Maize is increasingly used for energy production in agricultural biogas plants. The first biogas production project in agriculture is realized in the Research and Study Farm (RSF) Vecauce of the Latvia University of Agriculture in 2008 and animal manure together with plant biomass are planned to be used as substrate. The paper aimed to evaluate ten maize hybrids possibly suitable for biomass production at three different harvesting times. Field trial was carried out in Research and Study farm Vecauce of the Latvia University of Agriculture in 2008. Ten maize hybrids with different maturity rating according FAO numbers (FAO 180 - 270) were harvested at three different times beginning on 5 September at fourteen days intervals. Results showed that harvest time effect on maize yield was substantial (p is less than 0.05), but hybrid influence on the average yield per all harvest dates was not substantial (p is greater than 0.05). The highest organic dry matter yield was obtained harvesting maize on October 3. The Total N, crude ash, protein, fibre, cellulose, lignin, neutral detergent fibre (NDF) and acid detergent fibre (ADF) concentration decreased, but crude fat concentration increased during plant development. Relevance was not noticed between harvest dates and total carbon and hemi - cellulose content. The C : N ration rose from 34.76 at first, early harvest on 5 September to 37.97 at the last harvest on 3 October.

The utilization of traditional fossil fuels (oil, gas) as primary energy resources causes a destabilization of the eco-environmental situation in the world. Latvia has to significantly decrease energy imports from its neighbouring countries. This can be achieved by using high-quality local primary renewable energy sources. One of the solutions is to utilize anaerobic fermentation for biogas production. This process can be ensured by utilizing manure, food waste as well as energy biomass - wood, grass and maize. Biogas is utilized as a primary energy source in a cogeneration plant which is a combined cycle plant for electricity and heat power production. Microcogeneration plant for farming household needs would ensure an independent power supply, in case the overall electrical network is in a state of emergency. In order to ensure optimal biogas yield, which, in turn, would ensure a stable operation of the microcogeneration plant, it is important to know the parameters and quality of the biomass that have been filled in bioreactor. This research deals with the influence of the linkage biomass type on the qualitative and quantitative indicators of biogas. As a result, it has been found that biomass type affected the methane percentage in biogas greatly. The methane content of biogas independent with biomass type was diminished from 65% (galega) to 44.5% (fresh sawdust), but biogas yield decreased from 0.627 m**3 kgVSd E-1 (galega) to 0.185 m**3 kgVSd E-1 (fresh sawdust).

Prolonged summer drought periods are forecasted for the Baltic Sea region during the 21st century, thus increasing the risk of drought stress of saplings used in forest regeneration. Nevertheless, the vitality of young stands might be increased by the selection of suitable planting material. The aim of this study was to estimate the effect of changes in distribution of summer precipitation on height increment, biomass distribution and root morphology of Scots pine and silver birch planting material commonly used in the forest regeneration in Latvia. Containerized pine and bare rooted birch saplings, planted in three different soil types, were subjected to altered distribution of summer precipitation, provided by the use of automated shelter. Sheltered saplings were weekly irrigated with the sum of precipitation of a corresponding period, while afield planted saplings had an unchanged precipitation regime and served as control. Height increment was measured once per week and estimation of morphology of saplings was done after the end of every vegetation season. Significant (p is less than 0.05) differences in height increment, and shoot and root biomass were observed among the same planting material in different irrigation regimes and soil types. In the control plots of peat soil, pine had a significantly (p is less than 0.05) larger height increment while birch-significantly (p is less than 0.05) smaller compared to experiment. Forecasted longer drought periods might reduce the growth of Scots pine in fertile forest types but silver birch growth might be affected in fertile mineral soils in future.

The stump lifting trials were implemented in 5 forest compartments of the JSC ‘’Latvia state forest’’ Vidusdaugava, Rietumvidzeme, Zemgale and Ziemeļkurzeme forestries in autumn, 2012. Total extracted area was 3.5 ha, excluding control. Two stump extraction buckets were compared in these trials – CBI (made in Canada) and MCR-500 (made in Latvia). The scope of the study was to estimate if the prototype of the MCR-500 can compete with stump lifting buckets having positive feedback from industry. Considering that the CBI head cannot prepare soil, this operation was not done by the MCR-500 either. In total 1796 stumps were marked and their main parameters were taken in all trial areas. Extracted biomass was estimated theoretically using biomass expansion factors elaborated in Nordic countries. Allegro CX field computers with SDI software were used in time studies to obtain information about productivity and distribution of productive time in a work cycle. The study demonstrated that productivity of stump extraction with both stump lifting buckets did not differ significantly in 6 cases out of total 10 comparisons.

Tree roots take up a high proportion of forest biomass, and nowadays we use different methods to estimate the root biomass. Methods vary greatly due to the different studies and different excavation methods used. In the summer 2010, a study of the below-ground biomass of young Scots pine Pinus sylvestris L. stands was carried out in central Latvia. In this paper, different below-ground biomass fractions data of 10 sample trees from the abandoned agricultural land plantations were approximated by allometric functions depending on tree stem diameter at breast height 1.3 m (DBH). The main objective was to determine the average below-ground biomass and approximated below-ground biomass fractions by allometric functions depending on tree DBH of young stands of Scots pine on abandoned agricultural land. Our regressions offer good overall approximations of the data. DBH – stump, coarse root and small root and total biomass regressions were highly significant (p is less than 0.001). The most substantial part of total below-ground biomass was from stumps (45%). The smallest shares of the biomass were coarse roots 38% and small roots 16%. The largest portion (52%) of the total fine-root biomass of 12- and 14-year-old Scots pine stands was located at a depth of 0–10 cm, decreasing in deeper mineral soil layers. Total dry (DM) below-ground biomass including fine-root biomass was 19.0 ±5.2 t DM ha-E1.

