We describe an innovative method of planting Zostera marina (eelgrass) seeds in which hessian bags filled with high-silted sediments are used as a seed protecting device. Here, we evaluated the effectiveness of the method through a field seed-sowing experiment over a three year period. The suitable seed planting density required by the seeds of Z. marina in this method was also investigated. In the spring following seed distribution, seedling establishment rate of Z. marina subjected to different seed densities of 200–500seedsbag−1 ranged from 16% to 26%. New eelgrass patches from seed were fully developed and well maintained after 2–3years following distribution. The seed planting density of 400seedsbag−1 may be the most suitable for the establishment of new eelgrass patches. Our results demonstrate that seed-based restoration can be an effective restoration tool and the technique presented should be considered for future large-scale Z. marina restoration projects.

Platichthys flesus is often used as a sentinel species to monitor the estuarine water quality. In this study, we carried out an experimental contamination of fish using a PAHs/PCBs mixture, which was designed to mimic the concentrations found in the Seine estuary (C1) and 10 times these concentrations (C2). We used a proteomic approach to understand the molecular mechanisms implied in the response of P. flesus to these xenobiotics. We showed that 54 proteins were differentially accumulated in one or several conditions, which 34 displayed accumulation factors higher than two. 18 of these proteins were identified by MALDI TOF–TOF mass spectrometry. The results indicated the deregulation of oxidative stress- and glutathione metabolism-(GST, GPx) proteins as well as of several proteins belonging to the betaine demethylation pathway and the methionine cycle (BHMT, SHMT, SAHH), suggesting a role for these different pathways in the P. flesus response to chemical contamination.

We assessed the effects of 40 ocean outfalls on adjacent macrobenthic invertebrates. Data were obtained from a review of gray and peer-review literature. Different parameters describing the outfall characteristics were compiled (length, maximum depth, treatment level, flow and organic matter mass discharged). Exposure to wave action was represented by significant wave height. The magnitude of the effect was categorized in three impact levels and classified considering different ecological indicators. A theoretical predictive model was formulated in which the lower the organic matter and the higher the energy of the system, the lower the benthic impact. The main conclusion was that the general pattern of the succession of benthic communities brought about by ocean outfalls fits the model of Pearson–Rosenberg but with some deviations i) the probability of a significant impact is much lower, ii) not all the successional stages occur and, iii) the magnitude of the changes are usually lower.

Plastics and other marine debris affect wildlife through entanglement and by ingestion. We assessed the ingestion of marine debris by seven albatross species in the southwest Atlantic by analyzing stomach contents of birds killed in fisheries. Of the 128 specimens examined, including four Diomedea species (n=78) and three Thalassarche species (n=50), 21 (16.4%) contained 1–4 debris items, mainly in the ventriculus. The most common type was plastic fragments. Debris was most frequent in Diomedea species (25.6%) and, particularly, Diomedea sanfordi (38.9%) and very rare in Thalassarche species (2.0%), presumably reflecting differences in foraging behavior or distribution. Frequency of occurrence was significantly higher in male than female Diomedea albatrosses (39.3% vs. 18.0%). Although levels of accumulated debris were relatively low overall, and unlikely to result in gut blockage, associated toxins might nevertheless represent a health risk for Diomedea albatrosses, compounding the negative impact of other human activities on these threatened species.

The successful management of sustainable coastal environments that are beneficial to both humans and marine ecosystems requires knowledge about factors that are harmful to such environments. Here, we investigated seawater nutrient and carbon parameters between 2010 and 2012 in Kwangyang Bay, Korea, a coastal environment that has been exposed to intensive anthropogenic activities. The data were analyzed using cluster and factor analysis. We found that the biogeochemical cycles of nutrients and carbon were determined by river discharge into the bay and biological activity. However, the impacts of these factors varied both spatially and seasonally. During the past 10years, nutrient loads from the river and industrial complexes to the bay have decreased. The impacts of this decrease are visible in the phosphate concentration, which has fallen to a third of its initial value. We also examined the potential role of atmospheric nitrogen deposition in nitrogen cycling in the study area.

