Increased tropospheric ozone (O₃) concentrations in boreal forests affect the emission of biogenic volatile organic compounds (BVOCs), which play crucial roles in biosphere-atmosphere feedbacks. Although it has been well documented that BVOC emissions are altered in response to elevated O₃, consequent effects on the carbon budget have been largely unexplored. Here, we studied the effects of elevated O₃ (80 nmol mol⁻¹) on diurnal variation of BVOC emissions and gas exchange of CO₂ from above- and belowground parts of Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) and further investigated effects on the carbon budget. In spring, elevated O₃ decreased BVOC emissions and net photosynthesis rate (Pn) from above-ground parts of both species. As BVOC emissions have a causal relationship with dormancy recovery, O₃-induced decreases in BVOC emissions indicated the inhibition of dormancy recovery. Contrary to the spring results, in summer BVOC emissions from aboveground parts were increased in response to elevated O₃ in both species. Decreases in Pn indicated O₃ stress. O₃-induced monoterpene emissions from aboveground were the main volatile defense response. Elevated O₃ had little effect on BVOC emissions from belowground parts of either species in spring or summer. In spring, elevated O₃ decreased the proportion of carbon emitted as BVOCs relative to that assimilated by photosynthesis (the proportion of BVOC-C loss) at the soil-plant system levels in both species. In summer, elevated O₃ resulted in a net CO₂–C loss at the soil-plant system level of Scots pine. During this process, O₃-induced BVOC-C loss can represent a significant fraction of carbon exchange between the atmosphere and Scots pine. In Norway spruce, the effects of O₃ were less pronounced. The current results highlight the need for prediction of BVOC emissions and their contributions to the carbon budget in boreal forests under O₃ stress.

Ammonium phosphate (AP), phosphoric acid (PC), and potassium phosphate (TKP) were used for the modification of biochar for enhanced heavy metal passivation in soil. The effect of various phosphorus (P) precursors on adsorption-related properties, P speciation distribution pattern, and the passivation mechanism was investigated by BET, FTIR, XRD, XPS, and ³¹P NMR analysis. The mobility and bio-availability of cadmium (Cd) were studied by extraction experiments, and the P release kinetics was also determined. Results showed that the immobilization efficiency of Cd (II) by biochars followed the order: TKP-BC > PC-BC > AP-BC > BC, and TKP-BC reduced available Cd content by 81% treated with 2% addition. The P speciation shows a significant effect on the P-enriched biochars’ passivation performance, especially orthophosphate, which is essential for the immobilization of Cd²⁺ by forming phosphate precipitation. Pyrophosphate and orthophosphate monoester in AP-BC and PC-BC can promote Cd²⁺ passivation via the formation of P–Cd complexes or organometallic chelates. It is also shown that PC-BC has the lowest P release rate while TKP-BC has the highest percentage of P (15.50%) remaining in the biochar. The results may contribute to the development of modified biochar for soil remediation based on P-related technologies.

Despite the importance of bioavailability for organic chemical bioaccumulation by terrestrial and benthic invertebrates, the principles of bioavailability for organic chemical bioaccumulation remain poorly understood. Here we use large-scale databases with contrasting geographic, compound and organism coverage (from 925 sites, 446 compounds and 184 invertebrate species), and report that bioavailability for organic chemical bioaccumulation follows the power law. It represents that the internal concentration of organic chemicals is the composite power function of the lipid fraction of invertebrates, bulk site concentration of compounds, and organic carbon content of soils/sediments. This law directly links environmental exposures and body burdens of organic chemicals in contaminated sites, and provides a method for enabling case-specific risk assessments of a vast number of organic chemicals and contaminated sites. Our findings may pave the way for translating bioavailability knowledge into risk-oriented regulation of organic chemicals and contaminated sites.

Evidence about the adverse effects of methamphetamine (METH) on invertebrates is scarce. Hence, C. elegans, a representative invertebrate model, was exposed to METH at environmental levels to estimate chronic and transgenerational toxicity. The results of chronic exposure were integrated into an underlying toxicity framework of METH in invertebrates (e.g., benthos) at environmentally relevant concentrations. The induction of cellular oxidative damage-induced apoptosis and fluctuation of ecologically important traits (i.e., feeding and locomotion) might be attributed by the activation of the longevity regulating pathway regulated by DAF-16/FOXO, and detoxification by CYP family enzymes. The adverse effects to the organism level included impaired viability and decreased fecundity. The results from transgenerational exposure elucidated the cumulative METH-induced damage in invertebrates. Finally, a new risk assessment method named toxicity indicator sensitivity distribution (TISD) analysis was proposed by combining multiple toxicity indicator test data (ECₓ) to derive the hazardous concentration for 10% indicators (C₁₀) of one species. The risk quotient (RQ) values calculated by measured environmental concentrations and C₁₀ in southern China, southeastern Australia, and the western US crossed the alarm line (RQ = 5), suggesting a need for long-term monitoring.

Reservoirs are an important type of drinking water source for megacities, while lots of reservoirs are threatened by odor problems during certain seasons. The influencing factors of odor compounds in reservoirs are still unclear. During August 2019, a nationwide survey investigating the distribution of odor compounds in reservoirs used as drinking water sources was conducted on seven reservoirs. 2-methylisoborneol (2-MIB) and geosmin were detected in almost every reservoir, and some odor compound concentrations even exceeded the odor threshold concentration. The average concentration of 2-MIB was 2.68 ng/L, and geosmin was 3.63 ng/L. The average chlorophyll a concentration was 8.25 μg/L. The dominant genera of phytoplankton in these reservoirs belonged to cyanobacteria and diatom. Statistical analysis showed that odor compound concentration was significantly related to the chlorophyll a concentration and indicated that the odor compounds mainly came from phytoplankton. The concentration of odor compounds in the euphotic zone was significantly related to phytoplankton species and biomass. Therefore, the odor compound concentrations in the subsurface chlorophyll maxima layer was generally higher than in the surface layer. However, the odor compounds in the hypolimnion layer were related to the density current. This research suggests that both phytoplankton proliferation events and heavy storm events are important risk factors increasing odor compounds in reservoirs. Control of algal bloom, in-situ profile monitoring system and depth-adjustable pumping system will greatly reduce the risk of odor problems in reservoirs using as water supplies for large cities.

