Genotoxic agents may exert damage that may be active through following generations. Micronuclei are cytoplasmic chromatin masses outside the main cell nucleus, indicators of chromosomal damage. This study applied micronuclei test to evaluate genetic damage of fish in three breeding and nursery areas of commercially important fishes exposed to differing degrees of anthropogenic impact: Pando, Solís Chico and Solís Grande sub-estuaries. The micronuclei test revealed differences in the presence and concentrations of genotoxic components in these sub-estuaries. Fish in the urban and industrialized Pando sub-estuary presented the highest percentages of micronuclei. The absence of seasonal changes in micronuclei in Pando fish could be explained by physical characteristics of the sub-estuary and by regional weather conditions. Odontesthes argentinensis (Valenciennes, 1835) was the species most susceptible to genotoxic components. It is recommended the use of a battery of biomarkers to better explain the health of organisms in that important nursery area.

To assess the potential for nutritional exploitation of caged-fish-derived waste through the use of extractive co-cultured species in a pilot system for an integrated multi-trophic aquaculture (IMTA), we compared their C and N stable isotope ratios with those of uncultured macroinvertebrates in and around the system. Black rockfish were co-cultured with sea cucumber, oyster, and two macroalgae as extractive species. Isotope signatures of the co-cultured sea cucumber at the IMTA site differed from those at the control site, indicating their assimilation of aquaculture wastes. In contrast, δ13C and δ15N of individual taxa of the cultured oyster and uncultured invertebrates were consistent between sites, suggesting a minor contribution of the aquaculture waste to benthic and pelagic food chains in and around the IMTA system. These results provide evidence of the suitability of using sea cucumber as an extractive species to reduce the impact of a monoculture system on the ambient environment.

Compositions, changes and biomass of attached Ulva species on Porphyra rafts along the radial sandbank in the Yellow Sea were investigated, and potential contributions to green tides was analyzed. Ulva prolifera, Ulva flexuosa and Ulva linza were all appeared throughout the investigated period. U. prolifera and U. flexuosa dominated attached Ulva population on Porphyra rafts. Attached Ulva species biomass showed obviously spatial and temporal variations. Temperature, Ulva microscopic propagules and human activities were main factors to influence attached Ulva species biomass. The total attached Ulva species biomass was more than 20,000 fresh weight tons in April, and the green tide causative species U. prolifera accounted 51.03% in April 2013 before green tides occurred. The high biomass of attached Ulva species would contribute most to green tides in the Yellow Sea. But how attached Ulva species on Porphyra rafts contributing to green tides in the Yellow Sea should be further studied.

The disturbance and subsequent dispersion of sediment arising from aggregate dredging results in increases in suspended sediment concentrations and, potentially, settlement of fine sediment or sand onto the bed, which may both cause adverse effects on local ecology. This subject is one area which has seen much research over many years and this paper sets out to synthesise some basic general conclusions for use when assessing the significance of planned operations. The literature detailing the dispersion of fine sediment plumes, and the longer term dispersion of sand released through the dredging process, is scrutinised, and in some cases re-evaluated, and used to identify an evidence-based footprint of potential impact.

Rice (Oryza sativa L.) grown on Zn mine waste contaminated soils has caused unequivocal Cd effects on kidney and occasional bone disease (itai-itai) in subsistence rice farmers, but high intake of Cd from other foods has not caused similar effects. Research has clarified two important topics about how Cd from mine waste contaminated rice soils has caused Cd disease: (1) bioaccumulation of soil Cd into rice grain without corresponding increase in Zn, and (2) subsistence rice diets potentiate Cd absorption/bioavailability and risk to farm families. Absorption of Cd by rice roots occurs on the NRAMP5 Mn²⁺ transporter. Although other transporters can influence Cd uptake-transport to shoots and grain, making NRAMP5 null greatly reduces grain Cd. Zn²⁺ has little ability to inhibit Cd²⁺ transfer in rice but clearly inhibits Cd uptake in other plant species. The bioavailability of dietary Cd is increased for subsistence rice diets. Research has identified that low levels and bioavailability of Zn and Fe in polished rice grain cause upregulation of Cd absorption on the Fe²⁺ transporter of duodenum cells (DMT1). Added dietary Zn can also inhibit intestinal Cd absorption somewhat. Nutritional stress (Fe, Zn deficiency) in humans consuming subsistence rice diets thus promotes Cd accumulation and adverse effects. No other dietary (crop) Cd exposure has caused unequivocal Cd-induced renal proximal tubular dysfunction (the first adverse Cd effect) in humans. Recognition of the very unusual nature of Cd risk from rice compared to other crops should be taken into account in setting international limits of Cd in rice and other foods.

The aims of this study were to investigate the presence of Staphylococcus aureus and staphylococcal enterotoxins, as well as Salmonella spp. and to determine the antimicrobial susceptibilities of the isolates from fish samples. A total of 100 fish samples were analysed consisting of 30 anchovy, 35 trout and 35 sea bream. The presence of SEs was detected using ELISA and its genes confirmed by mPCR. Also, S. aureus and Salmonella spp. were detected in 9 (9%) and 5 (5%) samples, respectively. None of the S. aureus isolates had SEs and SEs genes. The resistance rates of the S. aureus isolates to erythromycin, tetracycline, and penicillin G were found to be 33% while Salmonella spp. isolates were resistant to trimethoprim-sulfamethoxazole, gentamicin and neomycine in 20%, 20% and 80%, respectively of the samples. It is of utmost important for public health that retail fish markets need to use hygienic practices in handling and processing operations.

