Ozonation of water effluent polluted with carbamazepine an ubiquitous and refractory pharmaceutical contaminant, has been addressed. This paper aims to optimize the remediation process through novel considerations, such as economical aspects of operational costs. To this end, firstly, we have defined an efficiency variable which included not only global efficacy terms (pollutant removal) but also kinetic aspects, which has to do with the ozonation chemical rate. This target variable was involved in a design of experiments that optimized air flow, ozone concentration, and pollutant initial content. An optimum was obtained at 55 L·h ⁻ ¹, 0.4 g·m³, and 18 mg·L ⁻ ¹ respectively.

Stem cuttings with homogenous diameter of Populus x euramericana (clone I-214) and Salix fragilis L. were grown in growth chamber in water culture method. After 45 days, the plants were treated with 10−7 and 10−5 M cadmium (Cd). As these species have different phytoextraction potentials, there is a need to analyze the level of Cd uptake, its translocation into aboveground organs, and changes in leaf structure. We analyzed micromorphological leaf characteristics: a fresh mass of the root, stem, and leaf, as well as a Cd concentration within them. Besides, we compared 23 micromorphological leaf blade quantitative traits of poplar and willow and monitored the structural changes induced by the intoxication of stem cuttings. Percent of Cd accumulation and translocation in plant organs varied between species. It depended on the level of Cd applied. When compared to the poplar clone, S. fragilis had a smaller leaf area and epidermal cells, thicker palisade tissue, smaller lumen of main vein vessels, and a higher percentage of main vein xylem. S. fragilis had more distinctive xeromorphic characteristics in the lamina structure. Increased concentrations of Cd led to significant structural changes, mainly in the main vein. When searching for valid parameters in assessing plant to be utilized in phytoremediation, it is necessary to take into consideration the interrelation of a large number of micromorphological parameters together with physiological and biochemical characteristics.

Single and joint ectomycorrhizal (+ Hebeloma mesophaeum) and bacterial (+ Bacillus cereus) inoculations of willows (Salix viminalis) were investigated for their potential and mode of action in the promotion of cadmium (Cd) and zinc (Zn) phytoextraction. Dual fungal and bacterial inoculations promoted the biomass production of willows in contaminated soil. Single inoculations either had no effect on the plant growth or inhibited it. All inoculated willows showed increased concentrations of nutritional elements (N, P, K and Zn) and decreased concentrations of Cd in the shoots. The lowest biomass production and concentration of Cd in the willows (+ B. cereus) were combined with the strongest expression of metallothioneins. It seems that biotic stress from bacterial invasion increased the synthesis of these stress proteins, which responded in decreased Cd concentrations. Contents of Cd and Zn in the stems of willows were combination-specific, but were always increased in dual inoculated plants. In conclusion, single inoculations with former mycorrhiza-associated B. cereus strains decreased the phytoextraction efficiency of willows by causing biotic stress. However, their joint inoculation with an ectomycorrhizal fungus is a very promising method for promoting the phytoextraction of Cd and Zn through combined physiological effects on the plant.

Air pollutants emissions from traffic are very closely connected to urban air quality, in a local scale, as well as to global problems like climate change, in a large scale. Road transport air pollutants emissions represent, in most cases, a critical parameter for a comprehensive and successful understanding of the mechanisms governing the air pollutants concentrations. Hence, reliable estimations and comprehension of road transport emissions are indispensable in order to set reliable strategies in the direction of air pollution abatement and management of air pollutants and greenhouse gases emissions. In this framework, in the present work, the emissions of air pollutants from road transport in Greece will be presented for the whole period 1990–2009 as it was found that a detailed, accurate and reliable emissions inventory was missing. The whole period emissions variation has clarified the impact of the change in the vehicle fleet, the engine technologies and the fuel quality. The calculated results have revealed that the age of the vehicles and the corresponding engine technology are the critical parameters determining the amount of the pollutants emitted. This was mainly observed in both passenger cars and heavy duty vehicles demonstrating the importance of a renewal programme of the old circulating vehicles in order to set an effective air pollution abatement strategy. Passenger cars were found to be responsible for the major part of most air pollutants emissions except from nitrogen oxides and particulates emissions. Heavy duty vehicles contribute more than 66% to nitrogen oxides and particulates emissions. For the whole time period, all calculated pollutants present a decreasing trend, with the exception of carbon dioxide and nitrous oxide which increase constantly, ranging from −96% for sulphur dioxide to −1% for PM10.

