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Bioconcentration of perfluoroalkyl substances by Chironomus plumosus larvae in water with different types of dissolved organic matters
2016
Wen, Wu | Xia, Xinghui | Chen, Xi | Wang, Haotian | Zhu, Baotong | Li, Husheng | Li, Yang
The effects of four types of dissolved organic matters (DOM) on the bioconcentration of perfluoroalkyl substances (PFASs) in Chironomus plumosus larvae have been studied. The PFASs included perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnA), and perfluorododecanoic acid (PFDoA). The DOM included humic acid (HA), fulvic acid (FA), tannic acid (TA), and a protein, peptone (PEP), and their concentrations ranged from 0 to 50 mg L−1. The results showed that, upon bioconcentration equilibrium, the body burdens of longer perfluoroalkyl chain PFASs (PFOS, PFDA, PFUnA and PFDoA) decreased with PEP and HA concentrations while increased with FA and TA concentrations. When FA and TA concentrations increased from 0 to 50 mg L−1, body burdens of these PFASs increased by 7.5%–148.8% and 5.7%–37.1%, respectively. However, the DOM had no significant impact on the body burdens of shorter perfluoroalkyl chain PFASs (PFOA and PFNA). All of the four types of DOM lowered not only the uptake rate constants (ku) of PFASs due to the decrease of freely dissolved PFAS concentrations, but also the elimination rate constants (ke) due to the inhibition effect of DOM on the PFAS elimination from the larvae. The reduction in the two constants varied with both DOM and PFAS types. In the presence of PEP and HA with larger molecular weights, the ku values decreased more than ke, leading to the decreased body burdens of longer perfluoroalkyl chain PFASs. As for FA and TA with smaller molecular weights, the ke values decreased more than ku, resulting in increased body burdens of longer perfluoroalkyl chain PFASs. This study suggests that the effects of DOM on PFAS bioconcentration depend not only on the concentration but also on the molecule weight of DOM, which should be considered in the bioavailability assessment of PFASs.
显示更多 [+] 显示较少 [-]Biomonitoring of air pollution using antioxidative enzyme system in two genera of family Pottiaceae (Bryophyta)
2016
Bansal, Pooja | Verma, Sonam | Srivastava, Alka
Bryophyte particularly mosses, have been found to serve as reliable indicators of air pollution and can serve as bryometers–biological instruments for measuring air pollution. They are remarkable colonizers, as they have the ability to survive in adverse environments and are also particular in their requirement of environmental conditions, which makes them appropriate ecological indicators. The purpose of this study was to evaluate the activity of antioxidative enzymes in two mosses viz., Hyophila rosea R.S. Williams and Semibarbula orientalis (Web.) Wijk. & Marg. and assess their suitability as biomonitors. Three different locations viz., Lucknow University, Residency (contaminated sites) and Dilkusha Garden (reference site) within Lucknow city with different levels of air pollutants were used for comparison. Our results indicate that air pollution caused marked enhancement in activity of antioxidative enzymes viz., catalase, peroxidase and superoxide dismutase. All the three are capable of scavenging reactive oxygen species. In the genus S. orientalis, catalase, peroxidase and superoxide dismutase activity was minimum at the reference site Dilkusha Garden and was significantly higher at the two contaminated sites for catalase and peroxidase, whereas the difference was non significant for superoxide dismutase. In H. rosea the activity of catalase and peroxidase at the three locations was almost similar, however superoxide dismutase activity showed a significant increase in the two contaminated sites when compared to the reference site, the value being highest for Lucknow University site. It was thus observed that the two genera, from the same location, showed difference in the activity of the antioxidative enzymes. Based on our results, we recommend bryophytes as good monitors of air pollution.
