Raw milk cheeses are known to have more intense and strong flavour and different texture due to natural microbiota and enzymes. Nevertheless, there are concerns about safety of these products. For microbial inactivation heat treatment of milk is used, but it can adversely affect the flavour, taste and texture of the product. Therefore, applying non-thermal technology such as high pressure processing is attracting alternative. The aim of this study was to examine the effects of high pressure treatment of cow`s milk at a wide range of pressures (400–600 MPa) on milk rennet coagulation time, curd firmness and curd yield. Processed milk samples were subjected to enzymatic coagulation using commercial rennet to determine rennet coagulation time, yield of coagulum and curd firmness. High pressure processing insignificantly influences coagulation properties of whole milk. However, the magnitude of changes depended on applied pressure. Rennet coagulation time and curd yield were significantly different (p is less than 0.05) among the pressure treated milk samples. The higher firmness of the curd form pressurized milk than that of raw or pasteurized milk, evaluated positively. The main effects of high pressure treatment in milk appeared to involve dissociation of casein micelles from the colloidal to the soluble phase. This study suggests that high pressure treatments of milk at 500 MPa or 550 MPa for 15 min may be beneficial for improving the coagulation properties of milk. These positive effects indicated that high pressure processing may have potential for new cheese varieties development.

The diversity of non-starter lactic acid bacteria in raw, pasteurised milk and maturated Swiss type cheese was tested. The aim of the present study was to analyse the concentrations and representatives of non-starter lactic acid bacteria in raw milk and to evaluate the changes of their concentrations and representatives during pasteurisation of cheese milk and Swiss-type cheese production. The analysis carried out in the study showed a variation in the microbial composition and quantity of raw milk. The most frequently isolated lactobacilli were found at low level in raw milk (mean 27.5×104 CFU mLE-1) and the most frequently identified species were Lactobacillus brevis and Lactobacillus paracasei. The microflora of raw and pasteurised milk is similar analysing lactic acid bacteria representatives in the samples. Lactobacillus brevis and Lactobacillus paracasei were detected in the same samples in raw milk, pasteurised milk and maturated cheese. Our study showed that lactic acid bacteria concentration was quite low in pasteurised milk (1-12 CFU mLE-1), but they grow rapidly in cheese during ripening reaching 1.1-1.8*106 CFU gE-1. The present study has shown that NSLAB in Swiss-type cheese mainly derive from raw milk, and only a few strains survive the processing conditions and grow during ripening.

In this study we described the diversity of lactic acid bacteria and their representatives in raw and thermally treated milk, focusing on their potential in cheese production influencing cheese quality. The aim of the present study was to analyse the concentrations and representatives of lactic acid bacteria in raw milk and to detect the changes of lactic acid bacteria microflora during thermal treatment of cheese milk at a dairy processing plant. The analysis carried out in the study showed a seasonal variation in the microbial composition and quantity of raw milk. The most frequently isolated lactic acid bacteria: lactococci, lactobacilli, leuconostoc were found at low level in raw milk (mean 9.27×103 CFU mLE-1) and the most frequently identified species were Lactococcus lactis, Lactobacillus brevis and Lactobacillus fermentum. The microflora of raw and pasteurised milk is similar to the analysed lactic acid bacteria representatives in the samples. Interestingly, we found the same species in raw milk and pasteurised milk, for example, Lactobacillus brevis and Lactobacillus fermentum were detected in the same samples in raw milk and pasteurised milk. Our study showed that lactic acid bacteria concentration was quite low in pasteurised milk (0-76 CFU mLE-1), but they grow rapidly in cheese during ripening; therefore the definition of limits of the non–starter lactic acid bacteria colony forming units in milk should be reasonable for selection of appropriate raw milk quality for cheesemaking.