In the summer 2010, a study of the below-ground biomass of young (12 - and 14-year-old) Scots pine Pinus sylvestis L. stands was carried out in central Latvia. The fine-root (diameter is less than 2 mm) biomass, tips, length and volume were determined for the mineral soil layer (0 – 60 cm) by core sampling. The main objective was to determine the average fine-root biomass and morphological characteristics in different classes of young stands of Scots pines. The mean fine-root biomass was calculated for the whole stand by using below-ground biomass measurements of different components of sample trees and measurement of the tree stand. The amount of dry fine-root biomass in the soil layer was 5.3 ± 1.6 t haE-1 in the 14-year-old stand and 3.6 ± 1.5 t haE-1 in the 12-year-old stand. The study showed that the largest portion (52%) of the total fine-root biomass of 12 - and 14-year-old Scots pine stands was located at a depth of 0–10 cm, decreasing in deeper mineral soil layers. The average morphological characteristics of fine roots were higher in the older stand. Analysis of the morphology of fine roots showed that at soil depth of 0 – 10 cm the mean root length was 233 ± 44 cm, volume – 1.3 ± 0.6 cm3, value of tips – 537 ± 104 and diameter – 0.7 ± 0.1 mm per 100 cmE-3.

Considering specific role of forest in carbon cycling, the scope of the study is evaluation of assimilation of carbon dioxide in a single grey alder stand. The National statistical forest inventory demonstrates that total area of afforested farmlands is 314 thousands of ha, including 212 thousands of ha are grey alder stands. Empiric data are collected in 2011 in 15 years old grey alder stand representing Aegopodiosa site type, site index II. Dendrometric characteristic of the stand are estimated using a method of 6 sample tree plots. Average height of dominant trees is 9.6 ± 0.14 m, diameter at breast height - 6.7 ± 0.18 cm, volume of stem - 0.02002 ± 0.00673 m3, number of trees per ha – 5806 ± 560, growing stock - 116.2 ± 20.0 m3 haE-1. Density of the grey alder stem wood is 411.0 ± 2.2 kg mE-3, average relative moisture - 51.6 ± 0.13%. Dry biomass of grey alder in the evaluated stand is 73.4 tons haE-1, including stem biomass - 65.3%, branches - 11.1%, leaves - 2.3%, stump - 6.8% and roots - 14.6%. In average evaluated stands accumulated 36.9 tons haE-1 of carbon removing from atmosphere 135.5 t ha-1 of CO2. Wood density is estimated according to ISO 3131:1975 standard, moisture content – according to EN13183-1:2002 standard.

Computer models of bioprocesses have become an essential tool in biotechnology. This paper describes various bioprocess scaleup releated problems. The problems at different scales are explained. In this paper the use of computer model in E.coli fermentation scale-up from shake flask to laboratory scale bioreactor is discussed. A black box modelling approach was used. Fermentation results have been visualized and discussed. The computer model created in Matlab environment was used for bioprocess behaviour prediction. Possible bioprocess scale-up software improvements and bioprocess optimization are discussed.

Bioenergy production growth rates in the recent years are leading to waste – digestate and wood ash amount increases, which are essential to be managed in the most efficient and safe way. In the formation of plant nutrient recycling these waste products are useful to be included in the energy crop fertilisation plan. In order to study the waste products application options for energy crops – reed canary grass (Phalaris arundinacea L.) and festulolium (×Festulolium pabulare) fertilisation trials were arranged in 2012 at the Skriveri Latvian University of Agriculture (LLU) Research Institute of Agriculture. In all fertiliser treatments: wood ash; digestate one time per season; digestate two times per season and mineral fertilisers the same doses of the main plant nutrients (N, P, K) were provided, the missing quantities of elements compensating with mineral fertilisers. To obtain the grass biomass, two cutting regimes were used – two-cut and one-cut harvest system. This article summarizes the findings on the productivity of the 1st year of use grassland swards and sward structure data. The productivity of perennial grass biomass was dependent on the type of applied fertilisers, grass species and cutting regime. In the first production year the highest average dry matter yield (7.30 t haE-1) was produced by reed canary grass. The highest DM yields in average for both grass species were obtained by mowing once per season – in autumn at crop senescence (7.01 t haE-1) and in fertilisation treatments of wood ash (WA) – 8.06 t haE-1 and mineral fertilisers (MF) – 7.24 t haE-1.

Europe’s future wood demand for energy is expected to increase by 10 million to 200 million m3 in the period 2010- 2030. This will be supplied by both domestic sources (forests, industrial residues post-consumer wood waste), but also from sources outside Europe. The EU-28 predicts a near future (2020) gap between solid biomass supply and demand for renewable energy: 21.4 million tonnes of oil equivalents (MTOE). This is estimated via preliminary renewable energy action plans (NREAP’s) per country. The EU-28 expects wood pellet import will merely complete this gap of 21.4 MTOE, with more than 50 million tonnes of pellets. This implies a feedstock need of 125 million m3 of wood from forests and other sources outside the EU-28. A practical approach to include bioenergy in wood sector models should start with the input of wood pellets. Ideally, three types of bioenergy markets should be considered, in which pellets and the other major woody feedstock are included: 1. Large scale power production (the UK, Belgium, the Netherlands, all importing pellets from outside the EU-28); 2. Medium scale combined heat and power (CHP’s) including those in the forest sector (Nordic countries use pellets and chips for energy, merely imported from the EU-28); 3. Small scale residential heating (Germany, Austria and Italy, using wood pellets and logs from regional sources). We suggest starting with inclusion of medium scale CHP’s, followed by large scale power production. Small scale heating is relatively stable and should not have large impacts on future markets.