An in-depth understanding of the impacts of surface roughness on road-deposited sediment (RDS) build-up and wash-off is essential for the estimation of surface runoff loads and design of RDS control measures. In this study, RDS build-up and wash-off dynamic processes were investigated on paired asphalt and concrete road surfaces with 35 days of continuous sampling during different natural rainfall events. Our results showed that RDS build-up loads and grain size composition were affected by surface roughness, while the impact of surface roughness on the length of the dynamic equilibrium period was not notable. Selective wash-off of RDS with different effects according to grain size are more likely to occur on asphalt road surfaces during rainfall-runoff, but the RDS wash-off percentage is not affected by surface roughness during snowmelt-runoff. Both total apparent depression depth and micro-depression structures influence RDS build-up and wash-off dynamics. These results imply that surface roughness has combined effects on RDS build-up and wash-off dynamics during the generation and control of urban diffuse pollution.

Nitrous oxide (N₂O) is a major greenhouse gas, with elevated emission being reported from subtropical forests that receive high nitrogen (N) deposition. After 10 years of monthly addition of ammonium nitrate (NH₄NO₃) or sodium nitrate (NaNO₃) to a Mason pine forest at Tieshanping, near Chongqing city in Southwest China, the simulated N deposition was stopped in October 2014. The results of soil N₂O emissions monitoring in different seasons during the nitrogen application period showed that nitrogen addition significantly increased soil N₂O emission. In general, the N₂O emission fluxes were positively correlated to nitrate (NO₃⁻) concentrations in soil solution, supporting the important role of denitrification in N₂O production, which was also modified by environmental factors such as soil temperature and moisture. After stopping the application of nitrogen, the soil N₂O emissions from the treatment plots were no longer significantly higher than those from the reference plots, implying that a decrease in nitrogen deposition in the future would cause a decrease in N₂O emission. Although the major forms of N deposition, NH₄⁺ and NO₃⁻, had not shown significantly different effects on soil N₂O emission, the reduction in NH₄⁺ deposition may decrease the NO₃⁻ concentrations in soil solution faster than the reduction in NO₃⁻ deposition, and thus be more effective in reducing N₂O emission from N-saturated forest soil in the future.

Several studies have been carried out to examine nitrous oxide (N₂O) emissions from agricultural soils in the past. However, the emissions of N₂O particularly during amelioration of acidic soils have been rarely studied. We carried out the present study using a rice-rapeseed rotation soil (pH 5.44) that was amended with dolomite (0, 1 and 2 g kg⁻¹ soil) under 60% water filled pore space (WFPS) and flooding. N₂O emissions and several soil properties (pH, NH₄⁺N, NO₃⁻-N, and nosZ gene transcripts) were measured throughout the study. The increase in soil pH with dolomite application triggered soil N transformation and transcripts of nosZ gene controlling N₂O emissions under both water regimes (60% WFPS and flooding). The 60% WFPS produced higher soil N₂O emissions than that of flooding, and dolomite largely reduced N₂O emissions at higher pH under both water regimes through enhanced transcription of nosZ gene. The results suggest that ameliorating soil acidity with dolomite can substantially mitigate N₂O emissions through promoting nosZ gene transcription.

Recent decade has witnessed accelerating expansion of chemical industry and increasing conflicts between the local citizens, governmental authorities and project developers, especially in some coastal and port cities in China. Development and transformation of chemical industrial parks has been adopted as a national initiative recently. However, there is a paucity of research examining public perspectives on chemical industrial parks and their risks. Aiming to understand public perception, attitude, and response and the factors underlying the support/acceptance of chemical industry park, this paper investigated 418 residents neighboring to two chemical industrial parks, Dalian in Bohai Rim through face-to-face questionnaire survey. The results showed the knowledge of the respondents on the chemical industrial parks development was very limited. The respondents had complex perceptions on the environmental impacts, risks control, social-economic benefits, and problem awareness. The current levels of information disclosure and public participation were very low. The central governmental official (44.3%) was the most trustworthy group by the respondents. Only 5.5% and 23.2% of the respondents supported the construction of a new CIP nearby and far away their homes, whilst 13% thought new CIP project as acceptable. The spearman correlation analysis results showed a strong NIMBY effect (Not In My Backyard). Factor analysis results demonstrated five latent factors: knowledge, benefit, information, trust, and participation. Multiple linear regression analysis indicated how socio-demographic differences and five latent factors might impact on the support/acceptance of the chemical industrial parks. Education level, trust, information, and participation were significant predictors of public support/acceptance level. This study contributes to our limited knowledge and understanding of public sentiments to the chemical industry parks in China.

