The changes in the serum immunoglobulins concentration were investigated in newborn heifers from birth to 7 days of age. The mothers of calves were determined serum and colostrum immunoglobulins (Ig) concentration. The research was carried out at the Latvia University of Agriculture (LLU), in dairy farm ''Ligotnes'' of the Research and Study Farm ''Vecauce'', in a loose housing system with 530 cows, of which 430 were milking cows. The cows during the dry period were kept tethered in the farm. Eighteen heifers and their dams (Latvian Brown and Holstein breeds) which calved from 30 November 2010 to 23 January 2011 were used in this study. The calves' serum total immunoglobulin concentration at birth was 9.0 ±1.31 mg dLE-1 (or 0.09 mg mLE-1), and after 24 hours (h) it increased significantly to 4.3 mg dLE-1 (or 0.043 mg mLE-1), p is less than 0.05. The immunoglobulin G (IgG) concentration in calf serum after birth was 6.8 ±1.50 mg dLE-1 (or 0.068 mg mLE-1), and after 1 day and after 7 days it did not change significantly. The average Ig serum concentration in cows was 12.6 ± 1.36 mg dLE-1(or 0.126 mg mLE-1), and Ig concentration in colostrum was 18.5 ± 1.68 mg mLE-1. On average, close correlation (r=0.56) was found between cow blood serum IgM and colostrum IgM. Correlation between cow and calf IgG serum concentration proved to be weak (r=0.49).

Bovine colostrum is a mammary gland secret which, due to its high immunoglobulin concentration, is necessary for the transfer of passive immunity to the calf, preventing diseases caused by microbial infections in the newborn ruminants. Colostrum, however, may contain pathogens and can be an infection transmitter, affecting morbidity and mortality rates of calves in the farms. Total plate count and immunoglobulin concentration are two main factors affecting colostrum quality, therefore the aim of the study was to analyse Latvian dairy herd colostrum quality. Colostrum was collected from Holstein Black cows within the first six hours after calving, lactation period of animals ranged from 1st to 4th lactation. Colostrum samples (n=51, 50 mL) were collected from December 2018 to February 2019. Immunoglobulin concentration (n=51) was defined by colostrometer (COLOSTROMETERtm Biogenics, USA), total solids content by optical refractometer (Model BX, UK). Staphylococcus spp. colony–forming unit (CFU) (LVS EN ISO 6888-1+A1:2007), the presence of Listeria spp. (LVS EN ISO 11290-1+A1:2007) and Salmonella spp. (LVS EN ISO 6579-1:2017) were examined in the colostrum samples (n=20). Despite the high immunoglobulin concentration in the analysed samples, our research findings demonstrate suboptimal colostrum quality received by calves. That indicates the necessity for regular colostrum quality control and better management practise providing on the farm.

Natural defence mechanisms of the mammary gland tissues play a vital role in protecting the gland from infections. The progress of mammary infection depends on the ability of bacterial pathogens to adapt to milk and udder tissues, and on the various virulence factors they activate, as well as on the cow's response. The levels of immunoglobulins (lg) and lactoferrin (Lf) concentration in the milk from dairy cows with and without subclinical mastitis were determined. In this investigation it was stated that 82.5% of samples were negative, but 17.5% of samples were positive for pathogens. Cows had subclinical mastitis caused by Coagulase negative staphylococci observed in 48.60%, Streptococcus uberis - 32.10%, Staphylococcus aureus - 18.90% of cases in milk samples. There were no significant differences between all classes of immunoglobulins concentration in the milk without pathogens and with pathogens. The lactoferrin concentration was significantly increased for 40.33% in the milk with pathogens.