Scots pine (Pinus sylvestris) is Latvia's most economically important tree species. It accounts for 38% of the total forest area of Latvia. One of diseases affecting P. sylvestris is root rot caused by the fungus Heterobasidion annosum, which causes large economic losses. There is some evidence of Scots pine trees with higher levels of resistance but no absolutely resistant tree clones have been described so far. Many genes encoding peptides and proteins with direct or indirect antifungal activity have been described in various plant species, but only few of them have been studied in conifers. In our study we have utilised various approaches to research genetic aspects of Scots pine resistance to H. annosum. Here we present our initial results. Initially, H. annosum infection was determined in 300 trees and in a subset of twenty seven trees representing fifteen families (progeny of one mother tree) infection levels were quantitatively characterised. Candidate-genes were selected based on previously published research. Gene copy number variation (CNV) analyses were performed on selected samples. Copy number variation polymorphism was detected for a gene encoding a thaumatinlike protein analogues of which are described as potent antifungal proteins in other plants. As increased gene copy number can lead to increased gene product amounts in cells it is possible that an increased copy number of thaumatin-like protein is beneficial to the pine tree in respect to resistance against H. annosum and other pathogens. Further experiments need to be performed to investigate this in more detail.

The impact of climatic and genetic factors on needle cast damage, as well as relationship between the degree of damage and survival and growth of trees was evaluated in the study. The analysis was done in two series of experiments, both established in two locations in Latvia (central and eastern part). Needle cast damage (in 5 grade scale) as well as the height and height increment, diameter of root collar and survival of trees were assessed. Results suggest that the degree of needle cast damage was influenced by the genetic, as well as climatic factors, and also their interaction. The average degree of needle cast damage in experiment of open-pollinated families was 4.7 ± 0.01 in central and 4.6 ± 0.03 in eastern Latvia; in the experiment of control-crossed families it was 4.6 ± 0.02 in central and 3.4 ± 0.04 in eastern Latvia. The impact of genetics on the needle cast damage degree was statistically significant (p is less than 0.05), and this effect was observed when any of growth traits (diameter, height or height increment) was used as a covariate in analysis. Results suggest the potential to select more resistant families, since the genetic correlation of grade of the damage between locations was significant (r = 0.40 - 0.72) and therefore improve also the growth of trees, since the grade of the damage had a negative correlation with growth traits both at provenance and family mean level (r = -0.69 and r = -0.42; p is less than 0.01).

Puccinia striiformis is a biotrophic pathogen able to cause broad scale epidemics in wheat growing regions. P. striiformis is genetically highly variable pathogen. New, aggressive genetic lineages, adapted to warm temperatures have been observed in the last decades worldwide. The study aimed to ascertain the structure of genetic lineages of P. striiformis in Latvia. Forty one wheat leaf samples with yellow rust symptoms were collected in 2017–2019. Fenotyping and genotyping methods were used for identification of genetic lineages in Global Rust Reference Center, Denmark. Assessments of leaf diseases on winter wheat differentials – ‘Ambition’, ‘Mariboss’, ‘Moro’, ‘Compair’, ‘Rendezvous’, ‘Spalding Prolific’ and local variety ’Fredis’ were made during the research. Five genetic lineages of P. striiformis – PstS4, PstS7, PstS10, PstS13 and PstS14 were found. 56% from the samples belonged to PstS14, 17.1% PstS10, 12.2% PstS4 and PstS7, 2.4% PstS13. Genetic lineages identified from Latvian wheat samples are found in the biggest cereal growing regions in Europe and are able to cause epidemics on wheat. Genetic lineages of P. striiformis from Latvian samples have not been identified before. All differential varieties were infected with P. striiformis in 2017, ‘Ambition’ and ‘Moro’ in 2018, no infection was observed on differentials in 2019 despite the presence of P. striiformis on winter wheat variety ‘Fredis’. The identification of genetic lineages of P. striiformis on wheat in Latvia is necessary to continue.

Grain samples of 51 spring barley (Hordeum vulgare L.) genotypes, including two-row, six rows, covered and hull-less were used for investigation of endosperm beta-glucan content. A significant difference (p-value is less than 0.001) between genotypes in beta-glucan content was observed. On average, the six-row barleys had slightly higher beta-glucan content. Far six-row genotypes it ranged from 35.9 to 47.1 mg kgE-1 with the mean value of 41.4 mg kgE-1, but for tw0-row barleys - 38.0 mg kgE-1 within the range of 33.2-44.9 g kgE-1. Six-row genotypes had a higher coefficient of variation in comparison with two-row genotypes. Hull-less genotypes had significantly higher beta-glucan content than the covered ones (49.3 mg kgE-1 and 38.9 mg kgE-1 respectively). The standard deviation of the mean values of beta-glucan content for covered genotypes was lower than for the hull-less ones. The mean values in 2005 were well plotted against data from the previous season for genotypes included in the two years trial. There were two distinct clusters of covered and hull-less genotypes. Beta-glucan content for varieties bred in Latvia ranged from 33.2 mg kgE-1 for two-row variety 'Malva' to 46.0 mg kgE-1 for six-row variety 'Druvis'.

Modern varieties developed under conventional plant breeding programs do not always perform well under organic and low-input growing conditions. Therefore, organic farmers need varieties adapted to variable environmental conditions. This means emphasis on traits such as nutrient uptake and use efficiency, good weed suppression ability and disease resistance and can be achieved via extended genetic diversity within the varieties: variety mixtures and populations of self-pollinating crops. The aim of the present study was to review published scientific literature about importance and research results on this topic. Scientific articles about the necessity of such studies and results of investigations performed in different countries have been studied. The main results show that variety mixtures have advantages in terms of disease control and yield stabilization and performance of mixtures may be affected by various factors – choice of components and its number, interactions between plants of components, manner of the mixing and growing technology. Depending on parental material, populations contain greater genetic diversity than variety mixtures. There are trends observed in the studies that yield of populations increased over generations and they are more stable than modern varieties, but there is no evidence that they would be significantly more productive. This research direction has recently become topical and is not sufficiently widely covered in the world agricultural science. The obtained results are inconsistent.