Bis(2-ethylhexyl) phthalate (DEHP) is the most common plasticizer. Previous studies have shown DEHP treatment accelerates neurological degeneration, suggesting that DEHP may impact retinal sensitivity to light, neurotransmission, and copulation behaviors. Although its neurotoxicity and antifertility properties have been studied, whether DEHP exposure disrupts vision and how DEHP influences neuromuscular junction (NMJ) have not been reported yet. Moreover, the impact of DEHP on insect courtship behavior is still elusive. Fruit flies (Drosophila melanogaster) were treated with series concentrations of DEHP and observed for lifespan, motor function, electroretinogram (ERG), electrophysiology of neuromuscular junction (NMJ), courtship behaviors, and relevant gene expression. Our results confirmed the DEHP toxicity on lifespan and capacity of motor function and updated its effect on copulation behaviors. Additionally, we report for the first time that DEHP exposure may harm vision by affecting the synaptic signaling between the photoreceptor and the laminar neurons. Further, DEHP treatment altered both spontaneous and evoked neurotransmission properties. Noteworthy, the effect of DEHP exposure on the copulation behavior is sex-dependent, and we proposed potential mechanisms for future investigation.

Multi-stage constructed wetlands (CWs) have been proved to be a cost-effective alternative in the treatment of various wastewaters for improving the treatment performance as compared with the conventional single-stage CWs. However, few long-term full-scale multi-stage CWs have been performed and evaluated for polishing effluents from domestic wastewater treatment plants (WWTP). This study investigated the seasonal and spatial dynamics of carbon and the effects of the key factors (input loading and temperature) in the large-scale seven-stage Wu River CW polishing domestic WWTP effluents in northern China. The results indicated a significant improvement in water quality. Significant seasonal and spatial variations of organics removal were observed in the Wu River CW with a higher COD removal efficiency of 64–66% in summer and fall. Obvious seasonal and spatial variations of CH₄ and CO₂ emissions were also found with the average CH₄ and CO₂ emission rates of 3.78–35.54 mg m⁻² d⁻¹ and 610.78–8992.71 mg m⁻² d⁻¹, respectively, while the higher CH₄ and CO₂ emission flux was obtained in spring and summer. Seasonal air temperatures and inflow COD loading rates significantly affected organics removal and CH₄ emission, but they appeared to have a weak influence on CO₂ emission. Overall, this study suggested that large-scale Wu River CW might be a potential source of GHG, but considering the sustainability of the multi-stage CW, the inflow COD loading rate of 1.8–2.0 g m⁻² d⁻¹ and temperature of 15–20 °C may be the suitable condition for achieving the higher organics removal efficiency and lower greenhouse gases (GHG) emission in polishing the domestic WWTP effluent. The obtained knowledge of the carbon dynamics in large-scale Wu River CW will be helpful for understanding the carbon cycles, but also can provide useful field experience for the design, operation and management of multi-stage CW treatments.

Microcystin-leucine-arginine (MC-LR), as a most common and deleterious variant among all structural analogues of Microcystins (MCs), can cause male reproductive dysfunction. However, its toxic effects on prostate in adult mice have not been invested in detail. In this study, we observed that MC-LR could enter prostate tissues and induce focal hyperplasia and prostate inflammation. Moreover, increased levels of prostate specific antigen (PSA) and prostate acid phosphatase (PAP) in serum of mice following chronic exposure to MC-LR were detected. We also examined increased expression of forkhead box protein M1 (FOXM1) and PSA in human prostate epithelial cells (RWPE-1) treated with MC-LR at low levels, and FOXM1 could regulate PSA expression. Furthermore, MC-LR also induced expression of CyclinD1 via FOXM1/Wnt/β-catenin signaling pathways in RWPE-1 cells, promoting proliferation of prostate epithelial cells, resulting in prostatic hyperplasia in vivo. As a foreign substance, MC-LR also induced immune reaction in RWPE-1 cells mediated by NF-κB pathway, promoting production of pro-inflammatory cytokines and chemokines. Collectively, these findings demonstrated that MC-LR may induce prostatic hyperplasia and prostatitis in mice following chronic low-dose exposure to MC-LR. This work may provide new perspectives in developing new diagnosis and treatment strategies for MC-LR-induced prostatic toxicity.

