In this study, the role of Cyperus sp. was evaluated for removal of pollutants from swine wastewater. Vertical-flow pilot scale constructed wetlands (CWs) operating with a hydraulic retention time (HRT) of 72 h were monitored in a greenhouse, located in Viçosa, Brazil. Significant differences were observed for the following parameters: Kjeldahl nitrogen, total phosphorus, alkalinity and electric conductivity, with averages removals of 37.5 and 28.5%, 55.9 and 44.4%, 30.2 and 25.6 and 26.1% and 22.9% (for planted and unplanted CWs, respectively). The rate of dry matter yield from Cyperus sp. was 7.5 g m−2 day−1, and the nutrient uptake capacities were 21.8, 2.1, 14.0 and 0.9 g m−2 of N, P, K and Na, respectively. Evapotranspiration (2.7 mm day−1) was statistically higher in the planted CWs. Plants in the CWs are important for achieving high nutrient removal.

A polyaniline-modified quartz crystal microbalance (QCM) sensor was obtained through immobilizing the polyaniline film on the silver electrode surface of quartz crystal resonator by an electrochemical method. The sensor was studied for detecting the formic acid gas of different concentrations, and the results showed that the resonant frequency of QCM decreased quickly in the beginning and tended to be constant in the end when exposed to formic acid gas. The frequency shifts decreased faster as the concentration of formic acid gas increased. And the frequency shifts of the QCM sensors were found to be linearly related to the concentration of formic acid gas, which might be used to estimate the concentration level of the formic acid gas within the range of experimental concentrations. The result of on-line monitoring test fully indicated that the QCM sensor responded effectively to the increasing concentration of formic acid and had important practical significance and broad application prospect in real-time detection of antique conservation environment in the museum.

Odors, such as the malodorous and toxic hydrogen sulfide (H₂S), are released during leachate collection, storage, and treatment. A full-scale biofilter was applied to treat H₂S emitted from a leachate equalization basin in a sanitary landfill site. The inlet concentration of H₂S was 26.3–213.0 mg m⁻³. In steady state, total removal efficiency was over 90 % in summer and over 80 % in winter. The maximum elimination capacity achieved 9.1 g m⁻³ h⁻¹ at a loading rate of 10.5 g m⁻³ h⁻¹. The biofilter was effective at reducing H₂S. Factors on the level of H₂S inlet concentration and performance of the biofilter were investigated. The H₂S inlet load and removal efficiency relied on ambient and biofilter temperature, respectively. The water containing rate and relative humidity presented seasonal variation, according to which the interval period of irrigation could be optimized. The main product of H₂S degradation was sulfate, and sulfur also could be observed from the biofilter. Spatial and temporal shifts in bacterial community composition in the biofilter were determined by polymerase chain reaction-denaturing gradient gel electrophoresis followed by DNA sequence analysis. The present study revealed a correlation between biofilter performance and bacterial community structure, especially in a real application case.

A batch sorption method was used to study the removal of few toxic metals onto the Late Cretaceous clays of Aleg formation (Coniacian–Lower Campanian system), Tunisia, in single, binary and multi-component systems. The collected clay samples were used as adsorbents for the removal of Pb(II), Cd(II), Cu(II) and Zn(II) from aqueous solutions. Results show that the natural clay samples were mainly composed of silica, alumina, iron and magnesium oxides. N2-adsorption measurements indicated mesoporous materials with modest specific surface area of <71 m2/g. Carbonate minerals were the most influencing parameters for heavy metal removal by natural clays in both single and multi-element systems. The affinity sequence was Pb(II)>Cu(II)>Zn(II)>Cd(II) due to the variable physical properties of the studied metals. The maximum adsorption capacity reached 131.58 mg/g in single systems, but decreased to <50.10 mg/g in mixed systems. In single, binary and muti-element systems, the studied clay samples removed substantial amounts of heavy metals, showing better effectiveness than the relevant previous studies. These results suggest that the studied clay samples of the Late Cretaceous clays from Tunisia can be effectively used as natural adsorbents for the removal of toxic heavy metals in aqueous systems.

