Studies have been conducted to find out the effect of the feeding of calves (Bos Taurus) with Jerusalem artichoke (Helianthus tuberosus) concentrate produced in Latvia containing the prebiotic – inulin. The study was performed on two groups of animals - a control group of 8 animals and test (prebiotic) group of 8 animals in summer (from June to August, 2013), and winter (from December, 2013 to February, 2014) in one of cow farm of Latvia, in the municipality of Bauska. Both groups were fed the whole milk, but the test group received additionally 12 g of Jerusalem artichoke powder (an average of 500 g kgE-1 inulin) per day. The overall health status and physiological parameters (temperature, heartbeat and breathing frequency) of both animal groups before the study were of the normal range. After the experiment, we found out that the calves of the test group during both winter (one case) and summer seasons (seven cases), had fewer cases of diarrhea than the control (winter months four cases and summer months nine cases) group animals, the average daily weight gain (control group 0.53 g, prebiotic group 0.75 g) and the total weight gain (control group 29.42 kg, prebiotic group 42.13 kg) during 56 test days was significantly higher ( p is less than 0.05) than that for the control animals. We concluded that the use of Jerusalem artichoke flour concentrate when fed to the calves generally gives positive impact on the development and growth of the calves, improves the status of the gastrointestinal tract and the morphometric indicators.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.

The study was designed to evaluate the immune response of chickens among different poultry management following challenge to live La Sota vaccine and further more investigated the lower antibody response detected in traditionally managed chicken, by testing the hypothesis that veterinary intervention (treatment with anti-helminthic, antibiotics and vitamin supplements), prior to vaccination, would improve the antibody response to live NDV vaccination. Even if, a protective level of antibody (> 4 Log 2HAI) was detected in all chickens following vaccination, antibody titres were significantly higher (p < 0.05) in the intensively managed chickens Geometric Mean Titre (GMT) 85.4 when compared with the traditionally managed chickens GMT 59.3. From multivariable analysis; following a single live ND virus vaccine at the age of 21 day, chickens rearing in intensive production system were 8.6 times more likely to have high titre (128 vs 32) compared to those rearing in backyard free ranging system. The lower ND-HI titre in extensively managed chickens recorded in our study had be explained by impaired immune-competence due to immune-suppressive of concurrent infection. The study investigated the effect of concurrent parasitic and viral diseases on the immune response; as well as differences in protection among indigenous ecotype and exotic breed of chicken. The hypothesis that parasitism contributes to a poorer response to vaccination was proven (p < 0.05). Chickens that received veterinary treatment (de-wormed chickens) prior to vaccination were approximately 14 (i.e. 14.3) times more likely to have a titre of 128 when compared to titre 32 showing evidence for veterinary intervention had detectable effect on humoral response of chicken to vaccination (p< 0.05). Moreover; it was evident that HI titer differed between individual chickens and was influenced by breed types. Multivariate analysis showed that indigenous chickens were about 7 times more likely to have high HI titre (128 vs 32) compared to exotic breeds (p< 0.05). Though breeds differ genetically in their immune responsiveness to the antigen, and being protected following challenge; difference in their susceptibility to the virulent challenge virus needs to be ruled out in order to facilitate the improvement of disease-resistant traits.

The preservation of local animal breeds is a topical theme in recent years. Latvian Brown (LB) and Latvian Blue (LZ) cow breeds are exhausting and must be preserved. It can be performed by the use of multiple ovulation and embryo transfer (MOET). The difficulties exist in choosing the gene-fond (GF) cows as donors caused by the small number of animals. Twenty-three cows were intended for donor cow’s role from different herds until September 2018, and twenty of them were accepted. Anamnesis, clinical examination, blood morphology (13 parameters) and biochemical indices (19 parameters) were analysed before MO induction. Three cows were rejected because of ovarian cysts or pyometra, negative energy balance (NEB) (glucose is less than 2.3 mmol LE−1 simultaneously with elevated ß-hydroxybutyric acid is greater than 1.4 mmol LE−1) and elevated (p is less than 0.05) number of leukocytes (28.20 x109 LE−1). More than 52.6% of cows had a repeated artificial insemination before the last parturition, and 5.3% of cows had lifeless offspring in the last parturition. The 1st and 2nd lactation cows were healthier than older cows (p is less than 0.05). The amount of albumins, cholesterol, triglycerides and Na, K, P, Cl, Mg was significantly different in donors with and without successfully obtained embryos (p is less than 0.05). In conclusion, not only acceptable clinical health but also the cow metabolic status is a decisive factor for success of MOET.

