Identifying the adverse impacts of pesticide exposure is essential to guide regulations that are protective of wildlife and human health. Within rice ecosystems, amphibians are valuable indicators because pesticide applications coincide with sensitive reproductive and developmental life stages. We conducted two experiments using wild cane toads (Rhinella marina) to test 1) whether environmentally relevant exposure to a commercial formulation of butachlor, an acetanilide herbicide used extensively in rice, affects amphibian development and 2) whether cane toad tadpoles are capable of acclimatizing to sub-lethal exposure. First, we exposed wild cane toads to 0.002, 0.02, or 0.2 mg/L of butachlor (Machete EC), during distinct development stages (as eggs and hatchlings, as tadpoles, or continuously) for 12 days. Next, we exposed a subset of animals from the first experiment to a second, lethal concentration and examined survivorship. We found that cane toads exposed to butachlor developed slower and weighed less than controls, and that development of the thyroid gland was affected: exposed individuals had smaller thyroid glands and thyrocyte cells, and more individual follicles. Analyses of the transcriptome revealed that butachlor exposure resulted in downregulation of transcripts related to metabolic processes, anatomic structure development, immune system function, and response to stress. Last, we observed evidence of acclimatization, where animals exposed to butachlor early in life performed better than naïve animals during a second exposure. Our findings indicate that the commercial formulation of butachlor, Machete EC, causes thyroid endocrine disruption in vertebrates, and suggest that exposure in lowland irrigated rice fields presents a concern for wildlife and human health. Furthermore, we establish that developmental assays with cane toads can be used to screen for adverse effects of pesticides in rice fields.

Fertilizer, though one of the most essential inputs for increasing agricultural production, is a leading cause of nitrous oxide emissions from agriculture, contributing significantly to global warming. Therefore, understanding factors affecting farmers’ use of fertilizers is crucial to develop strategies to improve its efficient use and to minimize its negative impacts. Using data from 2528 households across the Indo-Gangetic Plains in India, Nepal, and Bangladesh, this study examines the factors affecting farmers’ use of organic and inorganic fertilizers for the two most important cereal crops – rice and wheat. Together, these crops provide the bulk of calories consumed in the region. As nitrogen (N) fertilizer is the major source of global warming and other environmental effects, we also examine the factors contributing to its overuse. We applied multiple regression models to understand the factors influencing the use of inorganic fertilizer, Heckman models to understand the likelihood and intensity of organic fertilizer (manure) use, and a probit model to examine the over-use of N fertilizer. Our results indicate that various socio-economic and geographical factors influence the use of organic and inorganic fertilizers in rice and wheat. Across the study sites, N fertilizer over-use is the highest in Haryana (India) and the lowest in Nepal. Across all locations, farmers reported a decline in manure application, concomitant with a lack of awareness of the principles of appropriate fertilizer management that can limit environmental externalities. Educational programs highlighting measures to improving nutrient-use-efficiency and reducing the negative externalities of N fertilizer over-use are proposed to address these problems.

Fertilizer, though one of the most essential inputs for increasing agricultural production, is a leading cause of nitrous oxide emissions from agriculture, contributing significantly to global warming. Therefore, understanding factors affecting farmers’ use of fertilizers is crucial to develop strategies to improve its efficient use and to minimize its negative impacts. Using data from 2528 households across the Indo-Gangetic Plains in India, Nepal, and Bangladesh, this study examines the factors affecting farmers’ use of organic and inorganic fertilizers for the two most important cereal crops – rice and wheat. Together, these crops provide the bulk of calories consumed in the region. As nitrogen (N) fertilizer is the major source of global warming and other environmental effects, we also examine the factors contributing to its overuse. We applied multiple regression models to understand the factors influencing the use of inorganic fertilizer, Heckman models to understand the likelihood and intensity of organic fertilizer (manure) use, and a probit model to examine the over-use of N fertilizer. Our results indicate that various socio-economic and geographical factors influence the use of organic and inorganic fertilizers in rice and wheat. Across the study sites, N fertilizer over-use is the highest in Haryana (India) and the lowest in Nepal. Across all locations, farmers reported a decline in manure application, concomitant with a lack of awareness of the principles of appropriate fertilizer management that can limit environmental externalities. Educational programs highlighting measures to improving nutrient-use-efficiency and reducing the negative externalities of N fertilizer over-use are proposed to address these problems. | 51480-51496

