The neonicotinoid insecticide acetamiprid is widely applied for pest control in agriculture production, and its exposure often results in adverse effects on a non-target insect, Bombyx mori. However, only few studies have investigated the effects of exposure to sublethal doses of neonicotinoid insecticides on gut microbiota and susceptibility to pathogenic bacteria. In this study, we aimed to explore the possible mechanisms underlying the acetamiprid-induced compositional changes in gut microbiota of silkworm and reduced host resistance against detrimental microbes. This study indicated that sublethal dose of acetamiprid activated the dual oxidase-reactive oxygen species (Duox-ROS) system and induced ROS accumulation, leading to dysregulation of intestinal immune signaling pathways. The evenness and structure of bacterial community were altered. Moreover, after 96 h of exposure to sublethal dose of acetamiprid, several bacteria, such as Pseudomonas sp (Biotype A, DOP-1a, XW34) and Staphylococcus sp (RCB1054, RCB314, X302), invaded the silkworm hemolymph. The survival rate and bodyweight of the acetamiprid treated silkworm larvae inoculated with Enterobacter cloacae (E. cloacae) were significantly lower than the acetamiprid treatment group, suggesting that acetamiprid reduced silkworm resistance against pathogens. These findings indicated that acetamiprid disturbed gut microbial homeostasis of Bombyx mori, resulting in changes in gut microbial community and susceptibility to detrimental microbes.

This work aimed to study the performance of paraquat removal by cell-immobilized ceramics. Two strains of paraquat degrading bacteria, Pseudomonas putida and Bacillus subtilis, were separately immobilized on the ceramic with and without wastewater sludge addition. Results showed that the ceramic surface with sludge has more functional groups and a more highly negative charge on the surface than the original ceramic. The ceramic with sludge had 2-3-fold of the immobilized cells higher than that of the control (without sludge) and less leaching of the immobilized cells. The sludge addition at 20% (w/w) to the ceramic provided the highest cell adhesion for both P. putida and B. subtilis. The paraquat removal efficiencies were higher than 98%, while the control ceramic could remove only 77 ± 1.2%. The immobilized cells on ceramic with sludge provided a significant degree of dissolved organic nitrogen reduction (82%) during the paraquat removal. Most organic nitrogen in paraquat was biologically mineralized (ammonified). Findings from this work suggest the superiority of ceramic with sludge in mineralizing organic nitrogen associated with paraquat.

Due to their small dimensions, airborne particles are able to penetrate through inhalation into many human organs, from the lungs to the cardiovascular system and the brain, which can threaten our health. This work establishes a novel approach of collecting quantitative data regarding the fraction, the composition and the size distribution of combustion-emitted particulate matter through the magnetic characterization and analysis of samples received by common air pollution monitoring. To this end, SQUID magnetometry measurements were carried out for samples from urban and suburban areas in Thessaloniki, the second largest city of Greece, taking into consideration the seasonal and weekly variation of airborne particles levels as determined by occurring traffic and meteorological conditions. The level of estimated magnetically-responding atmospheric particulate matter was at least 0.5 % wt. of the collected samples, mostly being present in the form of ultrafine particles with nuclei sizes of approximately 14 nm and their aggregates. The estimated quantities of magnetic particulate matter show maximum values during autumn months (0.8 % wt.) when increased commuting takes place, appearing higher in the city center by up to 50% than those in suburban areas. In combination with high-resolution transmission electron imaging and elemental analysis, it was found that Fe₃O₄ and similar ferrites, some of them attached to heavy metals (Co, Cr), are the dominant magnetic contributors arising from anthropogenic high-temperature processes, e.g. due to traffic emissions. Importantly, nasal cytologic samples collected from residents of both central and suburban areas showed same pattern in what concerns magnetic behavior, thus verifying the critical role of nanosized magnetic particles in the assessment of air pollution threats. Despite the inherent statistical limitations of our study, such findings also indicate the potential transmission of infectious pathogens by means of pollution-derived nanoparticles into the respiratory system of the human body.

