The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The main purpose of the present experiments was to investigate the effect of various hydrogen peroxide (H2O2) concentrations and for various lengths of treatment on the total carotenoid, β–carotene content, colour intensity and microbiological safety on the fresh shredded carrots. Shredded carrots were dipped in 0.5, 1.0 and 1.5% H2 O2 water solution for 30 ± 1s, 60 ± 1s and 90 ± 1s. Negative effect of H2 O2 on β–carotene content and colour parameters of analyzed shredded carrots samples was not detected. In carrots treated with H2 O2 (p = 0.008) for 60 – 90s the total content of carotenoids significantly decreased during treatment compared to untreated carrot samples. There was significant difference (p is less than 0.05) observed between treated and non–treated shredded carrot samples on the total bacteria count. It was possible to reduce significantly (p is less than 0.05) the content of yeasts and mould up to 99.99% by shredded carrots treatment with 1.5% hydrogen peroxide water solution for 30 ± 1s. In the non–processed carrots E.coli was detected; however, it was possible to destroy E.coli by treating carrots with 0.5% H2 O2 water solution for 30 ± 1s. Considering all experimentally obtained results, we have concluded that fresh shredded carrots could be treated in water with the addition of hydrogen peroxide 1.5% for 30 ± 1s to maintain quality.

Computer models of bioprocesses have become an essential tool in biotechnology. This paper describes various bioprocess scaleup releated problems. The problems at different scales are explained. In this paper the use of computer model in E.coli fermentation scale-up from shake flask to laboratory scale bioreactor is discussed. A black box modelling approach was used. Fermentation results have been visualized and discussed. The computer model created in Matlab environment was used for bioprocess behaviour prediction. Possible bioprocess scale-up software improvements and bioprocess optimization are discussed.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.

The feature of the microorganisms of rumen microbiocenosis is the ability to form a number of digestive enzymes, including cellulases, which allows ruminants use the energy of feeds rich in fiber. Microorganisms that are promising as a source of cellulases, biodestructors of toxins having antagonistic properties against pathogens for creating probiotic feed additives, both for reindeer and for other livestock, were isolated from the reindeer rumen. The aim of the study was to create a collection of microorganisms that are promising for creating a biopreparation. As a result, collections of 63 associations of microorganisms decomposing cellulose and carboxymethylcellulose were created; their morphology and cultural properties were described. It was found that the amount of cellulose degradation was 44 – 62% in the 4 most active isolates (No 14, 15, 21 and 26). The obtained data allowed to make a conclusion about the ability of bacterial strains isolated from the rumen to synthesize cellulolytic enzymes carrying out cellulose biodegradation, which allows them to gain a competitive advantage in the rumen of the reindeer diets with an abundance of cellulose. Isolates No 14 and 15 showed high antagonistic activity to Fusarium sporotrichioides, and isolate No 15 and 16 showed high antagonistic activity to Fusarium oxysporum. Isolate No 15 showed a wider antifungal activity compared to other isolates. Among all isolated strains, this bacterial isolate is represented as having a large spectrum of activities, including both high cellulolytic and high antifingal activity.

The aim of the study was to assess the ability of pathogens metabolic repair from injury within 10 days of refrigerated storage of milk after high pressure treatment. Two pathogenic strains – Listeria monocytogenes ATCC 7644 (LM) and Escherichia coli ATCC 25922 (EC) were inoculated in ultrahigh-temperature treated (UHT) milk at concentration of about 107 CFU mLE-1 and treated at 400, 500, 550, and 600 MPa for 15 min with inlet temperatures 20 °C, and then stored at 4 ± 2 °C to evaluate survival and growth of pathogens. By increasing the applied pressure, an increased rate of the pathogens’ inactivation was achieved. After 10 days of storage, milk treated at 400 MPa showed growth over 3.5 log CFU mLE-1 of L. monocytogenes and 1.7 log CFU mLE-1 of E. coli. In 550 MPa and 600 MPa treated milk samples after 8 and 10 days of storage colony formation occurred (3 CFU mLE-1 (550 LM) and 2 CFU mLE-1 (550 EC, 600 LM and 600 EC)). Although high pressure treatment is effective method for reducing of pathogenic bacteria, the metabolic repair from injury of bacterial cells in milk during storage should be considered.

A study was conducted to determine efficiency of a phytoadditive on pig growth processes and digestive tract microflora. The pigs of control group were fed without the phytoadditive. The feed of the trial group piglets contained 0.5% of the phytoadditive per tonne feed, for starter pigs and finished pigs - 0.2% per tonne feed. The study indicated that at the age of 170 days, pig mass in the trial group was 111.67+-1.22 kg on average, but in the control group - 101.79+-0.81 kg, which showed that pigs from the trial group had by 9.7% higher average mass than in the control group (p is less than 0.05). Average daily gain for the trial group was 0.777+-0.009 kg, which was by 12 % more than for the control group pigs (p is less than 0.05). Feed conversion in the trial group was 2.928 kg, but in the control group - 3.129 kg, which was by 6.4% higher than in the trial group. Gastric microflora analyses showed that use of phytoadditive reduced mould colony forming units (CFU) amount in the trial group decreased by 24 times. Duodenum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 25%, yeast CFU amount by 34%, Escherichia coli mesophilic and termophilic forms CFU by 16.3% compared to the control. A lactic acid bacterium CFU in the trial group was 2.5 times higher compared to the control. Rectum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 31.6%, yeast CFU amount - by 62%, Escherichia coli mesophilic and termophilic forms by 57 % and 15.6 % respectively. Lactic acid bacteria CFU amount in 1 g of sample in the trial group increased by 5.1 times.

This article presents the results of a comparative evaluation of preventive treatment of udder with probiotic agents: ‘Dipal’ (manufacturer DeLaval - Sweden), ‘Zorka’ (manufacturer «NPP Farmaks» - Russia). We have received data on the effects of probiotic preparation on quality of milk and number of somatic cells in milk. Research has been conducted in two dairy farms of Almaty region of the Republic of Kazakhstan. Lactobacillus acidophilus 05ch - isolated from shubat (South Kazakhstan region, Turkestan). Lactococcus lactis 010k - isolated from three-day kumys (Zhambyl region, Merke). These probiotics are used as a means for sanitary treatment of the udder of cows. Thus, the procedure of determining the antagonistic activity revealed their high activity against both gram-negative and gram-positive microorganisms, notably Staphylococcus aureus (10-13 mm), Escherichia coli (12 mm), Proteus vulgaris (10-14 mm), Salmonella abortus ovis (11-13 mm). Significant changes in the milk indices can be found in the experimental group: 9.1% rise of lactose, and 7.2% reduction of protein. The level of protein increased by 4.1% , whereas the amount of lactose, on the contrary, went down 1.6%, which may be indicative of the increase in the content of serum protein when the udder is inflamed. The examination of the milk obtained from the cows of the control and experimental groups showed a nearly double reduction in the number of somatic cells from 488.00 down to 178.000 thousand, which was caused by the daily treatment of cows by probiotic cultures during 2 weeks.