Fertilizer, though one of the most essential inputs for increasing agricultural production, is a leading cause of nitrous oxide emissions from agriculture, contributing significantly to global warming. Therefore, understanding factors affecting farmers’ use of fertilizers is crucial to develop strategies to improve its efficient use and to minimize its negative impacts. Using data from 2528 households across the Indo-Gangetic Plains in India, Nepal, and Bangladesh, this study examines the factors affecting farmers’ use of organic and inorganic fertilizers for the two most important cereal crops – rice and wheat. Together, these crops provide the bulk of calories consumed in the region. As nitrogen (N) fertilizer is the major source of global warming and other environmental effects, we also examine the factors contributing to its overuse. We applied multiple regression models to understand the factors influencing the use of inorganic fertilizer, Heckman models to understand the likelihood and intensity of organic fertilizer (manure) use, and a probit model to examine the over-use of N fertilizer. Our results indicate that various socio-economic and geographical factors influence the use of organic and inorganic fertilizers in rice and wheat. Across the study sites, N fertilizer over-use is the highest in Haryana (India) and the lowest in Nepal. Across all locations, farmers reported a decline in manure application, concomitant with a lack of awareness of the principles of appropriate fertilizer management that can limit environmental externalities. Educational programs highlighting measures to improving nutrient-use-efficiency and reducing the negative externalities of N fertilizer over-use are proposed to address these problems.

Endocrine-disrupting chemicals (EDCs) in diet are a health concern and their monitoring in food has been introduced in the European Union. In developing countries, EDC dietary exposure data are scarce, especially from areas perceived as pollution hotspots, including industrialized countries like India. Several persistent organic pollutants (POPs) act as EDCs and pose a pressure to human health mainly through dietary exposure. In the present study a range of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), dioxins and furans were measured in several food items collected in an Indian urban (Delhi) and a rural area (Dehradun). Food basket contamination data were used to estimate dietary exposure and compare it with that of the average European population estimated from available monitoring data. All targeted contaminants were found in most food items, especially in dairies and meat products. OCPs were the main contributors. Food supplied to Delhi's markets had higher contamination than that supplied to the peri-urban market in Dehradun. Despite looser control and restrictions, Indian dietary exposure of OCPs and PBDEs were comparable with that of Europe and were lower for PCBs and dioxins. Higher meat consumption in Europe only partly explained this pattern which was driven also by the higher residues in some European food items. A substantial part of endocrine disrupting potential in the diet derives from food and animal feeds internationally traded between developed and developing countries. With increasingly globalized food systems, internationally harmonized policies on EDC in food can lead to better protection of health in both these contexts. | publishedVersion

Endocrine-disrupting chemicals (EDCs) in diet are a health concern and their monitoring in food has been introduced in the European Union. In developing countries, EDC dietary exposure data are scarce, especially from areas perceived as pollution hotspots, including industrialized countries like India. Several persistent organic pollutants (POPs) act as EDCs and pose a pressure to human health mainly through dietary exposure. In the present study a range of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), dioxins and furans were measured in several food items collected in an Indian urban (Delhi) and a rural area (Dehradun). Food basket contamination data were used to estimate dietary exposure and compare it with that of the average European population estimated from available monitoring data. All targeted contaminants were found in most food items, especially in dairies and meat products. OCPs were the main contributors. Food supplied to Delhi's markets had higher contamination than that supplied to the peri-urban market in Dehradun. Despite looser control and restrictions, Indian dietary exposure of OCPs and PBDEs were comparable with that of Europe and were lower for PCBs and dioxins. Higher meat consumption in Europe only partly explained this pattern which was driven also by the higher residues in some European food items. A substantial part of endocrine disrupting potential in the diet derives from food and animal feeds internationally traded between developed and developing countries. With increasingly globalized food systems, internationally harmonized policies on EDC in food can lead to better protection of health in both these contexts. | publishedVersion

