Cooking and heating with solid fuels results in high levels of household air pollutants, including particulate matter (PM); however, limited data exist for size fractions smaller than PM₂.₅ (diameter less than 2.5 μm). We collected 24-h time-resolved measurements of PM₂.₅ (n = 27) and particle number concentrations (PNC, average diameter 10–700 nm) (n = 44; 24 with paired PM₂.₅ and PNC) in homes with wood-burning traditional and Justa (i.e., with an engineered combustion chamber and chimney) cookstoves in rural Honduras.The median 24-h PM₂.₅ concentration (n = 27) was 79 μg/m³ (interquartile range [IQR]: 44–174 μg/m³); traditional (n = 15): 130 μg/m³ (IQR: 48–250 μg/m³); Justa (n = 12): 66 μg/m³ (IQR: 44–97 μg/m³). The median 24-h PNC (n = 44) was 8.5 × 10⁴ particles (pt)/cm³ (IQR: 3.8 × 10⁴–1.8 × 10⁵ pt/cm³); traditional (n = 27): 1.3 × 10⁵ pt/cm³ (IQR: 3.3 × 10⁴–2.0 × 10⁵ pt/cm³); Justa (n = 17): 6.3 × 10⁴ pt/cm³ (IQR: 4.0 × 10⁴–1.2 × 10⁵ pt/cm³). The 24-h average PM₂.₅ and particle number concentrations were correlated for the full sample of cookstoves (n = 24, Spearman ρ: 0.83); correlations between PM₂.₅ and PNC were higher in traditional stove kitchens (n = 12, ρ: 0.93) than in Justa stove kitchens (n = 12, ρ: 0.67). The 24-h average concentrations of PM₂.₅ and PNC were also correlated with the maximum average concentrations during shorter-term averaging windows of one-, five-, 15-, and 60-min, respectively (Spearman ρ: PM₂.₅ [0.65, 0.85, 0.82, 0.71], PNC [0.74, 0.86, 0.88, 0.86]).Given the moderate correlations observed between 24-h PM₂.₅ and PNC and between 24-h and the shorter-term averaging windows within size fractions, investigators may need to consider cost-effectiveness and information gained by measuring both size fractions for the study objective. Further evaluations of other stove and fuel combinations are needed.

Rivers can receive the input of treated or untreated sewage effluents from wastewater treatment plants, urban and industrial discharges and agricultural run-off, becoming an important pathway for the transport and mobilization of pollutants to the oceans. In the present study, the occurrence of 20 PFAAs was determined in the water of Tagus River basin (Spain). PFAAs were detected in 76 out of 92 water samples collected during 5 years (2013–2018), being perfluorooctanesulfonic acid (PFOS) the predominant compound (<0.01–34 ng/L). The annual average PFOS concentrations (2.9–11 ng/L) detected in Tagus River were above the annual average environmental quality standards (AA-EQS) established in the Directive, 2013/39/EU (0.65 ng/L for inland surface waters) but below the maximum allowable concentration (MAC-EQS; 36000 ng/L). The levels of PFAAs detected in urban and industrial areas were statistically higher (p < 0.01) than those at background or remote areas. The mass flow rates amounted to <0.01–46 kg/y for PFOS and <0.01–22 kg/y for perfluorooctanoic acid (PFOA). A quantitative ecotoxicological risk assessment was conducted to evaluate the environmental potential risk related to PFAAs in the aquatic ecosystem. Risk characterization ratios (RCRwater, RCRsed and RCRoral, fish) were below 1 in all cases.

The stability of community functioning in anaerobic ammonia oxidation (anammox) sludge adaptation to various salinity changes are concerned but not fully explored. In this study, two anammox reactors were designed in response to different salt levels and salt-adding methods. The reactor PI, run with small stepwise salt increments (0.5%–1.0%), removed >90% of nitrite and ammonium in the influent over the range of 0%–4% salt. By contrast, the reactor SI, run with a sharp salt increment (>2.5%), exhibited a reduced performance (by up to 44%) over the same salt range with a new steady state. The observed resilience times after salt perturbations indicated that the PI reactor recovered substantially and rapidly at all imposed salt levels. Principal coordinates analysis of 16S rRNA gene amplicon sequences revealed that bacterial community structures of the anammox sludge altered conspicuously in response to the salinity changes. However, quantitative PCR analysis showed that the shift in copy number of studied nitrogen-converting genes encoding hydrazine synthase (hzsA), bacterial and archaeal ammonia monooxygenases (amoA), nitrite oxidoreductase (nxrB), nitrite reductase (nirK), and nitrous oxide reductase (nosZ) was not significant (p > 0.05) in anammox sludge across the salt levels of 0.5%–4%, which suggests the stability of microbial community functioning in the osmoadaptation processes. The freshwater anammox Ca. Kuenenia showed high osmoadaptation by potentially adopting both high-salt-in and low-salt-in strategies to dominate in both reactors. The quantitative transcript analysis showed that the active anammox bacteria represented by hzsA transcripts in the SI reactor were approximately two orders of magnitude lower than those in the PI reactor during the long-term exposure to 4% salinity, manifesting the influence by the salt-increasing methods. These results provided new insight into osmo-adaptation of the anammox microbiome and will be useful for managing salinity effects on nitrogen removal processes.