Several environmental pollutants, including pesticides, herbicides and persistent organic pollutants play an important role in the development of chronic diseases. However, most studies have examined environmental pollutants toxicity in target organisms or using a specific toxicological test, losing the real effect throughout the ecosystem. In this sense an integrative environmental risk of pollutants assessment, using different model organisms is necessary to predict the real impact in the ecosystem and implications for target and non-target organisms.The objective of this study was to use alachlor, a chloroacetanilide herbicide responsible for chronic toxicity, to understand its impact in target and non-target organisms and at different levels of biological organization by using several model organisms, including membranes of dipalmitoylphosphatidylcholine (DPPC), rat liver mitochondria, bacterial (Bacillus stearothermophilus), plant (Lemna gibba) and mammalian cell lines (HeLa and neuro2a).Our results demonstrated that alachlor strongly interacted with membranes of DPPC and interfered with mitochondrial bioenergetics by reducing the respiratory control ratio and the transmembrane potential. Moreover, alachlor also decreased the growth of B. stearothermophilus and its respiratory activity, as well as decreased the viability of both mammalian cell lines. The values of TC₅₀ increased in the following order: Lemna gibba < neuro2a < HeLa cells < Bacillus stearothermophilus. Together, the results suggest that biological membranes constitute a putative target for the toxic action of this lipophilic herbicide and point out the risks of its dissemination on environment, compromising ecosystem equilibrium and human health.

It is necessary to understand the interactions between different pesticides in ecotoxicology because pesticides never appear as individual compounds but rather in combinations with other compounds. In this study, we planned to explicate the combined toxic effect of myclobutanil (MYC) and thiamethoxam (THI) on the zebrafish (Danio rerio) by adopting multiple biomarkers. Results unraveled that the 96-h LC₅₀ values of MYC to D. rerio at various life phases ranged from 5.2 to 10.3 mg L⁻¹, which were lower than those of THI ranging from 147 to 246 mg L⁻¹. Combinations of MYC and THI exhibited synergetic toxicity to zebrafish embryos. The activities of antioxidative enzymes (T-SOD, Cu/Zn-SOD and POD) and detoxification enzyme (GST) were obviously varied in most of the MYC, THI and combined exposures compared to the control. The mRNA expressions of eight genes (Cu-sod, cas3, il-8, cxcl, erα, crh, cyp17 and dio1) involved in antioxidation, apoptosis, immunity and endocrine were obviously altered in the combined exposure of MYC and THI compared to their individual exposures. Our findings hinted the threats when YMC and THI co-existed, which would be beneficial for the risk assessments of pesticide mixtures.

Effects of source inhomogeneity on pollutant dispersion from a cubic building array are investigated as a function of the external wind direction. Using building-resolving large-eddy simulation, it is found that the results depend strongly on the source location and source uniformity inside a near-field region defined by a radius of homogenisation (RAD) based on the spatial autocorrelation of the pollutant concentration. The sensitivity of the RAD to the source location changes abruptly around 30° and is greatly reduced for wind angles between 30 and 45°, in agreement with velocity statistics and the mean horizontal streamlines. The optimal source allocation, which is a proxy for emissions from time-dependent traffic, also changes around 30°. This work clarifies the relationship between inhomogeneous velocity and pollutant statistics and may be applied to the formulation of traffic control policy.

Indoor air quality is critically important to the human as people spend most time indoors. Indoor PM₂.₅ is related to the outdoor levels, but more directly influenced by internal sources. Severe household air pollution from solid fuel use has been recognized as one major risk for human health especailly in rural area, however, the issue is significantly overlooked in most national air quality controls and intervention policies. Here, by using low-cost sensors, indoor PM₂.₅ in rural homes burning coals was monitored for ~4 months and analyzed for its temporal dynamics, distributions, relationship with outdoor PM₂.₅, and quantitative contributions of internal sources. A bimodal distribution of indoor PM₂.₅ was identified and the bimodal characteristic was more significant at the finer time resolution. The bimodal distribution maxima were corresponding to the emissions from strong internal sources and the influence of outdoor PM₂.₅, respectively. Indoor PM₂.₅ was found to be correlated with the outdoor PM₂.₅, even though indoor coal combustion for heating was thought to be predominant source of indoor PM₂.₅. The indoor-outdoor relationship differed significantly between the heating and non-heating seasons. Impacts of typical indoor sources like cooking, heating associated with coal use, and smoking were quantitatively analyzed based on the highly time-resolved PM₂.₅. Estimated contribution of outdoor PM₂.₅ to the indoor PM₂.₅ was ~48% during the non-heating period, but decreased to about 32% during the heating period. The contribution of indoor heating burning coals comprised up to 47% of the indoor PM₂.₅ during the heating period, while the other indoor sources contributed to ~20%. The study, based on a relatively long-term timely resolved PM₂.₅ data from a large number of rural households, provided informative results on temporal dynamics of indoor PM₂.₅ and quantitative contributions of internal sources, promoting scientific understanding on sources and impacts of household air pollution.