Perfluoroalkyl substances (PFAS) are a diverse class of manufactured compounds used in a wide range of industrial processes and consumer products and have been detected in human serum worldwide. Previous cross-sectional and cohort studies in humans have suggested exposure to PFAS is associated with a wide array of chronic diseases, including endocrine disruption, developmental health effects, cancer and metabolic changes. We examined the associations between a panel of eight PFAS and indicators of thyroid disruption, kidney function, and body mass index (BMI), all of which were measured at repeated time points (1990–2008) over the course of the study. Participants (N = 210) were selected from the Fernald Community Cohort based on household water supply from a PFAS-contaminated aquifer. In adjusted repeated measures models, we observed several notable associations between serum PFAS and thyroid hormones as well as kidney function as measured by estimated glomerular filtration rate (eGFR). An interquartile (IQR) increase in serum PFOS was associated with a 9.75% (95% CI = 1.72, 18.4) increase in thyroid stimulating hormone. An IQR increase in serum PFNA, PFHxS, and PFDeA was associated with a −1.61% (95% CI = −3.53, −0.59), −2.06% (95% CI = −3.53, −0.59), and −2.20% (95% CI = −4.25, −0.14) change in eGFR, respectively. On the other hand, an IQR increase in serum Me-PFOSA was associated with a 1.53% (95% CI = 0.34, 2.73) increase in eGFR. No significant associations with BMI and serum PFAS were noted. Our findings are in agreement with previous reports that serum PFAS are associated with altered kidney and thyroid function.

Microbial communities play vital roles in the biogeochemistry of nutrients in coastal saltmarshes, ultimately controlling water quality, nutrient cycling, and detoxification. We determined the structure of microbial populations inhabiting coastal saltmarsh sediments from northern Barataria Bay, Louisiana, USA to gain insight into impacts on the biogeochemical cycles affected by Macondo oil from the 2010 Deepwater Horizon well blowout two years after the accident. Quantitative PCR directed toward specific functional genes revealed that oiled marshes were greatly diminished in the population sizes of diazotrophs, denitrifiers, nitrate-reducers to ammonia, methanogens, sulfate-reducers and anaerobic aromatic degraders, and harbored elevated numbers of alkane-degraders. Illumina 16S rRNA gene sequencing indicated that oiling greatly changed the structure of the microbial communities, including significant decreases in diversity. Oil-driven changes were also demonstrated in the structure of two functional populations, denitrifying and sulfate reducing prokaryotes, using nirS and dsrB as biomarkers, respectively. Collectively, the results from 16S rRNA and functional genes indicated that oiling not only markedly altered the microbial community structures, but also the sizes and structures of populations involved in (or regulating) a number of important nutrient biogeochemical cycles in the saltmarshes. Alterations such as these are associated with potential deterioration of ecological services, and further studies are necessary to assess the trajectory of recovery of microbial-mediated ecosystem functions over time in oiled saltmarsh sediment.

Moss (as a reference material) and camphor (Cinnamomum Camphora) leaf, branch bark and bark samples were systematically collected across an urban-rural gradient in Guiyang (SW China) to determine the efficacy of using these bio-indicators to evaluate nitrogen (N) pollution. The tissue N concentrations (0.13%–2.70%) and δ¹⁵N values (−7.5‰ to +9.3‰) of all of these bio-indicators exhibited large spatial variations, as they recorded higher values in urban areas that quickly decreased with distance from the city center; moreover, both soil N concentrations and soil δ¹⁵N values were found no significant differences within each 6 km from the urban to the rural area. This not only suggests that the different N uptake strategies and variety of N responses of these bio-indicators can be reflected by their different susceptibilities to variations in N deposition but also reveals that they are able to indicate that urban N deposition is mostly from traffic and industry (NOₓ-N), whereas rural N deposition is mainly from agriculture (NHₓ-N). Compared to previously collected urban moss and camphor leaf samples, the significantly increased δ¹⁵N values in current urban moss and camphor leaf samples further indicate a greater contribution of NOₓ-N than NHₓ-N to urban N deposition. The feasibility of using the N concentrations and δ¹⁵N values of branch bark and bark as biomarkers of N deposition thus was further confirmed through the comparative use of these bio-indicators. It can be concluded that vascular plant leaves, branch bark and bark can be used as useful biomonitoring tools for evaluating atmospheric N pollution. For further study, quantitative criteria for the practical use of these bio-indicators in response to N deposition should be developed and the differences in the δ¹⁵N values of different plant parts should also be considered, particularly in urban environments that are severely disrupted by atmospheric pollution.