Incidental losses of dissolved reactive phosphorus (DRP) to a surface waterbody originate from direct losses during land application of fertilizer, or where a rainfall event occurs immediately thereafter. Another source is the soil. One way of immobilising DRP in runoff before discharge to a surface waterbody, is to amend soil within the edge of field area with a high phosphorus (P) sequestration material. One such amendment is iron ochre, a by-product of acid mine drainage. Batch experiments utilising two grassland soils at two depths (topsoil and sub-soil), six ochre amendment rates (0, 0.15, 1.5, 7.5, 15 and 30 g kg−1 mass per dry weight of soil) and five P concentrations (0, 5, 10, 20 and 40 mg L−1) were carried out. A proportional equation, which incorporated P sources and losses, was developed and used to form a statistical model. Back calculation identified optimal rates of ochre amendment to soil to ameliorate a specific DRP concentration in runoff. Ochre amendment of soils (with no further P inputs) was effective at decreasing DRP concentrations to acceptable levels. A rate of 30 g ochre kg−1 soil was needed to decrease DRP concentrations to acceptable levels for P inputs of ≤10 mg L−1, which represents the vast majority of cases in grassland runoff experiments. However, although very quick and sustained metal release above environmental limits occurred, which makes it unfeasible for use as a soil amendment to control P release to a waterbody, the methodology developed within this paper may be used to test the effectiveness and feasibility of other amendments.

Previous studies have made some progress with the use of microbial community properties as assessment criteria for rehabilitation success of post-mining areas. Currently, there is a need for reference ranges of specific properties in rehabilitated post-mining sites to make this approach more practical. The aim of this investigation was to compare assessment parameters indicative of microbial community function (enzymatic assays) and structure (phospholipid fatty acid (PLFA) analysis) in rehabilitated asbestos and coal discard sites and to establish ranges of minimum and maximum values for these parameters in both types of sites. The range established for dehydrogenase activity in coal discard sites was 24.3–339.5 μg INF g−1 2 h−1 and for asbestos 44.5–544.6 μg INF g−1 2 h−1. Ranges were also established for β-glucosidase, urease, acid phosphatase and alkaline phosphatase. Complete PLFA profiles were determined and ranges established for major PLFA groups and ratios in both types of discard. From the PLFA profiles, viable microbial biomass was determined as 6,080–29,851 and 8,128–47,242 pmol g−1 dry weight for the coal and asbestos discard sites, respectively. While similar ranges were observed for both types of discard, a canonical correspondence analysis that accounts for functional and structural characteristics showed that sites clustered according to the origin of the samples.

Among the many anthropogenic abiotic stresses, manganese (Mn) toxicity has been recognized for its impact on aquatic ecosystems as well as on the biological components of these ecosystems, including aquatic plants. The objective of this study was to determine the Mn accumulation ability of aquatic macrophytes (Azolla caroliniana, Salvinia minima and Spirodela polyrhiza) and evaluate the morphophysiological responses of the species that gather the highest amount of Mn when exposed to a supra-optimal supply of manganese. The experiments were conducted in the laboratory, and the effects of Mn were evaluated based on plant growth; the concentration of total chlorophyll, carotenoids, and anthocyanins; the enzymatic activity of catalase and peroxidase; and leaf anatomy. All of the studied species accumulated Mn in their tissues. Moreover, it was observed that this accumulation was dependent on the concentration of the metal in solution. S. polyrhiza showed higher concentrations of Mn in its tissues (17.062 mg g−1 dry weight (DW)), followed by S. minima (4.283 mg g−1 DW) and A. caroliniana (1.341 mg g−1 DW). Despite the Mn accumulation in all species, S. polyrhiza was the only one selected for further analyses because of its greater ability to accumulate Mn. The high Mn concentration found in tissues of S. polyrhiza suggests that this species has the potential to sequester and accumulate this metal. However, a sensitive response in the plants exposed to higher Mn concentrations (0.4 mM) was observed. The phytotoxicity effects of this accumulation were responsible for a decrease in the plant growth, a reduction in the pigment content (total chlorophyll, carotenoids, and anthocyanins), a low activity of catalase, and the disarrangement of the leaf aerenchyma.