As a pristine continent, Antarctica provides a good opportunity to study the spatial transport and temporal accumulation of environmental contaminants and the impacts of anthropogenic activities, both of which have given rise to ongoing public concern. In this research, an approach of coupling aquatic time-integrated passive sampling with chemical analysis and bioassays was used to assess pollution by hydrophobic organic contaminants in Antarctic waters. Passive samplers were deployed in waters of Fildes Peninsula, Antarctica, and their extracts were used for chemical analyses of sixty-six hydrophobic organic contaminants belonging to five groups [organophosphorus flame retardants (PFRs), phthalic acid esters (PAEs), polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), and polychlorinated biphenyls (PCBs)] and in vitro bioassays for endocrine disruption and genotoxicity. In total, twenty pollutants (six PFRs, one PAE, two PAHs, six OCPs, and five PCBs) were quantified, and six PFRs had concentrations that ranged from ND (not detected) to 44.37 ng L−1 in Antarctic waters. The concentrations detected in the waters were generally low and insufficient to have significant in vitro endocrine disruption potential or genotoxicity. The source and transport pathways of PFRs and PAE in Fildes Peninsula were studied, and multiple local sources (wastewater, air traffic, research stations, and animal feces) for different PFRs were proposed. A spatial and temporal analysis showed slight changes in the exposure of OCPs and PCBs in Antarctic waters. Furthermore, a comparison among a variety of Antarctic water sampling cases revealed that passive sampling can be a tool for aquatic time-integrated investigations in polar regions.

Water diversion has been increasingly applied to improve water quality in many water bodies. However, little is known regarding pollution by organic micropollutants (OMPs) in water diversion projects, especially at the supplier, and this pollution may threaten the quality of transferred water. In the present study, a total of 110 OMPs belonging to seven classes were investigated in water and sediment collected from a supplier of the Yangtze River within four water diversion projects. A total of 69 and 58 target OMPs were detected in water and sediment, respectively, at total concentrations reaching 1041.78 ng/L and 5942.24 ng/g dry weight (dw). Polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals were the predominant pollutants identified. When preliminarily compared with the pollution in the receiving water, the Yangtze River generally exhibited mild OMPs pollution and good water quality parameters, implying a clean water source in the water diversion project. However, in Zongyang and Fenghuangjing, PAHs pollution was more abundant than that in the corresponding receiving water in Chaohu Lake. Ammonia nitrogen pollution in the Wangyu River was comparable to that in Taihu Lake. These findings imply that water diversion may threaten receiving waters in some cases. In addition, the risks of all detected pollutants in both water and sediment were assessed. PAHs in water, especially phenanthrene and high-molecular-weight PAHs, posed high risks to invertebrates, followed by the risks to fish and algae. Pharmaceuticals, such as antibiotics and antidepressants, may also pose risks to algae and fish at a number of locations. To the best of our knowledge, this report is the first to describe OMPs pollution in water diversion projects, and the results provide a new perspective regarding the security of water diversion projects.

Nitrite is a common pollutant in water and is highly toxic to aquatic animals. To reveal the mechanism of salinity in attenuating nitrite toxicity to fish, we measured the physiological responses of juvenile Takifugu obscurus exposed to nitrite concentrations (0, 10, 20, 50, and 100 mg/L) under different salinity levels (0, 10, and 20 ppt) for 96 h. Salinity increased the survival rates of juvenile T. obscurus exposed to nitrite. Changes in key hematological parameters, antioxidant system, malondialdehyde, Na⁺/K⁺–ATPase, and HSP70 indicated that nitrite induced considerable damage to juveniles; salinity mitigated the harmful effects. This finding reflects similar changing trends in both antioxidants and their gene expressions among different tissues. We applied an overall index, an integrated biomarker response (IBR), that increased under high−nitrite condition but recovered to the normal levels under salinity treatment. Analysis of the selected detection indices and IBR values showed that the overall mitigating effect of salinity on nitrite toxicity seems to be at sub-cellular level and associated with complicated physiological responses.