Venlafaxine is an antidepressant and anti-anxiety drug that has been detected in municipal wastewater at low μg/L concentrations. In this study, the nest-defense behavior of adult male fathead minnows (Pimephales promelas) was observed in fish exposed for a full lifecycle to venlafaxine nominal concentrations of 0.88, 8.8, and 88 μg/L (i.e. 1, 9.3, 75 μg/L mean measured concentrations). Nest-defense behaviors quantified were the time taken to contact a dummy intruder fish (on a flexible stick, held near each nest) and the number of contacts made during a 1 min period. In male fathead minnows exposed to venlafaxine over a full lifecycle at environmentally relevant nominal concentrations (i.e. 0.88 and 8.8 μg/L) no significant effects were observed in behavior. However, in males exposed over a full lifecycle to the highest concentration of venlafaxine (i.e. 88 μg/L), nest-defense behaviors were increased in males with empty nests, as shown by the significantly elevated percentage of empty-nest males that made contact with the dummy intruder fish (89%) relative to the lower percentage of contacts (65%) among the Control males (p = 0.046). Lifecycle exposure to high venlafaxine (88 μg/L) caused males to over-protect their empty nests. Environmental venlafaxine concentrations are approximately 70 x lower than this, so it is unlikely that behavioral changes from venlafaxine exposure would occur in the environment. Normal nest defense behaviours in control males varied, depending on whether they were protecting empty nests or nests with eggs. Compared to Control males with empty nests, more Control males with eggs in their nests made contact with the dummy intruder fish (p = 0.014), contact was faster (i.e. <10 s, p = 0.011), and they hit the dummy intruder fish more times in 1 min (p = 0.031) This study is the first to assess reproductive behaviors in fish exposed to an antidepressant over a full lifecycle.

Wind induced flow velocity patterns and associated thermal destratification can drive to hypoxia reduction in large shallow lakes. The effects of wind induced hydrodynamic changes on destratification and hypoxia reduction were investigated at the Meiling bay (N 31° 22′ 56.4″, E 120° 9′ 38.3″) of Lake Taihu, China. Vertical flow velocity profile analysis showed surface flow velocities consistency with the wind field and lower flow velocity profiles were also consistent (but with delay response time) when the wind speed was higher than 6.2 m/s. Wind field and temperature found the control parameters for hypoxia reduction and for water quality conditions at the surface and bottom profiles of lake. The critical temperature for hypoxia reduction at the surface and the bottom profile was ≤24.1C° (below which hypoxic conditions were found reduced). Strong prevailing wind field (onshore wind directions ESE, SE, SSE and E, wind speed ranges of 2.4–9.1 m/s) reduced the temperature (22C° to 24.1C°) caused reduction of hypoxia at the near surface with a rise in water levels whereas, low to medium prevailing wind field did not supported destratification which increased temperature resulting in increased hypoxia. Non-prevailing wind directions (offshore) were not found supportive for the reduction of hypoxia in study area due to less variable wind field. Daytime wind field found more variable (as compared to night time) which increased the thermal destratification during daytime and found supportive for destratification and hypoxia reduction. The second order exponential correlation found between surface temperature and Chlorophyll-a (R²: 0.2858, Adjusted R-square: 0.2144 RMSE: 4.395), Dissolved Oxygen (R²: 0.596, Adjusted R-square: 0.5942, RMSE: 0.3042) concentrations. The findings of the present study reveal the driving mechanism of wind induced thermal destratification and hypoxic conditions, which may further help to evaluate the wind role in eutrophication process and algal blooms formation in shallow water environments.Wind field is the key control factor for thermal destratification and hypoxia reduction. 24.1C° is the critical/threshold temperature for hypoxia, Chlorophyll-a and NH3-N concentrations of the shallow freshwater lake.