Although crop residue return increases upland soil emissions of nitrous oxide (N₂O), a potent greenhouse gas, the mechanisms responsible for the increase remain unclear. Here, we investigate N₂O emission pathways, gross nitrogen (N)-cycling rates, and associated N-cycling gene abundances in an upland soil following the addition of various organic material under aerobic incubation using a combination of ¹⁵N tracing technique, acetylene (C₂H₂) inhibition, and real-time PCR (qPCR) methods. Increased total N₂O emissions following organic material amendment was attributed to both increased nitrification-derived N₂O emissions, following increased ammonia-oxidizing bacteria (AOB)-amoA abundance, and denitrification-derived N₂O emissions, following increased nirS and decreased nosZ abundance. Increasing plant residue carbon (C)/N ratio decreased total N₂O emissions by decreasing the contribution of denitrification to N₂O emissions, potentially due to higher proportions of denitrified N emitted as N₂O than nitrified N emitted as N₂O. We further propose a novel conceptual framework for organic material input effects on denitrification-derived N₂O emissions based on the decomposable characteristics of the added organic material. For slowly decomposing organic materials (e.g., plant residue) with insufficient available C, NO₃⁻-N immobilization surpassed denitrification, resulting in gradual decrease in denitrification-derived N₂O emissions with an increase in mineralization of plant residue C losses. In contrast, available C provided by readily available C sources (e.g., glucose) seemed sufficient to support the co-occurrence of NO₃⁻-N immobilization and denitrification. Overall, for the first time, we offer a microbial process perspective of N₂O emissions following organic material input. The findings could facilitate the improvement of process-orientated models of N₂O emissions and the formulation of appropriate N₂O mitigation strategies for crop residue-amended soils.

Microplastic (MP) contamination in soil has attracted much attention, and increasing evidence suggests that MPs can accumulate in agricultural soils through fertilization by compost. In addition, the most common raw materials for composting are livestock and poultry manure wastes. Because the presence of MPs may threaten the safe utilization of fertilizers composted by livestock and poultry wastes during crop planting, it is necessary to understand the contamination risk of MPs present in livestock and poultry manure. In this study, the distribution of MPs in 19 livestock and poultry farms with 3 different species was investigated by using FTIR microscopy. A total of 115 items manure MPs and 18 items feed MPs were identified as PP and PE types dominated by colorful fragments and fibers. Furthermore, after comparing the compositions of plastic products used in the feeding process, we proposed two transport pathways for MP pollution in manure and one potential transport pathway in feeds. Our result proved that the application of swine and poultry manure directly could be a new route of MPs in agricultural soil, furthermore, the presence of MPs could threaten the safety of resource utilization in agricultural soil by using swine and poultry manure for manure potentially. Not, only that, our study also provided a reference for the remediation of MP-contaminated soil.

Microplastic pollution in the Yangtze River Basin has become a major concern; however, the variations in different environmental compartments are unknown. Here, we compiled published information including detection methods, occurrence, and characterization of microplastics from 624 sampling sites in river water, river sediment, lake and reservoir water, and lake and reservoir sediment in the Yangtze River Basin. The spatial distribution of sampling sites shows fractal pattern and was uniformly concentrated around the main stream of the Yangtze River and the lake geographical zone. Collection, pretreatment, identification, and quantification processes varied among different studies. Non-parametric tests were performed to compare the different microplastic indices. A Pearson correlation analysis was used to study the relationship between microplastic pollution and local socioeconomic conditions. We found that the microplastic size and abundance distribution in river water and lake and reservoir water showed different patterns for different sampling methods, indicating that different methods influenced the results. Population density and urbanization rate are suggested to be important factors influencing the spatial heterogeneity of microplastic abundances in water, rather than in sediment. The microplastic abundances in lake and reservoir water were higher than that in river water in bulk samples. However, microplastic abundances among different sediment environments shows no significant difference. For bulk water samples and sediment samples overall, the proportion of small microplastics (<1 mm, i.e. SMP), fibers, transparent debris, and polypropylene (PP) were 65.1%, 67.8%, 31.8%, and 29.7%, respectively. The microplastic characteristics of lake and reservoir water and sediment were similar, differing from those of river water and sediment. This study provides the first basin scale insight into microplastic occurrence and characteristics in different environments in the Yangtze River Basin.

China is experiencing severe tropospheric ozone pollution, especially during the summer period in cities. Previous studies have assessed the role of meteorological conditions and anthropogenic precursors in shaping the diurnal variation of ozone concentration in some Chinese cities or the spatial patterns of daytime ozone concentration, but less is known about the spatial variation and main regulators of the diurnal cycle of summer ozone concentrations in Chinese cities. Using monitoring data from 367 cities, we analyzed the spatial patterns and main regulators of daytime maximum, nighttime minimum and diurnal difference of summer (June–August) ozone concentration during 2015–2019. National mean values and standard deviations of daytime maximum and nighttime minimum of summer surface ozone concentration were 124.1 ± 27.5 and 33.4 ± 13.0 μg m⁻³, resulting in a diurnal difference of 90.7 ± 25.2 μg m⁻³. High values of daytime maximum, nighttime minimum, and diurnal difference of summer ozone concentration occurred in cities in northern China, especially in the North China Plain, and several city agglomerations in southern China. Daytime maximum ozone concentration was higher in cities with higher daytime PM₂.₅ and NO₂ concentrations, lower daytime precipitation and lower elevation. Nighttime minimum ozone concentration increased with lower nighttime precipitation, lower NO₂ concentration and CO concentration, higher nighttime maximum PM₂.₅ concentration and higher elevation. Diurnal difference of ozone concentration increased with lower elevation, lower daytime precipitation, and higher diurnal difference of CO and NO₂ concentrations. Our findings highlight different regulators for daytime and nighttime ozone and imply the need of joint regulation of PM₂.₅ and NO₂ emissions to control ozone pollution.