Olive oil mill wastewater (OMW) generates a wide variety of pollutants depending on the production process and other factors such as olive varieties and cultivation system. Efforts to mitigate the impact of these effluents in the environment have been made by developing more efficient treatment systems in terms of removal of chemical oxygen demand (COD), color, organic compounds, and toxicity. This study is the first that reports the potential of a treatment of OMW by biocomposites of silica–alginate–fungi (Pleurotus sajor caju and Trametes versicolor). The treatment by biocomposites can be considered as a three-step process responsible for the removal of the compounds: (1) adsorption of reactants on the monolithic structure and diffusion to the biological active sites, (2) biodegradation by the fungi, and (3) diffusion of the products resulting from the biodegradation. Both treatments tested showed potential capacity to remove organic compounds, color, COD, and toxicity. The T. versicolor biocomposites were the most effective and responsible for the reduction in color (from 38.4 to 44.9 %), COD (from 42.8 to 63.8 %), and total phenolic content (from 85.3 to 88.7 %) after 29 days of treatment. The toxicity reduction on Portuguese OMW was minimal, but the use of composites on the Moroccan OMW caused a 9.5- to 19-fold reduction in toxicity. Furthermore, the biocomposites showed potential for re-utilization for more 29 days of treatment.

A Cd-, Pb-, Zn-, Cu-resistant endophytic yeast CBSB78 was isolated from surface-sterilized rape roots. The isolate was identified as Cryptococcus sp. based on the ITS1–5.8S–ITS2 sequence analysis. The strain was resistant to 20 mM Cd²⁺, 20 mM Pb²⁺, 10 mM Zn²⁺, and 7 mM Cu²⁺. The yeast CBSB78 was a low indole-3-acetic acid (IAA) producer and possessed low 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Overall, 29.4–244 % of survival rates increased and the dry weight of Brassica alboglabra showed a 41.1 % increase when it was inoculated into the seedlings. The inoculation of CBSB78 could also increase the extraction amounts of Cd, Pb, and Zn by B. alboglabra simultaneously in the multi-metal contaminated soils, which showed the potential to improve extraction efficacy of Cd, Pb, Zn by B. alboglabra seedlings in the field.

In the present study, a novel synthesized adsorbent material based on 3-mercaptopropyltrimethoxysilane-functionalized multi-walled carbon nanotubes was used to increase the Pb²⁺ adsorption from aqueous solutions in a flow injection solid-phase extraction system coupled to flame atomic absorption spectrometry. Spectroscopic and microscopic techniques (Fourier transform infrared spectroscopy, energy dispersive spectroscopy, and scanning electron microscopy) were employed to confirm the chemical modification of the adsorbent surface. Preconcentration conditions (sample pH, flow rate, buffer solution, and eluent concentrations) were optimized using factorial and Doehlert matrix designs that made it possible to construct a linear graph in the 5.0- to 130.0-μg L⁻¹ range (r = 0.9999) and estimate detection and quantification limits (1.7 and 5.7 μg L⁻¹, respectively). The method precision was found to be 4.20 and 1.97 % for 5.0 and 100.0 μg L⁻¹ Pb²⁺ solutions, respectively. When using the 3-mercaptopropyltrimethoxysilane-functionalized multi-walled carbon nanotubes, the sensitivity for the Pb²⁺ trace determination was improved to 95 % compared with the oxidized multi-walled carbon nanotubes, thus evidencing the significant enhancement of the adsorption capacity. The developed method was successfully applied to the analysis of Pb²⁺ species in different water samples and the PACS-2 marine sediment-certified reference material.