显示更多 [+] 显示较少 [-]Silver nanoparticles impact the functional role of Gammarus roeseli (Crustacea Amphipoda)
2016
Andreï, Jennifer | Pain-Devin, Sandrine | Felten, Vincent | Devin, Simon | Giambérini, Laure | Mehennaoui, Kahina | Cambier, Sébastien | Gutleb, Arno C. | Guérold, François
Silver nanoparticles (nAg) are widely used in consumer products and the risk associated with their potential release into freshwater ecosystems needs to be addressed using environmentally realistic exposure concentrations. Here, the effects of low concentrations (0.5–5 μg L⁻¹) of two different sized nAg (10 and 60 nm) and a silver nitrate positive control were evaluated in Gammarus roeseli following exposure for 72 h. Cellular, individual and functional endpoints were independently studied and the most striking results were reported for functional endpoints. Indeed, without a change in their feeding activity, the gammarids produced significantly fewer fine particles of organic matter when exposed to nAg, even at 0.5 μg L⁻¹ of 10 nm nAg. These functional endpoints seem to be efficient markers for detecting the early effects of nAg on G. roeseli.
显示更多 [+] 显示较少 [-]Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India
2016
Chettri, Bobby | Mukherjee, Arghya | Langpoklakpam, James S. | Chattopadhyay, Dhrubajyoti | Singh, Arvind K.
Bacterial degradation of crude oil in response to nutrient treatments has been vastly studied. But there is a paucity of information on kinetic parameters of crude oil degradation. Here we report the nutrient stimulated kinetic parameters of crude oil degradation assessed in terms of CO2 production and oil removal by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2. The hydrocarbon degradation rate of P. aeruginosa AKS1 in oil only amended sediment was 10.75 ± 0.65 μg CO2-C g−1 sediment day−1 which was similar to degradation rate in sediments with no oil. In presence of both inorganic N & P, the degradation rate increased to 47.22 ± 1.32 μg CO2-C g−1 sediment day−1. The half-saturation constant (Ks) and maximum degradation rate (Vmax) for P. aeruginosa AKS1 under increasing N and saturating P concentration were 13.57 ± 0.53 μg N g−1 sediment and 39.36 ± 1.42 μg CO2-C g−1 sediment day−1 respectively. The corresponding values at increasing P and a constant N concentration were 1.60 ± 0.13 μg P g−1 sediment and 43.90 ± 1.03 μg CO2-C g−1 sediment day−1 respectively. Similarly the degradation rate of Bacillus sp. AKS2 in sediments amended with both inorganic nutrients N & P was seven fold higher than the rates in oil only or nutrient only treated sediments. The Ks and Vmax estimates of Bacillus sp. AKS2 under increasing N and saturating P concentration were 9.96 ± 1.25 μg N g−1 sediment and 59.96 ± 7.56 μg CO2-C g−1 sediment day−1 respectively. The corresponding values for P at saturating N concentration were 0.46 ± 0.24 μg P g−1 sediment and 63.63 ± 3.54 μg CO2-C g−1 sediment day−1 respectively. The rates of CO2 production by both isolates were further stimulated when oil concentration was increased above 12.5 mg g−1 sediment. However, oil degradation activity declined at oil concentration above 40 mg g−1 sediment when treated with constant nutrient: oil ratio. Both isolates exhibited alkane hydroxylase activity but aromatic degrading catechol 1, 2-dioxygenase and catechol 2, 3-dioxygenase activities were shown by P. aeruginosa AKS1 only.
显示更多 [+] 显示较少 [-]Remediation of antimony-rich mine waters: Assessment of antimony removal and shifts in the microbial community of an onsite field-scale bioreactor
2016
Sun, Weimin | Xiao, Enzong | Kalin, Margarete | Krumins, Valdis | Dong, Yiran | Ning, Zengping | Liu, Tong | Sun, Min | Zhao, Yanlong | Wu, Shiliang | Mao, Jianzhong | Xiao, Tangfu
An on-site field-scale bioreactor for passive treatment of antimony (Sb) contamination was installed downstream of an active Sb mine in Southwest China, and operated for one year (including a six month monitoring period). This bioreactor consisted of five treatment units, including one pre-aerobic cell, two aerobic cells, and two microaerobic cells. With the aerobic cells inoculated with indigenous mine water microflora, the bioreactor removed more than 90% of total soluble Sb and 80% of soluble antimonite (Sb(III)). An increase in pH and decrease of oxidation-reduction potential (Eh) was also observed along the flow direction. High-throughput sequencing of the small subunit ribosomal RNA (SSU rRNA) gene variable (V4) region revealed that taxonomically diverse microbial communities developed in the bioreactor. Metal (loid)-oxidizing bacteria including Ferrovum, Thiomonas, Gallionella, and Leptospirillum, were highly enriched in the bioreactor cells where the highest total Sb and Sb(III) removal occurred. Canonical correspondence analysis (CCA) indicated that a suite of in situ physicochemical parameters including pH and Eh were substantially correlated with the overall microbial communities. Based on an UPGMA (Unweighted Pair Group Method with Arithmetic Mean) tree and PCoA (Principal Coordinates Analysis), the microbial composition of each cell was distinct, indicating these in situ physicochemical parameters had an effect in shaping the indigenous microbial communities. Overall, this study was the first to employ a field-scale bioreactor to treat Sb-rich mine water onsite and, moreover, the findings suggest the feasibility of the bioreactor in removing elevated Sb from mine waters.
