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Microbiological quality of cows’ milk in organic farming (preliminary report)
2012
Gulbe, G., Latvia Univ. of Agriculture, Jelgava (Latvia) | Valdovska, A., Latvia Univ. of Agriculture, Jelgava (Latvia)
The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.
显示更多 [+] 显示较少 [-]Elaboration of solid phase extraction method for analysis of sterigmatocystin
2007
Versilovskis, A., Latvia Univ. of Agriculture, Jelgava (Latvia) | Mikelsone, V., Latvia Univ. of Agriculture, Jelgava (Latvia)
Sterigmatocystin is a mycotoxin produced by fungi of many Aspergillus species and it is a biogenic precursor of aflatoxin B1. For analysis of various mycotoxins to clean up sample extracts, mainly solid phase extraction (SPE) is used. An elution of sterigmatocystin from Strata X and Strata C18 SPE columns by different acetonitrile-water and methanole-water solutions were checked in this paper. Acquired results showed a possible suitability of both columns for the analysis of sterigmatocystin.
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