The mainstay of economic activity in Cat Que Commune, Hoai Duc District of Ha Tay Province in Vietnam is got raising, which is the production of piglets between 8 to 30 kg, filling a gap between the sow/piglet and meat pig productions. A cluster of associated enterprises have developed around this got-based enterprise which include the got raisers, piglet suppliers, feed and medicine suppliers, veterinarian services, got collectors, and manure collectors. The relationships with these associated enterprises form the basis of the complex enterprise strategies of the got raisers, which strive to balance a complex set of issues of feed, growth, season, disease control, labour, credit, and marketing in order to ensure the profitability and sustainability of the enterprise. The got are fed a rice-based diet, with occasional protein concentrate to speed up growth for seasons when got prices are high; while inferior feed is given when there is a glut in the market. Disease control is as much related to management as it is to marketing strategy and labour requirements. To sell the whole lot of got yields lower profit and leads to uneven labour requirements, but it provides the opportunity for thorough cleaning of the pig pens and a break in disease; both contribute to better health condition. Selling got on credit fosters relationships with the collectors, but also creates a cash flow problem which in turn is passed on to the feed suppliers who then must sell feed to got raisers on credit. The in-depth analysis of this complex set of interrelated issues provides the basis for elaborating an enterprise plan to improve the system. One research activity that has been completed is a trial on maize substitution for rice and the results showed significant improvement in growth rate (372 vs. 332 g/day of daily weight gain), reduced feed cost (6,013 vs. 7,654 vnd/kg weight gain - USD 1 = 15,400 vnd -), and higher profit (78,611 vs. 51,032 vnd per got). The disease survey also identified the management areas that affect the frequencies of illnesses, including pig pen design and flooring, living space, cleanliness (e.g., bathing frequency and thorough disinfection of pig pens), and drinking water source.

The aim of this study was to determine the influence of Jerusalem artichoke flour feeding on the general health status of calves (Bos Taurus), the animal live weight gain and digestive canal morphological development in the first four months of life, as well as to find out the optimal of three inulin doses. Jerusalem artichoke (Helianthus tuberosus) concentrate produced in Latvia contains the prebiotic – inulin. The study was performed on four groups of animals - a control group of 10 animals and three prebiotic groups, 10 animals in each group. All groups were fed the whole milk, but the PreG6 group animals were fed daily with 6 grams of prebiotic, calves PreG12 group were fed with 12g of prebiotic, but each calf in PreG24 group – 24g of prebiotic per day. CoG control animals did not receive feed supplement. Faecal mass consistence of PreG6, PreG12 groups of animals was more stable within the whole research period than CoG. We found that the control group animal weight gain on the 56th research day is statistically significantly (p is less than 0.05) lower than of those calves that received inulin as a food supplement. The highest growth rates have shown PreG12 and PreG24 group of animals, significantly (p is less than 0.05) being ahead of PreG6 group of calves, the highest average daily live weight gain was observed in PreG24 group (0.95 ± 0.093 kg).

The objective of the study was to investigate the correlation between the milk urea (MU), milk yield and reproductive performance using milk recording tests and the health check results. Weak to average negative correlation was found between MU and the milk yield (r = -0.44 and r = -0.34; p is greater than 0.05), milk protein (r = -0.22; p is less than 0.05) and milk freezing point (r = -0.33; p is less than 0.01). Higher milk fat was found in cow milk with MU below 30 mg dLE-1 in comparison with cow milk which had MU above 30 mg dLE-1, 4.0 ± 0.77% and 3.9 ± 0.75%, respectively. High MU fluctuations within a cow group can be observed if cows of different productivity levels are grouped together or if there are too many animals in one group. Correlations between MU and times of artificial insemination or the service period were not established, possibly due to successful veterinary measures. In conclusion we emphasize the necessity on large dairy farms to analyse MU concentration in relation to milk protein and fat concentration for individual animals and in each feeding group separately. The study was the part of the State Research Project (AgroBioRes) No. 2014.10-4/VPP-7/5; subproject VP29.

Blood haematology is one of the indicators that shows if a goat (Capra hircus) kid gets a sufficient quantity of liquid, has a good supply of oxygen in the body, as well as an indication of inflammatory processes. Our aim of this research was to find out how feed additives (probiotics (Enterococcus faecium), prebiotics (Jerusalem artichoke (Helianthus tuberosus L.)) and symbiotics (Enterococcus faecium+ Jerusalem artichoke)) influence on haematological parameters of kids. Research was performed in Latvia in 2014. The blood samples were analyzed for leucocytes (WBC), erythrocyte number (RBC), haemoglobin (HGB) concentration, packed cell volume (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentrations (MCHC). Since the blood indicators have not significantly changed in all examination times, we analyzed the results of the age of 4, 8 and 12 weeks. HCT downward trend in blood sample MRG + PRO, MRG + PRE and MRG + SIM group of kids was observed from the fourth week up to the eighth week and then it gradually increased. The results showed that the HCT, HGB, RBC, MCHC and WBC number was comparable (p is greater than 0.05) between groups. Our results prove that the use of Jerusalem artichoke powder as an additive to food not only improves digestion and metabolism of ruminant, but also helps to maintain constant haematological values. In the control group and the kids who received probiotics, prebiotics or symbiotics - significant differences are evident in all haematological parameters (p is less than 0.05) at the age of 12 weeks.

The productivity, health and welfare of cows are considerably influenced by the microclimate of the cowshed in which they live. The present paper deals with the spatial variation in temperature within uninsulated wooden cowshed and cowshed renovated from stanchions into cubicles. The type of the cowshed (uninsulated wooden cubicle cowshed and renovated cowshed made of concrete elements) influences spatial variation of indoor temperature. Greater spatial variation existed in renovated cowsheds, but these remained within the range of cows' temperature comfort zone.