Pollution of coastal and marine environments by mismanaged anthropogenic debris is a global threat requiring complex, multilateral solutions and mitigation strategies. International efforts to catalogue and quantify the density, extent and nature of mismanaged waste have not yet assessed the heterogeneity of debris between nearby areas. Better understanding of how debris types and density can be used as a proxy between regions and between land and seafloor habitats at a global scale can aid in developing cost effective and representative debris monitoring systems. Using volunteer collected clean-up and survey data, we compared the proportion and density of both total debris and specific items across 19,428 coastal land and seafloor sites from International Coastal Cleanups and Dive Against Debris surveys, from 86 countries between 2011 and 2018. We show that although some items common on land are also common on the seafloor, there is an overall global mismatch between debris types and densities on land and the seafloor from nearby areas. Correlations in land/seafloor debris type/density occurred primarily for items which entangle and/or sink, including fishing line, plastic bags, glass and polyethylene terephthalate (PET) bottles. Minimal similarity between land and seafloor surveys occurs for items which float or degrade. We suggest that to accurately evaluate local debris density, land and seafloor surveys are required to gain a holistic understanding. When detailed information on debris type, relative concentration, and likely source and transport are assessed, more cost effective and efficient policy interventions can be designed and implemented from local through to global scales.

Vernal pools are ephemeral wetlands that provide critical habitat to many listed species. Pesticide fate in vernal pools is poorly understood because of uncertainties in the amount of pesticide entering these ecosystems and their bioavailability throughout cycles of wet and dry periods. The Pesticide Water Calculator (PWC), a model used for the regulation of pesticides in the US, was used to predict surface water and sediment pore water pesticide concentrations in vernal pool habitats. The PWC model (version 1.59) was implemented with deterministic and probabilistic approaches and parameterized for three agricultural vernal pool watersheds located in the San Joaquin River basin in the Central Valley of California. Exposure concentrations for chlorpyrifos, diazinon and malathion were simulated. The deterministic approach used default values and professional judgment to calculate point values of estimated concentrations. In the probabilistic approach, Monte Carlo (MC) simulations were conducted across the full input parameter space with a sensitivity analysis that quantified the parameter contribution to model prediction uncertainty. Partial correlation coefficients were used as the primary sensitivity metric for analyzing model outputs. Conditioned daily sensitivity analysis indicates curve number (CN) and the universal soil loss equation (USLE) parameters as the most important environmental parameters. Therefore, exposure estimation can be improved efficiently by focusing parameterization efforts on these driving processes, and agricultural pesticide inputs in these critical habitats can be reduced by best management practices focused on runoff and sediment reductions.

Heavy metal contaminants in Mirror Peninsula, East Antarctica, have rarely been studied and the source and influencing factors are poorly understood. We sampled a grid of 189 topsoil samples from Mirror Peninsula and analyzed the concentrations of Zn, Cu, U, Cr, Ga, Pb, Hg, Se and As; we also calculated the chemical index of alteration (CIA), a proxy of weathering. The results show that the distributions of Cr, Ga, Cu, and Zn are associated with weathering; the distributions of As and Pb are related to vehicle use and unloading activities at the wharfs, respectively; and the distribution of Hg is likely associated with both anthropogenic impacts and biological activity. The contamination level of these heavy metals in Mirror Peninsula is relatively low and within the controllable range. Both weathering processes and anthropogenic impacts can cause the enrichment of heavy metals; thus reliable source apportionment is crucial in studying heavy metal enrichment and contamination.