Marine litter is a significant environmental problem inherently linked to individuals’ purchasing, use and disposal behaviour. This research examined 176 British schoolchildren’s (aged 8–13years) baseline marine litter understanding and self-reported actions, and tested the impact of an educational intervention. All children participated in the educational intervention and completed a pre- and post-intervention questionnaire. At baseline, children were quite concerned about marine litter and recognised some of the causes and impacts of the problem. Children also reported taking a number of actions to help solve the problem. After the intervention, children were significantly more concerned, had a better understanding of the causes and negative impacts, and reported engaging in more actions to reduce the potential causes of marine litter. Understanding the perceptions and behaviours of children is crucial as they represent current and future actors and a potentially important source of social influence among their peers, parents and community.

Aerosols are among the most interesting topics investigated by researchers because of their complicated characteristics and poor quantification. Moreover, significant uncertainties are associated with changes in the Earth's radiation budget. Previous studies have shown numerous difficulties and challenges in quantifying aerosol influences. In addition, the heterogeneity from aerosol loading and properties, including spatial, temporal, size, and composition features, presents a challenge. In this study, we investigated aerosol characteristics over two regions with different environmental conditions and aerosol sources. The study sites are Penang and Kuching in Malaysia, where a ground–based AErosol RObotic NETwork (AERONET) sun–photometer was deployed. The types of aerosol, such as biomass burning, urban/industrial, marine, and dust aerosols, for both study sites were identified by analyzing aerosol optical depth and angstrom exponent. Seasonal monsoon variation results in different aerosol optical properties, characteristics, and types of aerosols that are dominant in Penang and Kuching. Seasonal monsoon flow trend patterns from a seven–day back–trajectory frequency plotted by the Hybrid Single–Particle Lagrangian Integrated Trajectory model illustrated the distinct origins of trans–boundary aerosol sources. Finally, we improved our findings in Malaysian sites using the AERONET data from Singapore and Indonesia. Similarities in the optical properties of aerosols and the distribution types (referred to as homogeneous aerosol) were observed in the Penang–Singapore and the Kuching–Pontianak sites. The dominant aerosol distribution types were completely different for locations in the western (Penang–Singapore) and eastern (Kuching–Pontianak) parts of the South China Sea. This is a result of spatial and temporal heterogeneity. The spatial and temporal heterogeneities for the western and eastern portions of South China Sea provide information on the natural or anthropogenic processes that take place.

The open top chamber (OTC) method was used in a farmland to study the influence of different levels of O3 concentrations (40 ppb, 80 ppb and 120 ppb) on the enzymatic activity and metabolite contents of the antioxidation system of the winter wheat leaves during the jointing, heading and milk stage. The protective effect of exogenous spermidine (Spd) against the antioxidation of winter wheat under the O3 stress was investigated. With the increasing O3 concentrations and fumigation time, the injuries of the winter wheat leaves were observed to be more serious. For instance, when the O3 concentration reached 120 ppb, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and nitrate reductase (NR) in the jointing stage decreased by 50.3%, 64.9%, 75.5% and 92.9%, respectively; peroxidase (POD) and glutathione reductase (GR) increased by 45.1% and 80.5%, respectively; the contents of malondialdehyde (MDA), ascorbic acid (AsA) and reduced glutathione (GSH) increased by 314.3%, 8.4% and 31.7%, respectively; and the soluble protein (SP) content decreased by 47.5%. The O3 stress also had significant impact on the contents of proline (Pro), NO3––N and NH4+–N of the winter wheat leaves. During the heading stage, when the O3 concentration was 40 ppb and 80 ppb, the content of Pro was 163.9% and 173.2% higher than that in the control group, respectively. But under 120 ppb, it was decreased by 42.4%. Exogenous application of Spd increased the activities of SOD, POD, CAT, APX and GR, as well as the contents of GSH and SP, but decreased the contents of MDA and AsA. This indicates that Spd is an effective antioxidant to relieve the O3 stress on winter wheat leaves, thereby might be applicable to protect winter wheat from the harm of O3.

Mercury and selenium were assessed in the sailfish, Istiophorus platypterus, from the Eastern Pacific. Sixty-seven individuals were sampled, muscle, liver, kidney, gonads and the prey found in the stomach contents were isolated during fishing 2011–2013 tournaments. Hg exhibited the following pattern (μgg−1 wet weight): liver (0.57±0.07)>muscle (0.56±0.04)>kidney (0.44±0.08)>gonad (0.14±0.01). The maximum concentration of Se was found in kidneys (14.1±1.9μgg−1), and the minimum in muscles (0.67±0.03μgg−1). High Se:Hg ratios were found for muscle (4.1±0.3), kidney (132.4±12.1), liver (54.0±4.4) and gonads (88.2±7.9); Hg:Se molar ratios were several orders of magnitude lower (muscle<0.4 and liver, kidney and gonad<0.03). Sailfish feed mainly on fishes and cephalopods with low Hg levels (<0.13μgg−1), these results indicate biomagnification of Hg and Se. The muscle of I. platypterus should be consumed (according the provisional tolerable weekly intake) by people cautiously so as not to exceed the recommended intake of 215g per week.