Urban green infrastructure is closely linked to the alleviation of pollution from atmospheric particulate matter. Although particle deposition has been shown to depend on leaf characteristics, the findings from earlier studies are sometimes ambiguous due to the lack of controlling variables. In this study, we investigated the impact of leaf morphological characteristics on PM₂.₅ dry deposition velocity by employing a control-variable approach. We focused on four indices: trichome density, petiole length, aspect ratio (width-to-length ratio), and fractal deviation. For each index, tree species were chosen from the same family or genus to minimize the influence of other factors and make a group of treatments for an individual index. The dry deposition velocities of PM₂.₅ were determined through application of an indirect method. The results revealed that the presence of leaf trichomes had a positive effect on PM₂.₅ dry deposition velocity, and a higher trichome density also led to a greater particle deposition velocity. Lower leaf aspect ratio, shorter petioles, and higher leaf fractal deviation were associated with greater PM₂.₅ dry deposition velocity. The control-variable approach allows to investigate the correlation between deposition velocity and a certain leaf characteristic independently while minimizing the effects of others. Thus, our study can clarify how a single leaf characteristic affects particle deposition velocity, and expound its potential mechanism more scientifically than the published studies. Our research points out the importance of controlling variables, and also provides ideas for future researches on related factors to be found. Meanwhile the results would help provide insight into design improvements or adaptive management for the alleviation of air pollution.

The B-type cyclin gene, CycB2, serves as a negative regulator of glandular trichome initiation. Through targeted knockout of NtCycB2 in Nicotiana tabacum cv. K326 using the CRISPR/Cas9 system, we created a variety, HK326, which exhibits significantly increased density and larger glandular heads of long glandular trichomes. Under Cd-stress, HK326 exhibited enhanced Cd tolerance, as demonstrated by a robust root system, strengthened cell membrane stability, and higher photosynthetic parameters. HK326 exhibited enhanced Cd-stress tolerance due to a strong excretion capacity of long glandular trichomes by forming calcium oxalate crystals. Cd mainly accumulated in tobacco shoots rather than remained in roots. Specifically, Cd levels of the HK326 shoot surface were nearly two-fold of those of K326, resulting in less Cd internally in the roots and shoots. Gene expression patterns revealed 11 Cd transporter genes that were upregulated after Cd-stress in shoots, roots, and trichomes. Among them, the NtHMA2 gene encoding heavy metal ATPases and involved in the transport of divalent heavy metal cations was expressed consistently and significantly higher in HK326 than K326, both before and after Cd-stress. NtHMA2 expression was strong in trichomes, moderate in shoots, while weak in roots. The results indicate that NtHMA2 may be involved in Cd excretion from glandular trichomes. Our findings suggest HK326 may be an appropriate candidate plant for Cd-stress tolerance.

Traffic-related air pollutants are major contributors to deteriorating urban air quality and pose a serious threat to pedestrians. From both a scientific and a regulatory standpoint, it is important and challenging to understand the contributions of local and non-local sources to accurately apportion specific sources such as traffic emissions contribution to on-road and near-road microenvironment air quality. In this study, we deployed mobile sensors on-board buses to monitor NO, NO₂, CO and PM₂.₅ along ten important routes in Hong Kong. The measurements include two seasons: April 2017 and July 2017. Two types of baseline extraction methods were evaluated and applied to separate local and background concentrations. The results show NO and NO₂ are locally dominated air pollutants in spring, constituting 72%–84% and 58%–71%, respectively, with large inter-road variation. PM₂.₅ and CO largely arise from background sources, which contribute 55%–65% and 73%–79% respectively. PM₂.₅ displays a homogeneous spatial pattern, and the contributions show seasonal change, decreasing during summer. Regional transport pollution is the primary contributor during high pollution episodes. Isolated vehicle plumes show highly skewed concentration distributions. There are characteristic polluted segments on routes and they are most evident at rush hours. The most polluted road segments (top 10%) cluster at tunnel entrances and congested points. Some of these polluted locations were observed in Hong Kong's Low Emission Zones and suggest limitations to the existing control strategies, which only address larger buses. Our work gives new insights in the importance of regional cooperation to improve background air pollution combined with local control strategies to improve roadside air quality in Hong Kong.