With the commercialization of transgenic cotton that expresses Bt (Bacillus thuringiensis) insecticidal proteins, mirid bugs have become key pests in cotton and maize fields in China. Genetically engineered (GE) crops for controlling mirids are unavailable owing to a lack of suitable insecticidal genes. In this study, we developed and validated a dietary exposure assay for screening insecticidal compounds and for assessing the potential effects of insecticidal proteins produced by GE plants on Apolygus lucorum, one of the main mirid pests of Bt cotton and Bt maize. Diets containing potassium arsenate (PA) or the cysteine protease inhibitor E-64 were used as positive controls for validating the efficacy of the dietary exposure assay. The results showed that with increasing concentrations of PA or E-64, A. lucorum larval development time was prolonged and adult weight and fecundity were decreased, suggesting that the dietary exposure assay was useful for detecting the toxicity of insecticidal compounds to A. lucorum. This assay was then used to assess the toxicity of Cry1Ab, Cry1Ac, Cry1F, Cry2Aa, and Cry2Ab proteins, which have been transformed into several crops, against A. lucorum. The results showed that A. lucorum did not show a negative effect by feeding on an artificial diet containing any of the purified Cry proteins. No significant changes in the activities of digestive, detoxifying, or antioxidant enzymes were detected in A. lucorum that fed on a diet containing Cry proteins, but A. lucorum fitness was reduced when the insect fed on a diet containing E-64 or PA. These results demonstrate that A. lucorum is not sensitive to the tested Cry proteins and that the dietary exposure assay is useful for evaluating the toxicity of insecticidal compounds to this species.

Due to their carcinogenic effects, Polycyclic Aromatic Hydrocarbons (PAHs) deposited on urban surfaces are a major concern in the context of stormwater pollution. However, the design of effective pollution mitigation strategies is challenging due to the lack of reliability in stormwater quality modelling outcomes. Current modelling approaches do not adequately replicate the interdependencies between pollutant processes and their influential factors. Using Bayesian Network modelling, this research study characterised the influence of vehicular traffic on the build-up of the sixteen US EPA classified priority PAHs. The predictive analysis was conditional on the structure of the proposed BN, which can be further improved by including more variables. This novel modelling approach facilitated the characterisation of the influence of traffic as a source of origin and also as a key factor that influences the re-distribution of PAHs, with positive or negative relationship between traffic volume and PAH build-up. It was evident that the re-distribution of particle-bound PAHs is determined by the particle size rather than the chemical characteristics such as volatility. Moreover, compared to commercial and residential land uses, mostly industrial land use contributes to the PAHs load released to the environment. Carcinogenic PAHs in industrial areas are likely to be associated with finer particles, while PAHs, which are not classified as human carcinogens, are likely to be found in the coarser particle fraction.