Sole and hake, together with sediments, were collected during two campaigns along the Basque continental shelf to study the utility of two existing histopathological indices for assessing the biological effects of contaminants to implement the European Marine Strategy Framework Directive. Hepatic and gonadal histopathology were measured, and metal and/or organic contaminants were determined in both liver and sediments. Sediments from the Basque continental shelf were found to be moderately impacted by metals but non-impacted by organic compounds. Metal bioaccumulation and histopathological lesions in liver were higher in sole than in hake, although non-specific and early non-neoplastic toxicopathic lesions were observed in both species. No gross alterations were recorded in gonad. The two histopathological indices applied were highly correlated in both organs but the lack of correlation between sediment contamination levels, bioaccumulation and histopathological indices suggests that other factors, rather than pollution alone, are responsible for the biological effects observed.

The Georgia Sea Turtle Center has a mission of conservation based rehabilitation, research, and education. Marine debris is a serious threat to marine species. In an effort to educate local students, the GSTC obtained a grant to provide educational opportunities to local third graders. Third and fourth grade classes in Glynn County, Georgia were offered a Garbage in the Water program and 964 students were reached. After programming, students showed a statistically significant (p<.0001) increase in test scores between the pre and posttests. This success led to repeat funding for additional programming for first grades as well as a formalized relationship with the Glynn County School District. As part of this relationship the Georgia Sea Turtle Center is now the official field trip location for all third grades in the district.

Risk assessments of persistent organic pollutants (POPs) are often based on octanol–water (KOW) partitioning dynamics and may not adequately reflect bioaccumulation in air-breathing organisms. It has been suggested that compounds with low KOW and high octanol–air partitioning (KOA) coefficients have the potential to bioaccumulate in air-breathing organisms, including marine mammals. Here we evaluate differences in concentrations of POPs for two trophically matched Arctic species, spotted seal (Phoca largha) and sheefish (Stenodus leucichthys). We compared concentrations of 108 POPs in matched tissues (liver and muscle) across three ranges of KOW. We found a significant positive correlation between POP concentration and log KOA in spotted seal tissues for low log KOW compounds (log KOW <5.5, p<0.05). This provides further evidence for empirical models and observed bioaccumulation patterns in air-breathing organisms, and highlights the potential for bioaccumulation of these compounds in Arctic marine mammals.

Concentrations of elements (As, Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Se, Sr, V, Zn) were determined in liver, kidneys and bones of Eretmochelys imbricata and Chelonia mydas specimens found stranded along the northern coast of Bahia, Brazil. Results showed that the concentrations of Cd, Cu, Ni and Zn in the liver and kidneys of juvenile C. mydas were the highest found in Brazil. We also observed a significant difference (p<0.05) on the bioaccumulation of trace elements between the two species: Al, Co, Mo, Na and Se in the liver; Al, Cr, Cu, K, Mo, Ni, Pb, Sr and V in the kidneys; and Al, Ba, Ca, Cd, Mn, Ni, Pb, Se, Sr and V in the bones. This study represents the first report on the distribution and concentration of trace elements in E. imbricata in the Brazilian coast.

The concentrations of seven trace metals (Fe, Mn, Cu, Cr, Co, Pb and Zn) in three sediment cores were analysed to assess the depositional trends of metals and their contamination level in the Mandovi estuary, west coast of India. All sediment cores showed enrichment of trace metals in the upper part of core sediments and decrease in concentration with depth, suggesting excess of anthropogenic loading (including mining activities) occurred during the recent past. Scanning electron microscope (SEM) images distinguished the shape, size and structure of particles derived from lithogenic and anthropogenic sources in core sediments. The geo-accumulation index (Igeo) values indicate that Mandovi estuary is ‘moderately polluted’ with Pb, whereas ‘unpolluted to moderately polluted’ with Fe, Mn, Cu, Cr, Co and Zn. The comparative analysis of trace metals revealed that Fe and Mn were highly enriched in the Mandovi estuary compared to all other Indian estuaries.