显示更多 [+] 显示较少 [-]Investigating the mechanisms of Ni uptake and sub-lethal toxicity in the Atlantic killifish Fundulus heteroclitus in relation to salinity
2016
Blewett, Tamzin A. | Ransberry, Victoria E. | McClelland, Grant B. | Wood, Chris M.
The Atlantic killifish (Fundulus heteroclitus) is a resilient estuarine species that may be subjected to anthropogenic contamination of its natural habitat, by toxicants such as nickel (Ni). We investigated Ni accumulation and potential modes of Ni toxicity, in killifish, as a function of environmental salinity. Killifish were acclimated to 4 different salinities [0 freshwater (FW), 10, 30 and 100% seawater (SW)] and exposed to 5 mg/L of Ni for 96 h. Tissue Ni accumulation, whole body ions, critical swim speed and oxidative stress parameters were examined. SW was protective against Ni accumulation in the gills and kidney. Addition of Mg and Ca to FW protected against gill Ni accumulation, suggesting competition with Ni for uptake. Concentration-dependent Ni accumulation in the gill exhibited saturable relationships in both FW- and SW-acclimated fish. However SW fish displayed a lower Bmax (i.e. lower number of Ni binding sites) and a lower Km (i.e. higher affinity for Ni binding). No effect of Ni exposure was observed on critical swim speed (Ucrit) or maximum rate of oxygen consumption (MO2max). Markers of oxidative stress showed either no effect (e.g. protein carbonyl formation), or variable effects that appeared to depend more on salinity than on Ni exposure. These data indicate that the killifish is very tolerant to Ni toxicity, a characteristic that may facilitate the use of this species as a site-specific biomonitor of contaminated estuaries.
显示更多 [+] 显示较少 [-]Temporal dynamics of microcystins in Limnodrilus hoffmeisteri, a dominant oligochaete of hypereutrophic Lake Taihu, China
2016
Xue, Qingju | Steinman, Alan D. | Su, Xiaomei | Zhao, Yanyan | Xie, Liqiang
We examined the bioaccumulation of three microcystin (MC) congeners (MC-LR, MC-RR and MC-YR) in the oligochaete Limnodrilus hoffmeisteri from July 2013 through June 2014 in Lake Taihu, China. Environmental parameters and MCs in sediment, phytoplankton and water column also were examined. L. hoffmeisteri accumulated extremely high MC concentrations during the warmest months, with a maximum value of 11.99 μg/g (MC-LR: 1.76 μg/g, MC-RR: 2.51 μg/g, and MC-YR: 7.73 μg/g). Total MC concentrations in L. hoffmeisteri declined after October (2013) and began to increase in May (2014). Between July and October, MC-YR concentration was higher than MC-LR and MC-RR. MC concentrations in L. hoffmeisteri were positively correlated with pH, water temperature, conductivity, chlorophyll a, nitrite and the biomass of Microcystis, and negatively correlated with dissolved oxygen (DO), nitrate, total nitrogen (TN), dissolved total inorganic carbon and the biomass of Bacillariophyta. In addition, MCs in phytoplankton were more strongly correlated with MCs in L. hoffmeisteri than in the water column or sediment. Our results demonstrated that L. hoffmeisteri could accumulate high MC concentrations in the bloom season, which might transfer to the edible zoobenthos and fish through trophic transfer, thereby posing a significant health threat to humans.