The efficiency of the photocatalytic degradation of the herbicide quinmerac in aqueous TiO2 suspensions was examined as a function of the type of light source, TiO2 loading, pH, temperature, electron acceptors, and hydroxyl radical (.OH) scavenger. The optimum loading of catalyst was found to be 0.25 mg mL−1 under UV light at pH 7.2, with the apparent activation energy of the reaction being 13.7 kJ mol−1. In the first stage of the reaction, the photocatalytic degradation of quinmerac (50 μM) followed approximately a pseudo-first order kinetics. The most efficient electron acceptor appeared to be H2O2 along with molecular oxygen. By studying the effect of ethanol as an .OH scavenger, it was shown that the heterogeneous catalysis takes place mainly via .OH. The results also showed that the disappearance of quinmerac led to the formation of a number of organic intermediates and ionic byproducts, whereas its complete mineralization occurred in about 120 min. The reaction intermediates (7-chloro-3-methylquinoline-5,8-dione, three isomeric phenols hydroxy-7-chloro-3-methylquinoline-8-carboxylic acids, and 7-chloro-3-(hydroxymethyl)quinoline-8-carboxylic acid) were identified and the kinetics of their appearance/disappearance was followed by LC–ESI–MS/MS. Tentative photodegradation pathways were proposed and discussed. The study also encompassed the effect of quality of natural water on the rate of removal of quinmerac.

In order to enhance the removal of heavy metals such as Ni, Cu, Zn and Cd from wastewater, different cow dung/sewage sludge ratios were tested to assess the effect of these metals on the adaptability of Eisenia fetida earthworms to the treatment process carried out in a typical plant located in Tamaulipas, Mexico. Two experimental water treatment setups were proposed. The first set of experiments was planned to determine the adequate sewage sludge/cow dung ratio(s), whereas the second arrangement was designed to evaluate the growth performance and fecundity of the earthworms under high heavy metal concentrations. To achieve the objectives, the experiments were conducted for 90 days under controlled environmental conditions. Maximum worm biomass and growth rates were attained in samples containing 25 wt.% of sewage sludge. Weight and mortality of worms were significantly affected by the high levels of heavy metals, making difficult the metal accumulation in the worm tissues.

Surface sediments (0–5 cm) were analysed to provide information on levels, spatial trends and sources of the 16 USEPA polycyclic aromatic hydrocarbons (PAH), 15 polychlorinated biphenyls (PCBs) and trace metals (copper, chromium, mercury, nickel and zinc) in channel and wetland habitats of Pialassa Baiona lagoon (Italy). The highest levels of PAHs, PCBs and Hg (3,032–87,150, n.d.–3,908 and 1.3–191 mg kg−1) were mainly found at channel habitats close to industrial sources. Pyrogenic PAH inputs were significant, with a predominance of four-ring PAHs and combustion-related PAHs in both channel and wetland habitats. Among PCB congeners, chlorination class profiles show that penta- and hexachlorinated PCBs are the most prevalent homologues accounting for approximately 33% and 47% of the total PCB concentrations in channel sediments. Total toxicity equivalent factors (TEQs) of potentially carcinogenic PAHs varied from 348 to 7,879 μg kg−1 and from 4.3 to 235 μg kg−1 in channel and wetland sediments; calculated TEQs for dioxin-like PCB congeners at channel habitats ranged from n.d. to 86.7 μg kg−1. Comparison of PAHs, PCBs and metal levels with Sediment Quality Guidelines suggests that more concern should be given to the southern area of the lagoon for potential risks of carcinogenic PAHs, dioxin-like PCBs and mercury.