Endocrine-disrupting chemicals (EDCs) in diet are a health concern and their monitoring in food has been introduced in the European Union. In developing countries, EDC dietary exposure data are scarce, especially from areas perceived as pollution hotspots, including industrialized countries like India. Several persistent organic pollutants (POPs) act as EDCs and pose a pressure to human health mainly through dietary exposure. In the present study, a range of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), dioxins and furans were measured in several food items collected from Indian urban (Delhi) and peri-urban (Dehradun) areas. Food basket contamination data were used to estimate EDC dietary exposure and compare it with that of the average European population estimated from available monitoring data. All the target contaminants were found in most food items, especially in dairies and meat products. OCPs were the main contributers to the measured EDC contamination. Food supplied to Delhi's markets had higher EDC contamination than that supplied to the peri-urban market in Dehradun. Despite lax compliance and control measures, Indian dietary exposure of OCPs and PBDEs were comparable with that of Europe and were lower for PCBs and dioxins. Higher meat consumption in Europe only partly explained this pattern which was driven also by the higher EDC residues in some European food items. A substantial part of endocrine disrupting potential in the diet derives from food and animal feeds internationally traded between developed and developing countries. With increasingly globalized food systems, internationally harmonized policies on EDC content in food can lead to better protection of health in both these contexts.

Little is known about the occurrence of emerging pollutants (EPs) in waters in the Middle East and North Africa (MENA) region despite the extensive use of low-quality water there. Available data dealing with the sources, occurrence and removal of EPs within the MENA region in different categories of water is collected, presented and analyzed in this literature review. According to the collected database, the occurrence and removal efficiency of EPs in the water matrix in the MENA region is available, respectively, for 13 and six countries of the 18 in total; no available data is registered for the rest. Altogether, 290 EPs have been observed in different water matrices across the MENA countries, stemming mainly from industrial effluents, agricultural practices, and discharge or reuse of treated wastewater (TWW). Pharmaceutical compounds figure among the most frequently reported compounds in wastewater, TWW, surface water, and drinking water. Nevertheless, pesticides are the most frequently detected pollutants in groundwater. Worryingly, 57 cases of EPs have been reported in different fresh and drinking waters, exceeding World Health Organization (WHO) and European Commission (EC) thresholds. Overall, pharmaceuticals, organic compounds, and pesticides are the most concerning EP groups. The review revealed the ineffectiveness of treatment processes used in the region to remove EPs. Negative removals of some EPs such as carbamazepine, erythromycin, and sulfamethoxazole were recorded, suggesting their possible accumulation or release during treatment. This underlines the need to set in place and strengthen control measures, treatment procedures, standards, and policies for such pollutants in the region.

Little is known about the occurrence of emerging pollutants (EPs) in waters in the Middle East and North Africa (MENA) region despite the extensive use of low-quality water there. Available data dealing with the sources, occurrence and removal of EPs within the MENA region in different categories of water is collected, presented and analyzed in this literature review. According to the collected database, the occurrence and removal efficiency of EPs in the water matrix in the MENA region is available, respectively, for 13 and six countries of the 18 in total; no available data is registered for the rest. Altogether, 290 EPs have been observed in different water matrices across the MENA countries, stemming mainly from industrial effluents, agricultural practices, and discharge or reuse of treated wastewater (TWW). Pharmaceutical compounds figure among the most frequently reported compounds in wastewater, TWW, surface water, and drinking water. Nevertheless, pesticides are the most frequently detected pollutants in groundwater. Worryingly, 57 cases of EPs have been reported in different fresh and drinking waters, exceeding World Health Organization (WHO) and European Commission (EC) thresholds. Overall, pharmaceuticals, organic compounds, and pesticides are the most concerning EP groups. The review revealed the ineffectiveness of treatment processes used in the region to remove EPs. Negative removals of some EPs such as carbamazepine, erythromycin, and sulfamethoxazole were recorded, suggesting their possible accumulation or release during treatment. This underlines the need to set in place and strengthen control measures, treatment procedures, standards, and policies for such pollutants in the region.