PM2.5 exposure aggravates type 2 diabetes, in which inflammatory factors play an important role. In this study, we aimed to explore the mechanisms responsible for aggravating diabetes after PM2.5 exposure, and study the roles of inflammatory factors in insulin-resistant type 2 diabetes. Our study indicated that short-time PM2.5 exposure enhances insulin resistance in type 2 diabetic rats and significantly raises inflammatory factors, including IL-6, TNF-α, and MCP-1, in lungs. However, we found that of these inflammatory factors only IL-6 levels are elevated in blood, liver, adipose tissue, and macrophages, but not in skeletal muscle. IL-6 induced activation of the STAT3/SOCS3 pathway in liver, but not other downstream pathways including STAT1, ERK1/2, and PI3K. Both STAT3 inhibition and IL-6 neutralization effectively alleviated the disorders of glucose metabolism after PM2.5 exposure. Taken together, this suggests that the systemic increase in IL-6 may play an important role in the deterioration of the type 2 diabetes via IL-6/STAT3/SOCS3 pathway in liver after short-time exposure to PM2.5. Besides, we unexpectedly found a stronger resistance to the PM2.5 exposure-induced increase in IL-6 in skeleton muscle than those of many other tissues.

Sea-spray (or sea-salt) aerosol (SSA) formation and their subsequent atmospheric transport and deposition have been suggested to play a prominent role in the occurrence of ionizable perfluoroalkyl substances (PFAS) in the maritime Antarctica and other remote regions. However, field studies on SSA’s role as vector of transport of PFAS are lacking. Following a multiphase approach, seawater (SW), the sea-surface microlayer (SML) and SSA were sampled simultaneously at South Bay (Livingston Island, Antarctica). Average PFAS concentrations were 313 pg L⁻¹, 447 pg L⁻¹, and 0.67 pg m⁻³ in SW, the SML and SSA, respectively. The enrichment factors of PFAS in the SML and SSA ranged between 1.2 and 5, and between 522 and 4690, respectively. This amplification of concentrations in the SML is consistent with the surfactant properties of PFAS, while the large enrichment of PFAS in atmospheric SSA may be facilitated by the large surface area of SSA and the sorption of PFAS to aerosol organic matter. This is the first field work assessing the simultaneous occurrence of PFAS in SW, the SML and SSA. The large measured amplification of concentrations in marine aerosols supports the role of SSA as a relevant vector for long-range atmospheric transport of PFAS.

Microplastics (MPs) are contaminants of increasing concern due to their abundance, ubiquity and persistence over time. However, knowledge about MP distribution in fresh waters and their effects on freshwater organisms is still scarce, and there is virtually no information about their potential influence on ecosystem functioning. We used a microcosm experiment to examine the effects of MPs (fluorescent, 10-μm polystyrene microspheres) at different concentrations (from 0 to 10³ particles mL⁻¹) on leaf litter decomposition (a key process in stream ecosystems) and associated organisms (the caddisfly detritivore Sericostoma pyrenaicum), and the extent to which MPs were attached to leaf litter and ingested and egested by detritivores, thus assessing mechanisms of MP trophic transfer. We found that MPs caused detritivore mortality (which increased 9-fold at the highest concentration) but did not affect their growth. Analysis of fluorescence in samples suggested that MPs were rapidly ingested (most likely through ingestion of particles attached to leaf litter) and egested. Leaf litter decomposition was reduced as a result of increasing MP concentrations; the relationship was significant only in the presence of detritivores, but microbially-mediated decomposition showed a similar trend. Our findings provide novel evidence of harmful effects of MPs on aquatic insects and stream ecosystem functioning, and highlight the need for the standardization of methods in future experiments with MPs in order to allow comparisons and generalizations.

Tropospheric ozone (O₃) is a pollutant of widespread concern in the world and especially in China for its negative effects on agricultural crops. For the first time, yield and economic losses of wheat between 2014 and 2017 were estimated for the North China Plain (NCP) using observational hourly O₃ data from 312 monitoring stations and exposure-response functions based on AOT40 index (accumulated hourly O₃ concentration above 40 ppb) from a Chinese study. AOT40 values from 2014 to 2017 during the wheat growing seasons (75-days, 44 before and 30 after mid-anthesis) ranged from 3.1 to 14.9 ppm h, 4.9–17.5 ppm h, 7.3–17.6 ppm h, and 0.5–18.6 ppm h, respectively. The highest AOT40 values were observed in the Beijing-Tianjin-Hebei region. The values of relative yield losses from 2014 to 2017 were in the ranges of 6.4–30.5%, 10.0–35.8%, 14.9–34.1%, and 21.6–38.2%, respectively. The total wheat production losses in NCP for 2014–2017 accounted for 18.5%, 22.7%, 26.2% and 30.8% in the whole production, while the economic losses amounted to 6,292 million USD, 8,524 million USD, 10,068 million USD, and 12,404 million USD, respectively. The important impact of O₃ in this area, which is of global importance, should be considered when assessing wheat yield production. Our results also show an increasing trend in AOT40, relative yield loss, total crop production loss and economic loss in the four consecutive years.