The batch bioreduction of Cr(VI) by the cells of newly isolated chromium-resistant Acinetobacter sp. bacteria, immobilized on glass beads and Ca-alginate beads, was investigated. The rate of reduction and percentage reduction of Cr(VI) decrease with the increase in initial Cr(VI) concentration, indicating the inhibitory effect of Cr(VI). Efficiency of bioreduction can be improved by increasing the bioparticle loading or the initial biomass loading. Glass bioparticles have shown better performance as compared to Ca-alginate bioparticles in terms of batch Cr(VI) reduction achieved and the rate of reduction. Glass beads may be considered as better cell carrier particles for immobilization as compared to Ca-alginate beads. Around 90% reduction of 80 ppm Cr(VI) could be achieved after 24 h with initial biomass loading of 14.6 mg on glass beads. Artificial neural network-based models are developed for prediction of batch Cr(VI) bioreduction using the cells immobilized on glass and Ca-alginate beads.

Slaughterhouse waste water (SHW) is discarded as unused disposals into the environment every day. The objective of the present study is to explore the possibility on the utilization of SHW for the preparation of culture media to produce mosquitocidal bacterium (Bacillus thuringiensis subsp. israelensis). This would help to overcome the problem of dumping SHW in to environment. A judicial combination of SHW with a mineral salt (MnCl2) was made to produce an enhanced level of bacterial production when compared with other culture media including conventional medium (Luria Bertani, LB). A complete degradation of SHW by the bacteria was observed. The biomass yield, bacterial growth, toxin production, and larvicidal activity against mosquito vectors were satisfactory. Cell mass yield of 4.55 gm l−1 (dry wt) and larvicidal activity of 0.006 mg ml−1 and 0.026 mg ml−1 at LC50 and LC90 levels were observed, respectively, against the filarial vector of Culex quinquefasciatus with bacteria grown in SHW + MnCl2. The B. thuringiensis subsp. israelensis also controlled the larvae in the field significantly for three weeks (>90% mortality) and the effect was comparable with LB. Cost-analysis for production of B. thuringiensis subsp. israelensis showed that it is more economical. Thus, this study suggested the dual benefit of efficient production of mosquitocidal toxin and management of slaughterhouse wastewater.

Clogging is the main operational problem associated with horizontal subsurface flow constructed wetlands (HSSF CWs). The measurement of saturated hydraulic conductivity has proven to be a suitable technique to assess clogging within HSSF CWs. The vertical and horizontal distribution of hydraulic conductivity was assessed in two full-scale HSSF CWs by using two different in situ permeameter methods (falling head (FH) and constant head (CH) methods). Horizontal hydraulic conductivity profiles showed that both methods are correlated by a power function (FH = CH0.7821, r 2 = 0.76) within the recorded range of hydraulic conductivities (0–70 m/day). However, the FH method provided lower values of hydraulic conductivity than the CH method (one to three times lower). Despite discrepancies between the magnitudes of reported readings, the relative distribution of clogging obtained via both methods was similar. Therefore, both methods are useful when exploring the general distribution of clogging and, specially, the assessment of clogged areas originated from preferential flow paths within full-scale HSSF CWs. Discrepancy between methods (either in magnitude and pattern) aroused from the vertical hydraulic conductivity profiles under highly clogged conditions. It is believed this can be attributed to procedural differences between the methods, such as the method of permeameter insertion (twisting versus hammering). Results from both methods suggest that clogging develops along the shortest distance between water input and output. Results also evidence that the design and maintenance of inlet distributors and outlet collectors appear to have a great influence on the pattern of clogging, and hence the asset lifetime of HSSF CWs.