Brassinosteroids (BRs), a group of steroid phytohormones, are involved in multiple aspects of plant growth, development and stress responses. Despite recent studies on BRs-promoted pesticide metabolism in plants, the underlying mechanisms remain poorly understood. Here, we showed that 24-epibrassinolide (EBR) significantly enhanced the expression of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and H2O2 accumulation in the apoplast of chlorothalonil (CHT, a broad spectrum nonsystemic fungicide)-treated tomato plants. Silencing of RBOH1 significantly decreased the efficiency of EBR-induced CHT metabolism. Moreover, the EBR-induced upregulation in the transcripts of glutaredoxin gene GRXS16 was suppressed in RBOH1-silenced plants. Further studies indicated that silencing of GRXS16 compromised EBR-induced increases in glutathione content, activity of glutathione S-transferase (GST) and transcript of GST1, leading to an increase in CHT residue. By contrast, overexpression of tomato GRXS16 enhanced the basal levels of glutathione content and GST activity that eventually decreased CHT residues in transgenic plants. Our results reveal that BR-mediated induction of a modest oxidative burst is essential for the acceleration of glutathione-dependent pesticide metabolism via redox modulators, such as GRXS16. These findings shed new light on the mechanisms of BR-induced pesticide metabolism and thus have important implication in reducing pesticide residues in agricultural products.

Silver, such as silver nanoparticles (AgNPs), has been widely used in commercial products and may be released into the environment. The interaction between Ag deposition and biological systems is raising serious concerns because of one health consideration. Cetaceans, as the top predators of the oceans, may be exposed to Ag/Ag compounds and suffer negative health impacts from the deposition of these compounds in their bodies. In the present study, we utilized autometallography (AMG) to localize the Ag in the liver and kidney tissues of cetaceans and developed a model called the cetacean histological Ag assay (CHAA) to estimate the Ag concentrations in the liver and kidney tissues of cetaceans. Our results revealed that Ag was mainly located in hepatocytes, Kupffer cells and the epithelial cells of some proximal renal tubules. The tissue pattern of Ag/Ag compounds deposition in cetaceans was different from those in previous studies conducted on laboratory rats. This difference may suggest that cetaceans have a different metabolic profile of Ag, so a presumptive metabolic pathway of Ag in cetaceans is advanced. Furthermore, our results suggest that the Ag contamination in cetaceans living in the North-western Pacific Ocean is more severe than that in cetaceans living in other marine regions of the world. The level of Ag deposition in cetaceans living in the former area may have caused negative impacts on their health condition. Further investigations are warranted to study the systemic Ag distribution, the cause of death/stranding, and the infectious diseases in stranded cetaceans with different Ag concentrations for comprehensively evaluating the negative health effects caused by Ag in cetaceans.

Fine particles (PM2.5) samples, collected at Weizhou Island over the Gulf of Tonkin on a daytime and nighttime basis in the spring of 2015, were analyzed for primary and secondary organic tracers, together with organic carbon (OC), elemental carbon (EC), and stable carbon isotopic composition (δ13C) of total carbon (TC). Five organic compound classes, including saccharides, lignin/resin products, fatty acids, biogenic SOA tracers and phthalic acids, were quantified by gas chromatography/mass spectrometry (GC/MS). Levoglucosan was the most abundant organic species, indicating that the sampling site was under strong influence of biomass burning. Based on the tracer-based methods, the biomass-burning-derived fraction was estimated to be the dominant contributor to aerosol OC, accounting for 15.7% ± 11.1% and 22.2% ± 17.4% of OC in daytime and nighttime samples, respectively. In two episodes E1 and E2, organic aerosols characterized by elevated concentrations of levoglucosan as well as its isomers, sugar compounds, lignin products, high molecular weight (HMW) fatty acids and β-caryophyllinic acid, were attributed to the influence of intensive biomass burning in the northern Southeast Asia (SEA). However, the discrepancies in the ratios of levoglucosan to mannosan (L/M) and OC (L/OC) as well as the δ13C values suggest the type of biomass burning and the sources of organic aerosols in E1 and E2 were different. Hardwood and/or C4 plants were the major burning materials in E1, while burning of softwood and/or C3 plants played important role in E2. Furthermore, more complex sources and enhanced secondary contribution were found to play a part in organic aerosols in E2. This study highlights the significant influence of springtime biomass burning in the northern SEA to the organic molecular compositions of marine aerosols over the Gulf of Tonkin.