Qu, Jiao | Nolte, Tom M. | Chen, Guangchao | Dong, Deming
Antibiotic resistance in environmental matrices becomes urgently significant for public health and has been considered as an emerging environmental contaminant. In this work, the ampicillin-resistant Escherichia coli (AR E. coli) and corresponding resistance genes (blaTEM₋₁) were effectively eliminated by the electrocatalytic process, and the dissemination risk of antibiotic resistance was also investigated. All the AR E. coli (∼8 log) was inactivated and 8.17 log blaTEM₋₁ was degraded by the carbon nanotubes/agarose/titanium (CNTs/AG/Ti) electrode within 30 min. AR E. coli was inactivated mainly attributing to the damage of cell membrane, which was attacked by reactive oxygen species and subsequent leakage of intracellular cytoplasm. The blaTEM₋₁ was degraded owing to the strand breaking in the process of electrocatalytic degradation. Furthermore, the dissemination risk of antibiotic resistance was effectively controlled after being electrocatalytic treatment. This study provided an effective electrocatalytic technology for the inactivation of antibiotic resistant bacteria and control of antibiotic resistance dissemination risk in the aqueous environment.
显示更多 [+] 显示较少 [-]Two novelty learning models developed based on deep cascade forest to address the environmental imbalanced issues: A case study of drinking water quality prediction
2021
Chen, Xingguo | Liu, Houtao | Liu, Fengrui | Huang, Tian | Shen, Ruqin | Deng, Yongfeng | Chen, Da
Environmental quality data sets are typically imbalanced, because environmental pollution events are rarely observed in daily life. Prediction of imbalanced data sets is a major challenge in machine learning. Our recent work has shown deep cascade forest (DCF), as a base learning model, is promising to be recommended for environmental quality prediction. Although some traditional models were improved by introducing the cost matrix, little is known about whether cost matrix could enhance the prediction performance of DCF. Additionally, feature extraction is also an important way to potentially improve the model's ability to predict the imbalanced data. Here, we developed two novelty learning models based on DCF: cost-sensitive DCF (CS-DCF) and DCF that combines unsupervised learning models and greedy methods (USM-DCF-G). Subsequently, CS-DCF and USM-DCF-G were successfully verified by an imbalanced drinking water quality data set. Our data presented both CS-DCF and USM-DCF-G show better prediction performance than that of DCF alone did. In particular, USM-DCF-G shows the best performance with the highest F1-score (95.12 ± 2.56%), after feature extraction and selection by using unsupervised learning models and greedy methods. Thus, the two learning models, especially USM-DCF-G, were promising learning models to address environmental imbalanced issues and accurately predict environmental quality.
显示更多 [+] 显示较少 [-]Effects of microplastics on the functional traits of aquatic benthic organisms: A global-scale meta-analysis
2021
Berlino, M. | Mangano, M.C. | De Vittor, C. | Sarà, G.
Microplastics are widespread in the aquatic environment and thus available for many organisms at different trophic levels. Many scientific papers focus their attention on the study of the effects of microplastics on different species at individual level. Here we performed a global scale meta-analysis focusing our work on the study of the effect of microplastics on the functional traits of aquatic benthic organisms. Overall, microplastics showed a moderate negative effect on the examined functional traits of benthic organisms. Our results show that some crucial functional traits, such as those linked to behaviour and feeding, appear to be unaffected by microplastics. In contrast, traits related to the capacity of organisms to assimilate energy are affected. Moreover, traits with possible effects at population level appear to be negatively affected by microplastics. We discuss how the direct impact of organismal performance may have indirect repercussions at higher levels in the ecological hierarchy and represent a risk for the stability and functioning of the ecosystem.
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