Human milk provides infant with the required nutrients for growth and development. The aim of preliminary study was to determine macro- and micronutrients content in mature human milk among lactating women residing in Latvia and dietary habits affecting it. The study was carried out from November 2016 to February 2017. In total, 24 pooled diurnal milk samples were collected from mothers whose infants had reached the age of at least two months. Personal information of each participant was recorded, including mother’s age, weight and height parameters, sex and age of an infant, parity, breastfeeding pattern, milk expression method used for sampling. Eating habits were obtained using Monthly Food Frequency Questionnaire. Analysed elements were determined using inductively coupled plasma mass spectrometry (ICP‑MS Agilent 7700x, Japan). Concentrations ranged from 227.52 to 398.34 mg LE-1 for calcium, 58.56 – 256.38 mg LE-1 for sodium, 445.33 – 736.71 mg LE-1 for potassium, 25.73 – 49.52 mg LE-1 for magnesium, till 3.17 mg LE-1 for iron, 0.28 – 2.77 mg LE-1 for zinc, 0.06 – 0.43 mg LE-1 for copper, 2.00 – 44.00 µg LE-1 for manganese, 1.00 –10.00 µg LE-1 for chrome which is comparable to data obtained from other studies although chrome and manganese concentration was even higher than observed in literature. Cobalt and selenium content was below detection limit. Zinc content in human milk negatively correlated with baby’s age (p is less than 0.05). The content of majority of essential minerals in human milk was affected by mother’s dietary habits; however, more samples need to be analysed for representative conclusions.

Kvass is a non-alcoholic beverage produced by fermenting kvass mash with yeast; alcohol content in kvass must be less than 1.2% by volume. Kvass extracts have longer shelf-life and they are essentially free of ethanol. The aim of this research was to evaluate and compare aroma compounds in naturally fermented kvass and kvass extracts. Experiments were carried out at the Latvia University of Agriculture, Department of Food Technology from November 2014 to February 2015. Three commercially available kvass samples (Bruveris, Bauskas and Liepkalni) were used to produce kvass extracts applying vacuum evaporation. The investigation of volatile compounds in kvass and kvass extracts was performed using solid phase microextraction and gas chromatography mass spectrometry. Dry matter content in kvass extracts was 32.4 ± 0.3% (ISO 2173:2003). In all kvass and extract samples in total 25 volatile compounds were detected. Ten of them were esters, five alcohols, five acids, four aldehydes and three ketones. Such aroma compounds as ethyl acetate (fruity flavour), hexyl acetate (fruit, herb) and ethyl decanoate (grape) were found only in Bruveris kvass, 2,3-butanedione (buttery) and phenethyl butyrate (floral) were found only in Bauskas alus kvass and three volatile compounds were identified only in Liepkalni kvass – acetic acid (sour), furfuryl alcohol (burnt) and carvone (caraway). Less than a half of the main aroma volatiles in kvass were also identified in kvass extracts and total values of peak areas were significantly lower in kvass extracts compared to kvass (p = 0.01).

Rosemary (Salvia rosmarinus) is an aromatic herb with a multitude of uses. It is both a seasoning in various dishes, and a natural remedy for several diseases mainly due to its anti-inflammatory and anti-oxidative effects. Plus, it is also used in cosmetic industry and for improving agriculture practices and helping the environment, since it may be a natural antimicrobial. It is worth noting that this plant is also grown and commercialized in Uzbekistan, thus, if added value is given to rosemary’s products, it has potential for improving living conditions and helping in the economy of local farmers and entrepreneurs. In this work, we discuss a technique for obtaining rosemary essential oil, rich in terpenes, by vacuum assisted hydrodistillation. This is a solventless environmentally friendly and safe technique that allows the obtention of an added value rosemary product where terpenes, compounds responsible for most of rosemary’s properties, are the most abundant. Two samples of locally acquired rosemary were sourced, essential oil was extracted, and characterization was then performed by Solid Phase Micro Extraction (SPME) and Gas Chromatography – Mass Spectrometry (GC-MS). As a comparison, a direct injection of a dilution of one of the essential oil samples was also performed. It was concluded that samples of essential oil obtained from both sources were entirely composed of terpenes or terpene derivatives. Besides, SPME was a superior method when compared to direct injection, since a larger number of volatiles was able to be identified.