显示更多 [+] 显示较少 [-]Titanium dioxide nanoparticles (100–1000 mg/l) can affect vitamin E response in Arabidopsis thaliana
2016
Szymańska, Renata | Kołodziej, Karolina | Ślesak, Ireneusz | Zimak-Piekarczyk, Paulina | Orzechowska, Aleksandra | Gabruk, Michał | Żądło, Andrzej | Habina, Iwona | Knap, Wiesław | Burda, Květoslava | Kruk, Jerzy
In the present study we analyze the effect of seed treatment by a range of nano-TiO2 concentrations on the growth of Arabidopsis thaliana plants, on the vitamin E content and the expression of its biosynthetic genes, as well as activity of antioxidant enzymes and lipid peroxidation. To conduct the mechanistic analysis of nano-TiO2 on plants growth and antioxidant status we applied nanoparticles concentrations that are much higher than those reported in the environment. We find that as the concentration of nano-TiO2 increases, the biomass, and chlorophyll content in 5-week-old Arabidopsis thaliana plants decrease in a concentration dependent manner. In opposite, higher nano-TiO2 concentration enhanced root growth. Our results indicate that a high concentration of nano-TiO2 induces symptoms of toxicity and elevates the antioxidant level. We also find that the expression levels of tocopherol biosynthetic genes were either down- or upregulated in response to nano-TiO2. Thermoluminescence analysis shows that higher nano-TiO2 concentrations cause lipid peroxidation. To the best of our knowledge, this is the first report concerning the effect of nano-TiO2 on vitamin E status in plants. We conclude that nano-TiO2 affects the antioxidant response in Arabidopsis thaliana plants. This could be an effect of a changes in vitamin E gene expression that is diminished under lower tested nano-TiO2 concentrations and elevated under 1000 μg/ml.
显示更多 [+] 显示较少 [-]Genotoxic effects of cadmium and influence on fitness components of Lymantria dispar caterpillars
2016
The current study extends our previous findings concerning the sensitivity of Lymantria dispar larvae to cadmium in light of ecotoxicological risk assessment. Here we report the results of the comet assay performed for the first time on this species. We examined the chronic effects of two cadmium concentrations (50 and 100 μg Cd/g dry food) on DNA integrity and haemocyte viability, as well as on fitness-related traits (larval mass and development duration parameters). All parameters were assessed individually and then used to calculate the integrated biomarker response (IBR) index. Egg-masses of L. dispar were collected from two locations in Serbia - the uncontaminated Homolje mountains and a metal-polluted area near Bor copper mines, smelter and refinery. Distinctive patterns in the response of these populations to cadmium exposure were noticed. In haemocytes of larvae from the pollution-free location both cadmium treatments increased the level of DNA damage, although in a similar range. Haemocyte viability and larval mass were reduced, while duration of the fourth instar and total development time were prolonged in a concentration-dependent manner. Cadmium tolerance was noticeable in the population from the metal-contaminated site at all organizational levels. Nevertheless, haemocyte viability in that population was reduced by the stronger treatment. Haemocyte viability was recognized as a promising biomarker due to the evident response of both populations to dietary cadmium. Genotoxicity, fitness-related traits and the IBR index could be used for biomonitoring of sensitive populations not previously exposed to metals.
显示更多 [+] 显示较少 [-]Isoprenoids emission in Stipa tenacissima L.: Photosynthetic control and the effect of UV light
2016
Guidolotti, Gabriele | Rey, Ana | Medori, Mauro | Calfapietra, Carlo
Fluxes of CO2 and isoprenoids were measured for the first time in Stipa tenacissima L (alfa grass), a perennial tussock grass dominant in the driest areas of Europe. In addition, we studied how those fluxes were influenced by environmental conditions, leaf ontogeny and UV radiation and compared emission rates in two contrasting seasons: summer when plants are mostly inactive and autumn, the growing season in this region. Leaf ontogeny significantly affected both photosynthesis and isoprenoids emission. Isoprene emission was positively correlated with photosynthesis, although a low isoprene emission was detected in brown leaves with a net carbon loss. Moreover, leaves with a significant lower photosynthesis emitted only monoterpenes, while at higher photosynthetic rates also isoprene was produced. Ambient UV radiation uncoupled photosynthesis and isoprene emission.It is speculated that alfa grass represent an exception from the general rules governing plant isoprenoid emitters.
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