The traditional consumption of citrus juices as a breakfast beverage has historical ties to obtaining one’s daily requirement of vitamin C. Vitamin C is one of the most important vitamins found in citrus juices, including orange juice. The aim of the study was to determine the effect of processing and storage on the content of vitamin C in orange juice varieties of ‘Valencia’ and ‘Navel’ of orange juice. The content of vitamin C (mg 100 gE-1) was determined with the iodine method in freshly squeezed orange juices and in pasteurized and packaged in aseptic bags orange juices of ‘Valencia ‘and ‘Navel’ variety, after processing and during 24 weeks of storage at 20 ± 1 °C and 5 ± 1 °C. The study results showed that the average content of vitamin C in freshly squeezed orange juice ‘Valencia’ was found at 33.56 mg 100gE-1 but in freshly squeezed orange juice ‘Navel’ the content of vitamin C was higher than in ‘Valencia’ variety orange juice average at 46.18 mg 100gE-1. The loss of vitamin C for both orange juices on average about 7% during processing The storage study showed that after 24 weeks of storage at 5 ± 1 °C and at 20 ± 1 °C, in pasteurized orange juice ‘Valencia’ the content of vitamin C decreased by 9.83% and 19.73 respectively. In the pasteurized orange juice ‘Navel’ the content of vitamin C decreased by 8.53% and 15.24% respectively.

The aim of the study was to assess the ability of pathogens metabolic repair from injury within 10 days of refrigerated storage of milk after high pressure treatment. Two pathogenic strains – Listeria monocytogenes ATCC 7644 (LM) and Escherichia coli ATCC 25922 (EC) were inoculated in ultrahigh-temperature treated (UHT) milk at concentration of about 107 CFU mLE-1 and treated at 400, 500, 550, and 600 MPa for 15 min with inlet temperatures 20 °C, and then stored at 4 ± 2 °C to evaluate survival and growth of pathogens. By increasing the applied pressure, an increased rate of the pathogens’ inactivation was achieved. After 10 days of storage, milk treated at 400 MPa showed growth over 3.5 log CFU mLE-1 of L. monocytogenes and 1.7 log CFU mLE-1 of E. coli. In 550 MPa and 600 MPa treated milk samples after 8 and 10 days of storage colony formation occurred (3 CFU mLE-1 (550 LM) and 2 CFU mLE-1 (550 EC, 600 LM and 600 EC)). Although high pressure treatment is effective method for reducing of pathogenic bacteria, the metabolic repair from injury of bacterial cells in milk during storage should be considered.